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The map label for this gene is ispB [H]

Identifier: 182677848

GI number: 182677848

Start: 973785

End: 974795

Strand: Direct

Name: ispB [H]

Synonym: Bind_0855

Alternate gene names: 182677848

Gene position: 973785-974795 (Clockwise)

Preceding gene: 182677847

Following gene: 182677849

Centisome position: 23.35

GC content: 58.95

Gene sequence:

>1011_bases
TTGGGTATTGTGATTCCTCTCGAAGACGCAAAAGAGTCCGGCATCGAAAGGCTCCTTGCCCTGGTGACGCCCGATATGGA
GCGCGTCAATCAGACCATTCTTTCGCGGGCTGCCTCCGATGTGACGATGATTCCGGAAGTCGCCAATCATCTGATTTCCT
CGGGCGGCAAGAGGCTGCGTCCCATGCTTACATTGGCGGCGGCCGGCCTCTGCGGCTATGGCGGCAATGGGCATATCAAG
CTCGCGGCCTCGGTCGAATTTATGCATACCGCGACCCTTCTGCATGACGATGTGGTCGATGAAAGCGATATGCGCCGGGG
CAAGCTCGCAGCCCGCATGCTCTGGGGCAATGAAGCCAGTGTCCTCGTTGGCGATTTCCTGCTCGGCCAGGCGTTCAAGA
TGATGGTTGAGGTGGGCTCGCTCGCCTGTCTCGAGGTTCTGTCCACGGCGGCAGCGGTAATCGCTGAGGGCGAGGTCATG
CAATTGGCGGCAGCCAAGGATACTGAGACGGGTGAGGATGATTATCTCGCCGTCATCCGCGCCAAGACCGCAGCCCTGTT
CGGCGCTGCCGCTGAGGTCGGCGCCCTTTTGGCGGAACGGCCAAAGGCGGAAATCGCCGCCTGTCGCAGTTACGGGCTCA
ATCTCGGCATTGCTTTTCAATTGATCGATGATGCGCTGGACTATGGGGGGACCTCCGCCAAGCTTGGCAAGAATGTCGGC
GACGATTTTCGAGAGGGTAAGATCACCTTGCCGGTCGTCCTCTGCTTCCGGCGAGGGACCCAGGACGAGCGCATTTTCTG
GCGTCGCACCCTCGAAAAGGGGGAAATTGAGGAAGGCGATCTCGATATAGCCATCGCTACAATGCGCAAGCATCGCGCCA
TCGAGGATACGGTGGAACGGGCCCGCCACTATGGCGCCATGGCGCGTGATGCTTTGGAGCTTTTTCCTGCCTCGCCCTGG
AAAATCGCCCTGAACGATGTCGTTGATTTCTGTGTCAACCGCATTTCGTAA

Upstream 100 bases:

>100_bases
CGGTTCTAGTTTCCTCGGCAGGATCGGCTATCTCGATCTCGGCTCTATGATTTTGCCGAGGGTCTGAGATCTTGAATAGG
GCCGCGATCGGAGATCTGAG

Downstream 100 bases:

>100_bases
ATCCTTATCTGTCTCGTTTTGAAACTCTCTCGGCAATTTCCTCTGTAACCGCTTCGCAAGCATAAATAGCGATAACCATT
TCCGGATGATGATGTGGCCT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MGIVIPLEDAKESGIERLLALVTPDMERVNQTILSRAASDVTMIPEVANHLISSGGKRLRPMLTLAAAGLCGYGGNGHIK
LAASVEFMHTATLLHDDVVDESDMRRGKLAARMLWGNEASVLVGDFLLGQAFKMMVEVGSLACLEVLSTAAAVIAEGEVM
QLAAAKDTETGEDDYLAVIRAKTAALFGAAAEVGALLAERPKAEIAACRSYGLNLGIAFQLIDDALDYGGTSAKLGKNVG
DDFREGKITLPVVLCFRRGTQDERIFWRRTLEKGEIEEGDLDIAIATMRKHRAIEDTVERARHYGAMARDALELFPASPW
KIALNDVVDFCVNRIS

Sequences:

>Translated_336_residues
MGIVIPLEDAKESGIERLLALVTPDMERVNQTILSRAASDVTMIPEVANHLISSGGKRLRPMLTLAAAGLCGYGGNGHIK
LAASVEFMHTATLLHDDVVDESDMRRGKLAARMLWGNEASVLVGDFLLGQAFKMMVEVGSLACLEVLSTAAAVIAEGEVM
QLAAAKDTETGEDDYLAVIRAKTAALFGAAAEVGALLAERPKAEIAACRSYGLNLGIAFQLIDDALDYGGTSAKLGKNVG
DDFREGKITLPVVLCFRRGTQDERIFWRRTLEKGEIEEGDLDIAIATMRKHRAIEDTVERARHYGAMARDALELFPASPW
KIALNDVVDFCVNRIS
>Mature_335_residues
GIVIPLEDAKESGIERLLALVTPDMERVNQTILSRAASDVTMIPEVANHLISSGGKRLRPMLTLAAAGLCGYGGNGHIKL
AASVEFMHTATLLHDDVVDESDMRRGKLAARMLWGNEASVLVGDFLLGQAFKMMVEVGSLACLEVLSTAAAVIAEGEVMQ
LAAAKDTETGEDDYLAVIRAKTAALFGAAAEVGALLAERPKAEIAACRSYGLNLGIAFQLIDDALDYGGTSAKLGKNVGD
DFREGKITLPVVLCFRRGTQDERIFWRRTLEKGEIEEGDLDIAIATMRKHRAIEDTVERARHYGAMARDALELFPASPWK
IALNDVVDFCVNRIS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=28.2828282828283, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=46.25, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1786623, Length=223, Percent_Identity=32.7354260089686, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=265, Percent_Identity=31.3207547169811, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=304, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24651612, Length=306, Percent_Identity=31.3725490196078, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36273; Mature: 36142

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIVIPLEDAKESGIERLLALVTPDMERVNQTILSRAASDVTMIPEVANHLISSGGKRLR
CCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
PMLTLAAAGLCGYGGNGHIKLAASVEFMHTATLLHDDVVDESDMRRGKLAARMLWGNEAS
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCH
VLVGDFLLGQAFKMMVEVGSLACLEVLSTAAAVIAEGEVMQLAAAKDTETGEDDYLAVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCEEEEHH
AKTAALFGAAAEVGALLAERPKAEIAACRSYGLNLGIAFQLIDDALDYGGTSAKLGKNVG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCCCC
DDFREGKITLPVVLCFRRGTQDERIFWRRTLEKGEIEEGDLDIAIATMRKHRAIEDTVER
CHHHCCCEEHHHHHEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
ARHYGAMARDALELFPASPWKIALNDVVDFCVNRIS
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
GIVIPLEDAKESGIERLLALVTPDMERVNQTILSRAASDVTMIPEVANHLISSGGKRLR
CEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
PMLTLAAAGLCGYGGNGHIKLAASVEFMHTATLLHDDVVDESDMRRGKLAARMLWGNEAS
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCH
VLVGDFLLGQAFKMMVEVGSLACLEVLSTAAAVIAEGEVMQLAAAKDTETGEDDYLAVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCEEEEHH
AKTAALFGAAAEVGALLAERPKAEIAACRSYGLNLGIAFQLIDDALDYGGTSAKLGKNVG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCCCC
DDFREGKITLPVVLCFRRGTQDERIFWRRTLEKGEIEEGDLDIAIATMRKHRAIEDTVER
CHHHCCCEEHHHHHEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
ARHYGAMARDALELFPASPWKIALNDVVDFCVNRIS
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]