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The map label for this gene is eda [H]

Identifier: 182677622

GI number: 182677622

Start: 711247

End: 711903

Strand: Direct

Name: eda [H]

Synonym: Bind_0629

Alternate gene names: 182677622

Gene position: 711247-711903 (Clockwise)

Preceding gene: 182677621

Following gene: 182677623

Centisome position: 17.06

GC content: 63.32

Gene sequence:

>657_bases
ATGAGCCATGATCCTCACACAGACTTGCAATCCGGCTTAGCCTCGATCCTGACCTCGGCGCCGGTCGTTCCCGTCGTCAC
GATCGAGGATCTGGCCGATGCCGTGCCGCTCGCCAAGGCGCTCGTCGCCGGCGGTCTTCCGGCCATTGAAGTGACCTTGC
GCACCAAGGTTGCGATGGAGGCCATTCGTGTCATCGCCGCCGAGGTCGAGGGCGCGATCCCCGGCGTCGGCACGATTTTG
GAGGCAAGCCAACTCGAGGCGGCGCAAAAGGCGGGGGCGCGTTTCGCCGTTTCACCCGGGGCGACGGACCGCCTGCTTGA
TGCCTCAGCCGCACTCAGCCTGCCGCTGCTGCCGGGCATTGCAACAGCCTCCGAAGCCATGGCCTTGATTGAGCGCGGCT
TCACTTATGCCAAATTCTTCCCGGCGGAGCCCGCGGGCGGCATCCCCTATCTTTCGGCTCTGGCCACGCCTTTACCGCAA
CTGACTTTCTGCCCTACGGGTGGCGTGTCAATCGATAGCGCACCGCGCTATCTGGCGCTGCCCAATGTCATCTGCGTCGG
CGGTTCCTGGATGCTCAAACGCGATCATATCAAGGCCAAGGATTGGACCGCTATCGAGAAAGCGAGCGCGGCAGCTGCCG
CCATACTCATACGTTGA

Upstream 100 bases:

>100_bases
GGCCTGCCTGCCTTCAAGCCGAGCGTTAATACATATGAAACGCGTATTCGTGTTCAGCGCGCGTTCTAAAATTTGTGATA
GTCCGGTTCGGAGTTCCGGC

Downstream 100 bases:

>100_bases
CCTTATACAAACAATATTTTCACCTAGGGAGGCGGCCCGCTGGGCCGATTGAGATCATGCGCCGGTATCGCCGAGCCAAG
ATTGTGGCAACACTGGGTCC

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSHDPHTDLQSGLASILTSAPVVPVVTIEDLADAVPLAKALVAGGLPAIEVTLRTKVAMEAIRVIAAEVEGAIPGVGTIL
EASQLEAAQKAGARFAVSPGATDRLLDASAALSLPLLPGIATASEAMALIERGFTYAKFFPAEPAGGIPYLSALATPLPQ
LTFCPTGGVSIDSAPRYLALPNVICVGGSWMLKRDHIKAKDWTAIEKASAAAAAILIR

Sequences:

>Translated_218_residues
MSHDPHTDLQSGLASILTSAPVVPVVTIEDLADAVPLAKALVAGGLPAIEVTLRTKVAMEAIRVIAAEVEGAIPGVGTIL
EASQLEAAQKAGARFAVSPGATDRLLDASAALSLPLLPGIATASEAMALIERGFTYAKFFPAEPAGGIPYLSALATPLPQ
LTFCPTGGVSIDSAPRYLALPNVICVGGSWMLKRDHIKAKDWTAIEKASAAAAAILIR
>Mature_217_residues
SHDPHTDLQSGLASILTSAPVVPVVTIEDLADAVPLAKALVAGGLPAIEVTLRTKVAMEAIRVIAAEVEGAIPGVGTILE
ASQLEAAQKAGARFAVSPGATDRLLDASAALSLPLLPGIATASEAMALIERGFTYAKFFPAEPAGGIPYLSALATPLPQL
TFCPTGGVSIDSAPRYLALPNVICVGGSWMLKRDHIKAKDWTAIEKASAAAAAILIR

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=200, Percent_Identity=50, Blast_Score=177, Evalue=5e-46,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22325; Mature: 22194

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDPHTDLQSGLASILTSAPVVPVVTIEDLADAVPLAKALVAGGLPAIEVTLRTKVAME
CCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
AIRVIAAEVEGAIPGVGTILEASQLEAAQKAGARFAVSPGATDRLLDASAALSLPLLPGI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCH
ATASEAMALIERGFTYAKFFPAEPAGGIPYLSALATPLPQLTFCPTGGVSIDSAPRYLAL
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEC
PNVICVGGSWMLKRDHIKAKDWTAIEKASAAAAAILIR
CCEEEECCCEEEECCCCCCCCCHHHHHHHHHHEEEEEC
>Mature Secondary Structure 
SHDPHTDLQSGLASILTSAPVVPVVTIEDLADAVPLAKALVAGGLPAIEVTLRTKVAME
CCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
AIRVIAAEVEGAIPGVGTILEASQLEAAQKAGARFAVSPGATDRLLDASAALSLPLLPGI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCH
ATASEAMALIERGFTYAKFFPAEPAGGIPYLSALATPLPQLTFCPTGGVSIDSAPRYLAL
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEC
PNVICVGGSWMLKRDHIKAKDWTAIEKASAAAAAILIR
CCEEEECCCEEEECCCCCCCCCHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]