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The map label for this gene is sbcD [H]

Identifier: 170757430

GI number: 170757430

Start: 586431

End: 587660

Strand: Direct

Name: sbcD [H]

Synonym: CLD_0272

Alternate gene names: 170757430

Gene position: 586431-587660 (Clockwise)

Preceding gene: 170756890

Following gene: 170755486

Centisome position: 14.82

GC content: 30.89

Gene sequence:

>1230_bases
ATGAGAATATTGCATACCTCCGATTGGCACTTGGGAAAGAACCTTGAAGGCTTTAGTAGAATGGATGAACAGGAAAGATT
TATAGAGGATTTTATTAACATTGTGGATAAAAATAATATAGATATGGTGATAATAGCTGGAGATATATACGATAACGGAA
ATCCATCGGCTAGAGCAGAAAAAATGTTTTATGATTGTATAAAAAAAGTAAGCAATAAGGGAGAAAGATTAGTTTTAATT
ATAGCAGGTAATCACGATAATCCAGAAAGATTAGTTGCTGCAAGTTCTTTAGCCTATGATGAGGGAATAATACTTATGGG
GACTCCTAAGACTATAGTTCCAAAGGGAAAGTATGGGAATTTTAAGGTAATAAATTCAGATTTAGCCTGTTTAGAAATAG
AGGTAAAGGAAGAAAAGGCAGTGATTATATCTTTACCTTATCCTAGTGAGAAAAGATTAAATGAAATACTGTCTAAAGAA
ATGGATAATGATGAGGATAGAAGAAAAACTTATTCAGAAAGGATAGGAGATATATTTTCTAATTTACAGGAAAAATATTA
TAAAGATAACACTGTTAATTTAGCTATTTCTCATATATTTGTAAATGGATCAGAGGAAACAGATTCTGAAAGGCCAATAC
AACTTGGAGGGAGTCTAGCAGTAGATGTAGACAAGTTACCTAAAAAAGCCCAATATATAGGTCTTGGGCATCTGCATAAA
CCACAAAAGGTAAGGGGGATAGATAATGCCTATTATTCTGGCTCACCACTGCCTTATAGTAAAAGTGAAAGAGGATATAG
TAAAGGATGCAAGATAATAGAGGCACATCCAGGGAAAAAAGCTATTATTGAAGAGGTTTATTTTAATAACTATAAACCTA
TAGAGGTATGGCATTGCAACGGTATAAAAGAGGCTATAAATAAATGTGAAGAAAATAAAGAAAAGGATATGTGGCTATAT
TTACATATAAAAACAAAGGAATATATAAGTACAGAAGATATAAAAGCCTTAAAAGAAATAAAAAAAGATATAGTAGAGAT
AGTACCAGAAATAGAGGAAAAAAGAGAAGAAACTTTTAATGGAAATATAAAAGAAAAATCTATGGCAGAATTATTTAAAT
CCTTTTATTTAAATCAAAGAGGAATAGAGGCTAGTGAAGAATTAATGGATTTATTTTTAAGTATAGCTCAAGAGGAGGAT
GAATATGAAACCGAAGAAACTTGTGATTAA

Upstream 100 bases:

>100_bases
GATAACAATATAATTTATTTTTATATAGATTGTGAGTAGAATAAGCAAAATAATTTAATAAGATGGAAAGTTAACTAGAT
ACAAAAGGGAGATGAAGATC

Downstream 100 bases:

>100_bases
AGGGCTAAACAGTTTTATAGAAAAACAGGAAATAGACTTTGAAACTTTAGTCAATAGAGGTTTGTTTGGCATATTTGGCC
CTACAGGCAGTGGAAAATCC

Product: putative nuclease SbcCD, D subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPSARAEKMFYDCIKKVSNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGKKAIIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EYETEETCD

Sequences:

>Translated_409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPSARAEKMFYDCIKKVSNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGKKAIIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EYETEETCD
>Mature_409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPSARAEKMFYDCIKKVSNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGKKAIIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EYETEETCD

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=393, Percent_Identity=26.972010178117, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46974; Mature: 46974

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPSARAE
CEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
KMFYDCIKKVSNKGERLVLIIAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGN
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCC
FKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKEMDNDEDRRKTYSERIGDIFS
EEEECCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
HHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEECCCEEEEHHHCCCHHHEECCCCCCC
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGKKAIIEEVYFNNYKPIEVWHCN
CHHHCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCCCEEEEECC
GIKEAINKCEENKEKDMWLYLHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFN
CHHHHHHHHHCCCCCCEEEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEEDEYETEETCD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPSARAE
CEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
KMFYDCIKKVSNKGERLVLIIAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGN
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCC
FKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKEMDNDEDRRKTYSERIGDIFS
EEEECCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
HHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEECCCEEEEHHHCCCHHHEECCCCCCC
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGKKAIIEEVYFNNYKPIEVWHCN
CHHHCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCCCEEEEECC
GIKEAINKCEENKEKDMWLYLHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFN
CHHHHHHHHHCCCCCCEEEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEEDEYETEETCD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]