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The map label for this gene is sfcA [C]

Identifier: 197294787

GI number: 197294787

Start: 786806

End: 787978

Strand: Reverse

Name: sfcA [C]

Synonym: PAa_0769

Alternate gene names: 197294787

Gene position: 787978-786806 (Counterclockwise)

Preceding gene: 197294792

Following gene: 197294786

Centisome position: 89.55

GC content: 34.27

Gene sequence:

>1173_bases
GTGAATTTAAAAGAACAAGCATTACAATTACATGAAAAAAACAAAGGAAAAATTTCTGTAACAAGTAAAGTCAGAGTAGA
AAACCTATCCGATCTATCTTTAGCTTATACACCAGGAGTTGCAGAGCCTTGCTTAAAAATTAAAGAAAATCCAGAAAATG
TTTACCGTTATACTTCAAAAGGCAATATGGTAGGCGTTGTGACTAACGGAACTGCTGTTTTAGGACTGGGAGACATTGGA
CCTAAAGCTTCTTTACCAGTTATGGAAGGAAAGGCTATTCTTTTTAAAGAATTAGCAGGCGTTGATGCTTTTCCTATTTG
CATTGATTCTAAAAATACTGAAGAAATTATTAACATTGTGAAAAAAATTGCTCCTGTTTTTGGTGCAATCAATTTAGAAG
ACATTAAAGCACCTGAATGTATAGAAATTGAAGATTCCCTCAAAAAACAACTAGATATCCCTATTTTGCATGATGACCAA
CACGGAACAGCGATTGTTGCTGCTGCAGGAATTCTTAACGCTTTAAAAGTAGTGGGAAAAGCAATTGAAGATACTCAAAT
AGTTATCAGCGGAGCTGGTGCAGCCGGGATGGCGGTGGCTAAAATGTTGCTTTTGTTAAATCCTAAAAACATTATTTTAA
CCGATAAAAAAGGTGCTTTACAAAAAGACGATTTAACATTAAATAAAGAACAACATAAATTAGCACAAATAACTAATATT
TATCAAGAAAAAGGAATTTTAAAAGAAGTAATCAAAGGAAAAGATATTTTTATTGGCCTTTCTGCTCCTGGTATTTTAAC
TAGCGAAATGGTAGCCTCTATGAACAAAGATGCAATTATTTTTGCTATGGCAAATCCTACCCCAGAAATTATGCCTGATG
AAGCTAAAAAAGGTGGGGCAAGAATTATTGCAACTGGTAGATCTGATTTCCCCAACCAAGTTAATAATTGTCTAGCTTTC
CCGGGAGTTTTCCGCGGAGCTTTAGATGCTAAAGCGACTCAAATTAATGAAGAGATGAAAAAAGCCGCTGTTTACGCCCT
CAAAGGGATTATTAAAGAAGAAGATTTAAACGAAGAAAATATTTTACCAAGTAGCTTCAATAAAGAAGTAGTAAAACAAA
TTTCTTTGGCGGTAAAAGAAGCTGCTCAAAAAACTGGAGTAATTCGTAAATGA

Upstream 100 bases:

>100_bases
ATTGGCAAAAAGCAAAAAAAATATATAATAAAAGAGGATGATTGTTTTTTGCTTGCAAAAACAACCCCAAATACTTAATC
AAAAGGAGTCCACTGAAAAA

Downstream 100 bases:

>100_bases
ATGCTTCTCCAACATCGCCAGAAAAGAAAAATAACAAAAAAACCATTTTAGGATTTAGTCTTCCTGTTTTTGGCTTAATT
ATTTTACTTGTTTTAATACA

Product: Malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNLKEQALQLHEKNKGKISVTSKVRVENLSDLSLAYTPGVAEPCLKIKENPENVYRYTSKGNMVGVVTNGTAVLGLGDIG
PKASLPVMEGKAILFKELAGVDAFPICIDSKNTEEIINIVKKIAPVFGAINLEDIKAPECIEIEDSLKKQLDIPILHDDQ
HGTAIVAAAGILNALKVVGKAIEDTQIVISGAGAAGMAVAKMLLLLNPKNIILTDKKGALQKDDLTLNKEQHKLAQITNI
YQEKGILKEVIKGKDIFIGLSAPGILTSEMVASMNKDAIIFAMANPTPEIMPDEAKKGGARIIATGRSDFPNQVNNCLAF
PGVFRGALDAKATQINEEMKKAAVYALKGIIKEEDLNEENILPSSFNKEVVKQISLAVKEAAQKTGVIRK

Sequences:

>Translated_390_residues
MNLKEQALQLHEKNKGKISVTSKVRVENLSDLSLAYTPGVAEPCLKIKENPENVYRYTSKGNMVGVVTNGTAVLGLGDIG
PKASLPVMEGKAILFKELAGVDAFPICIDSKNTEEIINIVKKIAPVFGAINLEDIKAPECIEIEDSLKKQLDIPILHDDQ
HGTAIVAAAGILNALKVVGKAIEDTQIVISGAGAAGMAVAKMLLLLNPKNIILTDKKGALQKDDLTLNKEQHKLAQITNI
YQEKGILKEVIKGKDIFIGLSAPGILTSEMVASMNKDAIIFAMANPTPEIMPDEAKKGGARIIATGRSDFPNQVNNCLAF
PGVFRGALDAKATQINEEMKKAAVYALKGIIKEEDLNEENILPSSFNKEVVKQISLAVKEAAQKTGVIRK
>Mature_390_residues
MNLKEQALQLHEKNKGKISVTSKVRVENLSDLSLAYTPGVAEPCLKIKENPENVYRYTSKGNMVGVVTNGTAVLGLGDIG
PKASLPVMEGKAILFKELAGVDAFPICIDSKNTEEIINIVKKIAPVFGAINLEDIKAPECIEIEDSLKKQLDIPILHDDQ
HGTAIVAAAGILNALKVVGKAIEDTQIVISGAGAAGMAVAKMLLLLNPKNIILTDKKGALQKDDLTLNKEQHKLAQITNI
YQEKGILKEVIKGKDIFIGLSAPGILTSEMVASMNKDAIIFAMANPTPEIMPDEAKKGGARIIATGRSDFPNQVNNCLAF
PGVFRGALDAKATQINEEMKKAAVYALKGIIKEEDLNEENILPSSFNKEVVKQISLAVKEAAQKTGVIRK

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=382, Percent_Identity=26.4397905759162, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI62420880, Length=382, Percent_Identity=26.4397905759162, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI239049447, Length=382, Percent_Identity=26.4397905759162, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI4505143, Length=377, Percent_Identity=25.1989389920424, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4505145, Length=383, Percent_Identity=26.3707571801567, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788806, Length=405, Percent_Identity=46.9135802469136, Blast_Score=350, Evalue=7e-98,
Organism=Escherichia coli, GI87081919, Length=443, Percent_Identity=25.2821670428894, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537199, Length=443, Percent_Identity=24.1534988713318, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=263, Percent_Identity=30.0380228136882, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356279, Length=458, Percent_Identity=25.1091703056769, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362674, Length=458, Percent_Identity=25.1091703056769, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362672, Length=458, Percent_Identity=25.1091703056769, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24646388, Length=413, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646386, Length=413, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI78707240, Length=381, Percent_Identity=27.2965879265092, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI78707242, Length=381, Percent_Identity=27.2965879265092, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI78707238, Length=381, Percent_Identity=27.2965879265092, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281363505, Length=369, Percent_Identity=24.9322493224932, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707236, Length=369, Percent_Identity=24.9322493224932, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707232, Length=369, Percent_Identity=24.9322493224932, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281363503, Length=369, Percent_Identity=24.9322493224932, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 41907; Mature: 41907

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKEQALQLHEKNKGKISVTSKVRVENLSDLSLAYTPGVAEPCLKIKENPENVYRYTSK
CCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEECCCCCCHHHHCCCCCCHHEEECCC
GNMVGVVTNGTAVLGLGDIGPKASLPVMEGKAILFKELAGVDAFPICIDSKNTEEIINIV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHH
KKIAPVFGAINLEDIKAPECIEIEDSLKKQLDIPILHDDQHGTAIVAAAGILNALKVVGK
HHHHHHHCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
AIEDTQIVISGAGAAGMAVAKMLLLLNPKNIILTDKKGALQKDDLTLNKEQHKLAQITNI
HCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
YQEKGILKEVIKGKDIFIGLSAPGILTSEMVASMNKDAIIFAMANPTPEIMPDEAKKGGA
HHHCCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
RIIATGRSDFPNQVNNCLAFPGVFRGALDAKATQINEEMKKAAVYALKGIIKEEDLNEEN
EEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ILPSSFNKEVVKQISLAVKEAAQKTGVIRK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLKEQALQLHEKNKGKISVTSKVRVENLSDLSLAYTPGVAEPCLKIKENPENVYRYTSK
CCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEECCCCCCHHHHCCCCCCHHEEECCC
GNMVGVVTNGTAVLGLGDIGPKASLPVMEGKAILFKELAGVDAFPICIDSKNTEEIINIV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHH
KKIAPVFGAINLEDIKAPECIEIEDSLKKQLDIPILHDDQHGTAIVAAAGILNALKVVGK
HHHHHHHCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
AIEDTQIVISGAGAAGMAVAKMLLLLNPKNIILTDKKGALQKDDLTLNKEQHKLAQITNI
HCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
YQEKGILKEVIKGKDIFIGLSAPGILTSEMVASMNKDAIIFAMANPTPEIMPDEAKKGGA
HHHCCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
RIIATGRSDFPNQVNNCLAFPGVFRGALDAKATQINEEMKKAAVYALKGIIKEEDLNEEN
EEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ILPSSFNKEVVKQISLAVKEAAQKTGVIRK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]