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The map label for this gene is metG

Identifier: 182682199

GI number: 182682199

Start: 1857470

End: 1859578

Strand: Direct

Name: metG

Synonym: XfasM23_1678

Alternate gene names: 182682199

Gene position: 1857470-1859578 (Clockwise)

Preceding gene: 182682198

Following gene: 182682201

Centisome position: 73.25

GC content: 57.14

Gene sequence:

>2109_bases
ATGCGATACTCGCACCCCCTGGTACCACTCTCCTTCATGACCACAGCACTTGTCACCACCGCCCTGCCTTACGCCAATGG
TCCACTGCACCTCGGCCATCTGGTCGGCTACATTCAAGCTGACATCTGGGTACGCGCACGGCGCCTGGGAGGACACAACA
CCTGGTTTGTTTGCGCCGACGACACCCACGGCACCCCGATCATGCTGGCCGCAGAAAAAGCGGGCATGCCGCCGGAAGCC
TTTATCGCCACCACCCAAGCCAGCCATGAACGCGACTTTGCGGCCTTCAACGTCGCTTTTGACCACTACGACTCCACACA
TTCCCCCGTCAACCGCCACCTGACCGAACAGTCTTACCTCACACTCAAACAGGCCGGACATATCACATGCCGCTCGGTCG
CCCAATTTTACGACCCGGCCAAAGGCATGTTCCTCCCCGACCGCTACGTCAAAGGAACCTGTCCAAACTGCGGCGCCACC
GACCAATATGGGGACAACTGCGAAGCATGCGGTGCCACCTATGACCCCACAGAACTCAAAAACCCATACTCGGTGATCTC
CGGCGCGACCCCAGAACTGCGCGACTCAGAACACTTCTTTTTTGAAGTGGCTCACTTCGACACCTTCCTACGCCATTGGC
TGAGCGGCGATGTCGCCTTGCCTAGCGTCAAAAACAAACTCAAAGAATGGCTGGATGCCAAAGGCGGCCTACGCCCCTGG
GACATCTCCCGCGATGCCCCCTACTTCGGCTTCAAAATCCCCGACCAACCAGGCAAGTACTTCTACGTGTGGCTGGATGC
ACCGATCGGCTACTTATGCAGCTTCAAAACCTTATGCACACGCATTGGCGAAGACTTCGACACACACCTACGCAGCGGCA
CCACGACAGAATTGCATCACTTTATCGGGAAAGACATCGTCAACTTCCACGCCCTGTTTTGGCCGGCAGTCCTGCACGGC
ACCGGCCACCGCGCCCCCACCCGACTCCATGTCAACGGCTACCTGACTGTGGACGGTGCCAAAATGTCCAAATCGCGCGG
CACCTTCATCATGGCGCGCACCTACCTTGATGCTGGCTTGGAACCAGACGCACTACGCTACTACTTCGCCGCCAAATCCT
CCGGAGACGTCGACGACCTGGACCTTAACCTGAGTGACTTCGTTGCACGCGTCAACGCCGACCTAGTCGGTAAACTCGTC
AACCTAGCCAGCCGCTGCGCAAGTTTCATTGGCACGCGTTTTAACGGACAACTCGCCGACACCCTGCCAGACCGCATCCA
ATATGATCGATTCGTTGCAGCACTGACCCCCATCCGTGACGCCTACGAACGCAACGACACCGCCAGCGCGATCCGTCAAA
CCATGCAGCTGGCTGACGAAGCCAACAAATACATTGACGAGACCAAACCCTGGATCATCGCCAAACAACACCACGCCGAC
GCGCAGCTACACGCCGTCTGCACACAAGGATTGAACCTGTTTCGCGTCCTGATCACCGCACTCAAACCAATCCTGCCGCA
CACCAGCATCCAAGCGGAGACATTCCTCGCTGCACCAGTAACGGCATGGCAAGACGTCAACCAACCACTGACCGGCGGCC
ACACCATTCAACCCTACTCCCCCTTATTCACCCGTATCGACAAAAAAATAATTGAGGTGATGATCAACGCCTCTAAAGAC
ACCCTCGCCCCTCCCCCTGCTTCGGCCAAACAACAAAACGCATCCATGTCAAACACAGCACCACCACCCACTGCGGAAGA
ACCGGAAACAACTGCTCCCACCATTGGTATTGACGACTTCGCCAAACTCGATCTACGCATCGGCAAAGTACTCGTGTGCG
AATACGTTGAAGGGTCAGACAAACTACTGCGCTTTGAATTGGATGCTGGCCCCCTGGGCAAGCGCCAGATCTTCTCAGGA
ATACGCGCCAGCTACAGCAACCCAGAGGCACTCATTGGCCGCAACGTCGTGTTCATCGCCAACCTAGCACCCCGCAAAAT
GCGCTTTGGGATCAGCCAAGGGATGATCCTCTCAGCCGGCTTCGACAACGGTACGCTGGCACTACTAGATGCTGACAGCA
GCGCCCAACCCGGCATGCCGGTGCGTTAA

Upstream 100 bases:

>100_bases
TGTTTTTTGGGCAAGCACGATGCAACACACTGCAATGACATAACACTGCAATGACATACCCTGATACACCGCTACGAGAG
ACCTGAATCAATCACCGGAC

Downstream 100 bases:

>100_bases
AAAAGCCCAGCCCCCAACACGCCACACACCAAAATGATCTCACTGGAGATGCCTACGAGAATGTCCTGTCATCTCCCGCG
TAAAACACGTGATACCGCCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MRYSHPLVPLSFMTTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLGGHNTWFVCADDTHGTPIMLAAEKAGMPPEA
FIATTQASHERDFAAFNVAFDHYDSTHSPVNRHLTEQSYLTLKQAGHITCRSVAQFYDPAKGMFLPDRYVKGTCPNCGAT
DQYGDNCEACGATYDPTELKNPYSVISGATPELRDSEHFFFEVAHFDTFLRHWLSGDVALPSVKNKLKEWLDAKGGLRPW
DISRDAPYFGFKIPDQPGKYFYVWLDAPIGYLCSFKTLCTRIGEDFDTHLRSGTTTELHHFIGKDIVNFHALFWPAVLHG
TGHRAPTRLHVNGYLTVDGAKMSKSRGTFIMARTYLDAGLEPDALRYYFAAKSSGDVDDLDLNLSDFVARVNADLVGKLV
NLASRCASFIGTRFNGQLADTLPDRIQYDRFVAALTPIRDAYERNDTASAIRQTMQLADEANKYIDETKPWIIAKQHHAD
AQLHAVCTQGLNLFRVLITALKPILPHTSIQAETFLAAPVTAWQDVNQPLTGGHTIQPYSPLFTRIDKKIIEVMINASKD
TLAPPPASAKQQNASMSNTAPPPTAEEPETTAPTIGIDDFAKLDLRIGKVLVCEYVEGSDKLLRFELDAGPLGKRQIFSG
IRASYSNPEALIGRNVVFIANLAPRKMRFGISQGMILSAGFDNGTLALLDADSSAQPGMPVR

Sequences:

>Translated_702_residues
MRYSHPLVPLSFMTTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLGGHNTWFVCADDTHGTPIMLAAEKAGMPPEA
FIATTQASHERDFAAFNVAFDHYDSTHSPVNRHLTEQSYLTLKQAGHITCRSVAQFYDPAKGMFLPDRYVKGTCPNCGAT
DQYGDNCEACGATYDPTELKNPYSVISGATPELRDSEHFFFEVAHFDTFLRHWLSGDVALPSVKNKLKEWLDAKGGLRPW
DISRDAPYFGFKIPDQPGKYFYVWLDAPIGYLCSFKTLCTRIGEDFDTHLRSGTTTELHHFIGKDIVNFHALFWPAVLHG
TGHRAPTRLHVNGYLTVDGAKMSKSRGTFIMARTYLDAGLEPDALRYYFAAKSSGDVDDLDLNLSDFVARVNADLVGKLV
NLASRCASFIGTRFNGQLADTLPDRIQYDRFVAALTPIRDAYERNDTASAIRQTMQLADEANKYIDETKPWIIAKQHHAD
AQLHAVCTQGLNLFRVLITALKPILPHTSIQAETFLAAPVTAWQDVNQPLTGGHTIQPYSPLFTRIDKKIIEVMINASKD
TLAPPPASAKQQNASMSNTAPPPTAEEPETTAPTIGIDDFAKLDLRIGKVLVCEYVEGSDKLLRFELDAGPLGKRQIFSG
IRASYSNPEALIGRNVVFIANLAPRKMRFGISQGMILSAGFDNGTLALLDADSSAQPGMPVR
>Mature_702_residues
MRYSHPLVPLSFMTTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLGGHNTWFVCADDTHGTPIMLAAEKAGMPPEA
FIATTQASHERDFAAFNVAFDHYDSTHSPVNRHLTEQSYLTLKQAGHITCRSVAQFYDPAKGMFLPDRYVKGTCPNCGAT
DQYGDNCEACGATYDPTELKNPYSVISGATPELRDSEHFFFEVAHFDTFLRHWLSGDVALPSVKNKLKEWLDAKGGLRPW
DISRDAPYFGFKIPDQPGKYFYVWLDAPIGYLCSFKTLCTRIGEDFDTHLRSGTTTELHHFIGKDIVNFHALFWPAVLHG
TGHRAPTRLHVNGYLTVDGAKMSKSRGTFIMARTYLDAGLEPDALRYYFAAKSSGDVDDLDLNLSDFVARVNADLVGKLV
NLASRCASFIGTRFNGQLADTLPDRIQYDRFVAALTPIRDAYERNDTASAIRQTMQLADEANKYIDETKPWIIAKQHHAD
AQLHAVCTQGLNLFRVLITALKPILPHTSIQAETFLAAPVTAWQDVNQPLTGGHTIQPYSPLFTRIDKKIIEVMINASKD
TLAPPPASAKQQNASMSNTAPPPTAEEPETTAPTIGIDDFAKLDLRIGKVLVCEYVEGSDKLLRFELDAGPLGKRQIFSG
IRASYSNPEALIGRNVVFIANLAPRKMRFGISQGMILSAGFDNGTLALLDADSSAQPGMPVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=611, Percent_Identity=28.3142389525368, Blast_Score=242, Evalue=7e-64,
Organism=Homo sapiens, GI24308436, Length=518, Percent_Identity=26.8339768339768, Blast_Score=143, Evalue=6e-34,
Organism=Escherichia coli, GI1788432, Length=688, Percent_Identity=53.7790697674419, Blast_Score=767, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=640, Percent_Identity=27.5, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=22.9126213592233, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71992781, Length=343, Percent_Identity=24.4897959183673, Blast_Score=89, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321704, Length=553, Percent_Identity=29.4755877034358, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321610, Length=541, Percent_Identity=24.3992606284658, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24655311, Length=580, Percent_Identity=28.2758620689655, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21358483, Length=538, Percent_Identity=25.6505576208178, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_XYLF2 (B2I7S0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830359.1
- ProteinModelPortal:   B2I7S0
- GeneID:   6202359
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1678
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77527; Mature: 77527

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSHPLVPLSFMTTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLGGHNTWFVCAD
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DTHGTPIMLAAEKAGMPPEAFIATTQASHERDFAAFNVAFDHYDSTHSPVNRHLTEQSYL
CCCCCEEEEEECCCCCCHHHEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHCHHHHH
TLKQAGHITCRSVAQFYDPAKGMFLPDRYVKGTCPNCGATDQYGDNCEACGATYDPTELK
HHHHCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCHHHCC
NPYSVISGATPELRDSEHFFFEVAHFDTFLRHWLSGDVALPSVKNKLKEWLDAKGGLRPW
CCHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
DISRDAPYFGFKIPDQPGKYFYVWLDAPIGYLCSFKTLCTRIGEDFDTHLRSGTTTELHH
CCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHH
FIGKDIVNFHALFWPAVLHGTGHRAPTRLHVNGYLTVDGAKMSKSRGTFIMARTYLDAGL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEHHHCCC
EPDALRYYFAAKSSGDVDDLDLNLSDFVARVNADLVGKLVNLASRCASFIGTRFNGQLAD
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TLPDRIQYDRFVAALTPIRDAYERNDTASAIRQTMQLADEANKYIDETKPWIIAKQHHAD
HCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
AQLHAVCTQGLNLFRVLITALKPILPHTSIQAETFLAAPVTAWQDVNQPLTGGHTIQPYS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCHHHHHHCCCCCCCCCCCCCCC
PLFTRIDKKIIEVMINASKDTLAPPPASAKQQNASMSNTAPPPTAEEPETTAPTIGIDDF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
AKLDLRIGKVLVCEYVEGSDKLLRFELDAGPLGKRQIFSGIRASYSNPEALIGRNVVFIA
HHHHHHHCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEE
NLAPRKMRFGISQGMILSAGFDNGTLALLDADSSAQPGMPVR
CCCCHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRYSHPLVPLSFMTTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLGGHNTWFVCAD
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DTHGTPIMLAAEKAGMPPEAFIATTQASHERDFAAFNVAFDHYDSTHSPVNRHLTEQSYL
CCCCCEEEEEECCCCCCHHHEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHCHHHHH
TLKQAGHITCRSVAQFYDPAKGMFLPDRYVKGTCPNCGATDQYGDNCEACGATYDPTELK
HHHHCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCHHHCC
NPYSVISGATPELRDSEHFFFEVAHFDTFLRHWLSGDVALPSVKNKLKEWLDAKGGLRPW
CCHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
DISRDAPYFGFKIPDQPGKYFYVWLDAPIGYLCSFKTLCTRIGEDFDTHLRSGTTTELHH
CCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHH
FIGKDIVNFHALFWPAVLHGTGHRAPTRLHVNGYLTVDGAKMSKSRGTFIMARTYLDAGL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEHHHCCC
EPDALRYYFAAKSSGDVDDLDLNLSDFVARVNADLVGKLVNLASRCASFIGTRFNGQLAD
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TLPDRIQYDRFVAALTPIRDAYERNDTASAIRQTMQLADEANKYIDETKPWIIAKQHHAD
HCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
AQLHAVCTQGLNLFRVLITALKPILPHTSIQAETFLAAPVTAWQDVNQPLTGGHTIQPYS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCHHHHHHCCCCCCCCCCCCCCC
PLFTRIDKKIIEVMINASKDTLAPPPASAKQQNASMSNTAPPPTAEEPETTAPTIGIDDF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
AKLDLRIGKVLVCEYVEGSDKLLRFELDAGPLGKRQIFSGIRASYSNPEALIGRNVVFIA
HHHHHHHCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEE
NLAPRKMRFGISQGMILSAGFDNGTLALLDADSSAQPGMPVR
CCCCHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA