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The map label for this gene is rumA

Identifier: 170757395

GI number: 170757395

Start: 3088365

End: 3089741

Strand: Reverse

Name: rumA

Synonym: CLD_1683

Alternate gene names: 170757395

Gene position: 3089741-3088365 (Counterclockwise)

Preceding gene: 170757633

Following gene: 170757814

Centisome position: 78.06

GC content: 29.99

Gene sequence:

>1377_bases
ATGAAAAATAAATATATGAGAAAAGGCAAGGAGTATGAATTTCTTATAGAAGAAACAGAATTTCCAGGAACAGGTGTAGC
ACAAAAGGATGGTGTGCCAGTTTATATAAAAGGAACAGTACCAGGACAAAAGGTTTTAGCAAAAGTAACTAAAAAAAGAA
GAGAATATGCTCAGGCTAAACTTTTAGAAATAATAGAAAATATAGATTATGCAATAGAAAATAAATGTCCTCATTTTGGT
CAATGTGGAGGATGTTCAACCCAATACATACCTTATGAAAAACAACTACAAATTAAAGAAGAACAGTTGTTAAAATTATT
TAAAAGTAAAGAAATAAAAGGATTTGATTTTTTAGGAGTAGAAAAAAGTCCAGAGGAATATGAATATAGAAATAAGATGG
AGTTTACCTTTGGGGATATGGAAAAGGGCGGAGATCTTACTTTAGGAATGCATGTTAAAAATAGAAATTTTTCTATTGTA
ACTGTGGATAAGTGTGAAATTGTAGATAGGGATTTTAGAAATATATTAACAACTGTGGTTAATTATTTTAATGAAAAAAG
ATTACCTAAATACAGAGTAATGAGTCATGAAGGTTTTTTAAGAAACTTAGTTATAAGAAAAGCTAAAAATACTGGAGAAA
TATTAATAAACATAGTAACTACATCTCAAATGGAATTTGATTTTAAAGAAATAGTAGATATGTTATTAAAGGTGGAATGC
AAAGGAGAAATAAAAGGTATTCTTCATACTATAAATGACACATTATCTGATGTAGTTCAGGTGGATAAATTAGAGATATT
ATATGGAAGAGACTATATAATAGAAGAGTTATTAGGACTAAAATTTAAGATAGCTCCAGAAGCCTTTTTTCAAACAAATT
CAAAGGGAGCAGAAAAACTATATAGTATAGTAAAAGACTTCTTAGGTGATGCTAGTTCAAAAGTGGTTTTTGACCTTTAT
TGTGGTACAGGAACCATAGGACAAATAGTTGCTCCAAAGGCTAAAAAGGTTATAGGTGTGGAATTAATAGAAGAAGCAGT
TAAGGCAGCTAATGAAAATGCAAAATTAAATGGACTAAATAACTGTGAGTTTATAGCTGGTGATGTAGCAAAGGTTATAA
AAGATGTAAAACAAAAACCAGATATAATTATATTGGATCCACCAAGACCAGGTGTACATCCAGTAGCTTTAGAATATGTG
GTTAAGTTTGAGCCAAAGGAAATAATCTATGTATCCTGTAATCCAAAGACTTTAGTAGATGATTTAAAATATCTTATAGA
TAATGGGTATAAATTAGAAAAAGTTAAGGGCATGGACATGTTTCCTCATACGCCACATGTGGAGACTTGCGTGTTGCTAC
AACGAAAAACTATATAG

Upstream 100 bases:

>100_bases
AGTCTATTATTTGTAGTAGTTACAGAAAATAGTATTAATAGTATAGCAATAAAATAAAATCTAATAAATAAAATACTTAA
AATAAATTTACTCTGAAAGG

Downstream 100 bases:

>100_bases
AAATCCTGAATTTTAGGCACTTCTTCAACCATTTTGTTTTTACAAATGAGGGAGATAAAATTCATAATAAACAGCTAAAA
AAATGTATCAATGTTTCTGT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFG
QCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIV
TVDKCEIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVEC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLY
CGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYV
VKFEPKEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETCVLLQRKTI

Sequences:

>Translated_458_residues
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFG
QCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIV
TVDKCEIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVEC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLY
CGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYV
VKFEPKEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETCVLLQRKTI
>Mature_458_residues
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFG
QCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIV
TVDKCEIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVEC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLY
CGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYV
VKFEPKEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETCVLLQRKTI

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=360, Percent_Identity=30.2777777777778, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI51173878, Length=360, Percent_Identity=30.2777777777778, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI269784680, Length=364, Percent_Identity=30.2197802197802, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI269784676, Length=364, Percent_Identity=30.2197802197802, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI20149688, Length=364, Percent_Identity=30.2197802197802, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI269784678, Length=358, Percent_Identity=30.1675977653631, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1789148, Length=447, Percent_Identity=28.1879194630872, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1787083, Length=376, Percent_Identity=25.7978723404255, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790403, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=551, Percent_Identity=25.5898366606171, Blast_Score=131, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6322909, Length=416, Percent_Identity=26.4423076923077, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24666579, Length=433, Percent_Identity=28.6374133949192, Blast_Score=148, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52155; Mature: 52155

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAK
CCCCHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LLEIIENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGV
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECC
EKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKCEIVDRDFRNILTTVV
CCCCHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHH
NYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVEC
HHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKL
CHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHH
YSIVKDFLGDASSKVVFDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLN
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCC
NCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVD
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEEEEEECCCEEEEEECCCHHHHH
DLKYLIDNGYKLEKVKGMDMFPHTPHVETCVLLQRKTI
HHHHHHCCCEEEEEECCCCCCCCCCCHHHEEHHHCCCC
>Mature Secondary Structure
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAK
CCCCHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LLEIIENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGV
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECC
EKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKCEIVDRDFRNILTTVV
CCCCHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHH
NYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVEC
HHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKL
CHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHH
YSIVKDFLGDASSKVVFDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLN
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCC
NCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVD
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEEEEEECCCEEEEEECCCHHHHH
DLKYLIDNGYKLEKVKGMDMFPHTPHVETCVLLQRKTI
HHHHHHCCCEEEEEECCCCCCCCCCCHHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]