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The map label for this gene is pheT

Identifier: 170756823

GI number: 170756823

Start: 3390122

End: 3392503

Strand: Reverse

Name: pheT

Synonym: CLD_1411

Alternate gene names: 170756823

Gene position: 3392503-3390122 (Counterclockwise)

Preceding gene: 170755238

Following gene: 170757781

Centisome position: 85.71

GC content: 30.18

Gene sequence:

>2382_bases
ATGAAAGTTCCAGTTAAATGGTTAAATGATTATATAGATATAGATATTTCTCCAAAGGAATTAGGGGATAGACTTACGTT
ATCAGGTTCAAAGGTAGAAGAAGTTGTTACTACAGGTGATGAAATACAAAATGTAGTTACAGGAAAAATAGTGGAGATAA
GAAAACATCCTGATGCAGATAAACTTTCCATTTGTCAAGTAGATATAGGCAAAGAAGAAAATATTCAAATAGTTACAGCT
GCTACAAATATGAAAGAAATGGATATAATACCTGTAGCTCTTCACGGATCCACATTACATGGTGGATTAAAAATAAAAAA
AGGTAAATTAAGAGGAGAAGTATCTAATGGTATGTTCTGTTCTGAGGAAGAGCTAGGTCTAGCAGGAGATGAACCTGTAT
ATGGACTTATGATTTTGGATCAAGATACACCTATAGGAGAAGATATAAAAAAGGTATTAAAAATGGAAAGCTCTATTATA
GAGTTTGAAATTACATCTAATAGACCAGATTGTTTAAGTGTAATAGGTATAGCAAGGGAAACTGCTGCTACTTTAAACAA
AAAATATACTATTCCGGTGGTAGAATTTTCTCCAAAATGCAGTGAAAGTATAAAGGACTCTTTAAAAGTAGAAGTAAAAG
ATGCACTTTGTAGAAGATATATGGCAAGAGGTATTAAAAATGTTAAAGTAGAACAATCACCAGGCTGGATGCAAGAAAGA
TTATTAGAGGCAGGAGTTAGACCTATAAACAATATAGTAGATATAACAAACTTTGTAATGTTAGAATTAGGTCAACCAAT
GCATGCTTTTGATAGGAAAGAAATAAAAACCAATACTATAGTGGTGGAAAGAGCAAAAGAGAGAGAAAAATTTACTACAT
TAGATGAAACAGAAAGAGAATTAGATGTAGATATACTGAACATAAAAGATGGAGATAGGACTGTAGCTATTGCTGGTATA
ATGGGTGGTTTAAACTCAGAAGTAAAAGAAGATACAAAGGAGATAATATTAGAATGTGCTAATTTTGATGGGACTAATAT
AAGAATATCTTCTAAAAAATTGGGTTTAAGAACAGAAGCCTCTTCTAGGTTTGAAAAAGATTTAGATCCTAATTTAGTGG
AATTAGCTATGGATAGAGCTTGTAAATTAATTCAAGAACTAGGTGCAGGAGAGGTAATGGAAGGTTCTGTAGATGTATAT
GAAAATAAATTAGAGCCTCATATGCTAGAAGTAGACAGCAATTGGATAAATAAATTTTTAGGTACCAATATATCCAAAGA
AGAAATGCAAGAATTTTTAGATAGATTAGATCTTAACACAGAAATAAAGGGAGAAATTTTAGAAATACATGTGCCAACTT
TTAGATGCGATTTAAATATAAAGGAAGATATAGCAGAAGAAATAGCAAGAATATATGGATACAATAATATTCCGATAACT
ACAATAAAAAGTGTTACTATGCAAGCAGGAAAAAATGAAAAGCAACATTTAGATGACAAGGTTATAGAAACACTAATGGC
TAGTGGATTAAATCAATCTATAAGTTATTCCTTCGTAAGTCCAAAAGTATTTGAAAAGATTAATTTAAAAGAAGATAGTA
ATTTAAGAAAAGTAGTTAAGATAAAAAATCCTCTAGGAGAAGATTTTAGTATAATGAGAACTACTACAATACCTTCTATG
GTGGAGGCTTTAAGTAGAAATTACTCAAGAAACAATGATTATGCTTCTCTGTTTGAAATCGGAAAGATATATATTCCAAA
GGAAGATCCTACAGAACTTCCAGATGAAAAGAATATTCTTACTTTAGGTTTATATGGTGATGTGGATTACTTTAATTTAA
AAGGTATAGTAGAAAATATACTAGATGGATTAGGAATAGATAATTATAAATTAAAAAGACAAGAGGAAAATCCTACTTTT
CATCCTGGAAGAACAGCAGAACTATATATAAAGAAAGATTATGTAGGGATATTAGGAGAAATACATCCAGATGTACAAGA
TAATTATGATATAGAAGAAAGATGCTATGTAGCAGAACTAAATTTAGATGTTTTATATAAACATTCTAATGTTAAAAAGG
AGTATAGTCAGTTACCAAAATTCCCAGCAGTAACTAGAGACATAGCAGTAATTGTGGATGATACAGTGCTAGTACAAGAA
ATAGAAAATATAATTAAGAATGGTGGAGGAAACATACTTGAAAGTATTCACCTATTTGATGTCTATAAAGGAAAGCAAAT
ACCAGAAGGAAAGAAGAGTATAGCATATTCTATAGTGTATAGAAATGCTCAAAGAACCCTTAAAGATTCAGAAGTAAATA
AAGTCCATGATAAAATAGTAAGAACTTTAGAACATAAATTAGGAGCACAACTTAGAGATTAA

Upstream 100 bases:

>100_bases
TAGACATACACTTCTAGTTTAGTTGTATAAAATAGAAGTTTAAATAAATATTTTGAAAATAAAATATTTGCTAATTATAT
TATATGAGGAGGTATAACAA

Downstream 100 bases:

>100_bases
AACTAAAATCCTGTTTTATAAATGTATATTTTATGATAGAATTATTATAGGAGATTATGACAAATAGTATATATTTAGAT
AAGGTTTAATCATTGAAACA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MKVPVKWLNDYIDIDISPKELGDRLTLSGSKVEEVVTTGDEIQNVVTGKIVEIRKHPDADKLSICQVDIGKEENIQIVTA
ATNMKEMDIIPVALHGSTLHGGLKIKKGKLRGEVSNGMFCSEEELGLAGDEPVYGLMILDQDTPIGEDIKKVLKMESSII
EFEITSNRPDCLSVIGIARETAATLNKKYTIPVVEFSPKCSESIKDSLKVEVKDALCRRYMARGIKNVKVEQSPGWMQER
LLEAGVRPINNIVDITNFVMLELGQPMHAFDRKEIKTNTIVVERAKEREKFTTLDETERELDVDILNIKDGDRTVAIAGI
MGGLNSEVKEDTKEIILECANFDGTNIRISSKKLGLRTEASSRFEKDLDPNLVELAMDRACKLIQELGAGEVMEGSVDVY
ENKLEPHMLEVDSNWINKFLGTNISKEEMQEFLDRLDLNTEIKGEILEIHVPTFRCDLNIKEDIAEEIARIYGYNNIPIT
TIKSVTMQAGKNEKQHLDDKVIETLMASGLNQSISYSFVSPKVFEKINLKEDSNLRKVVKIKNPLGEDFSIMRTTTIPSM
VEALSRNYSRNNDYASLFEIGKIYIPKEDPTELPDEKNILTLGLYGDVDYFNLKGIVENILDGLGIDNYKLKRQEENPTF
HPGRTAELYIKKDYVGILGEIHPDVQDNYDIEERCYVAELNLDVLYKHSNVKKEYSQLPKFPAVTRDIAVIVDDTVLVQE
IENIIKNGGGNILESIHLFDVYKGKQIPEGKKSIAYSIVYRNAQRTLKDSEVNKVHDKIVRTLEHKLGAQLRD

Sequences:

>Translated_793_residues
MKVPVKWLNDYIDIDISPKELGDRLTLSGSKVEEVVTTGDEIQNVVTGKIVEIRKHPDADKLSICQVDIGKEENIQIVTA
ATNMKEMDIIPVALHGSTLHGGLKIKKGKLRGEVSNGMFCSEEELGLAGDEPVYGLMILDQDTPIGEDIKKVLKMESSII
EFEITSNRPDCLSVIGIARETAATLNKKYTIPVVEFSPKCSESIKDSLKVEVKDALCRRYMARGIKNVKVEQSPGWMQER
LLEAGVRPINNIVDITNFVMLELGQPMHAFDRKEIKTNTIVVERAKEREKFTTLDETERELDVDILNIKDGDRTVAIAGI
MGGLNSEVKEDTKEIILECANFDGTNIRISSKKLGLRTEASSRFEKDLDPNLVELAMDRACKLIQELGAGEVMEGSVDVY
ENKLEPHMLEVDSNWINKFLGTNISKEEMQEFLDRLDLNTEIKGEILEIHVPTFRCDLNIKEDIAEEIARIYGYNNIPIT
TIKSVTMQAGKNEKQHLDDKVIETLMASGLNQSISYSFVSPKVFEKINLKEDSNLRKVVKIKNPLGEDFSIMRTTTIPSM
VEALSRNYSRNNDYASLFEIGKIYIPKEDPTELPDEKNILTLGLYGDVDYFNLKGIVENILDGLGIDNYKLKRQEENPTF
HPGRTAELYIKKDYVGILGEIHPDVQDNYDIEERCYVAELNLDVLYKHSNVKKEYSQLPKFPAVTRDIAVIVDDTVLVQE
IENIIKNGGGNILESIHLFDVYKGKQIPEGKKSIAYSIVYRNAQRTLKDSEVNKVHDKIVRTLEHKLGAQLRD
>Mature_793_residues
MKVPVKWLNDYIDIDISPKELGDRLTLSGSKVEEVVTTGDEIQNVVTGKIVEIRKHPDADKLSICQVDIGKEENIQIVTA
ATNMKEMDIIPVALHGSTLHGGLKIKKGKLRGEVSNGMFCSEEELGLAGDEPVYGLMILDQDTPIGEDIKKVLKMESSII
EFEITSNRPDCLSVIGIARETAATLNKKYTIPVVEFSPKCSESIKDSLKVEVKDALCRRYMARGIKNVKVEQSPGWMQER
LLEAGVRPINNIVDITNFVMLELGQPMHAFDRKEIKTNTIVVERAKEREKFTTLDETERELDVDILNIKDGDRTVAIAGI
MGGLNSEVKEDTKEIILECANFDGTNIRISSKKLGLRTEASSRFEKDLDPNLVELAMDRACKLIQELGAGEVMEGSVDVY
ENKLEPHMLEVDSNWINKFLGTNISKEEMQEFLDRLDLNTEIKGEILEIHVPTFRCDLNIKEDIAEEIARIYGYNNIPIT
TIKSVTMQAGKNEKQHLDDKVIETLMASGLNQSISYSFVSPKVFEKINLKEDSNLRKVVKIKNPLGEDFSIMRTTTIPSM
VEALSRNYSRNNDYASLFEIGKIYIPKEDPTELPDEKNILTLGLYGDVDYFNLKGIVENILDGLGIDNYKLKRQEENPTF
HPGRTAELYIKKDYVGILGEIHPDVQDNYDIEERCYVAELNLDVLYKHSNVKKEYSQLPKFPAVTRDIAVIVDDTVLVQE
IENIIKNGGGNILESIHLFDVYKGKQIPEGKKSIAYSIVYRNAQRTLKDSEVNKVHDKIVRTLEHKLGAQLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=381, Percent_Identity=25.4593175853018, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI1788006, Length=814, Percent_Identity=35.1351351351351, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17533403, Length=285, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355211, Length=388, Percent_Identity=22.680412371134, Blast_Score=96, Evalue=1e-19,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR002319
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01409 tRNA-synt_2d; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 89514; Mature: 89514

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVPVKWLNDYIDIDISPKELGDRLTLSGSKVEEVVTTGDEIQNVVTGKIVEIRKHPDAD
CCCCHHHHCCEEEECCCHHHHCCEEEECCCHHHHHHHCCHHHHHHHHCEEEEEECCCCCC
KLSICQVDIGKEENIQIVTAATNMKEMDIIPVALHGSTLHGGLKIKKGKLRGEVSNGMFC
CEEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEECCEEECCEEEECCCCCEECCCCEEE
SEEELGLAGDEPVYGLMILDQDTPIGEDIKKVLKMESSIIEFEITSNRPDCLSVIGIARE
CCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
TAATLNKKYTIPVVEFSPKCSESIKDSLKVEVKDALCRRYMARGIKNVKVEQSPGWMQER
HHHHCCCCEEEEEEEECCCHHHHHHHHHEEHHHHHHHHHHHHCCCCCCEEECCCCHHHHH
LLEAGVRPINNIVDITNFVMLELGQPMHAFDRKEIKTNTIVVERAKEREKFTTLDETERE
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHCCHH
LDVDILNIKDGDRTVAIAGIMGGLNSEVKEDTKEIILECANFDGTNIRISSKKLGLRTEA
CCEEEEEECCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCH
SSRFEKDLDPNLVELAMDRACKLIQELGAGEVMEGSVDVYENKLEPHMLEVDSNWINKFL
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHCCCCCEEEEECHHHHHHHH
GTNISKEEMQEFLDRLDLNTEIKGEILEIHVPTFRCDLNIKEDIAEEIARIYGYNNIPIT
CCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECEEEECCCCHHHHHHHHHHHHCCCCCCCH
TIKSVTMQAGKNEKQHLDDKVIETLMASGLNQSISYSFVSPKVFEKINLKEDSNLRKVVK
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHE
IKNPLGEDFSIMRTTTIPSMVEALSRNYSRNNDYASLFEIGKIYIPKEDPTELPDEKNIL
ECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCCCCCCCCCCEE
TLGLYGDVDYFNLKGIVENILDGLGIDNYKLKRQEENPTFHPGRTAELYIKKDYVGILGE
EEEEECCCCEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHC
IHPDVQDNYDIEERCYVAELNLDVLYKHSNVKKEYSQLPKFPAVTRDIAVIVDDTVLVQE
CCCCCCCCCCHHHCEEEEEECEEEEEECCCHHHHHHHCCCCCCCCCCEEEEECCHHHHHH
IENIIKNGGGNILESIHLFDVYKGKQIPEGKKSIAYSIVYRNAQRTLKDSEVNKVHDKIV
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHH
RTLEHKLGAQLRD
HHHHHHHCCCCCC
>Mature Secondary Structure
MKVPVKWLNDYIDIDISPKELGDRLTLSGSKVEEVVTTGDEIQNVVTGKIVEIRKHPDAD
CCCCHHHHCCEEEECCCHHHHCCEEEECCCHHHHHHHCCHHHHHHHHCEEEEEECCCCCC
KLSICQVDIGKEENIQIVTAATNMKEMDIIPVALHGSTLHGGLKIKKGKLRGEVSNGMFC
CEEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEECCEEECCEEEECCCCCEECCCCEEE
SEEELGLAGDEPVYGLMILDQDTPIGEDIKKVLKMESSIIEFEITSNRPDCLSVIGIARE
CCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
TAATLNKKYTIPVVEFSPKCSESIKDSLKVEVKDALCRRYMARGIKNVKVEQSPGWMQER
HHHHCCCCEEEEEEEECCCHHHHHHHHHEEHHHHHHHHHHHHCCCCCCEEECCCCHHHHH
LLEAGVRPINNIVDITNFVMLELGQPMHAFDRKEIKTNTIVVERAKEREKFTTLDETERE
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHCCHH
LDVDILNIKDGDRTVAIAGIMGGLNSEVKEDTKEIILECANFDGTNIRISSKKLGLRTEA
CCEEEEEECCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCH
SSRFEKDLDPNLVELAMDRACKLIQELGAGEVMEGSVDVYENKLEPHMLEVDSNWINKFL
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHCCCCCEEEEECHHHHHHHH
GTNISKEEMQEFLDRLDLNTEIKGEILEIHVPTFRCDLNIKEDIAEEIARIYGYNNIPIT
CCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECEEEECCCCHHHHHHHHHHHHCCCCCCCH
TIKSVTMQAGKNEKQHLDDKVIETLMASGLNQSISYSFVSPKVFEKINLKEDSNLRKVVK
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHE
IKNPLGEDFSIMRTTTIPSMVEALSRNYSRNNDYASLFEIGKIYIPKEDPTELPDEKNIL
ECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCCCCCCCCCCEE
TLGLYGDVDYFNLKGIVENILDGLGIDNYKLKRQEENPTFHPGRTAELYIKKDYVGILGE
EEEEECCCCEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHC
IHPDVQDNYDIEERCYVAELNLDVLYKHSNVKKEYSQLPKFPAVTRDIAVIVDDTVLVQE
CCCCCCCCCCHHHCEEEEEECEEEEEECCCHHHHHHHCCCCCCCCCCEEEEECCHHHHHH
IENIIKNGGGNILESIHLFDVYKGKQIPEGKKSIAYSIVYRNAQRTLKDSEVNKVHDKIV
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHH
RTLEHKLGAQLRD
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]