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The map label for this gene is pdhC [H]

Identifier: 197294722

GI number: 197294722

Start: 697260

End: 698483

Strand: Direct

Name: pdhC [H]

Synonym: PAa_0688

Alternate gene names: 197294722

Gene position: 697260-698483 (Clockwise)

Preceding gene: 197294721

Following gene: 197294723

Centisome position: 79.24

GC content: 30.96

Gene sequence:

>1224_bases
ATGTTTGAATTTAAATTTGCTGATGTTGGAGAAGGTATTCATGAAGGAACCATTACAAGATGGTTTTTTAAAAAAGGCGA
TTCCGTTAAGAAAGATGATGTTTTAGTTAAAATAGAAACAGATAAATTAGATGTTGAATTAACTTCTCCTGCGACAGGAA
CTATCATTAAGATGACTCACAAAGAAGGGGATGTGATTAATGTCGGAGAAACACTAGTTTTAATTAAAGAACCAGGAGAT
TCTGAAATAGAAGTAAAAACCGAAAAAACCCCTTCATCCCACACTTCTTCTAAAGAAGAAAAAACACCTTCATTTCAACC
AAAATCTAATGACAATCAAAAAATATTGGCAACTCCTTTAGTAAGAAGTTTAGCAAAAGAATTAGGAGTTGATTTAACTA
AAGTAAAAGGGACTGGTTTTGGGGGAAAAATCTTAAAAGCAGATATCCTTTCAAATCAAAAACAAACTCAAACACCAAGC
CCTTTAATGACTCAAAGTTCTCAATTAACCTCAATGGATTCAGTTGCTCAAACAGAAGTTGTGAAAATTTCGCGTTTAAG
AAAAGCAATTGCCCAAAAAATGGTCCTTTCCAAAAGCAACATTCCAGAAACAAATTTAATGGATGAAGTAAACATCACAG
CTTTAGTTAATTTAAGAAAACAACTTAAAGAAGAAGCTGAAAAACAAGGAATTAAACTAACTTTTATGGCTTTTATTATG
AAAGCTGTTGCAATCGCTTTGAAAGAATTCCCTCTTTTTAATGCTAGTTATGATGAACCTAAAGAAGAAATTATTTTCAA
AAAATTTATTAATTTAGGAATTGCAGTTGATACTAAAGATGGTCTAATTGTTCCTAATGTTAAAAACGCTTATCCGTTAA
GTCTTTTAGAATTAGCAAAAAACTTACAAGAAGTAGTCAAAGCAACCATCGAAAGAAAAGTTCAATTAGAACAACTACAA
AATAGCACCTTTACAATTACTAATTTTGGATCTTTAGATATTAGTTATGGAACCCCTGTAATTAATTATCCTGAAGTAGC
AATTTTGGGTGTTGGAAAAATCTCCAAAAAACCAATTGTAGAAAATAATCAAATTGTTGTTGCTGATATGTTGCCGCTTT
CTCTTGCAATCGACCACCGTATTATCGATGGAGCTGATGGAGGAAGATTCTTAAAACGAATTAAAGAATTATTAAAATCA
CCTACTTTATTATTTTTATCCTAA

Upstream 100 bases:

>100_bases
TAGCCAAAGGAGAACATTATCAATTTTTAAGTCCTGAAAAAATAGCTGCTGCTATTCGCAAAGTAGCTTTAGAAGAATAA
ACATAACAAGGAGAAAAAAT

Downstream 100 bases:

>100_bases
ATGAAGAAAGTATAACATAAATATGAAAAATTATGATATTTTAATAATCGGTGGAGGACCGGGAGGATATGTGGCCGCAA
TCAAGGCGTCCCAATTAGGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MFEFKFADVGEGIHEGTITRWFFKKGDSVKKDDVLVKIETDKLDVELTSPATGTIIKMTHKEGDVINVGETLVLIKEPGD
SEIEVKTEKTPSSHTSSKEEKTPSFQPKSNDNQKILATPLVRSLAKELGVDLTKVKGTGFGGKILKADILSNQKQTQTPS
PLMTQSSQLTSMDSVAQTEVVKISRLRKAIAQKMVLSKSNIPETNLMDEVNITALVNLRKQLKEEAEKQGIKLTFMAFIM
KAVAIALKEFPLFNASYDEPKEEIIFKKFINLGIAVDTKDGLIVPNVKNAYPLSLLELAKNLQEVVKATIERKVQLEQLQ
NSTFTITNFGSLDISYGTPVINYPEVAILGVGKISKKPIVENNQIVVADMLPLSLAIDHRIIDGADGGRFLKRIKELLKS
PTLLFLS

Sequences:

>Translated_407_residues
MFEFKFADVGEGIHEGTITRWFFKKGDSVKKDDVLVKIETDKLDVELTSPATGTIIKMTHKEGDVINVGETLVLIKEPGD
SEIEVKTEKTPSSHTSSKEEKTPSFQPKSNDNQKILATPLVRSLAKELGVDLTKVKGTGFGGKILKADILSNQKQTQTPS
PLMTQSSQLTSMDSVAQTEVVKISRLRKAIAQKMVLSKSNIPETNLMDEVNITALVNLRKQLKEEAEKQGIKLTFMAFIM
KAVAIALKEFPLFNASYDEPKEEIIFKKFINLGIAVDTKDGLIVPNVKNAYPLSLLELAKNLQEVVKATIERKVQLEQLQ
NSTFTITNFGSLDISYGTPVINYPEVAILGVGKISKKPIVENNQIVVADMLPLSLAIDHRIIDGADGGRFLKRIKELLKS
PTLLFLS
>Mature_407_residues
MFEFKFADVGEGIHEGTITRWFFKKGDSVKKDDVLVKIETDKLDVELTSPATGTIIKMTHKEGDVINVGETLVLIKEPGD
SEIEVKTEKTPSSHTSSKEEKTPSFQPKSNDNQKILATPLVRSLAKELGVDLTKVKGTGFGGKILKADILSNQKQTQTPS
PLMTQSSQLTSMDSVAQTEVVKISRLRKAIAQKMVLSKSNIPETNLMDEVNITALVNLRKQLKEEAEKQGIKLTFMAFIM
KAVAIALKEFPLFNASYDEPKEEIIFKKFINLGIAVDTKDGLIVPNVKNAYPLSLLELAKNLQEVVKATIERKVQLEQLQ
NSTFTITNFGSLDISYGTPVINYPEVAILGVGKISKKPIVENNQIVVADMLPLSLAIDHRIIDGADGGRFLKRIKELLKS
PTLLFLS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=35.7476635514019, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI31711992, Length=421, Percent_Identity=29.6912114014252, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=31.4159292035398, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=26, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=26, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI260898739, Length=165, Percent_Identity=29.6969696969697, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786946, Length=401, Percent_Identity=32.1695760598504, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786305, Length=408, Percent_Identity=32.5980392156863, Blast_Score=211, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17537937, Length=415, Percent_Identity=31.566265060241, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=28.5377358490566, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=426, Percent_Identity=28.6384976525822, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=28.1045751633987, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=404, Percent_Identity=30.9405940594059, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324258, Length=437, Percent_Identity=27.0022883295195, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=33.9622641509434, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=31.5555555555556, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24582497, Length=298, Percent_Identity=25.503355704698, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20129315, Length=298, Percent_Identity=25.503355704698, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44980; Mature: 44980

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEFKFADVGEGIHEGTITRWFFKKGDSVKKDDVLVKIETDKLDVELTSPATGTIIKMTH
CCEEEEHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEEEEECCCCCCEEEEEEC
KEGDVINVGETLVLIKEPGDSEIEVKTEKTPSSHTSSKEEKTPSFQPKSNDNQKILATPL
CCCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCEEEHHHH
VRSLAKELGVDLTKVKGTGFGGKILKADILSNQKQTQTPSPLMTQSSQLTSMDSVAQTEV
HHHHHHHHCCEEEEEECCCCCCCEEEHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHH
VKISRLRKAIAQKMVLSKSNIPETNLMDEVNITALVNLRKQLKEEAEKQGIKLTFMAFIM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
KAVAIALKEFPLFNASYDEPKEEIIFKKFINLGIAVDTKDGLIVPNVKNAYPLSLLELAK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCCHHHHHHHH
NLQEVVKATIERKVQLEQLQNSTFTITNFGSLDISYGTPVINYPEVAILGVGKISKKPIV
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCCCCC
ENNQIVVADMLPLSLAIDHRIIDGADGGRFLKRIKELLKSPTLLFLS
CCCCEEEEECCCEEEEECCEEECCCCCHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MFEFKFADVGEGIHEGTITRWFFKKGDSVKKDDVLVKIETDKLDVELTSPATGTIIKMTH
CCEEEEHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEEEEECCCCCCEEEEEEC
KEGDVINVGETLVLIKEPGDSEIEVKTEKTPSSHTSSKEEKTPSFQPKSNDNQKILATPL
CCCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCEEEHHHH
VRSLAKELGVDLTKVKGTGFGGKILKADILSNQKQTQTPSPLMTQSSQLTSMDSVAQTEV
HHHHHHHHCCEEEEEECCCCCCCEEEHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHH
VKISRLRKAIAQKMVLSKSNIPETNLMDEVNITALVNLRKQLKEEAEKQGIKLTFMAFIM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
KAVAIALKEFPLFNASYDEPKEEIIFKKFINLGIAVDTKDGLIVPNVKNAYPLSLLELAK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCCHHHHHHHH
NLQEVVKATIERKVQLEQLQNSTFTITNFGSLDISYGTPVINYPEVAILGVGKISKKPIV
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCCCCC
ENNQIVVADMLPLSLAIDHRIIDGADGGRFLKRIKELLKSPTLLFLS
CCCCEEEEECCCEEEEECCEEECCCCCHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1735725 [H]