Click here to switch to the map view.

The map label for this gene is sau96IM [H]

Identifier: 197294172

GI number: 197294172

Start: 4983

End: 5816

Strand: Direct

Name: sau96IM [H]

Synonym: PAa_0004

Alternate gene names: 197294172

Gene position: 4983-5816 (Clockwise)

Preceding gene: 197294171

Following gene: 197294173

Centisome position: 0.57

GC content: 28.06

Gene sequence:

>834_bases
ATGATATTTGGCAAGAATACGCTCAACTTCAAGCCAAAAAAAGGTAGCAAAAGAAAAAAGAATTTATCTCATGAATTAGA
AAATAAACGTCTTTTGGAATTAAAAAAACAAAAAAATGATTTATTAAAAAAATTAGAAAAACAACAAGTTTTAATTTCTG
TATTTGATGATATTAAAAATAAATACGATAAAATGGGTTATAAAGTTTACCATCAAGTATTAAATTCAGCTTGGTATGGC
GCAGCTACTGCTAGAAATAGAGCTATTATAGTAGCAGTTAAAAAAGAAATAAAAAAAATTTTTACCTTTCCTAAAATCAC
TCATTTTCAAAAAGATAATCCATTAGGGAATATTAATGGAATGAGTTTTTGTGATAATTTACTCCCTTATACTACTGTAG
AAGATGTTTTCAAAACCATTGATTATAATAATTTAGATGATTTAGATAATATACCGATGTCACACAATCCCAAAACTATT
AAAAGATTTGGTTTTATCCCTGAGGGAAAAAATATTTCTCATATTATTGACCAATTACCAAATGAACTTAAAATTAGTAG
TTTTTATTCTCGTGGAAGTGCTACTAGATTATCTAGACACAAACCATGCGGCACTTTGGTTCCAGGACATAGTGCTTTAC
CTATCCATCCTTGGTTGAATAGAAGCATCACAATAAGAGAAGCCGCTGCTTTAAGTGGATTTCCATTAAATTATAAATTT
TATGGGAGCCATTCAAATAGGTGTGAACAAATAGGTAACGCAGTACCTATTTTACTATCTAATGCTATTGCTAAAAATAT
AAAAAAATTTTTAAAAGAGGTAAAAAGTGAATGA

Upstream 100 bases:

>100_bases
TTTAAAACAATTAGAATTTGTTAACCATTTAAAACCTACAATTTCTATTATTGAAAATGTTCCTACTTTTTTAACTACTA
AAAACTTAAAAAAGAAATTG

Downstream 100 bases:

>100_bases
AAACAAATTAACGGATTTAATTCTAAAAGATGATAATTTTAAAAAAAATTTTGCTCGTTTATTAAATATCGATGATTTTA
TAATTCAAAAAGAAGAAAAA

Product: Putative C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.Sau96I; Cytosine-specific methyltransferase Sau96I [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MIFGKNTLNFKPKKGSKRKKNLSHELENKRLLELKKQKNDLLKKLEKQQVLISVFDDIKNKYDKMGYKVYHQVLNSAWYG
AATARNRAIIVAVKKEIKKIFTFPKITHFQKDNPLGNINGMSFCDNLLPYTTVEDVFKTIDYNNLDDLDNIPMSHNPKTI
KRFGFIPEGKNISHIIDQLPNELKISSFYSRGSATRLSRHKPCGTLVPGHSALPIHPWLNRSITIREAAALSGFPLNYKF
YGSHSNRCEQIGNAVPILLSNAIAKNIKKFLKEVKSE

Sequences:

>Translated_277_residues
MIFGKNTLNFKPKKGSKRKKNLSHELENKRLLELKKQKNDLLKKLEKQQVLISVFDDIKNKYDKMGYKVYHQVLNSAWYG
AATARNRAIIVAVKKEIKKIFTFPKITHFQKDNPLGNINGMSFCDNLLPYTTVEDVFKTIDYNNLDDLDNIPMSHNPKTI
KRFGFIPEGKNISHIIDQLPNELKISSFYSRGSATRLSRHKPCGTLVPGHSALPIHPWLNRSITIREAAALSGFPLNYKF
YGSHSNRCEQIGNAVPILLSNAIAKNIKKFLKEVKSE
>Mature_277_residues
MIFGKNTLNFKPKKGSKRKKNLSHELENKRLLELKKQKNDLLKKLEKQQVLISVFDDIKNKYDKMGYKVYHQVLNSAWYG
AATARNRAIIVAVKKEIKKIFTFPKITHFQKDNPLGNINGMSFCDNLLPYTTVEDVFKTIDYNNLDDLDNIPMSHNPKTI
KRFGFIPEGKNISHIIDQLPNELKISSFYSRGSATRLSRHKPCGTLVPGHSALPIHPWLNRSITIREAAALSGFPLNYKF
YGSHSNRCEQIGNAVPILLSNAIAKNIKKFLKEVKSE

Specific function: This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the Sau96I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 31668; Mature: 31668

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFGKNTLNFKPKKGSKRKKNLSHELENKRLLELKKQKNDLLKKLEKQQVLISVFDDIKN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYDKMGYKVYHQVLNSAWYGAATARNRAIIVAVKKEIKKIFTFPKITHFQKDNPLGNING
HHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCH
MSFCDNLLPYTTVEDVFKTIDYNNLDDLDNIPMSHNPKTIKRFGFIPEGKNISHIIDQLP
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHCC
NELKISSFYSRGSATRLSRHKPCGTLVPGHSALPIHPWLNRSITIREAAALSGFPLNYKF
CHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEEEEHHHHHCCCCCCEEE
YGSHSNRCEQIGNAVPILLSNAIAKNIKKFLKEVKSE
ECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFGKNTLNFKPKKGSKRKKNLSHELENKRLLELKKQKNDLLKKLEKQQVLISVFDDIKN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYDKMGYKVYHQVLNSAWYGAATARNRAIIVAVKKEIKKIFTFPKITHFQKDNPLGNING
HHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCH
MSFCDNLLPYTTVEDVFKTIDYNNLDDLDNIPMSHNPKTIKRFGFIPEGKNISHIIDQLP
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHCC
NELKISSFYSRGSATRLSRHKPCGTLVPGHSALPIHPWLNRSITIREAAALSGFPLNYKF
CHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEEEEHHHHHCCCCCCEEE
YGSHSNRCEQIGNAVPILLSNAIAKNIKKFLKEVKSE
ECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2204026 [H]