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The map label for this gene is ssb [H]

Identifier: 197294288

GI number: 197294288

Start: 180927

End: 181361

Strand: Reverse

Name: ssb [H]

Synonym: PAa_0151

Alternate gene names: 197294288

Gene position: 181361-180927 (Counterclockwise)

Preceding gene: 197294289

Following gene: 197294287

Centisome position: 20.61

GC content: 26.9

Gene sequence:

>435_bases
ATGATTAATAAAGTTATTTTAGTTGGAAGAATTACTAAAGACCCTGAATTAAAATCTTATAATGGGGAAGTTAATTATGT
TAAATTTACCTTAGCAGTAAATAGATTTTATAACAATCAAGAAGAAAATAAAAAAACTGATTTCATTAATTGCATCGTTT
GGCGTAAACAAGCGGAAAATTTGAGTAAATACATCTTTAAAGGTAATTTATTAGGAGTTGAAGGAAGCATTCGAGTAGAA
AAATGGGAAAAAGATAATAAAACCAATTGGTCTACTGAAATTCTTTGCAATAACATTTCTTTTTTAGAATCCAAGAAAAG
TTTGAACGCAACACAAGAAAAAGACTATCAATCAAATAATGACTCAAGTTTTGAAAATTCACAATCTAATGATTTTGAAA
CAAATACACCAGAAGATGATGGATTACCCTTTTAA

Upstream 100 bases:

>100_bases
AGTCCTTAAATGATTATTTTGTGAATTTTAAAATTATTAACAAAAGCAATAAGTGAAGCAATAAAGAAATGAAATAGTTA
TAAAAGGAGATTTAATCTAG

Downstream 100 bases:

>100_bases
ATTAAGAAAGAGGCAAAATAAAAATGAAAAAAAATAAAAGACATGTTTTCAAAAAACGTCGCAAAGTTTGTTATTTTACT
GAAAACAAATTCAGTAAAAT

Product: Single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB; Helix-destabilizing protein [H]

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MINKVILVGRITKDPELKSYNGEVNYVKFTLAVNRFYNNQEENKKTDFINCIVWRKQAENLSKYIFKGNLLGVEGSIRVE
KWEKDNKTNWSTEILCNNISFLESKKSLNATQEKDYQSNNDSSFENSQSNDFETNTPEDDGLPF

Sequences:

>Translated_144_residues
MINKVILVGRITKDPELKSYNGEVNYVKFTLAVNRFYNNQEENKKTDFINCIVWRKQAENLSKYIFKGNLLGVEGSIRVE
KWEKDNKTNWSTEILCNNISFLESKKSLNATQEKDYQSNNDSSFENSQSNDFETNTPEDDGLPF
>Mature_144_residues
MINKVILVGRITKDPELKSYNGEVNYVKFTLAVNRFYNNQEENKKTDFINCIVWRKQAENLSKYIFKGNLLGVEGSIRVE
KWEKDNKTNWSTEILCNNISFLESKKSLNATQEKDYQSNNDSSFENSQSNDFETNTPEDDGLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

None

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 16750; Mature: 16750

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKVILVGRITKDPELKSYNGEVNYVKFTLAVNRFYNNQEENKKTDFINCIVWRKQAEN
CCCEEEEEEECCCCCCCCCCCCCEEEEEEEEEEEHHHCCCCCCCCCCCEEHEEEHHHHHH
LSKYIFKGNLLGVEGSIRVEKWEKDNKTNWSTEILCNNISFLESKKSLNATQEKDYQSNN
HHHHHHCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCC
DSSFENSQSNDFETNTPEDDGLPF
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINKVILVGRITKDPELKSYNGEVNYVKFTLAVNRFYNNQEENKKTDFINCIVWRKQAEN
CCCEEEEEEECCCCCCCCCCCCCEEEEEEEEEEEHHHCCCCCCCCCCCEEHEEEHHHHHH
LSKYIFKGNLLGVEGSIRVEKWEKDNKTNWSTEILCNNISFLESKKSLNATQEKDYQSNN
HHHHHHCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCC
DSSFENSQSNDFETNTPEDDGLPF
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]