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The map label for this gene is ahpF [H]

Identifier: 182681335

GI number: 182681335

Start: 929513

End: 931108

Strand: Direct

Name: ahpF [H]

Synonym: XfasM23_0782

Alternate gene names: 182681335

Gene position: 929513-931108 (Clockwise)

Preceding gene: 182681334

Following gene: 182681336

Centisome position: 36.66

GC content: 51.44

Gene sequence:

>1596_bases
ATGTTTGATGAAAGTTTGAAGGCTCAGCTGAAAGCCTATTTGGATCGGGTGATTCGCTCCATTCATATTCATGCTTCTGT
CGACGAGAGTCACAAGTCGCGTGAGATGCTCGATTTACTCGAAAATATTGTGCTGCTTTCGGGCAAGAAGGTCGTTCTGG
AGGTGCATCGTGACGATGATAAGCGAAAGCCATCCTTTGGGTTGAGTACTCCAGGAGAGAGTATTTCGATACGTTTTGCT
GGTTTGCCGATGGGGCACGAGTTTGCTTCATTGGTGCTTGGGTTGTTACAGGTTGGTGGGCATCCTCCTAAAGTGGCTGA
TGATCTTATTGAGCGGGTACGTAACCTGGATGGTCATTATCGTTTTGAGACCTATTTTTCAGTTTCTTGTCAGAGTTGCC
CGGATGTGGTGCAGGCACTGAATATGGCTGCGGTGTTGAATCCGCAGATTGAGCATGTGGCTATTGACGGTGCATTGTTC
CAGGATGAGGTTGAGGCGCGCCAGATTATGTCAGTACCAACGGTGTATTTGAATGGCGAGCTGTTTGGTCAGGGGCGTAT
GACGTTGGAACAGATTGTTGCCAAGCTTGATAGTGGTGCGGCCAAGCGCACAGCCGAGAGTATCCAGGCTAAGGCTGTTT
TTGACGTGTTGGTGGTTGGTGCCGGTCCGGCTGGTGTTGCAGCGGCGGTATATGCTGCGCGCAAGGGGGTCCGGACGGGT
TTGGTCGCTGAGCGCTTTGGTGGCCAAGTGTTGGATACCATGACGATCGAGAACTTCATATCTGTTCCGGAAACTGATGG
CCCGAGGATGGCTGCGGCGCTGGAGCAGCATGTGCGTCAGTATGAGGTCGATATCATGAATTTGCAGCGTGTCGAGACGT
TGGTACCGGCTGGAGAGAACGGCTTTATCGAGGTCAGATTGGCCAGTGGTGCGTCGCTGAGCAGCCGCACAGTGATTCTC
TCTACGGGTGCACGTTGGCGTCAGATGAATGTGCCTGGTGAGGATGAGTATCGAAACAAGGGGGTTGCTTATTGTCCGCA
TTGTGATGGCCCGTTGTTCAAGGGTAAGCGTGTCGCGGTGATTGGCGGTGGTAATTCGGGAGTCGAGGCAGCGATCGATT
TGGCTGGAATTGCTTCATATGTCACGTTGCTTGAGTTCGATACCAAACTGCGTGCCGACGAAGTATTGCAACGCAAGCTA
CGCAGTATGTCGAATGTGGAAGTCATCATGGGTGCCCAGACCACCGAGGTACGTGGTGATGGAAAGAAGGTGACTGGTTT
GGTCTATGTTGATCGCAGTACTGGTGGAAGCTTTATATTGGCGTTGGAAGGAGTGTTTGTGCAGATTGGTCTGTTACCCA
ATACCGAGTGGTTGAAAGGTAGGGTGGAATTGTCTCCCCGTGGAGAAATTGTCGTTGATGAACGTGGTCATACCAGCTTA
CCAGGTGTGTTCGCTGCTGGTGATGCGACCACGGTGCCGTATAAGCAGATTGTTATTGCGATGGGGGAGGGGTCTAAAGC
CGCGTTGAGTGCTTTCGATTATCTGATTCGTAATGTGGTTGAAATTACTGGAATTGCTCAGGCACAGGCAGCGTGA

Upstream 100 bases:

>100_bases
TGGGGATATATTTGCTGTTGATTCCCTTTATATATCCTCGTCGGCGATTTGCCGTGCAGCGACGCCGTGTGCTTTTAATC
CTCCTCTCCAGGAGATAGTT

Downstream 100 bases:

>100_bases
GCCAATGCAGTACAGCGGTTTAACTTTATGTTGCTTAGTCTTGATGTTGTTGCAATACCTAAGTGTGGAATTACATTCCC
TCCTTTGCCGCGAGGTTTGG

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MFDESLKAQLKAYLDRVIRSIHIHASVDESHKSREMLDLLENIVLLSGKKVVLEVHRDDDKRKPSFGLSTPGESISIRFA
GLPMGHEFASLVLGLLQVGGHPPKVADDLIERVRNLDGHYRFETYFSVSCQSCPDVVQALNMAAVLNPQIEHVAIDGALF
QDEVEARQIMSVPTVYLNGELFGQGRMTLEQIVAKLDSGAAKRTAESIQAKAVFDVLVVGAGPAGVAAAVYAARKGVRTG
LVAERFGGQVLDTMTIENFISVPETDGPRMAAALEQHVRQYEVDIMNLQRVETLVPAGENGFIEVRLASGASLSSRTVIL
STGARWRQMNVPGEDEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIASYVTLLEFDTKLRADEVLQRKL
RSMSNVEVIMGAQTTEVRGDGKKVTGLVYVDRSTGGSFILALEGVFVQIGLLPNTEWLKGRVELSPRGEIVVDERGHTSL
PGVFAAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRNVVEITGIAQAQAA

Sequences:

>Translated_531_residues
MFDESLKAQLKAYLDRVIRSIHIHASVDESHKSREMLDLLENIVLLSGKKVVLEVHRDDDKRKPSFGLSTPGESISIRFA
GLPMGHEFASLVLGLLQVGGHPPKVADDLIERVRNLDGHYRFETYFSVSCQSCPDVVQALNMAAVLNPQIEHVAIDGALF
QDEVEARQIMSVPTVYLNGELFGQGRMTLEQIVAKLDSGAAKRTAESIQAKAVFDVLVVGAGPAGVAAAVYAARKGVRTG
LVAERFGGQVLDTMTIENFISVPETDGPRMAAALEQHVRQYEVDIMNLQRVETLVPAGENGFIEVRLASGASLSSRTVIL
STGARWRQMNVPGEDEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIASYVTLLEFDTKLRADEVLQRKL
RSMSNVEVIMGAQTTEVRGDGKKVTGLVYVDRSTGGSFILALEGVFVQIGLLPNTEWLKGRVELSPRGEIVVDERGHTSL
PGVFAAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRNVVEITGIAQAQAA
>Mature_531_residues
MFDESLKAQLKAYLDRVIRSIHIHASVDESHKSREMLDLLENIVLLSGKKVVLEVHRDDDKRKPSFGLSTPGESISIRFA
GLPMGHEFASLVLGLLQVGGHPPKVADDLIERVRNLDGHYRFETYFSVSCQSCPDVVQALNMAAVLNPQIEHVAIDGALF
QDEVEARQIMSVPTVYLNGELFGQGRMTLEQIVAKLDSGAAKRTAESIQAKAVFDVLVVGAGPAGVAAAVYAARKGVRTG
LVAERFGGQVLDTMTIENFISVPETDGPRMAAALEQHVRQYEVDIMNLQRVETLVPAGENGFIEVRLASGASLSSRTVIL
STGARWRQMNVPGEDEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIASYVTLLEFDTKLRADEVLQRKL
RSMSNVEVIMGAQTTEVRGDGKKVTGLVYVDRSTGGSFILALEGVFVQIGLLPNTEWLKGRVELSPRGEIVVDERGHTSL
PGVFAAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRNVVEITGIAQAQAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=59.6525096525097, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=317, Percent_Identity=35.0157728706625, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321898, Length=311, Percent_Identity=33.1189710610932, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=31.8327974276527, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 57297; Mature: 57297

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDESLKAQLKAYLDRVIRSIHIHASVDESHKSREMLDLLENIVLLSGKKVVLEVHRDDD
CCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
KRKPSFGLSTPGESISIRFAGLPMGHEFASLVLGLLQVGGHPPKVADDLIERVRNLDGHY
CCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCE
RFETYFSVSCQSCPDVVQALNMAAVLNPQIEHVAIDGALFQDEVEARQIMSVPTVYLNGE
EEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEECCE
LFGQGRMTLEQIVAKLDSGAAKRTAESIQAKAVFDVLVVGAGPAGVAAAVYAARKGVRTG
EECCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHCCHHH
LVAERFGGQVLDTMTIENFISVPETDGPRMAAALEQHVRQYEVDIMNLQRVETLVPAGEN
HHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GFIEVRLASGASLSSRTVILSTGARWRQMNVPGEDEYRNKGVAYCPHCDGPLFKGKRVAV
CEEEEEEECCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIASYVTLLEFDTKLRADEVLQRKLRSMSNVEVIMGAQTTEVRGD
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCC
GKKVTGLVYVDRSTGGSFILALEGVFVQIGLLPNTEWLKGRVELSPRGEIVVDERGHTSL
CCEEEEEEEEECCCCCCEEEEEEEEEEEEEECCCCHHHCEEEEECCCCCEEEECCCCCCC
PGVFAAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRNVVEITGIAQAQAA
CCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MFDESLKAQLKAYLDRVIRSIHIHASVDESHKSREMLDLLENIVLLSGKKVVLEVHRDDD
CCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
KRKPSFGLSTPGESISIRFAGLPMGHEFASLVLGLLQVGGHPPKVADDLIERVRNLDGHY
CCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCE
RFETYFSVSCQSCPDVVQALNMAAVLNPQIEHVAIDGALFQDEVEARQIMSVPTVYLNGE
EEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEECCE
LFGQGRMTLEQIVAKLDSGAAKRTAESIQAKAVFDVLVVGAGPAGVAAAVYAARKGVRTG
EECCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHCCHHH
LVAERFGGQVLDTMTIENFISVPETDGPRMAAALEQHVRQYEVDIMNLQRVETLVPAGEN
HHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GFIEVRLASGASLSSRTVILSTGARWRQMNVPGEDEYRNKGVAYCPHCDGPLFKGKRVAV
CEEEEEEECCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIASYVTLLEFDTKLRADEVLQRKLRSMSNVEVIMGAQTTEVRGD
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCC
GKKVTGLVYVDRSTGGSFILALEGVFVQIGLLPNTEWLKGRVELSPRGEIVVDERGHTSL
CCEEEEEEEEECCCCCCEEEEEEEEEEEEEECCCCHHHCEEEEECCCCCEEEECCCCCCC
PGVFAAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRNVVEITGIAQAQAA
CCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9190810 [H]