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The map label for this gene is nuoF2 [H]

Identifier: 182677976

GI number: 182677976

Start: 1133516

End: 1135066

Strand: Direct

Name: nuoF2 [H]

Synonym: Bind_0989

Alternate gene names: 182677976

Gene position: 1133516-1135066 (Clockwise)

Preceding gene: 182677975

Following gene: 182677977

Centisome position: 27.18

GC content: 60.03

Gene sequence:

>1551_bases
ATGACGATACGCTTCTTCATTTCCCGTGATGCGGCGTCTCTGGCAGTCGGAGCCGATGCCATAGCCAAAGCCTTGCAGCA
AGCAGCGGATACAAAGGGCCTTGCCATCGAGATCATACGCACAGGTTCACGCGGGCTGTTCTGGCTGGAGCCGATGATCG
AGGTGGAAACTCCTCAGGGACGTATCGCTTATGGTCCTCTAGCGCCGGAAAACGTGGCTGAGCTTCTTACAGATGGTCTC
ATGAACGGTGAACCGCATCCCTTGTGCCTTGGGCGGCCGGAAGCCATTCCCTATCTCGCCAAACAACAGCGTCTCACTTT
CGCGCGTTGCGGCATCGTCGATCCTCGCTCCCTCGAAGATTATCTGGCGCATGGCGGTTATCGTGGCTTGGCCCGGGCCC
TTGATCTTGGACCAGCCGCGATGATCGAGGAAGTGGTCGAGTCGGGATTGCGTGGCCGTGGCGGTGCGGGTTTTCCGACT
GGCATCAAATGGCGGACCGTGGCGCAGACAAAGCCGGGGCAGAAATATATCGTTTGCAATGCCGATGAAGGCGATAGCGG
GACCTTTTCCGATCGCATGATCATGGAAGGCGATCCCTTCGTCCTGATCGAAGGGATGACGATCGCGGGCATCGCGGTCG
GCGCGACGAAGGGCTATATTTATATCCGCTCGGAATATCCGCATGCCGTCCATGCGATGGAGGCGGCGATCGAGGCCGCG
CGCCGCGCCGATTATCTCGGGTCCAATGTGGCGGGCTCGGAATGGGCCTTCGACATTGAGGTGCGCGTGGGCGCTGGCGC
CTATGTCTGCGGCGAAGAAACCGCCTTGCTCGAAAGCCTCGAAGGCAAACGTGGCCTTGTCCGTGCCAAGCCGCCGCTGC
CTGCGCATGTCGGCCTGTTCGGCTGTCCGACTGTCATCAATAATGTCATGTCTCTTGCGACTGTGCCCTTCATTCTGGCG
CAAGGCGCCAAGACTTACCAGGATTATGGCATGGGCCGCTCGCGCGGGACGATGCCGATTCAACTCGCTGGTAATGTCAA
GCATGGCGGCTTGGTCGAGATCGCCTTTGGCGTCACGCTCGGCGAACTCGTCGAGGGTTTTGGCGGCGGTACGCGCAGCG
GCCGGCCGGTGCGCGCGGTCCAAGTCGGTGGTCCGCTCGGCGCCTATTTTCCCGTCGATCTTTTCGACACACCGTTCGAT
TACGAGGCTTTCGCCGCTCGCGATGGCTTGATCGGCCACGGCGGCATCGTCGTCTTCGACGATACGGTGGACATGCTGAA
ACAGGCGCGTTTCGCGATGGAATTTTGCGCTCATGAGAGCTGCGGCAAATGTACACCCTGCCGCATCGGCTCGACGCGAG
GCGCGGAAACCCTGGACAAGATTGCCCATGGCATGGAGGCTGAGCACAATCTCGCCCTCGTCAAGGACCTTTGCGAGACG
ATGAAATTCGGCTCGCTCTGTGCGCTTGGTGGTTTCACACCTTATCCCGTCATGAGTGCGCTGACGCATTTTCAGGACGA
TTTCACACCCGTGCGGCACGCCGCCGAATAG

Upstream 100 bases:

>100_bases
TCGGTCTTTGCGCTGTAGCCCCCTCCGCCATGCTCGATGATCAGCCGATCGGTCGAGTAGATGCGGCCAGGCTCGATGCC
CTCCTGAACGAGGTGCGCTC

Downstream 100 bases:

>100_bases
AACCAGGGATCGCGCTTTTCGATCCTCGATAGAAAACAATCAGGAGACGCCCAGCATGTCGCTCATCCACGAGATCGACT
ACGGGACCCCGCTTTCGACA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTIRFFISRDAASLAVGADAIAKALQQAADTKGLAIEIIRTGSRGLFWLEPMIEVETPQGRIAYGPLAPENVAELLTDGL
MNGEPHPLCLGRPEAIPYLAKQQRLTFARCGIVDPRSLEDYLAHGGYRGLARALDLGPAAMIEEVVESGLRGRGGAGFPT
GIKWRTVAQTKPGQKYIVCNADEGDSGTFSDRMIMEGDPFVLIEGMTIAGIAVGATKGYIYIRSEYPHAVHAMEAAIEAA
RRADYLGSNVAGSEWAFDIEVRVGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHVGLFGCPTVINNVMSLATVPFILA
QGAKTYQDYGMGRSRGTMPIQLAGNVKHGGLVEIAFGVTLGELVEGFGGGTRSGRPVRAVQVGGPLGAYFPVDLFDTPFD
YEAFAARDGLIGHGGIVVFDDTVDMLKQARFAMEFCAHESCGKCTPCRIGSTRGAETLDKIAHGMEAEHNLALVKDLCET
MKFGSLCALGGFTPYPVMSALTHFQDDFTPVRHAAE

Sequences:

>Translated_516_residues
MTIRFFISRDAASLAVGADAIAKALQQAADTKGLAIEIIRTGSRGLFWLEPMIEVETPQGRIAYGPLAPENVAELLTDGL
MNGEPHPLCLGRPEAIPYLAKQQRLTFARCGIVDPRSLEDYLAHGGYRGLARALDLGPAAMIEEVVESGLRGRGGAGFPT
GIKWRTVAQTKPGQKYIVCNADEGDSGTFSDRMIMEGDPFVLIEGMTIAGIAVGATKGYIYIRSEYPHAVHAMEAAIEAA
RRADYLGSNVAGSEWAFDIEVRVGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHVGLFGCPTVINNVMSLATVPFILA
QGAKTYQDYGMGRSRGTMPIQLAGNVKHGGLVEIAFGVTLGELVEGFGGGTRSGRPVRAVQVGGPLGAYFPVDLFDTPFD
YEAFAARDGLIGHGGIVVFDDTVDMLKQARFAMEFCAHESCGKCTPCRIGSTRGAETLDKIAHGMEAEHNLALVKDLCET
MKFGSLCALGGFTPYPVMSALTHFQDDFTPVRHAAE
>Mature_515_residues
TIRFFISRDAASLAVGADAIAKALQQAADTKGLAIEIIRTGSRGLFWLEPMIEVETPQGRIAYGPLAPENVAELLTDGLM
NGEPHPLCLGRPEAIPYLAKQQRLTFARCGIVDPRSLEDYLAHGGYRGLARALDLGPAAMIEEVVESGLRGRGGAGFPTG
IKWRTVAQTKPGQKYIVCNADEGDSGTFSDRMIMEGDPFVLIEGMTIAGIAVGATKGYIYIRSEYPHAVHAMEAAIEAAR
RADYLGSNVAGSEWAFDIEVRVGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHVGLFGCPTVINNVMSLATVPFILAQ
GAKTYQDYGMGRSRGTMPIQLAGNVKHGGLVEIAFGVTLGELVEGFGGGTRSGRPVRAVQVGGPLGAYFPVDLFDTPFDY
EAFAARDGLIGHGGIVVFDDTVDMLKQARFAMEFCAHESCGKCTPCRIGSTRGAETLDKIAHGMEAEHNLALVKDLCETM
KFGSLCALGGFTPYPVMSALTHFQDDFTPVRHAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=394, Percent_Identity=38.5786802030457, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI20149568, Length=394, Percent_Identity=38.5786802030457, Blast_Score=271, Evalue=9e-73,
Organism=Escherichia coli, GI1788620, Length=398, Percent_Identity=38.1909547738693, Blast_Score=284, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17531727, Length=395, Percent_Identity=38.9873417721519, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI19920794, Length=415, Percent_Identity=37.8313253012048, Blast_Score=269, Evalue=4e-72,
Organism=Drosophila melanogaster, GI45550452, Length=391, Percent_Identity=36.3171355498721, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24653819, Length=370, Percent_Identity=31.0810810810811, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=28.9156626506024, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55021; Mature: 54890

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRFFISRDAASLAVGADAIAKALQQAADTKGLAIEIIRTGSRGLFWLEPMIEVETPQG
CEEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCEEEEECCCC
RIAYGPLAPENVAELLTDGLMNGEPHPLCLGRPEAIPYLAKQQRLTFARCGIVDPRSLED
EEEECCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHCCHHHHCCCCCCHHHHH
YLAHGGYRGLARALDLGPAAMIEEVVESGLRGRGGAGFPTGIKWRTVAQTKPGQKYIVCN
HHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
ADEGDSGTFSDRMIMEGDPFVLIEGMTIAGIAVGATKGYIYIRSEYPHAVHAMEAAIEAA
CCCCCCCCCCCCEEECCCCEEEEECCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHH
RRADYLGSNVAGSEWAFDIEVRVGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHVGLF
HHHHHCCCCCCCCCEEEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCE
GCPTVINNVMSLATVPFILAQGAKTYQDYGMGRSRGTMPIQLAGNVKHGGLVEIAFGVTL
ECHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCH
GELVEGFGGGTRSGRPVRAVQVGGPLGAYFPVDLFDTPFDYEAFAARDGLIGHGGIVVFD
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCEEEEC
DTVDMLKQARFAMEFCAHESCGKCTPCRIGSTRGAETLDKIAHGMEAEHNLALVKDLCET
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
MKFGSLCALGGFTPYPVMSALTHFQDDFTPVRHAAE
HCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
TIRFFISRDAASLAVGADAIAKALQQAADTKGLAIEIIRTGSRGLFWLEPMIEVETPQG
EEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCEEEEECCCC
RIAYGPLAPENVAELLTDGLMNGEPHPLCLGRPEAIPYLAKQQRLTFARCGIVDPRSLED
EEEECCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHCCHHHHCCCCCCHHHHH
YLAHGGYRGLARALDLGPAAMIEEVVESGLRGRGGAGFPTGIKWRTVAQTKPGQKYIVCN
HHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
ADEGDSGTFSDRMIMEGDPFVLIEGMTIAGIAVGATKGYIYIRSEYPHAVHAMEAAIEAA
CCCCCCCCCCCCEEECCCCEEEEECCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHH
RRADYLGSNVAGSEWAFDIEVRVGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHVGLF
HHHHHCCCCCCCCCEEEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCE
GCPTVINNVMSLATVPFILAQGAKTYQDYGMGRSRGTMPIQLAGNVKHGGLVEIAFGVTL
ECHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCH
GELVEGFGGGTRSGRPVRAVQVGGPLGAYFPVDLFDTPFDYEAFAARDGLIGHGGIVVFD
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCEEEEC
DTVDMLKQARFAMEFCAHESCGKCTPCRIGSTRGAETLDKIAHGMEAEHNLALVKDLCET
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
MKFGSLCALGGFTPYPVMSALTHFQDDFTPVRHAAE
HCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]