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The map label for this gene is ubiE [C]

Identifier: 170756890

GI number: 170756890

Start: 584396

End: 584986

Strand: Direct

Name: ubiE [C]

Synonym: CLD_0275

Alternate gene names: 170756890

Gene position: 584396-584986 (Clockwise)

Preceding gene: 170755014

Following gene: 170757430

Centisome position: 14.76

GC content: 26.23

Gene sequence:

>591_bases
ATGAATGATAAAGAGTTTTTTAATAATTTAGCAGATAAGTGGGACGATATGTGTTTTCATTCAAAAGAGAAAATAGAATA
TATTCTTTCAAAAGCAAATTTGAAAGAAGGAGATAGAGTTTTAGATATAGGTAGTGGTACAGGAGTACTTATACCTTATT
TAGAAAATATTATTTCTAATAATGGTAATATAGCTGCTATAGATATAGCAGAAAATATGTTAAAGGTATCAAAAGAAAAA
AACAAATACTCTAATTTAAAGTTTATAGTAGGAGATTTTTTAGAGTATAAATCTAAAAAAACTTTTAATTGCATAACAGC
ATATTCTTGTTATCCTCATTTTAAGGACAAGGATAAATTAGCACATAGAGCCTATGAACTACTCGAGGAAGGTGGTAGAT
TAGTTATAGCTCATAGTGAAAGTAAAGAGAAGATAAACTCTAGACATAAAGATGTAGATCATAAGATAAAGTCACATATA
TTGCCTAAGGTGAAATATACTGCAGAATTAATGGAAAAACATAATTTTAAAACTATATATACAGAGGATAATGAAGAATG
TTATATTTATATAGGTACAAGAAACAAATAA

Upstream 100 bases:

>100_bases
TTATTTGTAAAAGGTTTGCCGGGTATAATTATTCAATTAATACTTATACCTATATTAGTTAAATCATTAGGGAAAGTAGA
ATTAGGAAAGGCTGTATAAT

Downstream 100 bases:

>100_bases
TTTGATTAGATAAATTTTATGTATGATTAACCAAAATAAAAGTGTGGAGTAAAAATATAAAAACTACTCCACACTTTTAC
TTTATTTAAATTAATTCATC

Product: methlytransferase,-like protein

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: Menaquinone Biosynthesis Methyltransferase UbiE; SAM-Dependent Methyltransferase; Methyltransferase Family Protein; Methyltransferase Domain Protein; Methyltransferase With A HTH Motif; Methyltransferase UbiE/COQ5 Family; Methlytransferase; UbiE/COQ5 Methyltransferase

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNGNIAAIDIAENMLKVSKEK
NKYSNLKFIVGDFLEYKSKKTFNCITAYSCYPHFKDKDKLAHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHI
LPKVKYTAELMEKHNFKTIYTEDNEECYIYIGTRNK

Sequences:

>Translated_196_residues
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNGNIAAIDIAENMLKVSKEK
NKYSNLKFIVGDFLEYKSKKTFNCITAYSCYPHFKDKDKLAHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHI
LPKVKYTAELMEKHNFKTIYTEDNEECYIYIGTRNK
>Mature_196_residues
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNGNIAAIDIAENMLKVSKEK
NKYSNLKFIVGDFLEYKSKKTFNCITAYSCYPHFKDKDKLAHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHI
LPKVKYTAELMEKHNFKTIYTEDNEECYIYIGTRNK

Specific function: Methyltransferase Required For The Conversion Of Dimethylmenaquinone (Dmkh2) To Menaquinone (Mkh2) And The Conversion Of 2-Polyprenyl-6-Methoxy-1,4-Benzoquinol (Ddmqh2) To 2-Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol (Dmqh2). [C]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994983, Length=191, Percent_Identity=27.7486910994764, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.- [C]

Molecular weight: Translated: 22795; Mature: 22795

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISN
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCC
NGNIAAIDIAENMLKVSKEKNKYSNLKFIVGDFLEYKSKKTFNCITAYSCYPHFKDKDKL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
AHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHILPKVKYTAELMEKHNFKTIY
HHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
TEDNEECYIYIGTRNK
ECCCCEEEEEEECCCC
>Mature Secondary Structure
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISN
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCC
NGNIAAIDIAENMLKVSKEKNKYSNLKFIVGDFLEYKSKKTFNCITAYSCYPHFKDKDKL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
AHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHILPKVKYTAELMEKHNFKTIY
HHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
TEDNEECYIYIGTRNK
ECCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA