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The map label for this gene is rsuA [H]

Identifier: 197294800

GI number: 197294800

Start: 804193

End: 804924

Strand: Reverse

Name: rsuA [H]

Synonym: PAa_0782

Alternate gene names: 197294800

Gene position: 804924-804193 (Counterclockwise)

Preceding gene: 197294801

Following gene: 197294799

Centisome position: 91.47

GC content: 25.41

Gene sequence:

>732_bases
ATGGAAAGATTACAAAAATTTATAGCATCAGCTAATTTAGTTTCTAGAAGGAAAGTAGAAATTTTAATTTTACAAAAAAG
AGTAAAAGTAAATAATCAAGTTGTTTCTCAATTAGGCACTAAGGTTAAAGCTTCTGATGTTGTTACTTTAGATGATGTTT
TAATTAAAAAAACTACTTTATTTTATTATTTACTTAACAAACCTCAAGGATACCTTTCAACTACTAAAGATGATAAAAAA
AGGCCCACCGTATTAGATTTAATCAAAGAGTCTCAAAACCAAAGATTATATTCTGTTGGAAGATTAGATTTTAATACCAC
AGGTTTAATTTTAATTACTAACGATGGCGAGATGACTCAAAAATTAACTCATCCTTGTTTTGGTATTCCTAAAGAATATC
ATGTTAAAATAAATGTTTTTTTAACTAAAAAAGACCTTTCGTTGTTGCTTAAAGGTGTTATTATTGATCACAATTATTTA
TCAATTCCCAAAGAAATTAATATTTTAAAACAACGTTATTTACCCAAACCATCTACTTGGCTTAAAATAGTAATCACTGA
AGGAAAAAACCGTCAAATAAGAAAAATGATGGAAGCTTTAGGATATCAAGTTTTAAAATTAAGACGTTATAGTTATGCTT
TTTTAACTATTGAAGGATTAAAAACAGGTGCTTATCGTCTTTTAAAAATTCACGAAATCAAAAAATTAAAAATTTTAACT
AATAAAAACTAA

Upstream 100 bases:

>100_bases
TTGGAAAAAAATAAATATTATCCGCGGTATTTTTTTAAATAATGAATATTTTTAGTTTTAAAATGACTAAGATGACTAAT
GAAAGTTTGAAATATTGTTT

Downstream 100 bases:

>100_bases
AATGATTTTTTTAAGGAGTCAATATTTTGTATGCAAGGTTTTAAATTAGCAATTGATGGGCCTGCTGGAGCGGGTAAAAG
TACCGTTTGTCAAAAACTGT

Product: 16S rRNA pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MERLQKFIASANLVSRRKVEILILQKRVKVNNQVVSQLGTKVKASDVVTLDDVLIKKTTLFYYLLNKPQGYLSTTKDDKK
RPTVLDLIKESQNQRLYSVGRLDFNTTGLILITNDGEMTQKLTHPCFGIPKEYHVKINVFLTKKDLSLLLKGVIIDHNYL
SIPKEINILKQRYLPKPSTWLKIVITEGKNRQIRKMMEALGYQVLKLRRYSYAFLTIEGLKTGAYRLLKIHEIKKLKILT
NKN

Sequences:

>Translated_243_residues
MERLQKFIASANLVSRRKVEILILQKRVKVNNQVVSQLGTKVKASDVVTLDDVLIKKTTLFYYLLNKPQGYLSTTKDDKK
RPTVLDLIKESQNQRLYSVGRLDFNTTGLILITNDGEMTQKLTHPCFGIPKEYHVKINVFLTKKDLSLLLKGVIIDHNYL
SIPKEINILKQRYLPKPSTWLKIVITEGKNRQIRKMMEALGYQVLKLRRYSYAFLTIEGLKTGAYRLLKIHEIKKLKILT
NKN
>Mature_243_residues
MERLQKFIASANLVSRRKVEILILQKRVKVNNQVVSQLGTKVKASDVVTLDDVLIKKTTLFYYLLNKPQGYLSTTKDDKK
RPTVLDLIKESQNQRLYSVGRLDFNTTGLILITNDGEMTQKLTHPCFGIPKEYHVKINVFLTKKDLSLLLKGVIIDHNYL
SIPKEINILKQRYLPKPSTWLKIVITEGKNRQIRKMMEALGYQVLKLRRYSYAFLTIEGLKTGAYRLLKIHEIKKLKILT
NKN

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=32.9317269076305, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=32.7731092436975, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790453, Length=245, Percent_Identity=31.0204081632653, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=30.6818181818182, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 28173; Mature: 28173

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKFIASANLVSRRKVEILILQKRVKVNNQVVSQLGTKVKASDVVTLDDVLIKKTTL
CHHHHHHHHHCCHHHCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHEE
FYYLLNKPQGYLSTTKDDKKRPTVLDLIKESQNQRLYSVGRLDFNTTGLILITNDGEMTQ
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECEEECCCEEEEEECCCHHHH
KLTHPCFGIPKEYHVKINVFLTKKDLSLLLKGVIIDHNYLSIPKEINILKQRYLPKPSTW
HHCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCE
LKIVITEGKNRQIRKMMEALGYQVLKLRRYSYAFLTIEGLKTGAYRLLKIHEIKKLKILT
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEEECCEEEEEEE
NKN
CCC
>Mature Secondary Structure
MERLQKFIASANLVSRRKVEILILQKRVKVNNQVVSQLGTKVKASDVVTLDDVLIKKTTL
CHHHHHHHHHCCHHHCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHEE
FYYLLNKPQGYLSTTKDDKKRPTVLDLIKESQNQRLYSVGRLDFNTTGLILITNDGEMTQ
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECEEECCCEEEEEECCCHHHH
KLTHPCFGIPKEYHVKINVFLTKKDLSLLLKGVIIDHNYLSIPKEINILKQRYLPKPSTW
HHCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCE
LKIVITEGKNRQIRKMMEALGYQVLKLRRYSYAFLTIEGLKTGAYRLLKIHEIKKLKILT
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEEECCEEEEEEE
NKN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]