Click here to switch to the map view.

The map label for this gene is nuoE2 [H]

Identifier: 182677975

GI number: 182677975

Start: 1133046

End: 1133519

Strand: Direct

Name: nuoE2 [H]

Synonym: Bind_0988

Alternate gene names: 182677975

Gene position: 1133046-1133519 (Clockwise)

Preceding gene: 182677973

Following gene: 182677976

Centisome position: 27.17

GC content: 60.34

Gene sequence:

>474_bases
ATGGCATTCCACGAGCCATGGAATGACGCCGTTGCTGCGACGATCATCGCCCAGCACAAGGCGCAAGAAGGGGCGACTCT
TCCCATCTTCCACGCGCTTCAAAAGGCTTTTGGCTATGTGCCCGCCGAAGCGGTTCCGATGATCGCCGAGGCACTGAACC
TGTCCCGCGCGGAAGTTCATGGTGTTCTGACCTTTTATCATGATTTCCGCAGCGAGCCGCCGGGCTGCCATATATTGAAG
CTGTGCCGTGCCGAAGCCTGTCAGGCGGCAGGGGGCGACCATCTGGCCACGGGAGTCGAGCGGCAACTCGGCATTAAGCT
CGGTGAGACCACACCAGACCAGCAGGTGACTCTTGAACCGGTCTTTTGCCTCGGTCTTTGCGCTGTAGCCCCCTCCGCCA
TGCTCGATGATCAGCCGATCGGTCGAGTAGATGCGGCCAGGCTCGATGCCCTCCTGAACGAGGTGCGCTCATGA

Upstream 100 bases:

>100_bases
AACGAAAGAGCTTTATTGATCCCCTTCGAGGGACGTTCCATAGTTTTCCGCACAGCCCGTTGCTGGACAATGATCATAAT
CATGAGGAGGATTCCCCCTG

Downstream 100 bases:

>100_bases
CGATACGCTTCTTCATTTCCCGTGATGCGGCGTCTCTGGCAGTCGGAGCCGATGCCATAGCCAAAGCCTTGCAGCAAGCA
GCGGATACAAAGGGCCTTGC

Product: NADH dehydrogenase (ubiquinone) 24 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAFHEPWNDAVAATIIAQHKAQEGATLPIFHALQKAFGYVPAEAVPMIAEALNLSRAEVHGVLTFYHDFRSEPPGCHILK
LCRAEACQAAGGDHLATGVERQLGIKLGETTPDQQVTLEPVFCLGLCAVAPSAMLDDQPIGRVDAARLDALLNEVRS

Sequences:

>Translated_157_residues
MAFHEPWNDAVAATIIAQHKAQEGATLPIFHALQKAFGYVPAEAVPMIAEALNLSRAEVHGVLTFYHDFRSEPPGCHILK
LCRAEACQAAGGDHLATGVERQLGIKLGETTPDQQVTLEPVFCLGLCAVAPSAMLDDQPIGRVDAARLDALLNEVRS
>Mature_156_residues
AFHEPWNDAVAATIIAQHKAQEGATLPIFHALQKAFGYVPAEAVPMIAEALNLSRAEVHGVLTFYHDFRSEPPGCHILKL
CRAEACQAAGGDHLATGVERQLGIKLGETTPDQQVTLEPVFCLGLCAVAPSAMLDDQPIGRVDAARLDALLNEVRS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=149, Percent_Identity=30.8724832214765, Blast_Score=90, Evalue=9e-19,
Organism=Escherichia coli, GI1788621, Length=113, Percent_Identity=35.3982300884956, Blast_Score=81, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=139, Percent_Identity=31.6546762589928, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18859877, Length=136, Percent_Identity=30.8823529411765, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355353, Length=135, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 16835; Mature: 16704

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFHEPWNDAVAATIIAQHKAQEGATLPIFHALQKAFGYVPAEAVPMIAEALNLSRAEVH
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
GVLTFYHDFRSEPPGCHILKLCRAEACQAAGGDHLATGVERQLGIKLGETTPDQQVTLEP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEECCCCCCCCEEHHH
VFCLGLCAVAPSAMLDDQPIGRVDAARLDALLNEVRS
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFHEPWNDAVAATIIAQHKAQEGATLPIFHALQKAFGYVPAEAVPMIAEALNLSRAEVH
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHH
GVLTFYHDFRSEPPGCHILKLCRAEACQAAGGDHLATGVERQLGIKLGETTPDQQVTLEP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEECCCCCCCCEEHHH
VFCLGLCAVAPSAMLDDQPIGRVDAARLDALLNEVRS
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]