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The map label for this gene is metG [H]

Identifier: 197294265

GI number: 197294265

Start: 145853

End: 147406

Strand: Reverse

Name: metG [H]

Synonym: PAa_0128

Alternate gene names: 197294265

Gene position: 147406-145853 (Counterclockwise)

Preceding gene: 197294266

Following gene: 197294264

Centisome position: 16.75

GC content: 28.89

Gene sequence:

>1554_bases
ATGGAAAACAAAACTAAATTTTACCTTTCAACTTCCATTGCTTACGCTTCTGGAATACCGCATATTGGAAATGTTTACGA
AGTTATTTTAGCTGATGCAATTGCTCGATTTAAGCGTTTAGATGGTTGGGATGTTTTTTTTCAAACAGGAACAGACGAAC
ATGGACAAAAAATTGAACAAAAAGCTTCTTCAAAAGGGGTTCTTCCACAAGAATACGTTAATTATATTTCTTCGGAAATT
AAAAGAATTTATGATTTAATGCAAATTAGTTATGATCGCTTTATTAAAACAACCAATCATAAACATCAAAAAGCAGTTCA
AGTGATTTTTACAAAATTATTGCAACAAGGAGATATTTATTTAGGCAAATACCAAGGATTGTATTCAGTGACTGAAGAGG
TTTATGTATCAGAAAAAGATTTAGTGGATGGAAAAACTTTCAATGGTGAAATCCCCATTTTAATTAGTGAAGATACTTAC
TTTTTTAAATTAGCAAAATATCAAAACCGTCTTTTGGATTATTTAGAAAATAACCCTAATTTAATCCAACCAGAAACACA
AAAAAAAGAAATTTTAAATCTTTTAAAAGAACCGTTAAATGATTTATCAATTTCAAGAACATCTTTTCGATGGGGAATCC
CTGTTCCTTGTGATAAAAACCATGTTATTTATGTTTGGATTGATGCTTTAAGTAATTATTTAACTGGTTTAGATTATAAC
CCTAATGAAAACACTAGTTCCAAGTTTTTACAATATTGGCCTTGTAATTTGCATGTCATTGGGAAAGATATTTTAAGATT
TCATTTAATTTATTGGCCTATTTTACTAATGGCTCTTAAAATTGAGTTGCCCCAAAAATTTTTAGCTCATCCTTGGATTT
TATTTGATAAAAATAAAATGTCTAAATCAAAAGGCAACGTTTTATATGTAGATGATTTATTAAAATTTTTTCCCATTGAC
ACTATTCGTTATTTTGTTTTACATGAAATCCCTTATGCAAGTGACGGAAATATTACTTATGAATTGTTAGTTGAAAGACA
TAATAGTGTTTTAGTCAATCTTTTTGGCAACGTAGTTCATCGTATTTTTGGAATGTTAAAAAGTTATCGTAATTACCAAC
TTACTCAAATTTTGGATCTTCCTGAAAATCACCAAGAGTTTGATTTATCGCAAAAAGTTTTGTTAGTTTTGCCTTTAATG
CGTCAAAAATTTGAAGAGTGTAAAGTGGGTGAGGCCTTGGAAGAAGTAGAAAAATTAGCGCGATTAATGAATAAATACAT
TGATTCTGTTAAACCTTGGGAATTAGCACAATTACCCGAAAAAGAAACTTTATTAAATTATGTTTTATATTCTTTGACAG
AAGCTTTGAGGTTTTTAGGCGTTTTATTAAAACCTTTTTTACCACAAACAGCAGAAAAAATCCTTTGTCAAATTCAGGCA
GAAGATGTTACTTTTGAAAGTTTAAAAACTTTTGGTTTGTTGAAAACTAAAAAATTGATGCCAGATGAAATGTTATTTCA
GCGCATAGATTTAGAAAATGTTTTACCACAATAA

Upstream 100 bases:

>100_bases
ATGCCGATAAAAGAAATTGTTTTTAATGAAGATGGTACTATCAAAGAAATGAAAAAATTTTAATTTTTAGAACTAAAATA
ATTTTTAATAAAGGAGTTTT

Downstream 100 bases:

>100_bases
ATTATTATTCACGATAATAACAAACAAAAATTAAATTTTTTAAGAAAGCTTTGTTAAAGAAATTAGTTTTATAATGATTT
TTGCAAAATCTAGAGGAAAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MENKTKFYLSTSIAYASGIPHIGNVYEVILADAIARFKRLDGWDVFFQTGTDEHGQKIEQKASSKGVLPQEYVNYISSEI
KRIYDLMQISYDRFIKTTNHKHQKAVQVIFTKLLQQGDIYLGKYQGLYSVTEEVYVSEKDLVDGKTFNGEIPILISEDTY
FFKLAKYQNRLLDYLENNPNLIQPETQKKEILNLLKEPLNDLSISRTSFRWGIPVPCDKNHVIYVWIDALSNYLTGLDYN
PNENTSSKFLQYWPCNLHVIGKDILRFHLIYWPILLMALKIELPQKFLAHPWILFDKNKMSKSKGNVLYVDDLLKFFPID
TIRYFVLHEIPYASDGNITYELLVERHNSVLVNLFGNVVHRIFGMLKSYRNYQLTQILDLPENHQEFDLSQKVLLVLPLM
RQKFEECKVGEALEEVEKLARLMNKYIDSVKPWELAQLPEKETLLNYVLYSLTEALRFLGVLLKPFLPQTAEKILCQIQA
EDVTFESLKTFGLLKTKKLMPDEMLFQRIDLENVLPQ

Sequences:

>Translated_517_residues
MENKTKFYLSTSIAYASGIPHIGNVYEVILADAIARFKRLDGWDVFFQTGTDEHGQKIEQKASSKGVLPQEYVNYISSEI
KRIYDLMQISYDRFIKTTNHKHQKAVQVIFTKLLQQGDIYLGKYQGLYSVTEEVYVSEKDLVDGKTFNGEIPILISEDTY
FFKLAKYQNRLLDYLENNPNLIQPETQKKEILNLLKEPLNDLSISRTSFRWGIPVPCDKNHVIYVWIDALSNYLTGLDYN
PNENTSSKFLQYWPCNLHVIGKDILRFHLIYWPILLMALKIELPQKFLAHPWILFDKNKMSKSKGNVLYVDDLLKFFPID
TIRYFVLHEIPYASDGNITYELLVERHNSVLVNLFGNVVHRIFGMLKSYRNYQLTQILDLPENHQEFDLSQKVLLVLPLM
RQKFEECKVGEALEEVEKLARLMNKYIDSVKPWELAQLPEKETLLNYVLYSLTEALRFLGVLLKPFLPQTAEKILCQIQA
EDVTFESLKTFGLLKTKKLMPDEMLFQRIDLENVLPQ
>Mature_517_residues
MENKTKFYLSTSIAYASGIPHIGNVYEVILADAIARFKRLDGWDVFFQTGTDEHGQKIEQKASSKGVLPQEYVNYISSEI
KRIYDLMQISYDRFIKTTNHKHQKAVQVIFTKLLQQGDIYLGKYQGLYSVTEEVYVSEKDLVDGKTFNGEIPILISEDTY
FFKLAKYQNRLLDYLENNPNLIQPETQKKEILNLLKEPLNDLSISRTSFRWGIPVPCDKNHVIYVWIDALSNYLTGLDYN
PNENTSSKFLQYWPCNLHVIGKDILRFHLIYWPILLMALKIELPQKFLAHPWILFDKNKMSKSKGNVLYVDDLLKFFPID
TIRYFVLHEIPYASDGNITYELLVERHNSVLVNLFGNVVHRIFGMLKSYRNYQLTQILDLPENHQEFDLSQKVLLVLPLM
RQKFEECKVGEALEEVEKLARLMNKYIDSVKPWELAQLPEKETLLNYVLYSLTEALRFLGVLLKPFLPQTAEKILCQIQA
EDVTFESLKTFGLLKTKKLMPDEMLFQRIDLENVLPQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=543, Percent_Identity=32.5966850828729, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI14043022, Length=514, Percent_Identity=24.7081712062257, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1788432, Length=546, Percent_Identity=24.5421245421245, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1786861, Length=192, Percent_Identity=23.9583333333333, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=496, Percent_Identity=30.8467741935484, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=30.2872062663185, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17540794, Length=564, Percent_Identity=23.4042553191489, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=29.6903460837887, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=24.1007194244604, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21358483, Length=544, Percent_Identity=33.2720588235294, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24655311, Length=538, Percent_Identity=23.6059479553903, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 60412; Mature: 60412

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKTKFYLSTSIAYASGIPHIGNVYEVILADAIARFKRLDGWDVFFQTGTDEHGQKIEQ
CCCCEEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
KASSKGVLPQEYVNYISSEIKRIYDLMQISYDRFIKTTNHKHQKAVQVIFTKLLQQGDIY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEE
LGKYQGLYSVTEEVYVSEKDLVDGKTFNGEIPILISEDTYFFKLAKYQNRLLDYLENNPN
EECCCCHHHHHHHHHCCCHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCC
LIQPETQKKEILNLLKEPLNDLSISRTSFRWGIPVPCDKNHVIYVWIDALSNYLTGLDYN
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEEEEHHHHHHHHCCCCCC
PNENTSSKFLQYWPCNLHVIGKDILRFHLIYWPILLMALKIELPQKFLAHPWILFDKNKM
CCCCCHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCC
SKSKGNVLYVDDLLKFFPIDTIRYFVLHEIPYASDGNITYELLVERHNSVLVNLFGNVVH
CCCCCCEEEHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHH
RIFGMLKSYRNYQLTQILDLPENHQEFDLSQKVLLVLPLMRQKFEECKVGEALEEVEKLA
HHHHHHHHHCCCCHHHHCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMNKYIDSVKPWELAQLPEKETLLNYVLYSLTEALRFLGVLLKPFLPQTAEKILCQIQA
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EDVTFESLKTFGLLKTKKLMPDEMLFQRIDLENVLPQ
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MENKTKFYLSTSIAYASGIPHIGNVYEVILADAIARFKRLDGWDVFFQTGTDEHGQKIEQ
CCCCEEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
KASSKGVLPQEYVNYISSEIKRIYDLMQISYDRFIKTTNHKHQKAVQVIFTKLLQQGDIY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEE
LGKYQGLYSVTEEVYVSEKDLVDGKTFNGEIPILISEDTYFFKLAKYQNRLLDYLENNPN
EECCCCHHHHHHHHHCCCHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCC
LIQPETQKKEILNLLKEPLNDLSISRTSFRWGIPVPCDKNHVIYVWIDALSNYLTGLDYN
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEEEEHHHHHHHHCCCCCC
PNENTSSKFLQYWPCNLHVIGKDILRFHLIYWPILLMALKIELPQKFLAHPWILFDKNKM
CCCCCHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCC
SKSKGNVLYVDDLLKFFPIDTIRYFVLHEIPYASDGNITYELLVERHNSVLVNLFGNVVH
CCCCCCEEEHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHH
RIFGMLKSYRNYQLTQILDLPENHQEFDLSQKVLLVLPLMRQKFEECKVGEALEEVEKLA
HHHHHHHHHCCCCHHHHCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMNKYIDSVKPWELAQLPEKETLLNYVLYSLTEALRFLGVLLKPFLPQTAEKILCQIQA
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EDVTFESLKTFGLLKTKKLMPDEMLFQRIDLENVLPQ
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]