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The map label for this gene is dnaG [H]

Identifier: 197294439

GI number: 197294439

Start: 372576

End: 374372

Strand: Reverse

Name: dnaG [H]

Synonym: PAa_0360

Alternate gene names: 197294439

Gene position: 374372-372576 (Counterclockwise)

Preceding gene: 197294440

Following gene: 197294438

Centisome position: 42.54

GC content: 34.95

Gene sequence:

>1797_bases
ATGAATATGCAAGACAAAATCAAATACATTCAAACCAACTTCCCGATGATGGTTTTATTAATCAAATTAAAAGTTCTACC
ACCCAATTTCAATCGTAAATATCGCTTCCCTTGTCCCATTCATCAAGGCAAAAACCCTACTTGCTGTCGTATCACCGAGG
ATAACGCGATACATTGTTTCAAATGTACTAAACGATACGATATTGTTGATGTCTATCAAAGAACTAGAGGACTAGATTTT
AATGAATCTATCCGAAGAATCCTCAGTTTTATGAAATCGCAAGAATTTAAAACTTTACTCCAAGAAGAGCAACAAATAAC
TAAACTAACAAAAAGTCCTTTCAAACAACAATATCATTCAAAATCAGCTTACCCAACGGACGAAACACAATTAAAAACCG
CCAAAAGGAAATTACTCAAAGAAGTATCGCCCTTATTCAACCAAATCCGCGATTACTACCACTATTTATTAACTGCCAAC
CAAAACAACGAATCTCACCAAGGGATAAAATACTTGACCGAAAAAAGAAAGCTCACCCCAGCCACAATTAAAGAATTCAG
CCTGGGGTTCGCTCCTCTATCCGATAAACCTTTATCTTTCCGATTCTTTAATTATTGCCAAAAGAAAAACATTAACATCC
ACAAGTTAATAGAATACGGCTTTATCAAAGAAAAAACCAGCAAAAAAGGCTCAAAATATTATCACGATGCTTTTAATGGC
TCTGTTATTATCCCAATAGAAAATGGACGCCAACACACTTTTCATTTTTACCAAAACAATTTCCAAAACACTGGCATTTT
TAAACCCAAATATCAATCCCTAACTAACTTCTCTCAAACACCTACTTTTTATTTCAGCTATCGCTTCTTTGAGGCATTAC
CTTACATCAAACAAGCAAAAACCGTCATTATCCATGAAGGTTTTTTTGATGTAATCAGTTGTTGGCAACGCGGAATCAAA
AATGTAGTGGGATTAATTTGCATCGCTCAACAACTATCCCAAGCTCAAATTGATATTTTCAAAAAAGAACAAATAAAGGT
AATCATCGCTTTAGACAACGACGAAACAGGGCTCAACCGCAACAAAGAATTAGCGGCTCAACTCAAATCCAACCAGGTTG
ATTGTGAAATACGCAGAATTTTACCACCATACTCCAAAACTTGCAAAGATGTAGATGATTTATTACGCAATCATGGGTAT
CAAGCATATAAAAAATGCTTTCTTGACAATTCTGACCCTTATGCAGGCTACGAAGGCTACCCCCCATATGATTTCGCCGT
TAACTTATTTGGCAAAAACACAGTAGAAATAGTTAATTCCAAACGGTCCTTAAACTCAAAAGAATCACCTACACTCCAAA
CCAAAAAACCAACAACAAAACAACAAATAACAACTTACCCAGATGGCAGCAAAACAATTCACGAAATAGACCTCAAAACA
AACCAAATAATAAAAACTACTGACACCCATCCCAGCGGCTCAAAATATATTGTAGAAGATAAAGGGTTGATAATTACAAC
AACCTACTATGAACCTGATGACGAGTTTTGTTGGAGCGAAAAACGCGAATTCGACTCTGAAACTCGCGAAGTAATCAAAA
GGACTTATAAAAGTTACAAAGGCACAAAAGAAATTAAAGAATTCGACCCCAAAACAAACAACATCACCAAATCAACCAAA
TACAAAAACAAAAAACTTTTTTCTATCAAAGAATACGACCCTCAACAAACAGATAAATTAATCAAATACACTGAATATAA
CGAGGATAATACTTATCATGTCAAAAATTACGACTAA

Upstream 100 bases:

>100_bases
AACAAACTTTAGTTTATCACTTCGCTATGGAAACACAAACATTTAAGGAAGTAGGTTTTGTTCTTCCTCACGAAATAGAT
GCCAATTAAGGAGTAAAAAA

Downstream 100 bases:

>100_bases
ACAACTGGCAAACAAACCGCAGAGACCATCTACAATCCCAACAACACCTTCAAAGAAGCTAAAAACTTCAATCAACCACA
ATCCCTTTAAGGGCTTTTTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MNMQDKIKYIQTNFPMMVLLIKLKVLPPNFNRKYRFPCPIHQGKNPTCCRITEDNAIHCFKCTKRYDIVDVYQRTRGLDF
NESIRRILSFMKSQEFKTLLQEEQQITKLTKSPFKQQYHSKSAYPTDETQLKTAKRKLLKEVSPLFNQIRDYYHYLLTAN
QNNESHQGIKYLTEKRKLTPATIKEFSLGFAPLSDKPLSFRFFNYCQKKNINIHKLIEYGFIKEKTSKKGSKYYHDAFNG
SVIIPIENGRQHTFHFYQNNFQNTGIFKPKYQSLTNFSQTPTFYFSYRFFEALPYIKQAKTVIIHEGFFDVISCWQRGIK
NVVGLICIAQQLSQAQIDIFKKEQIKVIIALDNDETGLNRNKELAAQLKSNQVDCEIRRILPPYSKTCKDVDDLLRNHGY
QAYKKCFLDNSDPYAGYEGYPPYDFAVNLFGKNTVEIVNSKRSLNSKESPTLQTKKPTTKQQITTYPDGSKTIHEIDLKT
NQIIKTTDTHPSGSKYIVEDKGLIITTTYYEPDDEFCWSEKREFDSETREVIKRTYKSYKGTKEIKEFDPKTNNITKSTK
YKNKKLFSIKEYDPQQTDKLIKYTEYNEDNTYHVKNYD

Sequences:

>Translated_598_residues
MNMQDKIKYIQTNFPMMVLLIKLKVLPPNFNRKYRFPCPIHQGKNPTCCRITEDNAIHCFKCTKRYDIVDVYQRTRGLDF
NESIRRILSFMKSQEFKTLLQEEQQITKLTKSPFKQQYHSKSAYPTDETQLKTAKRKLLKEVSPLFNQIRDYYHYLLTAN
QNNESHQGIKYLTEKRKLTPATIKEFSLGFAPLSDKPLSFRFFNYCQKKNINIHKLIEYGFIKEKTSKKGSKYYHDAFNG
SVIIPIENGRQHTFHFYQNNFQNTGIFKPKYQSLTNFSQTPTFYFSYRFFEALPYIKQAKTVIIHEGFFDVISCWQRGIK
NVVGLICIAQQLSQAQIDIFKKEQIKVIIALDNDETGLNRNKELAAQLKSNQVDCEIRRILPPYSKTCKDVDDLLRNHGY
QAYKKCFLDNSDPYAGYEGYPPYDFAVNLFGKNTVEIVNSKRSLNSKESPTLQTKKPTTKQQITTYPDGSKTIHEIDLKT
NQIIKTTDTHPSGSKYIVEDKGLIITTTYYEPDDEFCWSEKREFDSETREVIKRTYKSYKGTKEIKEFDPKTNNITKSTK
YKNKKLFSIKEYDPQQTDKLIKYTEYNEDNTYHVKNYD
>Mature_598_residues
MNMQDKIKYIQTNFPMMVLLIKLKVLPPNFNRKYRFPCPIHQGKNPTCCRITEDNAIHCFKCTKRYDIVDVYQRTRGLDF
NESIRRILSFMKSQEFKTLLQEEQQITKLTKSPFKQQYHSKSAYPTDETQLKTAKRKLLKEVSPLFNQIRDYYHYLLTAN
QNNESHQGIKYLTEKRKLTPATIKEFSLGFAPLSDKPLSFRFFNYCQKKNINIHKLIEYGFIKEKTSKKGSKYYHDAFNG
SVIIPIENGRQHTFHFYQNNFQNTGIFKPKYQSLTNFSQTPTFYFSYRFFEALPYIKQAKTVIIHEGFFDVISCWQRGIK
NVVGLICIAQQLSQAQIDIFKKEQIKVIIALDNDETGLNRNKELAAQLKSNQVDCEIRRILPPYSKTCKDVDDLLRNHGY
QAYKKCFLDNSDPYAGYEGYPPYDFAVNLFGKNTVEIVNSKRSLNSKESPTLQTKKPTTKQQITTYPDGSKTIHEIDLKT
NQIIKTTDTHPSGSKYIVEDKGLIITTTYYEPDDEFCWSEKREFDSETREVIKRTYKSYKGTKEIKEFDPKTNNITKSTK
YKNKKLFSIKEYDPQQTDKLIKYTEYNEDNTYHVKNYD

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70422; Mature: 70422

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMQDKIKYIQTNFPMMVLLIKLKVLPPNFNRKYRFPCPIHQGKNPTCCRITEDNAIHCF
CCCHHHHHHHHCCCCHHEEEHHHHCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCEEEE
KCTKRYDIVDVYQRTRGLDFNESIRRILSFMKSQEFKTLLQEEQQITKLTKSPFKQQYHS
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KSAYPTDETQLKTAKRKLLKEVSPLFNQIRDYYHYLLTANQNNESHQGIKYLTEKRKLTP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCH
ATIKEFSLGFAPLSDKPLSFRFFNYCQKKNINIHKLIEYGFIKEKTSKKGSKYYHDAFNG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCC
SVIIPIENGRQHTFHFYQNNFQNTGIFKPKYQSLTNFSQTPTFYFSYRFFEALPYIKQAK
EEEEEECCCCCEEEEHHHCCCCCCCCCCCCHHHHCCCCCCCEEEEEHHHHHHCCHHHHCC
TVIIHEGFFDVISCWQRGIKNVVGLICIAQQLSQAQIDIFKKEQIKVIIALDNDETGLNR
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEEEECCCCCCCCC
NKELAAQLKSNQVDCEIRRILPPYSKTCKDVDDLLRNHGYQAYKKCFLDNSDPYAGYEGY
CHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
PPYDFAVNLFGKNTVEIVNSKRSLNSKESPTLQTKKPTTKQQITTYPDGSKTIHEIDLKT
CCHHHEEEECCCCEEEEHHCHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCEEEEEECCC
NQIIKTTDTHPSGSKYIVEDKGLIITTTYYEPDDEFCWSEKREFDSETREVIKRTYKSYK
CCEEEECCCCCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
GTKEIKEFDPKTNNITKSTKYKNKKLFSIKEYDPQQTDKLIKYTEYNEDNTYHVKNYD
CHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHEEEEECCCCCCEEECCCC
>Mature Secondary Structure
MNMQDKIKYIQTNFPMMVLLIKLKVLPPNFNRKYRFPCPIHQGKNPTCCRITEDNAIHCF
CCCHHHHHHHHCCCCHHEEEHHHHCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCEEEE
KCTKRYDIVDVYQRTRGLDFNESIRRILSFMKSQEFKTLLQEEQQITKLTKSPFKQQYHS
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KSAYPTDETQLKTAKRKLLKEVSPLFNQIRDYYHYLLTANQNNESHQGIKYLTEKRKLTP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCH
ATIKEFSLGFAPLSDKPLSFRFFNYCQKKNINIHKLIEYGFIKEKTSKKGSKYYHDAFNG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCC
SVIIPIENGRQHTFHFYQNNFQNTGIFKPKYQSLTNFSQTPTFYFSYRFFEALPYIKQAK
EEEEEECCCCCEEEEHHHCCCCCCCCCCCCHHHHCCCCCCCEEEEEHHHHHHCCHHHHCC
TVIIHEGFFDVISCWQRGIKNVVGLICIAQQLSQAQIDIFKKEQIKVIIALDNDETGLNR
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEEEECCCCCCCCC
NKELAAQLKSNQVDCEIRRILPPYSKTCKDVDDLLRNHGYQAYKKCFLDNSDPYAGYEGY
CHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
PPYDFAVNLFGKNTVEIVNSKRSLNSKESPTLQTKKPTTKQQITTYPDGSKTIHEIDLKT
CCHHHEEEECCCCEEEEHHCHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCEEEEEECCC
NQIIKTTDTHPSGSKYIVEDKGLIITTTYYEPDDEFCWSEKREFDSETREVIKRTYKSYK
CCEEEECCCCCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
GTKEIKEFDPKTNNITKSTKYKNKKLFSIKEYDPQQTDKLIKYTEYNEDNTYHVKNYD
CHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHEEEEECCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]