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The map label for this gene is ligA

Identifier: 197294788

GI number: 197294788

Start: 789253

End: 791265

Strand: Direct

Name: ligA

Synonym: PAa_0770

Alternate gene names: 197294788

Gene position: 789253-791265 (Clockwise)

Preceding gene: 197294785

Following gene: 197294789

Centisome position: 89.69

GC content: 28.91

Gene sequence:

>2013_bases
ATGATAAATTTAGAATTAATAGAAAAAAAAATAAAAGATTTAACAAACCAACTTAATGAAGCTAATTATGAATATTATGT
TAAAAGCAATCCAAAACTTACAGACCAACAATACGATGCTTTATTAAAAGAATTGCTTGTTTTAGAAAAAAAATTCCCTC
AATTCACCCTTCCTTATAGTCCTACTTTAAAAATAGGTGGTTTTGTTGAAAAAAAATTTTCTACTGTTAAACACACGAGT
GCCATGATGTCTTTAGCAAATGCCTTTAACTTAAAAGAATTAAAAGCTTTTTATGACCGTATTGCTAAAAAATTTTCGTC
TTTTAGTATGGTATCTGAATTAAAAATTGATGGTGTGGCTGTCAGTTTAAAATATAAAAAAGGTATTTTATTCCAAGCAT
TAACTAGAGGAAACGGTGTTTGGGGAGAAGAAATTACGAAAAACGTACAAACAATCAAGCAAGTCCCTTTAAAATTAACT
CAACCGTTAGATTTAGAAGTAAGAGGAGAAATTTATTTATCCAACTCCTCTTTTCAAAAACTAAACCAACAAAGAAAAAT
AGCGCAACAACCTCTTTTTTCCAATCCTAGGAATGCCGCTTCAGGAACTATTAGGCAACTAAATTCTTCGATAGTGGCCC
AAAGAAACTTATCTATTTTTATTTATTCTATCCAAAATCCTTATCTTCTCAAGTCCACCCAAGAAGAAACACTTACTTTT
TTAGCTTCCTTGGGTTTTAGCGTCAATCCTTATTATAAGTGTAGTTCTAGTTTTGAAGAATTGGAAATTAAAATCCAAGA
ATTAGAAAAATTACAGCAAAATCTTGATTATAATACTGACGGGGTAGTTGTCAAAATTAACGAATTAAACTTACATGATT
CAATAGGGAAAACAACTAAATCCCCTAAATGGGCTATTGCTTACAAATTTGCGACCAAAAATAGCGAAAGTGTTGTCCAA
GAAATTATTTTTCAAGTAGGTCGTTCAGGAATGTTGACTCCTGTTTGTAAAATTATCCCTGTAATGGTTGATGGTAGTTT
AGTTTCTAAAGTGGTTCTACATAATTATGACTATATTTGTAAAAAAGATATCCGTTTAAAAGATTATGTTCAAGTTCACA
AAGCAGGATCTGTTATTCCTGAAATAAAAGAAGTGATTAAAAGCAAAAGGCCTTCTGATTCCAAACCCTTTGCAATGATT
ACTCAATGCCCTTCTTGTCATTCATTATTAAATAAAAACAAAGGAGAAGTTGATTATTTCTGTATTAACAAAAACTGCTT
GGAGCAAAAAATTCAAAAATTAATTCATTTTGTTTCCAAAAATGCTATGGATATCGATACTTTAGGAAATCAAACTTTAA
TTACTTTTTTTAATCAAAAACTAATTGAAAATCCCTCTGATATATACTTATTAAAAAATAATTTAGATGTTTTGCAAAAA
ATAAATGGTTTTGGGGCAAAAAAAGTGCAAAACATTTTAACATCCGTTGAAAAAAGTAAAAATAAAAGCTTGGAAAACGT
TTTATTTGGTTTAGGAATTAAACATGTAGGCCTCAAAGTATCTCAAGTATTAACTAAACATTTTGATTCCATAGAAATAC
TTCAAAAAACTACTTTAGAAAATTTAGAATCCATTAAATCGATTCCTGAAATAGGAGAAAAAATTGCTTTAAGTCTTCAA
AAATATTTTCAAAATCCTTTGCATCTAGAAGAAATAACAAAATTAAAACAGTTGGGAGTTTCTTTTAAAAGCACAACTAC
ATCACAAATAAAGGCTCCTAATTTTTTCACCAATAAAAAAGTTGTTTTAACAGGGTCTTTACAAAATTACACCCGCAGCC
AAATAAAAAAACTTTTGATTCAAAAAGGAGCCATTATTAATGACGCCCTTTCTTCACAAACACATTTATTAATAACAGGG
GCAAATCCCGGATCTAAATTACAAAAGGCAAAAACACTAAACATCCAAATTATAGAAGAAAAAGAATTAGAAGAATTAAT
CCAAGAAAAATAA

Upstream 100 bases:

>100_bases
AAAACAACTGCAAATGAAAAATTAAATTAATCAGTTTTTATCGCCCAAAATTTTATCACCCCAAAAACTTTCTAAAAAAT
TAGTAAGGTCCTTGATTTCC

Downstream 100 bases:

>100_bases
AAAGATAAAAAATTAAAGAGGGACTAATGTCTTTTACTATCCAAATAAAAGAAGAAATTGCTTTACAAAAGTTATCACCT
TTAGAAAATTTTTTTGAACT

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MINLELIEKKIKDLTNQLNEANYEYYVKSNPKLTDQQYDALLKELLVLEKKFPQFTLPYSPTLKIGGFVEKKFSTVKHTS
AMMSLANAFNLKELKAFYDRIAKKFSSFSMVSELKIDGVAVSLKYKKGILFQALTRGNGVWGEEITKNVQTIKQVPLKLT
QPLDLEVRGEIYLSNSSFQKLNQQRKIAQQPLFSNPRNAASGTIRQLNSSIVAQRNLSIFIYSIQNPYLLKSTQEETLTF
LASLGFSVNPYYKCSSSFEELEIKIQELEKLQQNLDYNTDGVVVKINELNLHDSIGKTTKSPKWAIAYKFATKNSESVVQ
EIIFQVGRSGMLTPVCKIIPVMVDGSLVSKVVLHNYDYICKKDIRLKDYVQVHKAGSVIPEIKEVIKSKRPSDSKPFAMI
TQCPSCHSLLNKNKGEVDYFCINKNCLEQKIQKLIHFVSKNAMDIDTLGNQTLITFFNQKLIENPSDIYLLKNNLDVLQK
INGFGAKKVQNILTSVEKSKNKSLENVLFGLGIKHVGLKVSQVLTKHFDSIEILQKTTLENLESIKSIPEIGEKIALSLQ
KYFQNPLHLEEITKLKQLGVSFKSTTTSQIKAPNFFTNKKVVLTGSLQNYTRSQIKKLLIQKGAIINDALSSQTHLLITG
ANPGSKLQKAKTLNIQIIEEKELEELIQEK

Sequences:

>Translated_670_residues
MINLELIEKKIKDLTNQLNEANYEYYVKSNPKLTDQQYDALLKELLVLEKKFPQFTLPYSPTLKIGGFVEKKFSTVKHTS
AMMSLANAFNLKELKAFYDRIAKKFSSFSMVSELKIDGVAVSLKYKKGILFQALTRGNGVWGEEITKNVQTIKQVPLKLT
QPLDLEVRGEIYLSNSSFQKLNQQRKIAQQPLFSNPRNAASGTIRQLNSSIVAQRNLSIFIYSIQNPYLLKSTQEETLTF
LASLGFSVNPYYKCSSSFEELEIKIQELEKLQQNLDYNTDGVVVKINELNLHDSIGKTTKSPKWAIAYKFATKNSESVVQ
EIIFQVGRSGMLTPVCKIIPVMVDGSLVSKVVLHNYDYICKKDIRLKDYVQVHKAGSVIPEIKEVIKSKRPSDSKPFAMI
TQCPSCHSLLNKNKGEVDYFCINKNCLEQKIQKLIHFVSKNAMDIDTLGNQTLITFFNQKLIENPSDIYLLKNNLDVLQK
INGFGAKKVQNILTSVEKSKNKSLENVLFGLGIKHVGLKVSQVLTKHFDSIEILQKTTLENLESIKSIPEIGEKIALSLQ
KYFQNPLHLEEITKLKQLGVSFKSTTTSQIKAPNFFTNKKVVLTGSLQNYTRSQIKKLLIQKGAIINDALSSQTHLLITG
ANPGSKLQKAKTLNIQIIEEKELEELIQEK
>Mature_670_residues
MINLELIEKKIKDLTNQLNEANYEYYVKSNPKLTDQQYDALLKELLVLEKKFPQFTLPYSPTLKIGGFVEKKFSTVKHTS
AMMSLANAFNLKELKAFYDRIAKKFSSFSMVSELKIDGVAVSLKYKKGILFQALTRGNGVWGEEITKNVQTIKQVPLKLT
QPLDLEVRGEIYLSNSSFQKLNQQRKIAQQPLFSNPRNAASGTIRQLNSSIVAQRNLSIFIYSIQNPYLLKSTQEETLTF
LASLGFSVNPYYKCSSSFEELEIKIQELEKLQQNLDYNTDGVVVKINELNLHDSIGKTTKSPKWAIAYKFATKNSESVVQ
EIIFQVGRSGMLTPVCKIIPVMVDGSLVSKVVLHNYDYICKKDIRLKDYVQVHKAGSVIPEIKEVIKSKRPSDSKPFAMI
TQCPSCHSLLNKNKGEVDYFCINKNCLEQKIQKLIHFVSKNAMDIDTLGNQTLITFFNQKLIENPSDIYLLKNNLDVLQK
INGFGAKKVQNILTSVEKSKNKSLENVLFGLGIKHVGLKVSQVLTKHFDSIEILQKTTLENLESIKSIPEIGEKIALSLQ
KYFQNPLHLEEITKLKQLGVSFKSTTTSQIKAPNFFTNKKVVLTGSLQNYTRSQIKKLLIQKGAIINDALSSQTHLLITG
ANPGSKLQKAKTLNIQIIEEKELEELIQEK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=677, Percent_Identity=34.711964549483, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI87082305, Length=517, Percent_Identity=24.9516441005803, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PHYAS (B1VAY1)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799329.1
- ProteinModelPortal:   B1VAY1
- GeneID:   6799326
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK343750
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75822; Mature: 75822

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 170-170 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLELIEKKIKDLTNQLNEANYEYYVKSNPKLTDQQYDALLKELLVLEKKFPQFTLPYS
CCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
PTLKIGGFVEKKFSTVKHTSAMMSLANAFNLKELKAFYDRIAKKFSSFSMVSELKIDGVA
CCEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEE
VSLKYKKGILFQALTRGNGVWGEEITKNVQTIKQVPLKLTQPLDLEVRGEIYLSNSSFQK
EEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCCCCCEEEECEEEECCCHHHH
LNQQRKIAQQPLFSNPRNAASGTIRQLNSSIVAQRNLSIFIYSIQNPYLLKSTQEETLTF
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEEECCCEEECCCHHHHHHH
LASLGFSVNPYYKCSSSFEELEIKIQELEKLQQNLDYNTDGVVVKINELNLHDSIGKTTK
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHCCCCC
SPKWAIAYKFATKNSESVVQEIIFQVGRSGMLTPVCKIIPVMVDGSLVSKVVLHNYDYIC
CCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHH
KKDIRLKDYVQVHKAGSVIPEIKEVIKSKRPSDSKPFAMITQCPSCHSLLNKNKGEVDYF
CCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHCCCCCCEEEE
CINKNCLEQKIQKLIHFVSKNAMDIDTLGNQTLITFFNQKLIENPSDIYLLKNNLDVLQK
EECHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHH
INGFGAKKVQNILTSVEKSKNKSLENVLFGLGIKHVGLKVSQVLTKHFDSIEILQKTTLE
HCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NLESIKSIPEIGEKIALSLQKYFQNPLHLEEITKLKQLGVSFKSTTTSQIKAPNFFTNKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
VVLTGSLQNYTRSQIKKLLIQKGAIINDALSSQTHLLITGANPGSKLQKAKTLNIQIIEE
EEEECCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEECH
KELEELIQEK
HHHHHHHHCC
>Mature Secondary Structure
MINLELIEKKIKDLTNQLNEANYEYYVKSNPKLTDQQYDALLKELLVLEKKFPQFTLPYS
CCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
PTLKIGGFVEKKFSTVKHTSAMMSLANAFNLKELKAFYDRIAKKFSSFSMVSELKIDGVA
CCEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEE
VSLKYKKGILFQALTRGNGVWGEEITKNVQTIKQVPLKLTQPLDLEVRGEIYLSNSSFQK
EEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCCCCCEEEECEEEECCCHHHH
LNQQRKIAQQPLFSNPRNAASGTIRQLNSSIVAQRNLSIFIYSIQNPYLLKSTQEETLTF
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEEECCCEEECCCHHHHHHH
LASLGFSVNPYYKCSSSFEELEIKIQELEKLQQNLDYNTDGVVVKINELNLHDSIGKTTK
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHCCCCC
SPKWAIAYKFATKNSESVVQEIIFQVGRSGMLTPVCKIIPVMVDGSLVSKVVLHNYDYIC
CCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHH
KKDIRLKDYVQVHKAGSVIPEIKEVIKSKRPSDSKPFAMITQCPSCHSLLNKNKGEVDYF
CCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHCCCCCCEEEE
CINKNCLEQKIQKLIHFVSKNAMDIDTLGNQTLITFFNQKLIENPSDIYLLKNNLDVLQK
EECHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHH
INGFGAKKVQNILTSVEKSKNKSLENVLFGLGIKHVGLKVSQVLTKHFDSIEILQKTTLE
HCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NLESIKSIPEIGEKIALSLQKYFQNPLHLEEITKLKQLGVSFKSTTTSQIKAPNFFTNKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
VVLTGSLQNYTRSQIKKLLIQKGAIINDALSSQTHLLITGANPGSKLQKAKTLNIQIIEE
EEEECCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEECH
KELEELIQEK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA