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The map label for this gene is fruK [H]

Identifier: 170757758

GI number: 170757758

Start: 2137985

End: 2138908

Strand: Reverse

Name: fruK [H]

Synonym: CLD_2632

Alternate gene names: 170757758

Gene position: 2138908-2137985 (Counterclockwise)

Preceding gene: 170755100

Following gene: 170756738

Centisome position: 54.04

GC content: 28.46

Gene sequence:

>924_bases
ATGATTTATACATTAACTACTAATCCAGCTATTGATATGAACTGCTCTTCCAGTAGTAATGAAGCTTTTAAGGTAAATAG
AACCAATAATTTATCTTATTCACCTAATGGAAAAGGAGTAAACGTATCACTAGTTTTAAATCATTATTCTATAGAGAGTA
AAGCTCTTGGATTTTTCGGAGGATTTACAGGAAAGTATATTGTAGAGGAATTAAATAAGAAAAATATACTTACAGAAGCC
TTTTGGATAGAAGAAAATACAAGGATAAATGTATTTATAAATACGGAAGATAATAAAGAATTTAAGTATGTAAACAAGGG
ACCTTTTGTTTCAAGGGATACCCAAAAGGATCTATTAAAATACTTATCATCAAATTTTAAAAAAGGTGATTATTTAATAA
TAAGTGGAAGTCTTCCTCAAAGTATTCAAGAGGATTACTACAATGAAATTTTAGATATATGTATGGAAAATAATGTTCAA
GTTATATTAGATATAAGCTCTAAAAAATTAAAAGAATTATTGAAATATAAACCTTTATTAATTAAACCTAATGATGAAGA
AGTTGAGGCTATATTTGGACTAAGTTTAAAAGATGAAGAGGATATAAAAGAGGCATTAAAATATATTTATAAGTTAGGTG
CTAAAAATATACTTCTAACCTTAGGGGAGAAAGGAATGTATTTTTACAATGGAGACAAGATTTATTATTGCAATGCACCT
AAAATTAAACTTTTAAGCTCTGCTTGTGCAGGGGACTCGGCCTTAGCAGCATTTTTAAGTGAATGGTTATATGAATCTAA
TATAGAAAAGGCACTTAAAAAGGCATCAGCCACTGGTGCAAATGTTGCTGAATCAAGTGCATTAGGAACTTTTGGTAAAG
TATCGGAGTATATAAAAAAAATACATGTTAAGGAGGTAGTGTAA

Upstream 100 bases:

>100_bases
TATATGACACCAGGAAAAGAAGTTATGAGAGAAGTAGTAAGGCATAAAATAATGGTTTGTGGAAGTAATGGACAAGCTTA
GACCTAGAGGAGATGACGCT

Downstream 100 bases:

>100_bases
ATGAAAAAGATTATTGCTGTTACAGGATGCCCTACAGGAATAGCTCATACATTTATGGCAGAAGAAGCATTAAAGAAAGC
AGCTAAAGAATTAGGAGTAG

Product: PfkB family kinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIYTLTTNPAIDMNCSSSSNEAFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFGGFTGKYIVEELNKKNILTEA
FWIEENTRINVFINTEDNKEFKYVNKGPFVSRDTQKDLLKYLSSNFKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQ
VILDISSKKLKELLKYKPLLIKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
KIKLLSSACAGDSALAAFLSEWLYESNIEKALKKASATGANVAESSALGTFGKVSEYIKKIHVKEVV

Sequences:

>Translated_307_residues
MIYTLTTNPAIDMNCSSSSNEAFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFGGFTGKYIVEELNKKNILTEA
FWIEENTRINVFINTEDNKEFKYVNKGPFVSRDTQKDLLKYLSSNFKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQ
VILDISSKKLKELLKYKPLLIKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
KIKLLSSACAGDSALAAFLSEWLYESNIEKALKKASATGANVAESSALGTFGKVSEYIKKIHVKEVV
>Mature_307_residues
MIYTLTTNPAIDMNCSSSSNEAFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFGGFTGKYIVEELNKKNILTEA
FWIEENTRINVFINTEDNKEFKYVNKGPFVSRDTQKDLLKYLSSNFKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQ
VILDISSKKLKELLKYKPLLIKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
KIKLLSSACAGDSALAAFLSEWLYESNIEKALKKASATGANVAESSALGTFGKVSEYIKKIHVKEVV

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=282, Percent_Identity=25.177304964539, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788493, Length=280, Percent_Identity=27.1428571428571, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 34511; Mature: 34511

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTLTTNPAIDMNCSSSSNEAFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFG
CEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHC
GFTGKYIVEELNKKNILTEAFWIEENTRINVFINTEDNKEFKYVNKGPFVSRDTQKDLLK
CCCHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHH
YLSSNFKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQVILDISSKKLKELLKYKPLL
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCEE
IKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
ECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCEEEEECCC
KIKLLSSACAGDSALAAFLSEWLYESNIEKALKKASATGANVAESSALGTFGKVSEYIKK
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHH
IHVKEVV
HHHHHCC
>Mature Secondary Structure
MIYTLTTNPAIDMNCSSSSNEAFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFG
CEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHC
GFTGKYIVEELNKKNILTEAFWIEENTRINVFINTEDNKEFKYVNKGPFVSRDTQKDLLK
CCCHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHH
YLSSNFKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQVILDISSKKLKELLKYKPLL
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCEE
IKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
ECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCEEEEECCC
KIKLLSSACAGDSALAAFLSEWLYESNIEKALKKASATGANVAESSALGTFGKVSEYIKK
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHH
IHVKEVV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]