| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is ddeIM [H]
Identifier: 170754579
GI number: 170754579
Start: 2225056
End: 2226390
Strand: Reverse
Name: ddeIM [H]
Synonym: CLD_2548
Alternate gene names: 170754579
Gene position: 2226390-2225056 (Counterclockwise)
Preceding gene: 170757433
Following gene: 170756981
Centisome position: 56.25
GC content: 30.04
Gene sequence:
>1335_bases ATGTACACGTATATAGATTTATTTGCTGGACCAGGTGGGTTGTGTACTGGATTCAAAAATGCAGGATTTAAACCACTGAT AGCTGTAGAAATGAGCGACAATACAGTAAAGACTTATGCTAGAAATCATGACGCAGAAATTTATAAATTAGAAGAGCTAA TTTATAACAAAGGAAAGTTAGAAAATATATTAAATATAGAGAATAATAGAACAGCTTTAATTCATGGAGATATTAGATTA GTAGATAATGATATAATTAAGGAAATATTACATAAGAAATTCAAAGTGGATAGTGTTGATGTGGTTGCAGGTGGGCCACC TTGTGAGTCTTTCTCAATGGCAGGTAAAAGAGCTGATGGAGATGAGAGAGATAACCTATTTGAAAATATGTTAAGAATAG CGCAAGCAACTAAAAGTAAATATATTTTCTTTGAAAATGTTCCTGGATTATTAACTAAAAAAAGTAATAACATTAATGTT TTTAATGTTATTGCTGATGAATTTAATAATCATGGATATAAACTAGCAAGTATAGATAAAAACATAATTAAGTGTTTAGC GTCAGATTATGGAGTGCCTCAAAATCGAGAGAGGGTATTTTTAGTAGCTATAAATGAAAAGTATGGAGATAACAAATTTA TCTATCCTGAAAAAACTCATGGAGAAGGAAGAAAATATCCTTATATAACTGTAGGAGAGGCATTTAAATATTTACCATCA TTGAATAGTGGTGGAGGGGAAGAAATTCAACAGATTCATTATTATTATGAAGAGGAATATAAGAATAATAAAATTAGTGA AGGATTTTATGAGTATATAAAATTTATATCAGGTAAAGAAGGATATATTCCAGAGCATATAAATCATGATGAATGTGTGA ATGAAATAGCTAATCATAAAGCAGTAAATCATAGAGAAAAAATGATAAAGAGATTAAGCTATATAAAACAAGGAGAAGGA ATGAAAAAAGCAGCAGAAAGATTAATAAATGAAGGGAAAGAAGATATAGTCGAGATGTATTTTCCTAAAAAATTATATGC TGCTAGAAATAGGAGATTGAAAGAAAATGAACCTTCTTTTACAGTAACTAGCCATTGTTTAGATGAAATGGTGCATCCAG AGAATGATAGAGGATTGACCCCAAGAGAAACAGCTAGATTGCAATCATTTCCAGATTGGTATGTATTTGAAGGCGATTAT GTAAAGTTTCATTCAGATCCATTACAAGATAAGTATGAGCAATGTGGTGATGCAATTCCAGTATTACTAGTAAGAGCTTT AGCTAAACAGTTGAAAATGGCATTAGATGAAATAGATTTTTGCGACAAAAAATAA
Upstream 100 bases:
>100_bases AATAGAATGTTTGTTTGATAAAGATACTAATTTATATTGGTATCTTTATTTAATTAGTATACAATATAAAATAATAATAA TTTATGAAAAGAGGTATAAT
Downstream 100 bases:
>100_bases GTAATGTTATGGCAAGGCCTCCATAATATGTTGTATAATGTTTTATATAACAATTAGGAGGTTTTTTTATGTGTGATAAA ATAAAAGAAGCGTATGCAGA
Product: DNA-cytosine methyltransferase
Products: NA
Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]
Number of amino acids: Translated: 444; Mature: 444
Protein sequence:
>444_residues MYTYIDLFAGPGGLCTGFKNAGFKPLIAVEMSDNTVKTYARNHDAEIYKLEELIYNKGKLENILNIENNRTALIHGDIRL VDNDIIKEILHKKFKVDSVDVVAGGPPCESFSMAGKRADGDERDNLFENMLRIAQATKSKYIFFENVPGLLTKKSNNINV FNVIADEFNNHGYKLASIDKNIIKCLASDYGVPQNRERVFLVAINEKYGDNKFIYPEKTHGEGRKYPYITVGEAFKYLPS LNSGGGEEIQQIHYYYEEEYKNNKISEGFYEYIKFISGKEGYIPEHINHDECVNEIANHKAVNHREKMIKRLSYIKQGEG MKKAAERLINEGKEDIVEMYFPKKLYAARNRRLKENEPSFTVTSHCLDEMVHPENDRGLTPRETARLQSFPDWYVFEGDY VKFHSDPLQDKYEQCGDAIPVLLVRALAKQLKMALDEIDFCDKK
Sequences:
>Translated_444_residues MYTYIDLFAGPGGLCTGFKNAGFKPLIAVEMSDNTVKTYARNHDAEIYKLEELIYNKGKLENILNIENNRTALIHGDIRL VDNDIIKEILHKKFKVDSVDVVAGGPPCESFSMAGKRADGDERDNLFENMLRIAQATKSKYIFFENVPGLLTKKSNNINV FNVIADEFNNHGYKLASIDKNIIKCLASDYGVPQNRERVFLVAINEKYGDNKFIYPEKTHGEGRKYPYITVGEAFKYLPS LNSGGGEEIQQIHYYYEEEYKNNKISEGFYEYIKFISGKEGYIPEHINHDECVNEIANHKAVNHREKMIKRLSYIKQGEG MKKAAERLINEGKEDIVEMYFPKKLYAARNRRLKENEPSFTVTSHCLDEMVHPENDRGLTPRETARLQSFPDWYVFEGDY VKFHSDPLQDKYEQCGDAIPVLLVRALAKQLKMALDEIDFCDKK >Mature_444_residues MYTYIDLFAGPGGLCTGFKNAGFKPLIAVEMSDNTVKTYARNHDAEIYKLEELIYNKGKLENILNIENNRTALIHGDIRL VDNDIIKEILHKKFKVDSVDVVAGGPPCESFSMAGKRADGDERDNLFENMLRIAQATKSKYIFFENVPGLLTKKSNNINV FNVIADEFNNHGYKLASIDKNIIKCLASDYGVPQNRERVFLVAINEKYGDNKFIYPEKTHGEGRKYPYITVGEAFKYLPS LNSGGGEEIQQIHYYYEEEYKNNKISEGFYEYIKFISGKEGYIPEHINHDECVNEIANHKAVNHREKMIKRLSYIKQGEG MKKAAERLINEGKEDIVEMYFPKKLYAARNRRLKENEPSFTVTSHCLDEMVHPENDRGLTPRETARLQSFPDWYVFEGDY VKFHSDPLQDKYEQCGDAIPVLLVRALAKQLKMALDEIDFCDKK
Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]
COG id: COG0270
COG function: function code L; Site-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the C5-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018117 - InterPro: IPR001525 [H]
Pfam domain/function: PF00145 DNA_methylase [H]
EC number: =2.1.1.37 [H]
Molecular weight: Translated: 51090; Mature: 51090
Theoretical pI: Translated: 6.46; Mature: 6.46
Prosite motif: PS00094 C5_MTASE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYTYIDLFAGPGGLCTGFKNAGFKPLIAVEMSDNTVKTYARNHDAEIYKLEELIYNKGKL CCEEEEEECCCCCHHCCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCCH ENILNIENNRTALIHGDIRLVDNDIIKEILHKKFKVDSVDVVAGGPPCESFSMAGKRADG HHHHCCCCCCEEEEECCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHCCCCCCCC DERDNLFENMLRIAQATKSKYIFFENVPGLLTKKSNNINVFNVIADEFNNHGYKLASIDK CHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCEEEEEEHHHHCCCCEEEEHHHH NIIKCLASDYGVPQNRERVFLVAINEKYGDNKFIYPEKTHGEGRKYPYITVGEAFKYLPS HHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCCCCCEEEHHHHHHHCCC LNSGGGEEIQQIHYYYEEEYKNNKISEGFYEYIKFISGKEGYIPEHINHDECVNEIANHK CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH AVNHREKMIKRLSYIKQGEGMKKAAERLINEGKEDIVEMYFPKKLYAARNRRLKENEPSF HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCCCCCCCE TVTSHCLDEMVHPENDRGLTPRETARLQSFPDWYVFEGDYVKFHSDPLQDKYEQCGDAIP EHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEECCEEEECCCCHHHHHHHHCCHHH VLLVRALAKQLKMALDEIDFCDKK HHHHHHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure MYTYIDLFAGPGGLCTGFKNAGFKPLIAVEMSDNTVKTYARNHDAEIYKLEELIYNKGKL CCEEEEEECCCCCHHCCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCCH ENILNIENNRTALIHGDIRLVDNDIIKEILHKKFKVDSVDVVAGGPPCESFSMAGKRADG HHHHCCCCCCEEEEECCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHCCCCCCCC DERDNLFENMLRIAQATKSKYIFFENVPGLLTKKSNNINVFNVIADEFNNHGYKLASIDK CHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCEEEEEEHHHHCCCCEEEEHHHH NIIKCLASDYGVPQNRERVFLVAINEKYGDNKFIYPEKTHGEGRKYPYITVGEAFKYLPS HHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCCCCCEEEHHHHHHHCCC LNSGGGEEIQQIHYYYEEEYKNNKISEGFYEYIKFISGKEGYIPEHINHDECVNEIANHK CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH AVNHREKMIKRLSYIKQGEGMKKAAERLINEGKEDIVEMYFPKKLYAARNRRLKENEPSF HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCCCCCCCE TVTSHCLDEMVHPENDRGLTPRETARLQSFPDWYVFEGDYVKFHSDPLQDKYEQCGDAIP EHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEECCEEEECCCCHHHHHHHHCCHHH VLLVRALAKQLKMALDEIDFCDKK HHHHHHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2823226 [H]