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The map label for this gene is dusA [H]

Identifier: 182677984

GI number: 182677984

Start: 1144142

End: 1145152

Strand: Direct

Name: dusA [H]

Synonym: Bind_0998

Alternate gene names: 182677984

Gene position: 1144142-1145152 (Clockwise)

Preceding gene: 182677983

Following gene: 182677986

Centisome position: 27.44

GC content: 60.73

Gene sequence:

>1011_bases
ATGAAGCAATTTCAATCGTATAGATTTTCCGTCGCGCCGATGATGGAATGGACCGATCGGCATTGCCGCGTTTTTCATCG
GCTTTTGTCGCGTCGGGCGCTCCTTTATACGGAGATGATTACAACGGCGGCATTGCTGCATGGTCACGTCGATCGTTTGC
TTGGCTTTGATGATGTCGAACATCCCGTTGCTGTGCAACTCGGCGGTTCTGAACCGCGCGATCTGGCGGCGGCGGCACGC
ATTTGCGCGGATTTCGGCTATGACGAAATCAATCTCAATATCGGCTGTCCCTCGGAGCGCGTGCAGAACGGGGCTTTCGG
AGCCTGTCTCATGCTGGAACCGGAGCTGGTTGGGGATTGTGTCGCGGCGATCAAGGCGAGCGTTTCCTTGCCGGTCACGG
TCAAATGTCGGATCGGTGTCGATGACCAGGACCCGGAGGAAGCCTTGGGGACTTTGAGCGCGGCGATCATTGCCGCGGGC
GTCGACGGGATTATCGTCCATGCCCGCAAGGCCTGGCTCAAGGGTCTGTCGCCGCGCCAGAACCGCGATGTGCCCCCACT
CGATTATCCGCTCGTTCATGCCTTGAAGCGGACCTATCCCGAGATACCGATCATGATTAATGGTGGGCTGGTGACGCATG
AATCCATGCAGGAGCAGCTTCGCCATGTCGATGGGGTGATGGTCGGGCGCCAAGCTTATCAGGAGCCGGCCATGCTGCTC
TCGGTCGATCCGCTGTTTTTTGGCGAGGAGGCTCCGCTCACCGACGCCACGGATGTAGTCGAGGCTTTCATTCCCTATGT
CGAAGCGCGGCGGGCCGAAGGGGTGCCGCTGCACTCCATGACACGGCATATTCTGGGCCTGTTCAACGGGCGGCCGGGCG
CGCGGGCCTTTCGGCGCCATCTCGCCACGGAAGGTCTGGCACGTGATGCGGGCGCCGACGTTCTGCGGGCCGCCGCTGCG
CATGTGGTCAAGGCGAGGAGCTTTCGGCCCGTCGAGGCTGTATCAGGCTGA

Upstream 100 bases:

>100_bases
ATGAGCGGCGAGCTAAATTATGGCTGTGGTTCGGGCGCATCGCAGGATGGCGTAAAAGAGTGCAAAGGATGCGCGCTCAT
GGCTTTGAGATTACTCAAAA

Downstream 100 bases:

>100_bases
AAAAGGCTCAGGTGGCCCGGCCCAAGCGGCCCGCGAGATCTTGAATATATTGCCAGGCGGTACGGCCGGAACGGGCGCCG
CGCGTGATTGCCCATTCGAG

Product: YjbN family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKQFQSYRFSVAPMMEWTDRHCRVFHRLLSRRALLYTEMITTAALLHGHVDRLLGFDDVEHPVAVQLGGSEPRDLAAAAR
ICADFGYDEINLNIGCPSERVQNGAFGACLMLEPELVGDCVAAIKASVSLPVTVKCRIGVDDQDPEEALGTLSAAIIAAG
VDGIIVHARKAWLKGLSPRQNRDVPPLDYPLVHALKRTYPEIPIMINGGLVTHESMQEQLRHVDGVMVGRQAYQEPAMLL
SVDPLFFGEEAPLTDATDVVEAFIPYVEARRAEGVPLHSMTRHILGLFNGRPGARAFRRHLATEGLARDAGADVLRAAAA
HVVKARSFRPVEAVSG

Sequences:

>Translated_336_residues
MKQFQSYRFSVAPMMEWTDRHCRVFHRLLSRRALLYTEMITTAALLHGHVDRLLGFDDVEHPVAVQLGGSEPRDLAAAAR
ICADFGYDEINLNIGCPSERVQNGAFGACLMLEPELVGDCVAAIKASVSLPVTVKCRIGVDDQDPEEALGTLSAAIIAAG
VDGIIVHARKAWLKGLSPRQNRDVPPLDYPLVHALKRTYPEIPIMINGGLVTHESMQEQLRHVDGVMVGRQAYQEPAMLL
SVDPLFFGEEAPLTDATDVVEAFIPYVEARRAEGVPLHSMTRHILGLFNGRPGARAFRRHLATEGLARDAGADVLRAAAA
HVVKARSFRPVEAVSG
>Mature_336_residues
MKQFQSYRFSVAPMMEWTDRHCRVFHRLLSRRALLYTEMITTAALLHGHVDRLLGFDDVEHPVAVQLGGSEPRDLAAAAR
ICADFGYDEINLNIGCPSERVQNGAFGACLMLEPELVGDCVAAIKASVSLPVTVKCRIGVDDQDPEEALGTLSAAIIAAG
VDGIIVHARKAWLKGLSPRQNRDVPPLDYPLVHALKRTYPEIPIMINGGLVTHESMQEQLRHVDGVMVGRQAYQEPAMLL
SVDPLFFGEEAPLTDATDVVEAFIPYVEARRAEGVPLHSMTRHILGLFNGRPGARAFRRHLATEGLARDAGADVLRAAAA
HVVKARSFRPVEAVSG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=309, Percent_Identity=28.4789644012945, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=55.1612903225806, Blast_Score=347, Evalue=4e-97,
Organism=Escherichia coli, GI1788462, Length=180, Percent_Identity=30.5555555555556, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789660, Length=312, Percent_Identity=25, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=251, Percent_Identity=28.6852589641434, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=267, Percent_Identity=21.7228464419476, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.1410788381743, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580595, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19920448, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36724; Mature: 36724

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFQSYRFSVAPMMEWTDRHCRVFHRLLSRRALLYTEMITTAALLHGHVDRLLGFDDVE
CCCHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HPVAVQLGGSEPRDLAAAARICADFGYDEINLNIGCPSERVQNGAFGACLMLEPELVGDC
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEECHHHHHHH
VAAIKASVSLPVTVKCRIGVDDQDPEEALGTLSAAIIAAGVDGIIVHARKAWLKGLSPRQ
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCC
NRDVPPLDYPLVHALKRTYPEIPIMINGGLVTHESMQEQLRHVDGVMVGRQAYQEPAMLL
CCCCCCCCHHHHHHHHHHCCCCCEEEECCEECHHHHHHHHHHHHHHHHHHHHHHCCCEEE
SVDPLFFGEEAPLTDATDVVEAFIPYVEARRAEGVPLHSMTRHILGLFNGRPGARAFRRH
EECCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LATEGLARDAGADVLRAAAAHVVKARSFRPVEAVSG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKQFQSYRFSVAPMMEWTDRHCRVFHRLLSRRALLYTEMITTAALLHGHVDRLLGFDDVE
CCCHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HPVAVQLGGSEPRDLAAAARICADFGYDEINLNIGCPSERVQNGAFGACLMLEPELVGDC
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEECHHHHHHH
VAAIKASVSLPVTVKCRIGVDDQDPEEALGTLSAAIIAAGVDGIIVHARKAWLKGLSPRQ
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCC
NRDVPPLDYPLVHALKRTYPEIPIMINGGLVTHESMQEQLRHVDGVMVGRQAYQEPAMLL
CCCCCCCCHHHHHHHHHHCCCCCEEEECCEECHHHHHHHHHHHHHHHHHHHHHHCCCEEE
SVDPLFFGEEAPLTDATDVVEAFIPYVEARRAEGVPLHSMTRHILGLFNGRPGARAFRRH
EECCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LATEGLARDAGADVLRAAAAHVVKARSFRPVEAVSG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]