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The map label for this gene is pyk [H]

Identifier: 182677623

GI number: 182677623

Start: 711960

End: 713393

Strand: Direct

Name: pyk [H]

Synonym: Bind_0630

Alternate gene names: 182677623

Gene position: 711960-713393 (Clockwise)

Preceding gene: 182677622

Following gene: 182677624

Centisome position: 17.07

GC content: 65.97

Gene sequence:

>1434_bases
ATGCGCCGGTATCGCCGAGCCAAGATTGTGGCAACACTGGGTCCCGCGAGTTCCAGTCCGGAAACCATCCGGGCTCTGTT
TGACGCAGGCGCCGATGTGTTTCGGTTCAATTTCAGCCATGGCAGCCATGAGGACCATCGGCAGCGCTATGAGATCGTGC
GCGCCATCGAGCAGGAAACCGGCCGGCCGATCTCGGTCCTCGCCGATCTGCAAGGGCCGAAACTGCGCATCGGCAAGCTG
GCCGCGGGGCCGGTCATGCTGGAGGAAGGCACGACGATCACCTTTGATCTCGATCCGGAGCCCGGCACGCTCGCGCGGGT
GCCTCTGCCGCATCCGGAAGTCTTCGCGGCGCTTTCGCCTGGGGTCACGCTGCTGATCGATGATGGCAAGCTGCGCCTCA
CCATCGAGAAGGCCGAGCCGCAATCGGCCGTGGCGAAGGTGGTGACCGGGGGCCCGCTTTCCGAGCGCAAGGGCGTCAGC
ATCGTCGGGGCGGTTCTGCCGGTCTCGGCGCTGACCGAAAAGGATCGCAAGGATCTCGCTTTCGCGCTGGAGCTTGGCGC
CGACTGGATCGCTTTGTCCTTCGTGCAGCGGGCCGAAGATCTCGACGAGGTGCGCGCGCTGGCCAATCGTCCGATCGCGC
TGATGGCGAAGCTCGAAAAGCCTTCCGCCATCGACGGGCTCGAAGCCATTATCGCACGATCCGATGCGGTCATGGTGGCG
CGCGGCGATCTTGGTGTTGAAATGGAGCCCGAGCGTGTGCCGATCGTGCAGCGGCGCATCGTGCATGCGGCGCGGGGGGC
CGGCAAGCCGGTGATCGTTGCCACGCAAATGCTGGAATCGATGATCACCACGCCGACGCCGACCCGCGCCGAGGCCTCGG
ATGTCGCGACCGCCATCTATGAGGGCGCCGATGCGGTGATGCTGTCCGCGGAGACGGCGTCTGGCCGCTATCCGATCGAG
GCTGTGACGATGATGAACAAGATCATCACGGCCGTGGAGCAGGACCCTGTATATCGTGAAGGGCTTGATGCGACGGGGCC
GACGCCGCAGGCGAATGTCTCGGATGTGATCTGCAGCGCCTTGCATCGCTCGGCGGCGATCCTGCCTGTGGCGGCGCTGG
TCACCTATACGACCTCGGGCCTGACGAGCCTGCGCACGGCGCGGGAACGGCCGGCGGCGCCGATCCTGAGCCTGACGCCG
GAATTGGCGATCGCGCGGCGCCTGGCGCTGGTTTGGGGCACGCATCCGGTGCTCAGCCGTGCGCAGGCGCATGTGACGGA
CATCGTCGAGGATGCCTGCGCCGCGACGGAAAAGGAAGGGCTGGCGATCCCCGGCGATATTATCGCCATAGCCGGCGGCA
TGCCCTTTGGCGTGTCCGGCACGACCAATCTCCTGCGCATCGCACAAGTGCCAGGCGGTGCCGCCGCCAAATAG

Upstream 100 bases:

>100_bases
TCGAGAAAGCGAGCGCGGCAGCTGCCGCCATACTCATACGTTGACCTTATACAAACAATATTTTCACCTAGGGAGGCGGC
CCGCTGGGCCGATTGAGATC

Downstream 100 bases:

>100_bases
GATCGCTTTAGATCCCTGCCTTGAATGGATCCACATCAACCCCAAAAGCTCCCGCTTTTGGGGTTTTTGCTTGTGAAGGT
GGGGAAATTTCGGCTGGTTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGSHEDHRQRYEIVRAIEQETGRPISVLADLQGPKLRIGKL
AAGPVMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSPGVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVS
IVGAVLPVSALTEKDRKDLAFALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIYEGADAVMLSAETASGRYPIE
AVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSALHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTP
ELAIARRLALVWGTHPVLSRAQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPGGAAAK

Sequences:

>Translated_477_residues
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGSHEDHRQRYEIVRAIEQETGRPISVLADLQGPKLRIGKL
AAGPVMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSPGVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVS
IVGAVLPVSALTEKDRKDLAFALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIYEGADAVMLSAETASGRYPIE
AVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSALHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTP
ELAIARRLALVWGTHPVLSRAQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPGGAAAK
>Mature_477_residues
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGSHEDHRQRYEIVRAIEQETGRPISVLADLQGPKLRIGKL
AAGPVMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSPGVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVS
IVGAVLPVSALTEKDRKDLAFALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIYEGADAVMLSAETASGRYPIE
AVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSALHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTP
ELAIARRLALVWGTHPVLSRAQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPGGAAAK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=36.1224489795918, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=36.1224489795918, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=34.8979591836735, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=34.8979591836735, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=35.1626016260163, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=33.4494773519164, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=33.4494773519164, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=31.063829787234, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=31.063829787234, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=29.4392523364486, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788160, Length=488, Percent_Identity=39.344262295082, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=37.3931623931624, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=37.1369294605809, Blast_Score=280, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=37.1369294605809, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=37.1369294605809, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=37.1369294605809, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=34.0163934426229, Blast_Score=278, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=32.2314049586777, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=32.9938900203666, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=33.739837398374, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=33.739837398374, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=35.9605911330049, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=30.6910569105691, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24646914, Length=287, Percent_Identity=36.2369337979094, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50474; Mature: 50474

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGSHEDHRQRYEIVRAIEQET
CCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPISVLADLQGPKLRIGKLAAGPVMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSP
CCCEEEEECCCCCCEEECEEECCCEEEECCCEEEEEECCCCCCEEECCCCCHHHHHCCCC
GVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVSIVGAVLPVSALTEKDRKDLA
CEEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCHHHHE
FALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
EEEHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEE
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIY
ECCCCCEECCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EGADAVMLSAETASGRYPIEAVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHH
LHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTPELAIARRLALVWGTHPVLSR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHH
AQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPGGAAAK
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGSHEDHRQRYEIVRAIEQET
CCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPISVLADLQGPKLRIGKLAAGPVMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSP
CCCEEEEECCCCCCEEECEEECCCEEEECCCEEEEEECCCCCCEEECCCCCHHHHHCCCC
GVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVSIVGAVLPVSALTEKDRKDLA
CEEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCHHHHE
FALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
EEEHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEE
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIY
ECCCCCEECCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EGADAVMLSAETASGRYPIEAVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHH
LHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTPELAIARRLALVWGTHPVLSR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHH
AQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPGGAAAK
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA