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The map label for this gene is metK [H]

Identifier: 182678048

GI number: 182678048

Start: 1218364

End: 1219560

Strand: Direct

Name: metK [H]

Synonym: Bind_1062

Alternate gene names: 182678048

Gene position: 1218364-1219560 (Clockwise)

Preceding gene: 182678047

Following gene: 182678049

Centisome position: 29.22

GC content: 59.65

Gene sequence:

>1197_bases
GTGGCACGCGCGAATTATCTTTTCACCAGTGAATCGGTTTCGGAAGGTCATCCGGACAAGGTCTGCGATCGCATTTCGGA
CGAGATCGTCGATCTGTTCTTCCGCGAAGGTGAAAAGGCGGGCATTGATCCCTATCAGATCCGTGTCGCCTGCGAGAATC
TTGCGACCACGAATCGGCTGGTGATCGCTGGCGAGGTTCGCGGGCCCGCCCTATCCCAAGAAACGATCATCGAAACCGCG
CGCAAGGCCATCAAGGATATTGGCTACGAGCAAAAGGGCTTCCATTGGCGCGATGTCGCCGTCGAGGTCTTGCTGCATGC
GCAATCCGCCGATATCGCGCAGGGCGTCGATTCCTCCGGCAACAAGGATGAGGGCGCAGGCGATCAAGGTGTCATGTTTG
GCTATGCCTGCACAGAGACACCGGAGCTGATGCCGGCGCCGCTCTATTATTCGCACAAGATCCTCGAAGTCCTGGCTGCC
GCGCGCAAGGCCGGTATCGGCGATGCCGCCAAGCTTGGCCCGGATGCCAAGAGCCAGGTGACGGTCCGCTATGTCGACGG
CAAGCCGGTTGGTGTGACGCAGATCGTGCTTTCGACGCAGCATATCGATGAGACTCTGACCTCCGAGGATGTGCGCGCGA
TTGTCGAGCCCTACATCCGCAAAACCTTGCCGCAAGGCTGGATTACTGAGGAAACCGTCTGGCACGTCAATCCGACCGGC
AAATTCGTCATTGGCGGTCCGGATGGTGATACGGGCCTGACTGGGCGCAAGATTATCGTCGACACTTACGGCGGTGCCGC
ACCGCATGGCGGTGGTGCCTTCTCCGGCAAAGACCCGACCAAGGTTGATCGCTCGGCGGCTTACGCCGCGCGTTATCTTG
CCAAAAATGTTGTCGCGGCGGGTCTCGCCGATCGCGTGACCATCCAGCTCGCCTATGCCATCGGTGTGGCGGAACCGCTG
TCGATCTATGTCGATACTCATGGGACGGGCAAGGTTCCCGAGGACAAGCTCGAGACGGTCCTGTCGCAGGTCATGCGGCT
TTCGCCGCGTGGTATTCGCGAACATCTCCAGCTCAACAAGCCGATCTATGCGCGCACCTCGGCTTATGGTCATTTCGGCC
GGACGCCCGATGCCGATGGCGGTTTTTCCTGGGAAAAGACCGATCTCGTGGACAAGCTGAAGGCCGCGTTCGCCTAA

Upstream 100 bases:

>100_bases
ATTTTAGGCCGTGTCGCGGACGACGAAAGCATCATGCCAAAAGGTTGGTTCCTTTTTGGATGCTCCATCCTTGACGAAAC
AGAGCCCAGGAGACGATTGG

Downstream 100 bases:

>100_bases
ACGCGAACGGAAAGCCGTTTGCAATCCATGCAGCAGCCTGCCTTGGACGAAAAAGACGTGCTCCCTCCCGCCCCCGAGGC
GGAGGGTGCACCTTGCGAAA

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MARANYLFTSESVSEGHPDKVCDRISDEIVDLFFREGEKAGIDPYQIRVACENLATTNRLVIAGEVRGPALSQETIIETA
RKAIKDIGYEQKGFHWRDVAVEVLLHAQSADIAQGVDSSGNKDEGAGDQGVMFGYACTETPELMPAPLYYSHKILEVLAA
ARKAGIGDAAKLGPDAKSQVTVRYVDGKPVGVTQIVLSTQHIDETLTSEDVRAIVEPYIRKTLPQGWITEETVWHVNPTG
KFVIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLADRVTIQLAYAIGVAEPL
SIYVDTHGTGKVPEDKLETVLSQVMRLSPRGIREHLQLNKPIYARTSAYGHFGRTPDADGGFSWEKTDLVDKLKAAFA

Sequences:

>Translated_398_residues
MARANYLFTSESVSEGHPDKVCDRISDEIVDLFFREGEKAGIDPYQIRVACENLATTNRLVIAGEVRGPALSQETIIETA
RKAIKDIGYEQKGFHWRDVAVEVLLHAQSADIAQGVDSSGNKDEGAGDQGVMFGYACTETPELMPAPLYYSHKILEVLAA
ARKAGIGDAAKLGPDAKSQVTVRYVDGKPVGVTQIVLSTQHIDETLTSEDVRAIVEPYIRKTLPQGWITEETVWHVNPTG
KFVIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLADRVTIQLAYAIGVAEPL
SIYVDTHGTGKVPEDKLETVLSQVMRLSPRGIREHLQLNKPIYARTSAYGHFGRTPDADGGFSWEKTDLVDKLKAAFA
>Mature_397_residues
ARANYLFTSESVSEGHPDKVCDRISDEIVDLFFREGEKAGIDPYQIRVACENLATTNRLVIAGEVRGPALSQETIIETAR
KAIKDIGYEQKGFHWRDVAVEVLLHAQSADIAQGVDSSGNKDEGAGDQGVMFGYACTETPELMPAPLYYSHKILEVLAAA
RKAGIGDAAKLGPDAKSQVTVRYVDGKPVGVTQIVLSTQHIDETLTSEDVRAIVEPYIRKTLPQGWITEETVWHVNPTGK
FVIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLADRVTIQLAYAIGVAEPLS
IYVDTHGTGKVPEDKLETVLSQVMRLSPRGIREHLQLNKPIYARTSAYGHFGRTPDADGGFSWEKTDLVDKLKAAFA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=392, Percent_Identity=47.4489795918367, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=48.0719794344473, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=52.6582278481013, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538498, Length=393, Percent_Identity=45.5470737913486, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17538494, Length=393, Percent_Identity=45.29262086514, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=45.9390862944162, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17551082, Length=390, Percent_Identity=44.6153846153846, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=42.1994884910486, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI32565911, Length=334, Percent_Identity=45.2095808383234, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI25145635, Length=335, Percent_Identity=45.6716417910448, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=46.1794019933555, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI32565909, Length=90, Percent_Identity=41.1111111111111, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=391, Percent_Identity=47.8260869565217, Blast_Score=313, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=46.1538461538462, Blast_Score=309, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24580527, Length=391, Percent_Identity=47.0588235294118, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24580525, Length=391, Percent_Identity=47.0588235294118, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=47.0588235294118, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=47.0588235294118, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=47.0588235294118, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=47.0588235294118, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=47.0588235294118, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=47.0588235294118, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580541, Length=390, Percent_Identity=44.6153846153846, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24580539, Length=129, Percent_Identity=40.3100775193798, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42925; Mature: 42794

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARANYLFTSESVSEGHPDKVCDRISDEIVDLFFREGEKAGIDPYQIRVACENLATTNRL
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCEE
VIAGEVRGPALSQETIIETARKAIKDIGYEQKGFHWRDVAVEVLLHAQSADIAQGVDSSG
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
NKDEGAGDQGVMFGYACTETPELMPAPLYYSHKILEVLAAARKAGIGDAAKLGPDAKSQV
CCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEE
TVRYVDGKPVGVTQIVLSTQHIDETLTSEDVRAIVEPYIRKTLPQGWITEETVWHVNPTG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
KFVIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLADRVTIQLAYAIGVAEPLSIYVDTHGTGKVPEDKLETVLSQVMRLSPRGIREHLQLNK
CCCHHEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCC
PIYARTSAYGHFGRTPDADGGFSWEKTDLVDKLKAAFA
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ARANYLFTSESVSEGHPDKVCDRISDEIVDLFFREGEKAGIDPYQIRVACENLATTNRL
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCEE
VIAGEVRGPALSQETIIETARKAIKDIGYEQKGFHWRDVAVEVLLHAQSADIAQGVDSSG
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
NKDEGAGDQGVMFGYACTETPELMPAPLYYSHKILEVLAAARKAGIGDAAKLGPDAKSQV
CCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEE
TVRYVDGKPVGVTQIVLSTQHIDETLTSEDVRAIVEPYIRKTLPQGWITEETVWHVNPTG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
KFVIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLADRVTIQLAYAIGVAEPLSIYVDTHGTGKVPEDKLETVLSQVMRLSPRGIREHLQLNK
CCCHHEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCC
PIYARTSAYGHFGRTPDADGGFSWEKTDLVDKLKAAFA
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA