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The map label for this gene is dnaN [H]

Identifier: 197294171

GI number: 197294171

Start: 1418

End: 2554

Strand: Direct

Name: dnaN [H]

Synonym: PAa_0002

Alternate gene names: 197294171

Gene position: 1418-2554 (Clockwise)

Preceding gene: 197294170

Following gene: 197294172

Centisome position: 0.16

GC content: 21.64

Gene sequence:

>1137_bases
ATGTATTTAGAAATTAATAAAGATGTTTTTTTAAAACAACTTTTAAAAATATATAAAATTTTACCACAGAAAAGTTTATT
TCCTGTTTTCAACATATTAAAAATCACTGCTAAAAAAAACACTTTAATTTTAGAAGTTAACAATGGGAATATTGCGATTC
AAATCTCGATTGAAGATCAAAGTTTGAAAATTGAAAAAGAAGGAGAAATCGCTTGTTTTGGTAGACATTTTATTGAAATA
ATTAAAAAAATTAACACCCTTTCAATCAAAATGACTGTTACAGAAAATAATTTTTTAGTGATTAAAACTAATTTTTGTGA
ATATAAATTAAAATTAATGACTCTTTTAGGGTTTTTATTGCCTGAATTTTTTTTCGAAAAAAAAGAATTTTTTGAATTAA
AGGTTTCTTCTTTTAAAAAAATGATTAAAGAAGTTAATATTTCTAGTTCTAAAAATGAAAAAAGACCTATTTTAACAGGT
TCTAATCTAATTTATGAAAAAAAGACCTTAAAAGCTTTGGCAACCGATTCTTTTCGAATAAGTTATAAAAAAATTAAATT
AGATTTTCCTTATTTTGATTTTAATATGGTTATTCCTAACAAAAGTTTAGAGGAATTAATTAAAATATTAGAATATTACG
ATAACAAAAACTTAAAAATTTATTCAGATGATAAAAAAATTTTTTTAAAAATCGATAATTTATGGTTTTGCACTTCTCTT
TTGGAAGGAAATTATCCTCAAATTCAAGAATTTAATTTAAAAAATTATCCCTTTGTTGTTCATTTAAATAAAGATGATGT
AATGAAAGTTTTAGAAAGAATTTCTTTATTTTTTTCAAAAGAAGAAGTAAATAAAAATATTGTTTTGTTTAAATTAACTG
AGGAAAAAATAATTGAAATATCAATTTCCAATGAAAATTTAGGAACAGCTTTAGAAAAAATCATCCCTTTAAAAGTAACT
TCAGAAAAATTTGAAATAGCTTTTAATGCCAAGCATTTGGAAGATATTTTAAAAGTTTTGACTTCTTCTGAAATTGTTTT
TTATTTTGAAAGTCACCTCAAACCTTTTATTGTTACTACTTTAGAAGAAGAAAGTTCAGTTCATTTGATTTTTCCTATTC
AGAAAGAAAATGACTGA

Upstream 100 bases:

>100_bases
CACGAATTAAAAAAATCAATTGAACTAATTTTAGAAAAAATAAATAATTAACTTTTAACTTTTTAAAAAAACCAATTCAG
GAAGGAAATAATTTATTTCA

Downstream 100 bases:

>100_bases
TTTTTTACTATTTTTTAACCCCGTTTTTTTAAATGTTGAGAAAAAATAATTTGAGTACTGCTGTCTTTTTGTGATAAACG
CCCTTTTTGAAGGGCGTAAT

Product: DNA polymerase III beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MYLEINKDVFLKQLLKIYKILPQKSLFPVFNILKITAKKNTLILEVNNGNIAIQISIEDQSLKIEKEGEIACFGRHFIEI
IKKINTLSIKMTVTENNFLVIKTNFCEYKLKLMTLLGFLLPEFFFEKKEFFELKVSSFKKMIKEVNISSSKNEKRPILTG
SNLIYEKKTLKALATDSFRISYKKIKLDFPYFDFNMVIPNKSLEELIKILEYYDNKNLKIYSDDKKIFLKIDNLWFCTSL
LEGNYPQIQEFNLKNYPFVVHLNKDDVMKVLERISLFFSKEEVNKNIVLFKLTEEKIIEISISNENLGTALEKIIPLKVT
SEKFEIAFNAKHLEDILKVLTSSEIVFYFESHLKPFIVTTLEEESSVHLIFPIQKEND

Sequences:

>Translated_378_residues
MYLEINKDVFLKQLLKIYKILPQKSLFPVFNILKITAKKNTLILEVNNGNIAIQISIEDQSLKIEKEGEIACFGRHFIEI
IKKINTLSIKMTVTENNFLVIKTNFCEYKLKLMTLLGFLLPEFFFEKKEFFELKVSSFKKMIKEVNISSSKNEKRPILTG
SNLIYEKKTLKALATDSFRISYKKIKLDFPYFDFNMVIPNKSLEELIKILEYYDNKNLKIYSDDKKIFLKIDNLWFCTSL
LEGNYPQIQEFNLKNYPFVVHLNKDDVMKVLERISLFFSKEEVNKNIVLFKLTEEKIIEISISNENLGTALEKIIPLKVT
SEKFEIAFNAKHLEDILKVLTSSEIVFYFESHLKPFIVTTLEEESSVHLIFPIQKEND
>Mature_378_residues
MYLEINKDVFLKQLLKIYKILPQKSLFPVFNILKITAKKNTLILEVNNGNIAIQISIEDQSLKIEKEGEIACFGRHFIEI
IKKINTLSIKMTVTENNFLVIKTNFCEYKLKLMTLLGFLLPEFFFEKKEFFELKVSSFKKMIKEVNISSSKNEKRPILTG
SNLIYEKKTLKALATDSFRISYKKIKLDFPYFDFNMVIPNKSLEELIKILEYYDNKNLKIYSDDKKIFLKIDNLWFCTSL
LEGNYPQIQEFNLKNYPFVVHLNKDDVMKVLERISLFFSKEEVNKNIVLFKLTEEKIIEISISNENLGTALEKIIPLKVT
SEKFEIAFNAKHLEDILKVLTSSEIVFYFESHLKPFIVTTLEEESSVHLIFPIQKEND

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=17.8666666666667, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44390; Mature: 44390

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLEINKDVFLKQLLKIYKILPQKSLFPVFNILKITAKKNTLILEVNNGNIAIQISIEDQ
CEEEECHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEECCCEEEEEECCCCEEEEEEEECC
SLKIEKEGEIACFGRHFIEIIKKINTLSIKMTVTENNFLVIKTNFCEYKLKLMTLLGFLL
EEEEECCCCEEEEHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHH
PEFFFEKKEFFELKVSSFKKMIKEVNISSSKNEKRPILTGSNLIYEKKTLKALATDSFRI
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEHHHHHHHCCCCEEE
SYKKIKLDFPYFDFNMVIPNKSLEELIKILEYYDNKNLKIYSDDKKIFLKIDNLWFCTSL
EEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCEEEEEHH
LEGNYPQIQEFNLKNYPFVVHLNKDDVMKVLERISLFFSKEEVNKNIVLFKLTEEKIIEI
HCCCCCCCEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEE
SISNENLGTALEKIIPLKVTSEKFEIAFNAKHLEDILKVLTSSEIVFYFESHLKPFIVTT
EECCCCHHHHHHHHEEEEEECCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCEEEEEE
LEEESSVHLIFPIQKEND
ECCCCCEEEEEEEECCCC
>Mature Secondary Structure
MYLEINKDVFLKQLLKIYKILPQKSLFPVFNILKITAKKNTLILEVNNGNIAIQISIEDQ
CEEEECHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEECCCEEEEEECCCCEEEEEEEECC
SLKIEKEGEIACFGRHFIEIIKKINTLSIKMTVTENNFLVIKTNFCEYKLKLMTLLGFLL
EEEEECCCCEEEEHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHH
PEFFFEKKEFFELKVSSFKKMIKEVNISSSKNEKRPILTGSNLIYEKKTLKALATDSFRI
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEHHHHHHHCCCCEEE
SYKKIKLDFPYFDFNMVIPNKSLEELIKILEYYDNKNLKIYSDDKKIFLKIDNLWFCTSL
EEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCEEEEEHH
LEGNYPQIQEFNLKNYPFVVHLNKDDVMKVLERISLFFSKEEVNKNIVLFKLTEEKIIEI
HCCCCCCCEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEE
SISNENLGTALEKIIPLKVTSEKFEIAFNAKHLEDILKVLTSSEIVFYFESHLKPFIVTT
EECCCCHHHHHHHHEEEEEECCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCEEEEEE
LEEESSVHLIFPIQKEND
ECCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA