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The map label for this gene is pdhD [H]

Identifier: 197294723

GI number: 197294723

Start: 698506

End: 699879

Strand: Direct

Name: pdhD [H]

Synonym: PAa_0689

Alternate gene names: 197294723

Gene position: 698506-699879 (Clockwise)

Preceding gene: 197294722

Following gene: 197294724

Centisome position: 79.38

GC content: 32.31

Gene sequence:

>1374_bases
ATGAAAAATTATGATATTTTAATAATCGGTGGAGGACCGGGAGGATATGTGGCCGCAATCAAGGCGTCCCAATTAGGGGC
TAAAGTTGCTTTAGTTGAAGATCATAAATTAGGCGGCATTTGTCTTAATTATGGATGCATTCCTACCAAAACTTATTTAA
AAAGTGCCAAAGTTTATCAAACTATCCAACATGCTCAAGATTTTGGCATTACACTTAATCAACCGCCCACTTTTAATTGG
CTTGCTATTTTTAATCGTAAAAATAAAATTGTCAATCAATTAACTAGTGGCATCGCTTTTTTACTTAAAAAAAATAAAGT
TGATGTTTATAATGGGTTTGCAGTTCCTCTTTCTCCTCAAAAAATCCAAGTTAATAAAGAAATATTAGAAACTGAAAAAC
TTATTATTGCAACAGGCGCTACCGCCTTTATTCCTCCAATTCCAGGAGCTTTAGAAGCTTATCAAAAAAATATTTTAAAA
ACTAGCAAAGAATTATTGCAATTAGACAAACATCCCAAAAATATTATTATTATCGGCGGCGGAGTAATTGGGGTTGAATT
TGCTACCATTCATAAATCTTTTGGTGCTGAAGTTACTATTTTAGAAAGACAAAGCAATATTTTAAATGGTTCAGATCATG
ATATTGTTAACGCTTACACTAAAAGGTTAAAAAGTGATGGAATTAATGTTTTAACAGAAGTTCAAATCAATTCAATTCAA
GGTCATAAAGTTACCTATACTCACCAAAATATTCAAACCACTCAAGAAGCAGAAGTTATTTTAATGGCTGCAGGAACAAA
ACCTAATTTGGCTGGTTTAGAAAAACTTGATTTAGAAAAAAATAACAATAGTATTGTTACTGATGAATTTTTACAAACTT
CTATTCCTGGAGTTTATGCAATCGGTGATGTTAACGGTAAGTATATGTTGGCTCACGTAGCTAGTCATGAAGGAATTATT
GCCGTAATGCATGCTTTAGGCAAAGGAGAACATGGTATAAATTATAATCGTATTCCTTCTTGTATTTATGGCTTTCCTGA
AATTGCTTCTATCGGAATGACCGAACAAGATGCCCAAATGAAAAAAATAGATTATAAAGTTTCTAAAGTTCCACTTTCTG
CCATCGGAAAAGCTTTAGCTGATGGTGAAAAAGAAGGATTTGCTAAAATTATTGTTGATAAAAAACATTTAGAGATTATT
GGTATGCATATTTATGCTTATAATGCAACTGAATTAATTAGCGAAATTGCTGTTGGAATGGAATTAGAAGGAACAGCATA
TGAATTGGCCCAAGCAATTCATCCTCATCCAACATTATCAGAATTAACTTTTGAAGCTCTTTTAGGTGCTATCGACAAAC
CTATTCACGTTTAA

Upstream 100 bases:

>100_bases
GATGGAGCTGATGGAGGAAGATTCTTAAAACGAATTAAAGAATTATTAAAATCACCTACTTTATTATTTTTATCCTAAAT
GAAGAAAGTATAACATAAAT

Downstream 100 bases:

>100_bases
AAATGAAGACAAATAAAAACTAACTTTTTTTAAGTTAGCTTTTTTTATTTTATTCAAAATCCATAAATTAATTTATTCTT
TTGTCCAATTTAGTCAAAAT

Product: Dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKNYDILIIGGGPGGYVAAIKASQLGAKVALVEDHKLGGICLNYGCIPTKTYLKSAKVYQTIQHAQDFGITLNQPPTFNW
LAIFNRKNKIVNQLTSGIAFLLKKNKVDVYNGFAVPLSPQKIQVNKEILETEKLIIATGATAFIPPIPGALEAYQKNILK
TSKELLQLDKHPKNIIIIGGGVIGVEFATIHKSFGAEVTILERQSNILNGSDHDIVNAYTKRLKSDGINVLTEVQINSIQ
GHKVTYTHQNIQTTQEAEVILMAAGTKPNLAGLEKLDLEKNNNSIVTDEFLQTSIPGVYAIGDVNGKYMLAHVASHEGII
AVMHALGKGEHGINYNRIPSCIYGFPEIASIGMTEQDAQMKKIDYKVSKVPLSAIGKALADGEKEGFAKIIVDKKHLEII
GMHIYAYNATELISEIAVGMELEGTAYELAQAIHPHPTLSELTFEALLGAIDKPIHV

Sequences:

>Translated_457_residues
MKNYDILIIGGGPGGYVAAIKASQLGAKVALVEDHKLGGICLNYGCIPTKTYLKSAKVYQTIQHAQDFGITLNQPPTFNW
LAIFNRKNKIVNQLTSGIAFLLKKNKVDVYNGFAVPLSPQKIQVNKEILETEKLIIATGATAFIPPIPGALEAYQKNILK
TSKELLQLDKHPKNIIIIGGGVIGVEFATIHKSFGAEVTILERQSNILNGSDHDIVNAYTKRLKSDGINVLTEVQINSIQ
GHKVTYTHQNIQTTQEAEVILMAAGTKPNLAGLEKLDLEKNNNSIVTDEFLQTSIPGVYAIGDVNGKYMLAHVASHEGII
AVMHALGKGEHGINYNRIPSCIYGFPEIASIGMTEQDAQMKKIDYKVSKVPLSAIGKALADGEKEGFAKIIVDKKHLEII
GMHIYAYNATELISEIAVGMELEGTAYELAQAIHPHPTLSELTFEALLGAIDKPIHV
>Mature_457_residues
MKNYDILIIGGGPGGYVAAIKASQLGAKVALVEDHKLGGICLNYGCIPTKTYLKSAKVYQTIQHAQDFGITLNQPPTFNW
LAIFNRKNKIVNQLTSGIAFLLKKNKVDVYNGFAVPLSPQKIQVNKEILETEKLIIATGATAFIPPIPGALEAYQKNILK
TSKELLQLDKHPKNIIIIGGGVIGVEFATIHKSFGAEVTILERQSNILNGSDHDIVNAYTKRLKSDGINVLTEVQINSIQ
GHKVTYTHQNIQTTQEAEVILMAAGTKPNLAGLEKLDLEKNNNSIVTDEFLQTSIPGVYAIGDVNGKYMLAHVASHEGII
AVMHALGKGEHGINYNRIPSCIYGFPEIASIGMTEQDAQMKKIDYKVSKVPLSAIGKALADGEKEGFAKIIVDKKHLEII
GMHIYAYNATELISEIAVGMELEGTAYELAQAIHPHPTLSELTFEALLGAIDKPIHV

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=34.7547974413646, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=29.0672451193059, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI22035672, Length=475, Percent_Identity=26.7368421052632, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277065, Length=458, Percent_Identity=24.8908296943231, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI33519430, Length=458, Percent_Identity=24.8908296943231, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI33519428, Length=458, Percent_Identity=24.8908296943231, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI33519426, Length=458, Percent_Identity=24.8908296943231, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI148277071, Length=458, Percent_Identity=24.8908296943231, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=458, Percent_Identity=26.4192139737991, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045268, Length=456, Percent_Identity=26.0964912280702, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=33.2613390928726, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=29.5652173913043, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082354, Length=474, Percent_Identity=27.4261603375527, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=25.9174311926606, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789065, Length=187, Percent_Identity=28.8770053475936, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788892, Length=217, Percent_Identity=27.6497695852535, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=467, Percent_Identity=36.4025695931477, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=26.963906581741, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71983429, Length=463, Percent_Identity=26.133909287257, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=463, Percent_Identity=26.133909287257, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=479, Percent_Identity=27.7661795407098, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=39.375, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.7257019438445, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=29.2372881355932, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=35.8050847457627, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24640549, Length=380, Percent_Identity=25.7894736842105, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640553, Length=380, Percent_Identity=25.7894736842105, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=380, Percent_Identity=25.7894736842105, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=22.4318658280922, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49691; Mature: 49691

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYDILIIGGGPGGYVAAIKASQLGAKVALVEDHKLGGICLNYGCIPTKTYLKSAKVYQ
CCCEEEEEEECCCCCEEEEEEEHHCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHH
TIQHAQDFGITLNQPPTFNWLAIFNRKNKIVNQLTSGIAFLLKKNKVDVYNGFAVPLSPQ
HHHHHHHCCEEECCCCCEEEEEEECCHHHHHHHHHHHHEEEHCCCCEEEECCEEECCCCC
KIQVNKEILETEKLIIATGATAFIPPIPGALEAYQKNILKTSKELLQLDKHPKNIIIIGG
EEECCHHHHCCCEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GVIGVEFATIHKSFGAEVTILERQSNILNGSDHDIVNAYTKRLKSDGINVLTEVQINSIQ
CEEEEEEEEHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCC
GHKVTYTHQNIQTTQEAEVILMAAGTKPNLAGLEKLDLEKNNNSIVTDEFLQTSIPGVYA
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEHHHHHHCCCCEEE
IGDVNGKYMLAHVASHEGIIAVMHALGKGEHGINYNRIPSCIYGFPEIASIGMTEQDAQM
EECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHCCCCCCCCHH
KKIDYKVSKVPLSAIGKALADGEKEGFAKIIVDKKHLEIIGMHIYAYNATELISEIAVGM
HHHCCHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEEEEECHHHHHHHHHCCE
ELEGTAYELAQAIHPHPTLSELTFEALLGAIDKPIHV
ECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNYDILIIGGGPGGYVAAIKASQLGAKVALVEDHKLGGICLNYGCIPTKTYLKSAKVYQ
CCCEEEEEEECCCCCEEEEEEEHHCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHH
TIQHAQDFGITLNQPPTFNWLAIFNRKNKIVNQLTSGIAFLLKKNKVDVYNGFAVPLSPQ
HHHHHHHCCEEECCCCCEEEEEEECCHHHHHHHHHHHHEEEHCCCCEEEECCEEECCCCC
KIQVNKEILETEKLIIATGATAFIPPIPGALEAYQKNILKTSKELLQLDKHPKNIIIIGG
EEECCHHHHCCCEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GVIGVEFATIHKSFGAEVTILERQSNILNGSDHDIVNAYTKRLKSDGINVLTEVQINSIQ
CEEEEEEEEHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCC
GHKVTYTHQNIQTTQEAEVILMAAGTKPNLAGLEKLDLEKNNNSIVTDEFLQTSIPGVYA
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEHHHHHHCCCCEEE
IGDVNGKYMLAHVASHEGIIAVMHALGKGEHGINYNRIPSCIYGFPEIASIGMTEQDAQM
EECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHCCCCCCCCHH
KKIDYKVSKVPLSAIGKALADGEKEGFAKIIVDKKHLEIIGMHIYAYNATELISEIAVGM
HHHCCHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEEEEECHHHHHHHHHCCE
ELEGTAYELAQAIHPHPTLSELTFEALLGAIDKPIHV
ECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1735725 [H]