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The map label for this gene is ycdJ [C]

Identifier: 182677958

GI number: 182677958

Start: 1110772

End: 1111635

Strand: Direct

Name: ycdJ [C]

Synonym: Bind_0971

Alternate gene names: 182677958

Gene position: 1110772-1111635 (Clockwise)

Preceding gene: 182677957

Following gene: 182677959

Centisome position: 26.64

GC content: 56.13

Gene sequence:

>864_bases
ATGATTGAAATCCGCCACAAGACGGTCCATGCCAATGGCATACGCCAGCACTATCTTGAGGTTGGCAATGGTCCGCCCAT
CGTATTGCTCCATGGATTTCCTGAAACGAGCTATGCATGGCGCCATCAGATTCCCGTGCTCGCCGAACATTACCGGGTGA
TTGCGCCGGACCTGCGCGGTTATGGCGAAACGGACAAGCCCGCGGCAGGTTATGACAAACGCACGATGGCGCTCGACATC
GTCGCATTGCTCAAAGCTCTCGATATCCCCAAGATCGCGCTCATCGGCCATGATCGTGGTGCGCGTGTAGCCACGCGTTT
CGCCAAGGATCATCCAGCTCTCCTTGATCGCTTGGTCGTGATGGATAATGTGCCAACACGGATCGTCGCACGAAGCGTAA
ACGCGCAAGTTGCCAAGGCATATTGGTTTTTCTTCTTTCACCTCGTGCCGGATTTACCCGAAGCGCTGATCGCTGGCCGG
GAGGATTTGTGGCTACGGCATTTCTTCTCCGATTGGTGTTACAACCCTCACACAATCTCGGGGGAAGCGTTCGACACTTA
TGTTCAGGCCTATCGGCGGCCCGGTGCGGTACGCGGGGCGATGGCGGATTACCGGGCCAATCTCGAGGATAATGCGCAAG
ATCAGATCGATGCCGACGTGAAAATCACCTGTCCCGTCCTTTCGCTCTGGGGAGAGGATTTCGAAGCCGTCGGCAAAATG
TTCGATATGCCTTCCATCTGGGCCGAGATGGCACACAATCTTCGCGCCGAACCGATCGCGCAATGTGGTCACCTGCCACA
TGAGGAACAGCCCGAGCGCGTGAACAAGCTGCTGCTGGAATTTCTCGATGGTTGGGCTGGTTGA

Upstream 100 bases:

>100_bases
GTACGGTACTGACGACTTTCCGTGCGGATTGAACATGATGAAAAATCGCTCGGCAAGAGAATCTGTTTCCTTGCACTTGC
ATGGTATGAGGGATTTTCCG

Downstream 100 bases:

>100_bases
ACCGTATCAACGTCGAACCTGACATCAGATGGAGAGAAAAAAATGGACACGCCCAGTTCGGACAAGACGATATTGTCATC
GAGTCCGAACACACCTCACG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: SEH; Cytosolic epoxide hydrolase; cEH; Epoxide hydratase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MIEIRHKTVHANGIRQHYLEVGNGPPIVLLHGFPETSYAWRHQIPVLAEHYRVIAPDLRGYGETDKPAAGYDKRTMALDI
VALLKALDIPKIALIGHDRGARVATRFAKDHPALLDRLVVMDNVPTRIVARSVNAQVAKAYWFFFFHLVPDLPEALIAGR
EDLWLRHFFSDWCYNPHTISGEAFDTYVQAYRRPGAVRGAMADYRANLEDNAQDQIDADVKITCPVLSLWGEDFEAVGKM
FDMPSIWAEMAHNLRAEPIAQCGHLPHEEQPERVNKLLLEFLDGWAG

Sequences:

>Translated_287_residues
MIEIRHKTVHANGIRQHYLEVGNGPPIVLLHGFPETSYAWRHQIPVLAEHYRVIAPDLRGYGETDKPAAGYDKRTMALDI
VALLKALDIPKIALIGHDRGARVATRFAKDHPALLDRLVVMDNVPTRIVARSVNAQVAKAYWFFFFHLVPDLPEALIAGR
EDLWLRHFFSDWCYNPHTISGEAFDTYVQAYRRPGAVRGAMADYRANLEDNAQDQIDADVKITCPVLSLWGEDFEAVGKM
FDMPSIWAEMAHNLRAEPIAQCGHLPHEEQPERVNKLLLEFLDGWAG
>Mature_287_residues
MIEIRHKTVHANGIRQHYLEVGNGPPIVLLHGFPETSYAWRHQIPVLAEHYRVIAPDLRGYGETDKPAAGYDKRTMALDI
VALLKALDIPKIALIGHDRGARVATRFAKDHPALLDRLVVMDNVPTRIVARSVNAQVAKAYWFFFFHLVPDLPEALIAGR
EDLWLRHFFSDWCYNPHTISGEAFDTYVQAYRRPGAVRGAMADYRANLEDNAQDQIDADVKITCPVLSLWGEDFEAVGKM
FDMPSIWAEMAHNLRAEPIAQCGHLPHEEQPERVNKLLLEFLDGWAG

Specific function: Involved in catabolic degradation of epoxides. Shows highest activity towards C6 and C7 carbocyclic epoxides. Also active towards linear 1,2-epoxyalkanes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=288, Percent_Identity=31.9444444444444, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI218777840, Length=291, Percent_Identity=28.1786941580756, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI13376164, Length=291, Percent_Identity=28.1786941580756, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI27597073, Length=304, Percent_Identity=28.2894736842105, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17562298, Length=289, Percent_Identity=28.0276816608997, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17554012, Length=294, Percent_Identity=26.8707482993197, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324392, Length=120, Percent_Identity=35.8333333333333, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR010497 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF06441 EHN [H]

EC number: =3.3.2.10 [H]

Molecular weight: Translated: 32523; Mature: 32523

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIRHKTVHANGIRQHYLEVGNGPPIVLLHGFPETSYAWRHQIPVLAEHYRVIAPDLRG
CEEEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCHHHHHHHEECCHHCC
YGETDKPAAGYDKRTMALDIVALLKALDIPKIALIGHDRGARVATRFAKDHPALLDRLVV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHH
MDNVPTRIVARSVNAQVAKAYWFFFFHLVPDLPEALIAGREDLWLRHFFSDWCYNPHTIS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
GEAFDTYVQAYRRPGAVRGAMADYRANLEDNAQDQIDADVKITCPVLSLWGEDFEAVGKM
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHCCCCEEEECHHHHHCCCCHHHHHHH
FDMPSIWAEMAHNLRAEPIAQCGHLPHEEQPERVNKLLLEFLDGWAG
CCCHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEIRHKTVHANGIRQHYLEVGNGPPIVLLHGFPETSYAWRHQIPVLAEHYRVIAPDLRG
CEEEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCHHHHHHHEECCHHCC
YGETDKPAAGYDKRTMALDIVALLKALDIPKIALIGHDRGARVATRFAKDHPALLDRLVV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHH
MDNVPTRIVARSVNAQVAKAYWFFFFHLVPDLPEALIAGREDLWLRHFFSDWCYNPHTIS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
GEAFDTYVQAYRRPGAVRGAMADYRANLEDNAQDQIDADVKITCPVLSLWGEDFEAVGKM
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHCCCCEEEECHHHHHCCCCHHHHHHH
FDMPSIWAEMAHNLRAEPIAQCGHLPHEEQPERVNKLLLEFLDGWAG
CCCHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9578475 [H]