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The map label for this gene is nadE [H]

Identifier: 182681436

GI number: 182681436

Start: 1048843

End: 1050480

Strand: Direct

Name: nadE [H]

Synonym: XfasM23_0887

Alternate gene names: 182681436

Gene position: 1048843-1050480 (Clockwise)

Preceding gene: 182681434

Following gene: 182681437

Centisome position: 41.36

GC content: 55.86

Gene sequence:

>1638_bases
ATGTCCGAATTTCTGCGTATTGCGATGGCGCAGTTTGATTTTCCTGTGGGTGCTGTGGCGCAAAATGCTGAGCGCATCAT
CGCGTTGATTGAGCAGGCGCGGGATGAGCACGGCGCTGATGTGGTGATGTTCCCAGAGTTGGCGCTCAGTGGTTATCCGC
CGGAAGATTTGCTGTTGCGTCCGGGTTTCCTAGCGCATTGTCAGGTGGCGATTGAACGCATCGCAGCGGCGACGCACGGT
ATTGTTGCAGTGGTTGGTTGGCCGCAAAGTGCGGGGAGTGTGGTGTACAACGTGGCCAGTGTGCTTTGTGACGGCCAGGT
TGAGCAGACTTACCGGAAGCGTGAGTTACCCAACTATGCGGTGTTCGACGAGCGCCGTTATTTTGAAGTTGATCCCAATG
GCAGTCGTTGTGTGTTCAAAGTAAAAGGGGTGCCGGTTGGGGTGTTGATCTGTGAGGATCTATGGTTCTCTGAGCCGCTG
GCCGATACGGTGTGTGGTGGTGCGGAGTTGGTGTTGGTGCCGAATGCTTCACCCTATGAGCGCGGGAAGCACGCGCAGCG
TGATGCGTTGTTAGCCGAGCGTGCGCGTGAGACGGGTGCAGCGATTGCATATCTCAATGTGGTGGGAGGTCAGGATGCGT
TGGTTTTTGATGGTGCTTCGGTAGTGGTTGATGGTCATGGCCGGGTGCATCCTGCGGCGGCAGCGTTCTCCGATCAGTGG
CTCGTGGTGGACTATATGCGTTCTGAGCGCCGTTTCGTCCCGTTGCAATGGGTAGTGGAGTCGGAGGTGAGCATCAATGC
GCTCGTGTGGCGTGCGGTGGTGCGTGGGGTGCAGGATTATTGCCGTAAGAATGGTTTTTCTAAGGTTTGGGTGGGGCTGT
CGGGGGGGATCGACTCGGCGTTGGTGCTTGCGATCGCTGTCGATGCGCTCGGTGCTGATCAGGTGACTGCGGTGCGGTTG
CCGTCGCGTTATACGGCTGAGTTGTCCAATGATTTGGCTGCGGAGCAGTGTCATAGTTTGGGGGTTAGGCTGGAAACGGT
GGCGATTGAGCCGGTATTTGAGGGGTTGTTAGCCGCATTGGGGCCGTTGTTTGCGGGAATGGCTCCAGATGCGACGGAGG
AGAATCTGCAATCGCGAAGCCGTGGGGTGATTTTGATGGCGTTGGCCAATAAGTTCGGTGGGCTGTTGTTGACGACGGGC
AATAAAAGTGAATATGCGGTTGGTTATGCGACGATCTACGGTGATATGTGTGGTGGGTATGCGCCGTTGAAAGATATTTA
TAAGAGCCAGGTGTTTGAGTTGGCTCAGTGGCGTAATAGGGTTAGCGATGTATTAGCGATCCCTCCTGGGGTGATTCATC
GGCCGCCTTCGGCGGAGTTGCGGGCGCAGCAGACTGATCAGGATTCGCTGCCACCCTATGAGGTGCTTGATGGCATTTTG
TCTCTCTATGTGGATCAAGAGCAGTCACGTGAGGACATCATCGCCGCTGGTTATGCGGCGGGTGTCGTTGACTATGTGCT
GAATTTGGTAAGGATCAATGAGTGGAAGCGTCATCAAGCAGCGCCGGGTCCGAAGGTGTCTCAGCGTGCGTTTGGACGAG
AGCGACGTTATCCGATTAGCAACGCTTATCGTGGCTAG

Upstream 100 bases:

>100_bases
GTGACTTTACTAAGTGGTGGGTGTGCAGGCTCTATTCATTCAGTGGGCGTTGACTGTTCTTGCCGGTGTGCAAGAGGGAT
ATGCATAAGGTTATTGAGAT

Downstream 100 bases:

>100_bases
GGGAGGGAGCGGGCGTGTTGAAGGGCGTGGTTGGTTTGCGATGCTGTGAGAGATGATGCGTTTTAGTAGGAATGCGAGTC
GTCCGTGAGATCAACAGGTG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSEFLRIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLRPGFLAHCQVAIERIAAATHG
IVAVVGWPQSAGSVVYNVASVLCDGQVEQTYRKRELPNYAVFDERRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPL
ADTVCGGAELVLVPNASPYERGKHAQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQW
LVVDYMRSERRFVPLQWVVESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLAIAVDALGADQVTAVRL
PSRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLFAGMAPDATEENLQSRSRGVILMALANKFGGLLLTTG
NKSEYAVGYATIYGDMCGGYAPLKDIYKSQVFELAQWRNRVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGIL
SLYVDQEQSREDIIAAGYAAGVVDYVLNLVRINEWKRHQAAPGPKVSQRAFGRERRYPISNAYRG

Sequences:

>Translated_545_residues
MSEFLRIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLRPGFLAHCQVAIERIAAATHG
IVAVVGWPQSAGSVVYNVASVLCDGQVEQTYRKRELPNYAVFDERRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPL
ADTVCGGAELVLVPNASPYERGKHAQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQW
LVVDYMRSERRFVPLQWVVESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLAIAVDALGADQVTAVRL
PSRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLFAGMAPDATEENLQSRSRGVILMALANKFGGLLLTTG
NKSEYAVGYATIYGDMCGGYAPLKDIYKSQVFELAQWRNRVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGIL
SLYVDQEQSREDIIAAGYAAGVVDYVLNLVRINEWKRHQAAPGPKVSQRAFGRERRYPISNAYRG
>Mature_544_residues
SEFLRIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLRPGFLAHCQVAIERIAAATHGI
VAVVGWPQSAGSVVYNVASVLCDGQVEQTYRKRELPNYAVFDERRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPLA
DTVCGGAELVLVPNASPYERGKHAQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQWL
VVDYMRSERRFVPLQWVVESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLAIAVDALGADQVTAVRLP
SRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLFAGMAPDATEENLQSRSRGVILMALANKFGGLLLTTGN
KSEYAVGYATIYGDMCGGYAPLKDIYKSQVFELAQWRNRVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGILS
LYVDQEQSREDIIAAGYAAGVVDYVLNLVRINEWKRHQAAPGPKVSQRAFGRERRYPISNAYRG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59373; Mature: 59242

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFLRIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCHHHEEC
PGFLAHCQVAIERIAAATHGIVAVVGWPQSAGSVVYNVASVLCDGQVEQTYRKRELPNYA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCE
VFDERRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPLADTVCGGAELVLVPNASPYE
EECCCEEEEECCCCCEEEEEECCCCEEEEEECHHHCCCCHHHHHCCCCEEEEECCCCCHH
RGKHAQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQW
CCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCCHHHHCCCCE
LVVDYMRSERRFVPLQWVVESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSA
EEEEEHHCCCCEECEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
LVLAIAVDALGADQVTAVRLPSRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAAL
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHH
GPLFAGMAPDATEENLQSRSRGVILMALANKFGGLLLTTGNKSEYAVGYATIYGDMCGGY
HHHHHCCCCCCCHHHHHHHCCCEEEEEEHHHHCCEEEEECCCCCEEEEEEEEEHHHCCCC
APLKDIYKSQVFELAQWRNRVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGIL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHH
SLYVDQEQSREDIIAAGYAAGVVDYVLNLVRINEWKRHQAAPGPKVSQRAFGRERRYPIS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCC
NAYRG
CCCCC
>Mature Secondary Structure 
SEFLRIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLR
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCHHHEEC
PGFLAHCQVAIERIAAATHGIVAVVGWPQSAGSVVYNVASVLCDGQVEQTYRKRELPNYA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCE
VFDERRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPLADTVCGGAELVLVPNASPYE
EECCCEEEEECCCCCEEEEEECCCCEEEEEECHHHCCCCHHHHHCCCCEEEEECCCCCHH
RGKHAQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQW
CCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCCHHHHCCCCE
LVVDYMRSERRFVPLQWVVESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSA
EEEEEHHCCCCEECEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
LVLAIAVDALGADQVTAVRLPSRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAAL
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHH
GPLFAGMAPDATEENLQSRSRGVILMALANKFGGLLLTTGNKSEYAVGYATIYGDMCGGY
HHHHHCCCCCCCHHHHHHHCCCEEEEEEHHHHCCEEEEECCCCCEEEEEEEEEHHHCCCC
APLKDIYKSQVFELAQWRNRVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGIL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHH
SLYVDQEQSREDIIAAGYAAGVVDYVLNLVRINEWKRHQAAPGPKVSQRAFGRERRYPIS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCC
NAYRG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]