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The map label for this gene is trmB [H]

Identifier: 182678049

GI number: 182678049

Start: 1219588

End: 1220325

Strand: Direct

Name: trmB [H]

Synonym: Bind_1063

Alternate gene names: 182678049

Gene position: 1219588-1220325 (Clockwise)

Preceding gene: 182678048

Following gene: 182678050

Centisome position: 29.25

GC content: 59.62

Gene sequence:

>738_bases
ATGCAGCAGCCTGCCTTGGACGAAAAAGACGTGCTCCCTCCCGCCCCCGAGGCGGAGGGTGCACCTTGCGAAACCACGCT
TTATGGTCGCCGCCGTGGCAAGAAGCTGCGGTCCTATCATTCCGCCTTGATGCGGGAAAAGCTCCCGGAAGTAGCCCTCG
ATCTCTCCCAGCCCGTCGCTTCCGTCACTGATCTTTTTCCGCACGCGCCCGAAGACGTCTGGCTGGAAATCGGCTTCGGG
GGTGGTGAACATCTGGCCGGCACGGCAGCTTCGCAGGCCAAAAAGGGGTTCATCGGCTGTGAAGCTTTTCATAATGGCGT
TGCAAAAATCCTGGTGCTGATCGAGGCGGAAGGCTTGCGCAATATCCGCGTCTTCGAAGGCGATGCGCGTAAGGTCTTGG
ATGCTTTGCCGCAGGCCAGTCTCGCCGGCATTTTTCTGCTTTATCCCGATCCCTGGCCAAAACGACGCCAGCGTAAACGT
CGTTTTCTTTCAGATGCCATGCTGGAACGTCTCGCCCGTGTCTTGCGGCCCGGCGCGCCTTTGCGTTTTGCCACGGATAT
TGATGATTATGCGGGCTGGACTCTGGCCCGCGTCCTCCGCTCCCCCGATTTCGACTGGCCTGCGCAGGAAGCCGTGGATT
GGCACAAGCCTTGGGAAGGCTGGACCGGAACGCGCTACGAGGCGAAGGCGCTGCGGCTGGGTCATCATCCGGCCTATTAT
ACGTTTATCCGGAAATGA

Upstream 100 bases:

>100_bases
GCCCGATGCCGATGGCGGTTTTTCCTGGGAAAAGACCGATCTCGTGGACAAGCTGAAGGCCGCGTTCGCCTAAACGCGAA
CGGAAAGCCGTTTGCAATCC

Downstream 100 bases:

>100_bases
CGCAGGAAGCCTTGATTGCATCCGCGTCCAAGGGCGTGGATGGGTCGTCTCCGATCGTGATCCAGATGAAGCTAGTTATG
TAAATTAATTATATACTGAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MQQPALDEKDVLPPAPEAEGAPCETTLYGRRRGKKLRSYHSALMREKLPEVALDLSQPVASVTDLFPHAPEDVWLEIGFG
GGEHLAGTAASQAKKGFIGCEAFHNGVAKILVLIEAEGLRNIRVFEGDARKVLDALPQASLAGIFLLYPDPWPKRRQRKR
RFLSDAMLERLARVLRPGAPLRFATDIDDYAGWTLARVLRSPDFDWPAQEAVDWHKPWEGWTGTRYEAKALRLGHHPAYY
TFIRK

Sequences:

>Translated_245_residues
MQQPALDEKDVLPPAPEAEGAPCETTLYGRRRGKKLRSYHSALMREKLPEVALDLSQPVASVTDLFPHAPEDVWLEIGFG
GGEHLAGTAASQAKKGFIGCEAFHNGVAKILVLIEAEGLRNIRVFEGDARKVLDALPQASLAGIFLLYPDPWPKRRQRKR
RFLSDAMLERLARVLRPGAPLRFATDIDDYAGWTLARVLRSPDFDWPAQEAVDWHKPWEGWTGTRYEAKALRLGHHPAYY
TFIRK
>Mature_245_residues
MQQPALDEKDVLPPAPEAEGAPCETTLYGRRRGKKLRSYHSALMREKLPEVALDLSQPVASVTDLFPHAPEDVWLEIGFG
GGEHLAGTAASQAKKGFIGCEAFHNGVAKILVLIEAEGLRNIRVFEGDARKVLDALPQASLAGIFLLYPDPWPKRRQRKR
RFLSDAMLERLARVLRPGAPLRFATDIDDYAGWTLARVLRSPDFDWPAQEAVDWHKPWEGWTGTRYEAKALRLGHHPAYY
TFIRK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=230, Percent_Identity=31.7391304347826, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27576; Mature: 27576

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQPALDEKDVLPPAPEAEGAPCETTLYGRRRGKKLRSYHSALMREKLPEVALDLSQPVA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTDLFPHAPEDVWLEIGFGGGEHLAGTAASQAKKGFIGCEAFHNGVAKILVLIEAEGLR
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCC
NIRVFEGDARKVLDALPQASLAGIFLLYPDPWPKRRQRKRRFLSDAMLERLARVLRPGAP
EEEEEECHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LRFATDIDDYAGWTLARVLRSPDFDWPAQEAVDWHKPWEGWTGTRYEAKALRLGHHPAYY
EEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHE
TFIRK
EEECC
>Mature Secondary Structure
MQQPALDEKDVLPPAPEAEGAPCETTLYGRRRGKKLRSYHSALMREKLPEVALDLSQPVA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTDLFPHAPEDVWLEIGFGGGEHLAGTAASQAKKGFIGCEAFHNGVAKILVLIEAEGLR
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCC
NIRVFEGDARKVLDALPQASLAGIFLLYPDPWPKRRQRKRRFLSDAMLERLARVLRPGAP
EEEEEECHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LRFATDIDDYAGWTLARVLRSPDFDWPAQEAVDWHKPWEGWTGTRYEAKALRLGHHPAYY
EEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHE
TFIRK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA