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The map label for this gene is tilS

Identifier: 170757599

GI number: 170757599

Start: 3840966

End: 3842372

Strand: Reverse

Name: tilS

Synonym: CLD_0964

Alternate gene names: 170757599

Gene position: 3842372-3840966 (Counterclockwise)

Preceding gene: 170756604

Following gene: 170754732

Centisome position: 97.07

GC content: 24.31

Gene sequence:

>1407_bases
ATGAAAGATGTGGTTATAAATACTATAAAAAAATATAATATGATTGAAGCTAATGACAAAATAATAGTAGGAGTATCCGG
CGGACCAGATTCTATGTGTCTTTTACATATGTTATGTTTATTAAAAAATCAATTAAGAATAAAAGATATATATGTGGCTC
ATATAAATCATGGTGTTAGAGGAGCAGAATCAGATGCTGATGAAAAATATGTAGAGAATTTTTGTTATACGAATAATTTA
GGTTTTTTTTCCAAAACAATAAATATGGATAAGATAGCTAAAGAAAAAGGAATTTCCAGTGAAAGTGCAGGAAGAGAAGC
AAGATATGATTTTTTTAACTATTTGAGAAAAGAACTAGGTGCTCAAAAAATAGCAACAGCTCATAATGCAAATGATCAAG
CAGAAACTGTACTTATGAGAATAATGCGAGGGACAGGATTACAGGGATTAATAGGTATAAACCCAATAAGAGATGATTTA
TATATAAGACCTTTAATAAATGTATTAAGAGAAGATATAGAAAATTATTGTGATAAATATGAGCTAAATCCTAGAATAGA
TAAGACAAATTTACAAAGCATATATACAAGAAATAAAATTAGATTAGAATTGATACCTTATATAAAAGATAATTTTAATG
AAGATATAGTAAATACTTTATGTAGATTTTCTAATATAGTTTCTAAAGACAATAACTATTTAGAAGAAGTATCAAAAGAT
AAATTTGAAAGATATTGTACTAAAAAATTACAAAGGGTAATAATAGATAAGAGAGCTTTTTTAGAGCATGAAAGTATTTC
AACTAGAATATTAAGAGAGGCTATACTTTATATAAACCAAAACTTATATAATCTTGAAATGAGGAATATATATGATGTTT
TAGAATTAAGCTTAAATGCAACAGGTAAATTTGTAAACTTGCCTAGCAATATAAAAGCTGAAAATATTTATGGAGATATA
CATTTATATAAAGAAGATAATTGTATAAAAAAGGTAAATAATATTCAGTGTGAACTTAAAATAGGATTTAATCAAGTAGA
AGATTTAAATATTAATTTAAAAATATATTCTATAAAAGATCATAATTATAATATAAATGATAATAAATATATACAGTATT
TTGATTATGATAAAGTAAGTAATGAAAAAATTTATTTAAGAAATAGAAAAAATGGAGATAAGTTTACTCCTTTAGGGATG
AAAGGTAGTAAAAAATTAAAAGATTTTTTTATAGATTCAAAAATACCCAGAGAGCAAAGAGATAAGTTAAAACTTGTATG
TTTTGGGGAAGAAATAGCTTGGATTATAGGTTGTAGAACAGGCAATAATTTTAAAATAGATAAAAATACAAAGAATGTAT
TAGAAATAACTGTTGAAAGAGGGGAAGCAAATGGATTTGATAAGTAG

Upstream 100 bases:

>100_bases
AAATATTCTCTTTTTTTTATATAAAACCCTGCTAAAAGTATAATGATTAGAGTATTTTTATGGTATAATGTTTTAATATA
AAGGTATAAGGTGTGTTTTT

Downstream 100 bases:

>100_bases
GGACATAGAAAAAATACTTATATCTGAAGAACAAATACAAAGCAAAATAAATGAATTAGGAGATCAAATCAGTAAGGATT
ATGATGGTAAAAATTTAATG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVRGAESDADEKYVENFCYTNNL
GFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELGAQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDL
YIRPLINVLREDIENYCDKYELNPRIDKTNLQSIYTRNKIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNATGKFVNLPSNIKAENIYGDI
HLYKEDNCIKKVNNIQCELKIGFNQVEDLNINLKIYSIKDHNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGM
KGSKKLKDFFIDSKIPREQRDKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK

Sequences:

>Translated_468_residues
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVRGAESDADEKYVENFCYTNNL
GFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELGAQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDL
YIRPLINVLREDIENYCDKYELNPRIDKTNLQSIYTRNKIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNATGKFVNLPSNIKAENIYGDI
HLYKEDNCIKKVNNIQCELKIGFNQVEDLNINLKIYSIKDHNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGM
KGSKKLKDFFIDSKIPREQRDKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK
>Mature_468_residues
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVRGAESDADEKYVENFCYTNNL
GFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELGAQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDL
YIRPLINVLREDIENYCDKYELNPRIDKTNLQSIYTRNKIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNATGKFVNLPSNIKAENIYGDI
HLYKEDNCIKKVNNIQCELKIGFNQVEDLNINLKIYSIKDHNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGM
KGSKKLKDFFIDSKIPREQRDKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=200, Percent_Identity=30, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54504; Mature: 54504

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVR
CCCHHHHHHHHCCEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCC
GAESDADEKYVENFCYTNNLGFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELG
CCCCCHHHHHHHHHHEECCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
AQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDLYIRPLINVLREDIENYCDKY
HHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ELNPRIDKTNLQSIYTRNKIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
CCCCCCCCCHHHHHHHCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCC
TGKFVNLPSNIKAENIYGDIHLYKEDNCIKKVNNIQCELKIGFNQVEDLNINLKIYSIKD
CCCEEECCCCCCCCCEEEEEEEEECCCHHHHHCCEEEEEEECCCCEEEEEEEEEEEEEEC
HNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGMKGSKKLKDFFIDSKIPREQR
CCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
DKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK
CCEEEEEECCCEEEEEEEECCCCEEEECCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVR
CCCHHHHHHHHCCEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCC
GAESDADEKYVENFCYTNNLGFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELG
CCCCCHHHHHHHHHHEECCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
AQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDLYIRPLINVLREDIENYCDKY
HHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ELNPRIDKTNLQSIYTRNKIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
CCCCCCCCCHHHHHHHCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCC
TGKFVNLPSNIKAENIYGDIHLYKEDNCIKKVNNIQCELKIGFNQVEDLNINLKIYSIKD
CCCEEECCCCCCCCCEEEEEEEEECCCHHHHHCCEEEEEEECCCCEEEEEEEEEEEEEEC
HNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGMKGSKKLKDFFIDSKIPREQR
CCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
DKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK
CCEEEEEECCCEEEEEEEECCCCEEEECCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]