The gene/protein map for NC_010544 is currently unavailable.
Definition Candidatus Phytoplasma australiense, complete genome.
Accession NC_010544
Length 879,959

Click here to switch to the map view.

The map label for this gene is proS

Identifier: 197294712

GI number: 197294712

Start: 686172

End: 687596

Strand: Reverse

Name: proS

Synonym: PAa_0678

Alternate gene names: 197294712

Gene position: 687596-686172 (Counterclockwise)

Preceding gene: 197294713

Following gene: 197294711

Centisome position: 78.14

GC content: 32.49

Gene sequence:

>1425_bases
ATGAAATCAATTAAACGAGTAGAAAAAGTCACTTCACACTTATCTGATTTTGGTCAATGGTATACAGATATTTGCTTAAA
GGCTGAATTAATAGCGTATTCAGATGTTAAAGGATTTATTATTTATTTGCCTTATGGTTATGCCTTGTGGGAAAATATTC
AAAAATATCTTAATTATGAATTCCAAAAAACAGGACACCAAAATGTGTATTTTCCTTTAGTGTTTTCTGAAAAGCTTTTT
CAAAAAGAAAAAGACCATATTCAAGGTTTTTCACCCGAAGCGGCTATGATTACTTCTACTGGTCAAAAAGAATTATTTGA
AAAACTTGTTATCCGTCCGACTTCAGAAGTTTTATTTTCGCAATATTACGCCAAAATTATTAGTTCTTACCGTGATTTGC
CTAAGTTATATAATCAATTTTGCAACGTTGTTCGTTGGGAAAAAGCAACCAAACCTTTTTTACGTGGCAAAGAATTTTTA
TGGCAAGAAGGTCATACTGTTCATGCTACTGAAAAAGAAGCACTTGATCAAACTTTGTTTGCGATTCAACTTTACGAAAA
ATTAGGCAAGGAACTTTTAGCCCTTCCTTTTGTTTGTGGTCGCAAAACTGAAAAGGAAAAATTTGCCGGTGCTTTAATTA
CTTATTCTATTGAAGCTTTAATGCAAGATGGTCAAGCTTTACAAGCAGGAACCTCTCATTATTTAGGCACTAATTTCGCT
CAATCATTTCAAATTCAATTTCAAGATCAAGATCATCAAAATAAATATGTTCATCAAACTTCTTGGGGTGTTTCAACACG
ACTAATTGGAGCTTTAATTATGGTTCACAGTGATGATGAAGGCTTAATTTTGCCTCCTTATATCGCTCCCATTCAAATTG
TTATTATCCCTTTACAACCTAAAAATGAATTGGTTCAAGCAGAAGCTAAAAAAATCTTTGATGACTTAAAAACAACTTAT
CGTGTTCATTTGGATTTACAAAATAAAACCCCTGGTTGGAAATTTAGTCAATACGAACTTAAAGGAGTCCCTTTGAGGAT
TGAAATTGGCGCGCGTGATTTAGCCCAAGGAGAAGTAACTATTTTCCAAAGGTATAATAATTCTAAGAATAATTTTTCCA
AAAACACTTTCAAAGCAGAAATCCCTAAATTATTAAAAACTATTCATGATAAAATGTATCAAAAAGCCCAAAAACATTTA
GAGGCCAACCGAAGACAAGCAAGCACTTATCAAGAATTTAAAGATCATCTGCAAAAAAAAGGTTATGTGGCGATGAGTAT
TTCAGGTATGGATGCTGAAATAAAAATCAAAACAGAAACCGGAGCAACCGCAAGAGTTATTTTAGAGACTCCTTTAATCA
CTCCTAATTGTCCTGTTACCGACAAAAAAGCTATTCAAACAGTTTTATTTGCTAAATCTTATTAA

Upstream 100 bases:

>100_bases
ATTTTTTTTATTTCACATCGGATACATGCTTTTAAAAAGCGATAATAACACCAAGGAATAATTTTTTCCTTCAAAATAAC
TATATAAAGGTTATAGAAAA

Downstream 100 bases:

>100_bases
AATTTTTTTATCTTGTTTTTATTTTTTAAACATTTTATCTTATTTTTTTTAAGCTTTAACTATTCTAAAAAAAACTGCAT
TAAATAAATAGAAAGAGATT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKSIKRVEKVTSHLSDFGQWYTDICLKAELIAYSDVKGFIIYLPYGYALWENIQKYLNYEFQKTGHQNVYFPLVFSEKLF
QKEKDHIQGFSPEAAMITSTGQKELFEKLVIRPTSEVLFSQYYAKIISSYRDLPKLYNQFCNVVRWEKATKPFLRGKEFL
WQEGHTVHATEKEALDQTLFAIQLYEKLGKELLALPFVCGRKTEKEKFAGALITYSIEALMQDGQALQAGTSHYLGTNFA
QSFQIQFQDQDHQNKYVHQTSWGVSTRLIGALIMVHSDDEGLILPPYIAPIQIVIIPLQPKNELVQAEAKKIFDDLKTTY
RVHLDLQNKTPGWKFSQYELKGVPLRIEIGARDLAQGEVTIFQRYNNSKNNFSKNTFKAEIPKLLKTIHDKMYQKAQKHL
EANRRQASTYQEFKDHLQKKGYVAMSISGMDAEIKIKTETGATARVILETPLITPNCPVTDKKAIQTVLFAKSY

Sequences:

>Translated_474_residues
MKSIKRVEKVTSHLSDFGQWYTDICLKAELIAYSDVKGFIIYLPYGYALWENIQKYLNYEFQKTGHQNVYFPLVFSEKLF
QKEKDHIQGFSPEAAMITSTGQKELFEKLVIRPTSEVLFSQYYAKIISSYRDLPKLYNQFCNVVRWEKATKPFLRGKEFL
WQEGHTVHATEKEALDQTLFAIQLYEKLGKELLALPFVCGRKTEKEKFAGALITYSIEALMQDGQALQAGTSHYLGTNFA
QSFQIQFQDQDHQNKYVHQTSWGVSTRLIGALIMVHSDDEGLILPPYIAPIQIVIIPLQPKNELVQAEAKKIFDDLKTTY
RVHLDLQNKTPGWKFSQYELKGVPLRIEIGARDLAQGEVTIFQRYNNSKNNFSKNTFKAEIPKLLKTIHDKMYQKAQKHL
EANRRQASTYQEFKDHLQKKGYVAMSISGMDAEIKIKTETGATARVILETPLITPNCPVTDKKAIQTVLFAKSY
>Mature_474_residues
MKSIKRVEKVTSHLSDFGQWYTDICLKAELIAYSDVKGFIIYLPYGYALWENIQKYLNYEFQKTGHQNVYFPLVFSEKLF
QKEKDHIQGFSPEAAMITSTGQKELFEKLVIRPTSEVLFSQYYAKIISSYRDLPKLYNQFCNVVRWEKATKPFLRGKEFL
WQEGHTVHATEKEALDQTLFAIQLYEKLGKELLALPFVCGRKTEKEKFAGALITYSIEALMQDGQALQAGTSHYLGTNFA
QSFQIQFQDQDHQNKYVHQTSWGVSTRLIGALIMVHSDDEGLILPPYIAPIQIVIIPLQPKNELVQAEAKKIFDDLKTTY
RVHLDLQNKTPGWKFSQYELKGVPLRIEIGARDLAQGEVTIFQRYNNSKNNFSKNTFKAEIPKLLKTIHDKMYQKAQKHL
EANRRQASTYQEFKDHLQKKGYVAMSISGMDAEIKIKTETGATARVILETPLITPNCPVTDKKAIQTVLFAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=440, Percent_Identity=41.1363636363636, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI1786392, Length=148, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71991342, Length=484, Percent_Identity=39.0495867768595, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI71991346, Length=359, Percent_Identity=42.8969359331476, Blast_Score=320, Evalue=7e-88,
Organism=Saccharomyces cerevisiae, GI6321808, Length=495, Percent_Identity=38.7878787878788, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24649468, Length=439, Percent_Identity=40.0911161731207, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24649466, Length=439, Percent_Identity=40.0911161731207, Blast_Score=348, Evalue=5e-96,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PHYAS (B1VAN9)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799253.1
- ProteinModelPortal:   B1VAN9
- SMR:   B1VAN9
- GeneID:   6799635
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54603; Mature: 54603

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKSIKRVEKVTSHLSDFGQWYTDICLKAELIAYSDVKGFIIYLPYGYALWENIQKYLNYE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHCCH
FQKTGHQNVYFPLVFSEKLFQKEKDHIQGFSPEAAMITSTGQKELFEKLVIRPTSEVLFS
HHCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCHHHHHHH
QYYAKIISSYRDLPKLYNQFCNVVRWEKATKPFLRGKEFLWQEGHTVHATEKEALDQTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCEEECCHHHHHHHHHH
AIQLYEKLGKELLALPFVCGRKTEKEKFAGALITYSIEALMQDGQALQAGTSHYLGTNFA
HHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHCCCCCC
QSFQIQFQDQDHQNKYVHQTSWGVSTRLIGALIMVHSDDEGLILPPYIAPIQIVIIPLQP
CEEEEEECCCCCCCCEEEECCCCHHHHHHHEEEEEEECCCCEEECCCCCCEEEEEEECCC
KNELVQAEAKKIFDDLKTTYRVHLDLQNKTPGWKFSQYELKGVPLRIEIGARDLAQGEVT
CHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCEE
IFQRYNNSKNNFSKNTFKAEIPKLLKTIHDKMYQKAQKHLEANRRQASTYQEFKDHLQKK
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYVAMSISGMDAEIKIKTETGATARVILETPLITPNCPVTDKKAIQTVLFAKSY
CEEEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKSIKRVEKVTSHLSDFGQWYTDICLKAELIAYSDVKGFIIYLPYGYALWENIQKYLNYE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHCCH
FQKTGHQNVYFPLVFSEKLFQKEKDHIQGFSPEAAMITSTGQKELFEKLVIRPTSEVLFS
HHCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCHHHHHHH
QYYAKIISSYRDLPKLYNQFCNVVRWEKATKPFLRGKEFLWQEGHTVHATEKEALDQTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCEEECCHHHHHHHHHH
AIQLYEKLGKELLALPFVCGRKTEKEKFAGALITYSIEALMQDGQALQAGTSHYLGTNFA
HHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHCCCCCC
QSFQIQFQDQDHQNKYVHQTSWGVSTRLIGALIMVHSDDEGLILPPYIAPIQIVIIPLQP
CEEEEEECCCCCCCCEEEECCCCHHHHHHHEEEEEEECCCCEEECCCCCCEEEEEEECCC
KNELVQAEAKKIFDDLKTTYRVHLDLQNKTPGWKFSQYELKGVPLRIEIGARDLAQGEVT
CHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCEE
IFQRYNNSKNNFSKNTFKAEIPKLLKTIHDKMYQKAQKHLEANRRQASTYQEFKDHLQKK
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYVAMSISGMDAEIKIKTETGATARVILETPLITPNCPVTDKKAIQTVLFAKSY
CEEEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA