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The map label for this gene is mesJ [H]

Identifier: 197294532

GI number: 197294532

Start: 484147

End: 485454

Strand: Direct

Name: mesJ [H]

Synonym: PAa_0487

Alternate gene names: 197294532

Gene position: 484147-485454 (Clockwise)

Preceding gene: 197294531

Following gene: 197294533

Centisome position: 55.02

GC content: 25.84

Gene sequence:

>1308_bases
ATGAAACTAGCTTTAAAATTAGATTTTCAAAAAACTTATATTATAGCTGTTAGCGGCGGCGTTGATTCGATGGTTTTATT
AGATTTTTTGTGGTCACAAAAATACAAGTTAAAAGTAGTTCATTTCAATCATTTAAAACGTTTGAATGCAATCAAAGATA
AAAATTTAATTCAATCTTATTGCGATCAAAGAAAAATCCCTTTTTATTATTTTGAATTACAACTTAATCCTCAAAATTTT
CAAACTGAAGCTCGTTTGTTAAGGCAAGAAAAACTGAAACAAATTGCTAAAAAATACCAAACTCCTTATGTAATAACAGC
TCATCATTTAGATGATTTAGCAGAAACTATTTTACAAAAAATTTCTCGCGGTAGTACTTTATTAGGCTATAGTGGGATGC
AGCCCAAGCTTTTTTTTGAAGAATTTATTTTTTTAAAACCTTTTTTGTATTTAGCTAAAGCAGAAATTATTGCTTATGCT
CAAAAAAATAAAATTGCTTATTTATCAGATTATACTAATCAATTAGATATTTATCAAAGAAATCAAATTAGAAATCATGT
GATTCCTTATTTGAAAGAAAAAACTAATTTTTTGCAAAATATAAAGAAGTTTTCTTGTCTTTTATCAGAAACCCAAGACT
TTATTAGAAAACAAACCCTTTTTTTTATTCAACACAACAAAGCCCCCAAAGAAAGTTTTTTTTCTTTTCAAAAAGATACC
AACTTTTTTTTAATTAAATCTTTTTTATTTCTGGATAAGGTGATTCAAAAAGATATTATTATTTATTTATTAGAGCAAAA
AAGGATTGATAAAAATTGGTTAGTAGTTGAAAGTATTATCAAAGGATTGGCTTTTGCTAAAAACCCTAATTTATCTTGGA
AGTTGAATTCCAATTACGATTTGATTAAAAGTTATAATTATTTTGGAATTATTGCTTTGGATGGGTTTTTAAAATTCAAA
AAAAAATTCTTCAAAAAACCTTTGCTTTACGCCTCAACTTGCAAAGTTTGTCTAGCTTCATTGCAACAAGTAATAACTCT
TAATTGCGATTTGGTAAACATTGCGTTTCCTCTGAAATTACGCCAAAGAAAACCAGGCGATTATTTAAAATTTAGTTTTG
GAAAACAAAAACTCAAAAACTTTTTAATCAATAAAAAAGTCCCTTTGTTCGAAAGAGATAATTTATGGCTCATAGTTGAT
AGTAAGGATAACGTTTTATGGATTCCTCATTTATATATTAATCATACCTTAGGAAACAAAGATTTGTTTTCTTTAGGTTT
AAAAAAAGAAACTTTTAATTATTTATAA

Upstream 100 bases:

>100_bases
AAAACGCAAAGTGCTAAAGATTTGATGCTAGATTCTCCAGGAGAAGTTGATCAAGAACAATTAAAAATTTTAAAACTTAA
ATGAGAAAAAACATGGTTCA

Downstream 100 bases:

>100_bases
AAGCAAAGGAAGGTGATTTTATGACGTTTTTAGATAAAATTTTTAAAAAATTTAATGTTCGTGTTTTGTATGTGGCTTTA
TTCATTATTATTGTTGCTTT

Product: Cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKLALKLDFQKTYIIAVSGGVDSMVLLDFLWSQKYKLKVVHFNHLKRLNAIKDKNLIQSYCDQRKIPFYYFELQLNPQNF
QTEARLLRQEKLKQIAKKYQTPYVITAHHLDDLAETILQKISRGSTLLGYSGMQPKLFFEEFIFLKPFLYLAKAEIIAYA
QKNKIAYLSDYTNQLDIYQRNQIRNHVIPYLKEKTNFLQNIKKFSCLLSETQDFIRKQTLFFIQHNKAPKESFFSFQKDT
NFFLIKSFLFLDKVIQKDIIIYLLEQKRIDKNWLVVESIIKGLAFAKNPNLSWKLNSNYDLIKSYNYFGIIALDGFLKFK
KKFFKKPLLYASTCKVCLASLQQVITLNCDLVNIAFPLKLRQRKPGDYLKFSFGKQKLKNFLINKKVPLFERDNLWLIVD
SKDNVLWIPHLYINHTLGNKDLFSLGLKKETFNYL

Sequences:

>Translated_435_residues
MKLALKLDFQKTYIIAVSGGVDSMVLLDFLWSQKYKLKVVHFNHLKRLNAIKDKNLIQSYCDQRKIPFYYFELQLNPQNF
QTEARLLRQEKLKQIAKKYQTPYVITAHHLDDLAETILQKISRGSTLLGYSGMQPKLFFEEFIFLKPFLYLAKAEIIAYA
QKNKIAYLSDYTNQLDIYQRNQIRNHVIPYLKEKTNFLQNIKKFSCLLSETQDFIRKQTLFFIQHNKAPKESFFSFQKDT
NFFLIKSFLFLDKVIQKDIIIYLLEQKRIDKNWLVVESIIKGLAFAKNPNLSWKLNSNYDLIKSYNYFGIIALDGFLKFK
KKFFKKPLLYASTCKVCLASLQQVITLNCDLVNIAFPLKLRQRKPGDYLKFSFGKQKLKNFLINKKVPLFERDNLWLIVD
SKDNVLWIPHLYINHTLGNKDLFSLGLKKETFNYL
>Mature_435_residues
MKLALKLDFQKTYIIAVSGGVDSMVLLDFLWSQKYKLKVVHFNHLKRLNAIKDKNLIQSYCDQRKIPFYYFELQLNPQNF
QTEARLLRQEKLKQIAKKYQTPYVITAHHLDDLAETILQKISRGSTLLGYSGMQPKLFFEEFIFLKPFLYLAKAEIIAYA
QKNKIAYLSDYTNQLDIYQRNQIRNHVIPYLKEKTNFLQNIKKFSCLLSETQDFIRKQTLFFIQHNKAPKESFFSFQKDT
NFFLIKSFLFLDKVIQKDIIIYLLEQKRIDKNWLVVESIIKGLAFAKNPNLSWKLNSNYDLIKSYNYFGIIALDGFLKFK
KKFFKKPLLYASTCKVCLASLQQVITLNCDLVNIAFPLKLRQRKPGDYLKFSFGKQKLKNFLINKKVPLFERDNLWLIVD
SKDNVLWIPHLYINHTLGNKDLFSLGLKKETFNYL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=230, Percent_Identity=27.3913043478261, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51522; Mature: 51522

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALKLDFQKTYIIAVSGGVDSMVLLDFLWSQKYKLKVVHFNHLKRLNAIKDKNLIQSY
CEEEEEEECCEEEEEEEECCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
CDQRKIPFYYFELQLNPQNFQTEARLLRQEKLKQIAKKYQTPYVITAHHLDDLAETILQK
HCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
ISRGSTLLGYSGMQPKLFFEEFIFLKPFLYLAKAEIIAYAQKNKIAYLSDYTNQLDIYQR
HHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCHHHHHH
NQIRNHVIPYLKEKTNFLQNIKKFSCLLSETQDFIRKQTLFFIQHNKAPKESFFSFQKDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCC
NFFLIKSFLFLDKVIQKDIIIYLLEQKRIDKNWLVVESIIKGLAFAKNPNLSWKLNSNYD
CHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
LIKSYNYFGIIALDGFLKFKKKFFKKPLLYASTCKVCLASLQQVITLNCDLVNIAFPLKL
EEECCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCEEEEEEECEEE
RQRKPGDYLKFSFGKQKLKNFLINKKVPLFERDNLWLIVDSKDNVLWIPHLYINHTLGNK
ECCCCCCCEEECCCHHHHHHHHHCCCCCCEECCCEEEEEECCCCEEEEEEEEEEECCCCC
DLFSLGLKKETFNYL
HHHHCCCCHHHCCCC
>Mature Secondary Structure
MKLALKLDFQKTYIIAVSGGVDSMVLLDFLWSQKYKLKVVHFNHLKRLNAIKDKNLIQSY
CEEEEEEECCEEEEEEEECCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
CDQRKIPFYYFELQLNPQNFQTEARLLRQEKLKQIAKKYQTPYVITAHHLDDLAETILQK
HCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
ISRGSTLLGYSGMQPKLFFEEFIFLKPFLYLAKAEIIAYAQKNKIAYLSDYTNQLDIYQR
HHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCHHHHHH
NQIRNHVIPYLKEKTNFLQNIKKFSCLLSETQDFIRKQTLFFIQHNKAPKESFFSFQKDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCC
NFFLIKSFLFLDKVIQKDIIIYLLEQKRIDKNWLVVESIIKGLAFAKNPNLSWKLNSNYD
CHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
LIKSYNYFGIIALDGFLKFKKKFFKKPLLYASTCKVCLASLQQVITLNCDLVNIAFPLKL
EEECCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCEEEEEEECEEE
RQRKPGDYLKFSFGKQKLKNFLINKKVPLFERDNLWLIVDSKDNVLWIPHLYINHTLGNK
ECCCCCCCEEECCCHHHHHHHHHCCCCCCEECCCEEEEEECCCCEEEEEEEEEEECCCCC
DLFSLGLKKETFNYL
HHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA