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The map label for this gene is ligA

Identifier: 182677699

GI number: 182677699

Start: 791563

End: 793662

Strand: Direct

Name: ligA

Synonym: Bind_0706

Alternate gene names: 182677699

Gene position: 791563-793662 (Clockwise)

Preceding gene: 182677698

Following gene: 182677700

Centisome position: 18.98

GC content: 59.52

Gene sequence:

>2100_bases
ATGACCGTGCAAAAACCCATCGAGAGCCTCAGCCCTGCCCAGGCGAAGCGCGAACATAGACGTCTCGGTGAACAGATCAC
CGAGCACGATCGGCTTTATTATCAGGAAGATGCGCCGAGGGTCTCCGACGCCGAATATGATGCCTTGCGCCGCCGCTACG
AGGCGCTCGAGACGGCTTTTCCGGAGCTTGTCAGCGCTGAATCCCTGACCAAGAAGATTGGCGCGGCACCGGCCGAGAAA
TTCGCCAAAATCATTCATAAAGTGCCCATGCTGTCGCTGGCCAATATTTTCAGCGATGAGGAAGTCGGTGAATTCGTTGC
GCGTGTGCGTCGTTTTCTCGGGCTTGGCGCTGACGCGCCGCTGGCCATCAGTGCCGAGCCGAAGATCGACGGTCTGTCCT
GTTCCTTGCGCTATGAAGGCGGCTATCTCGTCCAGGCGGCGACGCGCGGCGACGGCTATGAGGGCGAGGATGTGACCGCC
AATGTGCGGACGATCCAAGAAATCCCGCACCAGCTCCAGGGCCATGCGCCCGAAATTCTGGAGGTTCGCGGCGAGGTCTA
TATGACCCATGCCGATTTCGCCGCGCTCAACGAGAGACAGGAAGCGGCGGGCAAAACAATCTTTGCCAATCCACGCAATT
CGGCGGCGGGTTCCTTGCGCCAGCTCGATCCCAAGGTCACCGCGGGGCGCCCCTTGCATTTCTTCGCCTATGCCTGGGGC
GAGGCCAGTGAGGAACCAGCCGACACACAAATGGGCATGGTCACGGCCTTCAAGGCCTTTGGCCTGCCGGTCAACCCGCT
CATGCAGCTTTGCCATTCGGCGGAGGACTTGATCGCCCATTATCATGACATCGAGGCACGGCGGGCCTTGCTCGATTATG
ATATCGATGGCGTGGTCTACAAGGTCAATTCCATTGCCTTGCAGAAGCGTCTTGGATTTGTGTCGCGCGCGCCGCGCTGG
GCGACTGCGCATAAATTTCCGGCCGAAAAGGCTGTGACGCTGTTACGCGATATTGAGATCCAGGTTGGCCGCACGGGCGC
GCTGACGCCGGTCGCTCGGCTTGAGCCGATTACCGTCGGCGGTGTTGTCGTTTCCAATGCGACCTTGCACAATGAGGATG
AGATTGCGCGCAAGGACATACGCATCGGCGATATGGTGATGATCCAGCGTGCCGGCGATGTCATTCCGCAAATTTTGGGT
CCGATCCTCGATAAACGCCCCCAGGATGCGCAACCCTATGAATTTCCGCAGGTCTGTCCGGTTTGTGGCTCAGCGGCGAT
CCGTGAGATCGATCCGAAAACCGGGACCGCCGATGTCGTGCGCCGTTGCACGGGCGGGCTCGTCTGCGCCGCGCAGGTCG
TGGAGCGCCTCAAACATTTCGCGTCCCGCAATGCGTTCGATATCGAGGGTTTGGGGGACAAGCAGATCGAGCAATTCTAT
CATGATGGGCTGATCCATACGCCCGTCGATATTTTCACTTTGCAAGAGCGTGATGCGCGAAGCCTGAAGAAATTGAAGGA
TCGCGAGGGCTATGGAGAAACCTCGGTGCGCAATCTGTTTCAGGCGATCGAGGCGCGCCGGCATATTCCCGTCAACCGTT
TCATTTATGCGCTTGGCATCAGGCATGTGGGCGAGACCAATGCCCGCCGCGCGGCGCGCGCCTTTGGCACATTCGACGCC
TTGCGCGCAGTCGCAAGCCGGGCAGAGGAGGGCTCGGAGGAACGGTCCGAACTCACCAATGTCGAGGGTTTCGGGCCTGT
GGTTGCCGAGGCCATTTTCGATTTCTTCCATGAGCAACACAATCAAGACGTGCTCGATGGTCTCTTGGAGCAGGTGACGC
CGGAGCCTATGGAGGCCGTGGCGAAAGAAAGTCCCGTGGCGGGCAAGACGGTCGTCTTCACCGGCGCGCTCGAACATATG
ACGCGCGAGGAAGCCAAGGCCCAAGCCGAACGGCTTGGAGCCAAGGTCGCGGGCTCTGTCTCGAAAAAGACCGATCTCGT
CGTCGCGGGTCCAGGAGCAGGAAGCAAGCTTGCCAAGGCGGCGGAACTGAACATCGAGACGATTAGCGAGGAAGACTGGC
TGAAGCTTGTCGGGGAATAA

Upstream 100 bases:

>100_bases
GCCAAAGTTTATGAGTCGACGCCCTAGTGCACCGGGAACAAGCAAATAGCCTTCAAGAGTCCTCTTTTCGCCTCTGTAGC
GGCCATGCTAATCATGCGGC

Downstream 100 bases:

>100_bases
GCCCTTCCTGTCATTCGGTGCGGACAGGAACCAGAAAGCTGGACGTCAGTTGCCCATCGAGTGCGGAAGGATTGGGCCTA
TGAGGAAGAGGCGACGATTT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MTVQKPIESLSPAQAKREHRRLGEQITEHDRLYYQEDAPRVSDAEYDALRRRYEALETAFPELVSAESLTKKIGAAPAEK
FAKIIHKVPMLSLANIFSDEEVGEFVARVRRFLGLGADAPLAISAEPKIDGLSCSLRYEGGYLVQAATRGDGYEGEDVTA
NVRTIQEIPHQLQGHAPEILEVRGEVYMTHADFAALNERQEAAGKTIFANPRNSAAGSLRQLDPKVTAGRPLHFFAYAWG
EASEEPADTQMGMVTAFKAFGLPVNPLMQLCHSAEDLIAHYHDIEARRALLDYDIDGVVYKVNSIALQKRLGFVSRAPRW
ATAHKFPAEKAVTLLRDIEIQVGRTGALTPVARLEPITVGGVVVSNATLHNEDEIARKDIRIGDMVMIQRAGDVIPQILG
PILDKRPQDAQPYEFPQVCPVCGSAAIREIDPKTGTADVVRRCTGGLVCAAQVVERLKHFASRNAFDIEGLGDKQIEQFY
HDGLIHTPVDIFTLQERDARSLKKLKDREGYGETSVRNLFQAIEARRHIPVNRFIYALGIRHVGETNARRAARAFGTFDA
LRAVASRAEEGSEERSELTNVEGFGPVVAEAIFDFFHEQHNQDVLDGLLEQVTPEPMEAVAKESPVAGKTVVFTGALEHM
TREEAKAQAERLGAKVAGSVSKKTDLVVAGPGAGSKLAKAAELNIETISEEDWLKLVGE

Sequences:

>Translated_699_residues
MTVQKPIESLSPAQAKREHRRLGEQITEHDRLYYQEDAPRVSDAEYDALRRRYEALETAFPELVSAESLTKKIGAAPAEK
FAKIIHKVPMLSLANIFSDEEVGEFVARVRRFLGLGADAPLAISAEPKIDGLSCSLRYEGGYLVQAATRGDGYEGEDVTA
NVRTIQEIPHQLQGHAPEILEVRGEVYMTHADFAALNERQEAAGKTIFANPRNSAAGSLRQLDPKVTAGRPLHFFAYAWG
EASEEPADTQMGMVTAFKAFGLPVNPLMQLCHSAEDLIAHYHDIEARRALLDYDIDGVVYKVNSIALQKRLGFVSRAPRW
ATAHKFPAEKAVTLLRDIEIQVGRTGALTPVARLEPITVGGVVVSNATLHNEDEIARKDIRIGDMVMIQRAGDVIPQILG
PILDKRPQDAQPYEFPQVCPVCGSAAIREIDPKTGTADVVRRCTGGLVCAAQVVERLKHFASRNAFDIEGLGDKQIEQFY
HDGLIHTPVDIFTLQERDARSLKKLKDREGYGETSVRNLFQAIEARRHIPVNRFIYALGIRHVGETNARRAARAFGTFDA
LRAVASRAEEGSEERSELTNVEGFGPVVAEAIFDFFHEQHNQDVLDGLLEQVTPEPMEAVAKESPVAGKTVVFTGALEHM
TREEAKAQAERLGAKVAGSVSKKTDLVVAGPGAGSKLAKAAELNIETISEEDWLKLVGE
>Mature_698_residues
TVQKPIESLSPAQAKREHRRLGEQITEHDRLYYQEDAPRVSDAEYDALRRRYEALETAFPELVSAESLTKKIGAAPAEKF
AKIIHKVPMLSLANIFSDEEVGEFVARVRRFLGLGADAPLAISAEPKIDGLSCSLRYEGGYLVQAATRGDGYEGEDVTAN
VRTIQEIPHQLQGHAPEILEVRGEVYMTHADFAALNERQEAAGKTIFANPRNSAAGSLRQLDPKVTAGRPLHFFAYAWGE
ASEEPADTQMGMVTAFKAFGLPVNPLMQLCHSAEDLIAHYHDIEARRALLDYDIDGVVYKVNSIALQKRLGFVSRAPRWA
TAHKFPAEKAVTLLRDIEIQVGRTGALTPVARLEPITVGGVVVSNATLHNEDEIARKDIRIGDMVMIQRAGDVIPQILGP
ILDKRPQDAQPYEFPQVCPVCGSAAIREIDPKTGTADVVRRCTGGLVCAAQVVERLKHFASRNAFDIEGLGDKQIEQFYH
DGLIHTPVDIFTLQERDARSLKKLKDREGYGETSVRNLFQAIEARRHIPVNRFIYALGIRHVGETNARRAARAFGTFDAL
RAVASRAEEGSEERSELTNVEGFGPVVAEAIFDFFHEQHNQDVLDGLLEQVTPEPMEAVAKESPVAGKTVVFTGALEHMT
REEAKAQAERLGAKVAGSVSKKTDLVVAGPGAGSKLAKAAELNIETISEEDWLKLVGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=689, Percent_Identity=50.9433962264151, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=540, Percent_Identity=22.2222222222222, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BEII9 (B2IGH4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831845.1
- ProteinModelPortal:   B2IGH4
- GeneID:   6200171
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0706
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK979523
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76840; Mature: 76709

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2; PS00165 DEHYDRATASE_SER_THR

Important sites: ACT_SITE 128-128 BINDING 126-126 BINDING 149-149 BINDING 185-185 BINDING 301-301 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQKPIESLSPAQAKREHRRLGEQITEHDRLYYQEDAPRVSDAEYDALRRRYEALETAF
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
PELVSAESLTKKIGAAPAEKFAKIIHKVPMLSLANIFSDEEVGEFVARVRRFLGLGADAP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
LAISAEPKIDGLSCSLRYEGGYLVQAATRGDGYEGEDVTANVRTIQEIPHQLQGHAPEIL
EEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
EVRGEVYMTHADFAALNERQEAAGKTIFANPRNSAAGSLRQLDPKVTAGRPLHFFAYAWG
HHCCCEEEEEHHHHHHHHHHHHCCCEEEECCCCCHHCHHHHCCCCCCCCCCEEEEEEECC
EASEEPADTQMGMVTAFKAFGLPVNPLMQLCHSAEDLIAHYHDIEARRALLDYDIDGVVY
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KVNSIALQKRLGFVSRAPRWATAHKFPAEKAVTLLRDIEIQVGRTGALTPVARLEPITVG
EHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHEECCCCCCCCHHHCCCEEEC
GVVVSNATLHNEDEIARKDIRIGDMVMIQRAGDVIPQILGPILDKRPQDAQPYEFPQVCP
CEEEECCEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
VCGSAAIREIDPKTGTADVVRRCTGGLVCAAQVVERLKHFASRNAFDIEGLGDKQIEQFY
HHCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HDGLIHTPVDIFTLQERDARSLKKLKDREGYGETSVRNLFQAIEARRHIPVNRFIYALGI
HCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RHVGETNARRAARAFGTFDALRAVASRAEEGSEERSELTNVEGFGPVVAEAIFDFFHEQH
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHC
NQDVLDGLLEQVTPEPMEAVAKESPVAGKTVVFTGALEHMTREEAKAQAERLGAKVAGSV
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
SKKTDLVVAGPGAGSKLAKAAELNIETISEEDWLKLVGE
CCCCCEEEECCCCCHHHHHHHHCCHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TVQKPIESLSPAQAKREHRRLGEQITEHDRLYYQEDAPRVSDAEYDALRRRYEALETAF
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
PELVSAESLTKKIGAAPAEKFAKIIHKVPMLSLANIFSDEEVGEFVARVRRFLGLGADAP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
LAISAEPKIDGLSCSLRYEGGYLVQAATRGDGYEGEDVTANVRTIQEIPHQLQGHAPEIL
EEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
EVRGEVYMTHADFAALNERQEAAGKTIFANPRNSAAGSLRQLDPKVTAGRPLHFFAYAWG
HHCCCEEEEEHHHHHHHHHHHHCCCEEEECCCCCHHCHHHHCCCCCCCCCCEEEEEEECC
EASEEPADTQMGMVTAFKAFGLPVNPLMQLCHSAEDLIAHYHDIEARRALLDYDIDGVVY
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KVNSIALQKRLGFVSRAPRWATAHKFPAEKAVTLLRDIEIQVGRTGALTPVARLEPITVG
EHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHEECCCCCCCCHHHCCCEEEC
GVVVSNATLHNEDEIARKDIRIGDMVMIQRAGDVIPQILGPILDKRPQDAQPYEFPQVCP
CEEEECCEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
VCGSAAIREIDPKTGTADVVRRCTGGLVCAAQVVERLKHFASRNAFDIEGLGDKQIEQFY
HHCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HDGLIHTPVDIFTLQERDARSLKKLKDREGYGETSVRNLFQAIEARRHIPVNRFIYALGI
HCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RHVGETNARRAARAFGTFDALRAVASRAEEGSEERSELTNVEGFGPVVAEAIFDFFHEQH
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHC
NQDVLDGLLEQVTPEPMEAVAKESPVAGKTVVFTGALEHMTREEAKAQAERLGAKVAGSV
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
SKKTDLVVAGPGAGSKLAKAAELNIETISEEDWLKLVGE
CCCCCEEEECCCCCHHHHHHHHCCHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA