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The map label for this gene is truB [H]

Identifier: 197294801

GI number: 197294801

Start: 804983

End: 805837

Strand: Reverse

Name: truB [H]

Synonym: PAa_0783

Alternate gene names: 197294801

Gene position: 805837-804983 (Counterclockwise)

Preceding gene: 197294802

Following gene: 197294800

Centisome position: 91.58

GC content: 28.89

Gene sequence:

>855_bases
ATGAATGGTTTCTTTTTAGTTCACAAACCTTCCCAACTCACTTCTCATGATGTAGTTTATCAAATTAAAAAAAAATTTCA
CCTTGATAAGGTGGGTCACACAGGAACACTAGATCCTTTAGCGTCAGGACTTTTAATTGTTTGTGTTGGTAAGGCCACAA
AGTTATCTTTTTTGTTTGATGGCCTTGACAAAACTTATCAAGGAACTTTTATTTTTAATCGCAAATATGATACTTTAGAT
GTGACAGGTAAGTTATTGGATACTAAAGTAAATATTTTAAACCAACAATTAATCCAAAAAACTTTTGCCTCTTTTCATCA
AAAAAGTTATTTCCAAACACCTCCTATGTTTTCAGCGATCAAAGTCAATGGCCAAAAAATGTATCATTTAGCTCGCAAAA
ATAAAATCATTGACCTTCCACCTAAAAAAGTTATGATTAATCATTTAGAAACCCAATCTTTATTAAAAAATGATCAAATT
GATTTTTTAACAACAGTTTCTAAAGGAACTTATATTCGTAGTTTGGCGCAAGATATAGCTTCAAAAATGAATACTTATGG
TGCACTACTTTCTTTGAAACGTGTGGCTATTGGACTTTATTTATTGCAAAATGCCAAAACAGTGGATGATTTAACCAGGG
ATGATTTTATTTTGGATTCTTCTTTATTTGAATCTTGCGATAAAATTATTTTAAATGACTATTTAATTAAATTAGTTCAA
AATGGTGTTTATTTAGATCAAAGGCAAACAACAACTGAAAAACCTTTTATTGTGCAAGATAGTAACCATAATTTGATTGC
TTATTATGATGTTTTGGAAAAAAATAAATATTATCCGCGGTATTTTTTTAAATAA

Upstream 100 bases:

>100_bases
AGAAAGAAGTAGCTAGAAATAACGGTTTCTTAGATAAAGAAGAATTATTATCTTTTCTCCAAAAACATAAATAATGTTAT
CATGTTCCAATAAAAATAAA

Downstream 100 bases:

>100_bases
TGAATATTTTTAGTTTTAAAATGACTAAGATGACTAATGAAAGTTTGAAATATTGTTTATGGAAAGATTACAAAAATTTA
TAGCATCAGCTAATTTAGTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNGFFLVHKPSQLTSHDVVYQIKKKFHLDKVGHTGTLDPLASGLLIVCVGKATKLSFLFDGLDKTYQGTFIFNRKYDTLD
VTGKLLDTKVNILNQQLIQKTFASFHQKSYFQTPPMFSAIKVNGQKMYHLARKNKIIDLPPKKVMINHLETQSLLKNDQI
DFLTTVSKGTYIRSLAQDIASKMNTYGALLSLKRVAIGLYLLQNAKTVDDLTRDDFILDSSLFESCDKIILNDYLIKLVQ
NGVYLDQRQTTTEKPFIVQDSNHNLIAYYDVLEKNKYYPRYFFK

Sequences:

>Translated_284_residues
MNGFFLVHKPSQLTSHDVVYQIKKKFHLDKVGHTGTLDPLASGLLIVCVGKATKLSFLFDGLDKTYQGTFIFNRKYDTLD
VTGKLLDTKVNILNQQLIQKTFASFHQKSYFQTPPMFSAIKVNGQKMYHLARKNKIIDLPPKKVMINHLETQSLLKNDQI
DFLTTVSKGTYIRSLAQDIASKMNTYGALLSLKRVAIGLYLLQNAKTVDDLTRDDFILDSSLFESCDKIILNDYLIKLVQ
NGVYLDQRQTTTEKPFIVQDSNHNLIAYYDVLEKNKYYPRYFFK
>Mature_284_residues
MNGFFLVHKPSQLTSHDVVYQIKKKFHLDKVGHTGTLDPLASGLLIVCVGKATKLSFLFDGLDKTYQGTFIFNRKYDTLD
VTGKLLDTKVNILNQQLIQKTFASFHQKSYFQTPPMFSAIKVNGQKMYHLARKNKIIDLPPKKVMINHLETQSLLKNDQI
DFLTTVSKGTYIRSLAQDIASKMNTYGALLSLKRVAIGLYLLQNAKTVDDLTRDDFILDSSLFESCDKIILNDYLIKLVQ
NGVYLDQRQTTTEKPFIVQDSNHNLIAYYDVLEKNKYYPRYFFK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=260, Percent_Identity=28.4615384615385, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI4503337, Length=268, Percent_Identity=26.1194029850746, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI215599015, Length=268, Percent_Identity=26.1194029850746, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI2367200, Length=213, Percent_Identity=30.5164319248826, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17553978, Length=268, Percent_Identity=28.7313432835821, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=264, Percent_Identity=27.6515151515151, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=190, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32643; Mature: 32643

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFFLVHKPSQLTSHDVVYQIKKKFHLDKVGHTGTLDPLASGLLIVCVGKATKLSFLFD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCHHHHHHHH
GLDKTYQGTFIFNRKYDTLDVTGKLLDTKVNILNQQLIQKTFASFHQKSYFQTPPMFSAI
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
KVNGQKMYHLARKNKIIDLPPKKVMINHLETQSLLKNDQIDFLTTVSKGTYIRSLAQDIA
EECCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHH
SKMNTYGALLSLKRVAIGLYLLQNAKTVDDLTRDDFILDSSLFESCDKIILNDYLIKLVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHH
NGVYLDQRQTTTEKPFIVQDSNHNLIAYYDVLEKNKYYPRYFFK
CCCEECCCCCCCCCCEEEECCCCCEEEEEEEHHCCCCCCCCCCC
>Mature Secondary Structure
MNGFFLVHKPSQLTSHDVVYQIKKKFHLDKVGHTGTLDPLASGLLIVCVGKATKLSFLFD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCHHHHHHHH
GLDKTYQGTFIFNRKYDTLDVTGKLLDTKVNILNQQLIQKTFASFHQKSYFQTPPMFSAI
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
KVNGQKMYHLARKNKIIDLPPKKVMINHLETQSLLKNDQIDFLTTVSKGTYIRSLAQDIA
EECCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHH
SKMNTYGALLSLKRVAIGLYLLQNAKTVDDLTRDDFILDSSLFESCDKIILNDYLIKLVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHH
NGVYLDQRQTTTEKPFIVQDSNHNLIAYYDVLEKNKYYPRYFFK
CCCEECCCCCCCCCCEEEECCCCCEEEEEEEHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA