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The map label for this gene is mspIR [H]

Identifier: 170756981

GI number: 170756981

Start: 2224196

End: 2224987

Strand: Reverse

Name: mspIR [H]

Synonym: CLD_2549

Alternate gene names: 170756981

Gene position: 2224987-2224196 (Counterclockwise)

Preceding gene: 170754579

Following gene: 170757678

Centisome position: 56.21

GC content: 26.77

Gene sequence:

>792_bases
ATGTGTGATAAAATAAAAGAAGCGTATGCAGAGTATAATATAGAAAATTTATCTTATGGAGAAATAGGAGACAAGCTAGG
AGATGCATATGAAAATTTTGTAGTTAATGTATTCAATGATGTAAATTATTTAATGAATTTTAGTCAGCTTAATTATGATA
AATTAGATGAACGTATATTTAAAAGTATATTTAAAAAAGAAGGAATAGATGTAAATCAAGTAATTAAAATCGAAGCAACT
AGTAAAATTCCTAAAAGAAATAGTGGTGGAAATTCAAAGACAGACGTGTTAGTCAAAGTTCATATAAAAAATGGACAGGT
AATAAATATACCTATTAGCGTAAAACAAACAACTGTAGCTAAGGTAGCAATGGCAGAATATGATGTTGATACAATATTAA
CTGAAACTGGCATAAATAATTCGGAAATAGAAAGATTAATGAAAAAGCATCAACAAGATGCAAGTGCAATAAATTTCTCT
AAAGAAGAAAAGCAAATTTTAAAAAATGAATTAGCAAAGAATAACAACAAAGAAAGATTATTAAGATGGATATTAACAAT
GAGTCCATTACCCAATAATGACGATATTAGAATTCCAATATATATTATTAAATTCCAATTAGATAAAAAAAGTTTAGATG
TAAAAGAGTTAGGTATTTTTAATATAGATGAATATATAAGACACATAAGTACAGATAAAAAAGGAAATCCAGCTAAAGGT
GGATTTGGAACAGGATTATCATGGACATATGCGACGGGCTCAAAGGGGAAAAAGATACAATTTAAAGGATAA

Upstream 100 bases:

>100_bases
TAGATGAAATAGATTTTTGCGACAAAAAATAAGTAATGTTATGGCAAGGCCTCCATAATATGTTGTATAATGTTTTATAT
AACAATTAGGAGGTTTTTTT

Downstream 100 bases:

>100_bases
TTCTTTTGATATTTAAAAGGAATAAATTATAAATTTTGATAAAACTATAAAATAATCAGTATATATTTTTTACAATAATA
AATTATTATTACATAAACAT

Product: type II restriction enzyme MspI (endonuclease MspI)(R.MspI)

Products: NA

Alternate protein names: R.MspI; Endonuclease MspI; Type II restriction enzyme MspI [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MCDKIKEAYAEYNIENLSYGEIGDKLGDAYENFVVNVFNDVNYLMNFSQLNYDKLDERIFKSIFKKEGIDVNQVIKIEAT
SKIPKRNSGGNSKTDVLVKVHIKNGQVINIPISVKQTTVAKVAMAEYDVDTILTETGINNSEIERLMKKHQQDASAINFS
KEEKQILKNELAKNNNKERLLRWILTMSPLPNNDDIRIPIYIIKFQLDKKSLDVKELGIFNIDEYIRHISTDKKGNPAKG
GFGTGLSWTYATGSKGKKIQFKG

Sequences:

>Translated_263_residues
MCDKIKEAYAEYNIENLSYGEIGDKLGDAYENFVVNVFNDVNYLMNFSQLNYDKLDERIFKSIFKKEGIDVNQVIKIEAT
SKIPKRNSGGNSKTDVLVKVHIKNGQVINIPISVKQTTVAKVAMAEYDVDTILTETGINNSEIERLMKKHQQDASAINFS
KEEKQILKNELAKNNNKERLLRWILTMSPLPNNDDIRIPIYIIKFQLDKKSLDVKELGIFNIDEYIRHISTDKKGNPAKG
GFGTGLSWTYATGSKGKKIQFKG
>Mature_263_residues
MCDKIKEAYAEYNIENLSYGEIGDKLGDAYENFVVNVFNDVNYLMNFSQLNYDKLDERIFKSIFKKEGIDVNQVIKIEAT
SKIPKRNSGGNSKTDVLVKVHIKNGQVINIPISVKQTTVAKVAMAEYDVDTILTETGINNSEIERLMKKHQQDASAINFS
KEEKQILKNELAKNNNKERLLRWILTMSPLPNNDDIRIPIYIIKFQLDKKSLDVKELGIFNIDEYIRHISTDKKGNPAKG
GFGTGLSWTYATGSKGKKIQFKG

Specific function: Recognizes the double-stranded sequence CCGG and cleaves after C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015291 [H]

Pfam domain/function: PF09208 Endonuc-MspI [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 29891; Mature: 29891

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDKIKEAYAEYNIENLSYGEIGDKLGDAYENFVVNVFNDVNYLMNFSQLNYDKLDERIF
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KSIFKKEGIDVNQVIKIEATSKIPKRNSGGNSKTDVLVKVHIKNGQVINIPISVKQTTVA
HHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCHHHHH
KVAMAEYDVDTILTETGINNSEIERLMKKHQQDASAINFSKEEKQILKNELAKNNNKERL
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
LRWILTMSPLPNNDDIRIPIYIIKFQLDKKSLDVKELGIFNIDEYIRHISTDKKGNPAKG
HHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCC
GFGTGLSWTYATGSKGKKIQFKG
CCCCCCEEEEECCCCCCEEEECC
>Mature Secondary Structure
MCDKIKEAYAEYNIENLSYGEIGDKLGDAYENFVVNVFNDVNYLMNFSQLNYDKLDERIF
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KSIFKKEGIDVNQVIKIEATSKIPKRNSGGNSKTDVLVKVHIKNGQVINIPISVKQTTVA
HHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCHHHHH
KVAMAEYDVDTILTETGINNSEIERLMKKHQQDASAINFSKEEKQILKNELAKNNNKERL
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
LRWILTMSPLPNNDDIRIPIYIIKFQLDKKSLDVKELGIFNIDEYIRHISTDKKGNPAKG
HHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCC
GFGTGLSWTYATGSKGKKIQFKG
CCCCCCEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2471145 [H]