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The map label for this gene is dnaB [H]

Identifier: 197294440

GI number: 197294440

Start: 374384

End: 375883

Strand: Reverse

Name: dnaB [H]

Synonym: PAa_0361

Alternate gene names: 197294440

Gene position: 375883-374384 (Counterclockwise)

Preceding gene: 197294441

Following gene: 197294439

Centisome position: 42.72

GC content: 33.13

Gene sequence:

>1500_bases
ATGTTAACCAACGAACAAAAAGCCCTAAACCAAATTGTCAATTACATCCATCAAGAAAAATGGAAACATTTAGAATTTTA
TTGCGAAATCATCAACCCCAAACACTTACAAGACTCATTAAACCAAAAAATCTTTAACGCTTTAAAATATTTAAATCTAG
AAGGTCCATTCTCTCACCCTCTTAACAGAAGAAAAACCAGACAATTTATTATATCCGAATTATTGACTTATTTACAAAAA
CATTACCCTCAAGAAAATATCACCGCAAATCGCCTTAGTTTTTTAGATACCGACACTTCCTCAACCGACAACACACACCT
TCTAATTGATTTAAAACACACATACACCCAATCTCAACTCTATCAACAATTTTTAACAACCATCCAACCTTCTTTTGAAT
CCAAAGCTCCTCATCTTGTGGATTCACACATAAAAGAAACATTTGCCAAACTAAAAGATTTCATCTTCCTCATTCCTAAT
GAATCTGATAAAGAATTGCTTACCATAAGTCAAATGATGTCTTTAGGACTTGAATATTTATCACCTGATGTTTCTGCTAA
ACAAACAATCCAAGAAGAATATTATCGTTTATCCGATGATTTTGAAATGTTAAACAAAAACACTAAAGGATTTAAAAAAG
GTCAAGTTATCACAATCGGAGGGTACACAGGTTTAGGAAAAACTACTTTCGTCTATAATTTATTACTAGATTTAGCCCAA
ACCAGATTTAAAGAATCAGAAGAACAACCACACATGATTGTTTTTTCATACGAAATGACTTGGCAAGAAAACTTAACTCG
CTTATTAGCACGCCATACCCAAATTCCTTTAAATGTTATTTTGGATAAAGACTTTGACCCTCAAGCAACCGACGTTACAC
AATCAATTTATGACGAAAGGATGGAATCAGCAAAAAGTTTTATCTCAAACCTTAATTTATCTTTTAGTTATACCAAAACC
AAAAGCATCAATTACATCGTCGATTTACTTTATCGCCTTCATTTAGAGAAAAAAGTAGATGTCGTTGTAATCGACCATCT
CCAAATAACCAAATCAACCAAACACTTAGAAAACGACCGTTTGGCAATTGATGAAATCATGGAAAAAATCAAAGAACTCG
CAACCAAATTAGAGATTGTTGTTATTATTTTATCACAATTCTCTAGAGACACTTACAGCAATTACCAAGGCAAATCCCCC
GAAATAACTGCTTTAAAAGGCTCTGGAGGAATCGAGACAAACTCTGACATTGTTTTAATAATGTCTGAATTCAACCCAAA
AATATCCAAAGACAAAGATAAACTTATTTCTTCCAACAACGAAACCGTAAGTCAATTATACGAAGACGCCACAGGAAAAG
ACAACCAAAAAATTATTGAAGTCAACATCAAGAAAAACCGCAGCGGCACCAAACAAACTTTAGTTTATCACTTCGCTATG
GAAACACAAACATTTAAGGAAGTAGGTTTTGTTCTTCCTCACGAAATAGATGCCAATTAA

Upstream 100 bases:

>100_bases
TCACCAAACAGCCACTCATTTTTTCAAAACTTTTCCCGAAGACAATTTATTACCTTTCCAAATATAAATTTAATTAAAAA
ATAAAAGGAGATTTAAAAAA

Downstream 100 bases:

>100_bases
GGAGTAAAAAAATGAATATGCAAGACAAAATCAAATACATTCAAACCAACTTCCCGATGATGGTTTTATTAATCAAATTA
AAAGTTCTACCACCCAATTT

Product: Replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLTNEQKALNQIVNYIHQEKWKHLEFYCEIINPKHLQDSLNQKIFNALKYLNLEGPFSHPLNRRKTRQFIISELLTYLQK
HYPQENITANRLSFLDTDTSSTDNTHLLIDLKHTYTQSQLYQQFLTTIQPSFESKAPHLVDSHIKETFAKLKDFIFLIPN
ESDKELLTISQMMSLGLEYLSPDVSAKQTIQEEYYRLSDDFEMLNKNTKGFKKGQVITIGGYTGLGKTTFVYNLLLDLAQ
TRFKESEEQPHMIVFSYEMTWQENLTRLLARHTQIPLNVILDKDFDPQATDVTQSIYDERMESAKSFISNLNLSFSYTKT
KSINYIVDLLYRLHLEKKVDVVVIDHLQITKSTKHLENDRLAIDEIMEKIKELATKLEIVVIILSQFSRDTYSNYQGKSP
EITALKGSGGIETNSDIVLIMSEFNPKISKDKDKLISSNNETVSQLYEDATGKDNQKIIEVNIKKNRSGTKQTLVYHFAM
ETQTFKEVGFVLPHEIDAN

Sequences:

>Translated_499_residues
MLTNEQKALNQIVNYIHQEKWKHLEFYCEIINPKHLQDSLNQKIFNALKYLNLEGPFSHPLNRRKTRQFIISELLTYLQK
HYPQENITANRLSFLDTDTSSTDNTHLLIDLKHTYTQSQLYQQFLTTIQPSFESKAPHLVDSHIKETFAKLKDFIFLIPN
ESDKELLTISQMMSLGLEYLSPDVSAKQTIQEEYYRLSDDFEMLNKNTKGFKKGQVITIGGYTGLGKTTFVYNLLLDLAQ
TRFKESEEQPHMIVFSYEMTWQENLTRLLARHTQIPLNVILDKDFDPQATDVTQSIYDERMESAKSFISNLNLSFSYTKT
KSINYIVDLLYRLHLEKKVDVVVIDHLQITKSTKHLENDRLAIDEIMEKIKELATKLEIVVIILSQFSRDTYSNYQGKSP
EITALKGSGGIETNSDIVLIMSEFNPKISKDKDKLISSNNETVSQLYEDATGKDNQKIIEVNIKKNRSGTKQTLVYHFAM
ETQTFKEVGFVLPHEIDAN
>Mature_499_residues
MLTNEQKALNQIVNYIHQEKWKHLEFYCEIINPKHLQDSLNQKIFNALKYLNLEGPFSHPLNRRKTRQFIISELLTYLQK
HYPQENITANRLSFLDTDTSSTDNTHLLIDLKHTYTQSQLYQQFLTTIQPSFESKAPHLVDSHIKETFAKLKDFIFLIPN
ESDKELLTISQMMSLGLEYLSPDVSAKQTIQEEYYRLSDDFEMLNKNTKGFKKGQVITIGGYTGLGKTTFVYNLLLDLAQ
TRFKESEEQPHMIVFSYEMTWQENLTRLLARHTQIPLNVILDKDFDPQATDVTQSIYDERMESAKSFISNLNLSFSYTKT
KSINYIVDLLYRLHLEKKVDVVVIDHLQITKSTKHLENDRLAIDEIMEKIKELATKLEIVVIILSQFSRDTYSNYQGKSP
EITALKGSGGIETNSDIVLIMSEFNPKISKDKDKLISSNNETVSQLYEDATGKDNQKIIEVNIKKNRSGTKQTLVYHFAM
ETQTFKEVGFVLPHEIDAN

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=221, Percent_Identity=25.7918552036199, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 57855; Mature: 57855

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNEQKALNQIVNYIHQEKWKHLEFYCEIINPKHLQDSLNQKIFNALKYLNLEGPFSHP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LNRRKTRQFIISELLTYLQKHYPQENITANRLSFLDTDTSSTDNTHLLIDLKHTYTQSQL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEECCCCCCCCEEEEEEEHHHHHHHHH
YQQFLTTIQPSFESKAPHLVDSHIKETFAKLKDFIFLIPNESDKELLTISQMMSLGLEYL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHH
SPDVSAKQTIQEEYYRLSDDFEMLNKNTKGFKKGQVITIGGYTGLGKTTFVYNLLLDLAQ
CCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
TRFKESEEQPHMIVFSYEMTWQENLTRLLARHTQIPLNVILDKDFDPQATDVTQSIYDER
HHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
MESAKSFISNLNLSFSYTKTKSINYIVDLLYRLHLEKKVDVVVIDHLQITKSTKHLENDR
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHCCCC
LAIDEIMEKIKELATKLEIVVIILSQFSRDTYSNYQGKSPEITALKGSGGIETNSDIVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEEE
MSEFNPKISKDKDKLISSNNETVSQLYEDATGKDNQKIIEVNIKKNRSGTKQTLVYHFAM
EECCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHEEEEEEHH
ETQTFKEVGFVLPHEIDAN
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTNEQKALNQIVNYIHQEKWKHLEFYCEIINPKHLQDSLNQKIFNALKYLNLEGPFSHP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LNRRKTRQFIISELLTYLQKHYPQENITANRLSFLDTDTSSTDNTHLLIDLKHTYTQSQL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEECCCCCCCCEEEEEEEHHHHHHHHH
YQQFLTTIQPSFESKAPHLVDSHIKETFAKLKDFIFLIPNESDKELLTISQMMSLGLEYL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHH
SPDVSAKQTIQEEYYRLSDDFEMLNKNTKGFKKGQVITIGGYTGLGKTTFVYNLLLDLAQ
CCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
TRFKESEEQPHMIVFSYEMTWQENLTRLLARHTQIPLNVILDKDFDPQATDVTQSIYDER
HHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
MESAKSFISNLNLSFSYTKTKSINYIVDLLYRLHLEKKVDVVVIDHLQITKSTKHLENDR
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHCCCC
LAIDEIMEKIKELATKLEIVVIILSQFSRDTYSNYQGKSPEITALKGSGGIETNSDIVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEEE
MSEFNPKISKDKDKLISSNNETVSQLYEDATGKDNQKIIEVNIKKNRSGTKQTLVYHFAM
EECCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHEEEEEEHH
ETQTFKEVGFVLPHEIDAN
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]