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The map label for this gene is mcl2 [H]

Identifier: 182678173

GI number: 182678173

Start: 1378082

End: 1378960

Strand: Direct

Name: mcl2 [H]

Synonym: Bind_1188

Alternate gene names: 182678173

Gene position: 1378082-1378960 (Clockwise)

Preceding gene: 182678171

Following gene: 182678174

Centisome position: 33.05

GC content: 60.52

Gene sequence:

>879_bases
ATGTCAATTCGACCCCGCCGGAGCATTCTCTATGTTCCAGGTGATAATGGGCGCGCCCTTGAAAAGGCGCGGGCGCTTGC
GGCCGATGGCTTTATTCTCGACCTTGAGGATGCGGTGGCACCAGACGCTAAGCTCCTGGCTCGTCAATCCGTCGTCGCGG
CGGTTGCTGAGAGGCGCTATGGCGCGCGGGAAACCATCGTTCGGATCAATGATTACAATACGCCTTGGGGGCGCGAAGAT
CTTGCCGCCGCCGCCGCCGCGGGGCCTGACGCCATCCTCATTCCGAAAGTGACCTGTCCCGGCGATATCATGCATGCCGC
CAAGGATCTGCGCGAAGCTGGCGCGCCTGATCACACCGCGCTCTGGGCGATGATGGAAACGCCGATCGCCATGCTGAATG
CGGATTCCATCCTGCGGACGGCGGCTGATCCGGCCTCGCGTTTGTCGGTCATCGTCATGGGTACGAATGATATCGCCAAG
GAAACGAGGGCCCGCCTGACGCCGGGGCGCGCGTCGATCATGACCTGGCTCTCCACCTGTGTCGTCGCCGCGCGGGCCTA
TTCGGTTGAAATCATTGATGGTGTCTATAATGATTTTTCCGATGCGGCAGGATTCCGCCAGGAGTGTTTGCAGGGGCGCG
ATCTCGGCATGGATGGTAAATCCCTCATCCATCCGAACCAGATCGCCACCTGCAACGAGATCTTTGCGCCGCCCGCCGAT
GAAATTCTTTGGGCGAGTAAGGTCATCTCAGCTTTCTCCGCGCCGGAAAATGCGGAGAAAGGGGTCTTGCAGGTCGAAGG
CCGCATGGTAGAGCGTCTACATGCGGAAATGGCGCGTCGGACCATGCAAATCGCCGAGGCCATCGCGGCCTTGGCGTGA

Upstream 100 bases:

>100_bases
CTGATCCGTGCCAATGGCACATCCCTTGCCGCGTCTGAGCCTATCCTCTTGTTTCATTTGTGCGTTGCAATAAAATGACG
AGCATAATCGGAGGCTCTTC

Downstream 100 bases:

>100_bases
TGAAAAGCGAGTAATTAAGCGTGACCACGAGAGTTTGAGTTGGGCGAAGGCCCGAAATATGGCATTGGGGCCAAGATCTG
GATGGTTCGTGCAAATGTTG

Product: Citryl-CoA lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSIRPRRSILYVPGDNGRALEKARALAADGFILDLEDAVAPDAKLLARQSVVAAVAERRYGARETIVRINDYNTPWGRED
LAAAAAAGPDAILIPKVTCPGDIMHAAKDLREAGAPDHTALWAMMETPIAMLNADSILRTAADPASRLSVIVMGTNDIAK
ETRARLTPGRASIMTWLSTCVVAARAYSVEIIDGVYNDFSDAAGFRQECLQGRDLGMDGKSLIHPNQIATCNEIFAPPAD
EILWASKVISAFSAPENAEKGVLQVEGRMVERLHAEMARRTMQIAEAIAALA

Sequences:

>Translated_292_residues
MSIRPRRSILYVPGDNGRALEKARALAADGFILDLEDAVAPDAKLLARQSVVAAVAERRYGARETIVRINDYNTPWGRED
LAAAAAAGPDAILIPKVTCPGDIMHAAKDLREAGAPDHTALWAMMETPIAMLNADSILRTAADPASRLSVIVMGTNDIAK
ETRARLTPGRASIMTWLSTCVVAARAYSVEIIDGVYNDFSDAAGFRQECLQGRDLGMDGKSLIHPNQIATCNEIFAPPAD
EILWASKVISAFSAPENAEKGVLQVEGRMVERLHAEMARRTMQIAEAIAALA
>Mature_291_residues
SIRPRRSILYVPGDNGRALEKARALAADGFILDLEDAVAPDAKLLARQSVVAAVAERRYGARETIVRINDYNTPWGREDL
AAAAAAGPDAILIPKVTCPGDIMHAAKDLREAGAPDHTALWAMMETPIAMLNADSILRTAADPASRLSVIVMGTNDIAKE
TRARLTPGRASIMTWLSTCVVAARAYSVEIIDGVYNDFSDAAGFRQECLQGRDLGMDGKSLIHPNQIATCNEIFAPPADE
ILWASKVISAFSAPENAEKGVLQVEGRMVERLHAEMARRTMQIAEAIAALA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=296, Percent_Identity=30.7432432432432, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI87081764, Length=288, Percent_Identity=34.0277777777778, Blast_Score=140, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=30.4347826086957, Blast_Score=136, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31419; Mature: 31288

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRPRRSILYVPGDNGRALEKARALAADGFILDLEDAVAPDAKLLARQSVVAAVAERRY
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEECHHHHCCCHHHHHHHHHHHHHHHHHC
GARETIVRINDYNTPWGREDLAAAAAAGPDAILIPKVTCPGDIMHAAKDLREAGAPDHTA
CCHHEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
LWAMMETPIAMLNADSILRTAADPASRLSVIVMGTNDIAKETRARLTPGRASIMTWLSTC
HHHHHHCCHHHHCHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
VVAARAYSVEIIDGVYNDFSDAAGFRQECLQGRDLGMDGKSLIHPNQIATCNEIFAPPAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCHH
EILWASKVISAFSAPENAEKGVLQVEGRMVERLHAEMARRTMQIAEAIAALA
HHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIRPRRSILYVPGDNGRALEKARALAADGFILDLEDAVAPDAKLLARQSVVAAVAERRY
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEECHHHHCCCHHHHHHHHHHHHHHHHHC
GARETIVRINDYNTPWGREDLAAAAAAGPDAILIPKVTCPGDIMHAAKDLREAGAPDHTA
CCHHEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
LWAMMETPIAMLNADSILRTAADPASRLSVIVMGTNDIAKETRARLTPGRASIMTWLSTC
HHHHHHCCHHHHCHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
VVAARAYSVEIIDGVYNDFSDAAGFRQECLQGRDLGMDGKSLIHPNQIATCNEIFAPPAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCHH
EILWASKVISAFSAPENAEKGVLQVEGRMVERLHAEMARRTMQIAEAIAALA
HHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA