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The map label for this gene is lplA [H]

Identifier: 197294581

GI number: 197294581

Start: 537120

End: 538130

Strand: Direct

Name: lplA [H]

Synonym: PAa_0537

Alternate gene names: 197294581

Gene position: 537120-538130 (Clockwise)

Preceding gene: 197294569

Following gene: 197294582

Centisome position: 61.04

GC content: 29.48

Gene sequence:

>1011_bases
ATGATTTTAATCGAAACTAATCGCCCTTGCGATTTAAAACCTTATTTTTACTATGCTTTAGAAGCTTATGTTCTGAATCA
TTTATTAAAACAAAACCCAACAGAAAGTTATTTTTTTCTTTGGAAAATTAAAGGAGTTGTTGTTGGCAAAAACCAAATTA
TTGAAAATGAAGTTAACTTAGATTACCTTCAAAAAAATAAAATTGCTTTATTTAGAAGGCCTACCGGCGGGGGTTGTGTT
TATAACGACCCAAAAACCCCACTTTATAGTATTATTACCAAAAAAGAGAAAAATTTTAGTTTTAAACCTTATTTATCTAA
AATAGTTCTTGCGTTACAAAACTTAGGATTAAAAGTGGTTTTTAGTGGAAGAAACGATATTTTATTGGAAGGTAAAAAAA
TTTCTGGTAACGCTTTTTTGCAAAATCAAAATGGAATGGTCACTCACGGCACTTTGCTTTATGATTGCGATTTTAATACC
ATGATTAATGCAATTACTCCAAACGATCAAAAACTTATTTCTAAAGGAATTGCAAGCGTTCGTTCGAGAGTTGTCAATCT
AAAAAAATACCTTCCCCAAGAAATGTCTCAAGAAGCTTTTATGAAATACCTTTCTCAATCTTTAACTGTTAAGACTTATT
CATTAAATCCGGAAGAAATCAAAACAATCGAAAAAGATGCCGTAAAATATAGTTTACCTGATTTTTTATTTTTAGAACAA
CCCGAACACACCAAAATCCTTAAAAGACGATTTTCATGGGGTATGTTAGAAATTTTTTTAAAGATTAGATTTGGCAGAAT
TCAAAATTTAGCTTTAATCGGAGATTTCTTTCACAAAGAAGATACCCTCAAAGAGTTTTGTCAGGCTTTTGAAGGAGTTA
GCTACCAAGTCAAACATTTAAAAAAAGTTTTGTTACAAAACGACATTAATCTTTATATTCTTGATGCTACTAATGATGAT
TTTTTAAACTTATTAAAGGAAGGGTTTTTAGCAGAAAAACTTAGTTATTAG

Upstream 100 bases:

>100_bases
TTTTTTAAAGACTATATTTAAACTATTTTTTTGAACTTAACTCCGACTACTTTTTAAAAGAAAAAAATAAATGTTATAAT
ATAATTGAAAGAGGTATTTT

Downstream 100 bases:

>100_bases
TTTTTTGCTAAAAATAAAGGAATATTTTTATGTTAAATGATCAAAAAAAAACTTTACAAAAAATTATTATAACTTCGTTT
TTATTATTTTTAACTTTTTC

Product: Lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MILIETNRPCDLKPYFYYALEAYVLNHLLKQNPTESYFFLWKIKGVVVGKNQIIENEVNLDYLQKNKIALFRRPTGGGCV
YNDPKTPLYSIITKKEKNFSFKPYLSKIVLALQNLGLKVVFSGRNDILLEGKKISGNAFLQNQNGMVTHGTLLYDCDFNT
MINAITPNDQKLISKGIASVRSRVVNLKKYLPQEMSQEAFMKYLSQSLTVKTYSLNPEEIKTIEKDAVKYSLPDFLFLEQ
PEHTKILKRRFSWGMLEIFLKIRFGRIQNLALIGDFFHKEDTLKEFCQAFEGVSYQVKHLKKVLLQNDINLYILDATNDD
FLNLLKEGFLAEKLSY

Sequences:

>Translated_336_residues
MILIETNRPCDLKPYFYYALEAYVLNHLLKQNPTESYFFLWKIKGVVVGKNQIIENEVNLDYLQKNKIALFRRPTGGGCV
YNDPKTPLYSIITKKEKNFSFKPYLSKIVLALQNLGLKVVFSGRNDILLEGKKISGNAFLQNQNGMVTHGTLLYDCDFNT
MINAITPNDQKLISKGIASVRSRVVNLKKYLPQEMSQEAFMKYLSQSLTVKTYSLNPEEIKTIEKDAVKYSLPDFLFLEQ
PEHTKILKRRFSWGMLEIFLKIRFGRIQNLALIGDFFHKEDTLKEFCQAFEGVSYQVKHLKKVLLQNDINLYILDATNDD
FLNLLKEGFLAEKLSY
>Mature_336_residues
MILIETNRPCDLKPYFYYALEAYVLNHLLKQNPTESYFFLWKIKGVVVGKNQIIENEVNLDYLQKNKIALFRRPTGGGCV
YNDPKTPLYSIITKKEKNFSFKPYLSKIVLALQNLGLKVVFSGRNDILLEGKKISGNAFLQNQNGMVTHGTLLYDCDFNT
MINAITPNDQKLISKGIASVRSRVVNLKKYLPQEMSQEAFMKYLSQSLTVKTYSLNPEEIKTIEKDAVKYSLPDFLFLEQ
PEHTKILKRRFSWGMLEIFLKIRFGRIQNLALIGDFFHKEDTLKEFCQAFEGVSYQVKHLKKVLLQNDINLYILDATNDD
FLNLLKEGFLAEKLSY

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Escherichia coli, GI1790846, Length=304, Percent_Identity=29.2763157894737, Blast_Score=128, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6322415, Length=243, Percent_Identity=30.0411522633745, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38920; Mature: 38920

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIETNRPCDLKPYFYYALEAYVLNHLLKQNPTESYFFLWKIKGVVVGKNQIIENEVNL
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEECCCCCC
DYLQKNKIALFRRPTGGGCVYNDPKTPLYSIITKKEKNFSFKPYLSKIVLALQNLGLKVV
CEEECCCEEEEECCCCCCCEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEE
FSGRNDILLEGKKISGNAFLQNQNGMVTHGTLLYDCDFNTMINAITPNDQKLISKGIASV
ECCCCCEEEECCEECCCEEEECCCCEEEECEEEEECCHHHEEEECCCCHHHHHHHHHHHH
RSRVVNLKKYLPQEMSQEAFMKYLSQSLTVKTYSLNPEEIKTIEKDAVKYSLPDFLFLEQ
HHHHHHHHHHCHHHCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECC
PEHTKILKRRFSWGMLEIFLKIRFGRIQNLALIGDFFHKEDTLKEFCQAFEGVSYQVKHL
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
KKVLLQNDINLYILDATNDDFLNLLKEGFLAEKLSY
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILIETNRPCDLKPYFYYALEAYVLNHLLKQNPTESYFFLWKIKGVVVGKNQIIENEVNL
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEECCCCCC
DYLQKNKIALFRRPTGGGCVYNDPKTPLYSIITKKEKNFSFKPYLSKIVLALQNLGLKVV
CEEECCCEEEEECCCCCCCEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEE
FSGRNDILLEGKKISGNAFLQNQNGMVTHGTLLYDCDFNTMINAITPNDQKLISKGIASV
ECCCCCEEEECCEECCCEEEECCCCEEEECEEEEECCHHHEEEECCCCHHHHHHHHHHHH
RSRVVNLKKYLPQEMSQEAFMKYLSQSLTVKTYSLNPEEIKTIEKDAVKYSLPDFLFLEQ
HHHHHHHHHHCHHHCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECC
PEHTKILKRRFSWGMLEIFLKIRFGRIQNLALIGDFFHKEDTLKEFCQAFEGVSYQVKHL
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
KKVLLQNDINLYILDATNDDFLNLLKEGFLAEKLSY
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]