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The map label for this gene is ynjE [H]

Identifier: 170756660

GI number: 170756660

Start: 1269119

End: 1270501

Strand: Direct

Name: ynjE [H]

Synonym: CLD_3422

Alternate gene names: 170756660

Gene position: 1269119-1270501 (Clockwise)

Preceding gene: 170757251

Following gene: 170756018

Centisome position: 32.06

GC content: 30.44

Gene sequence:

>1383_bases
GTGAAAAAAAATTTTAAAGGAATAGTGCTATTGCTATTCGGTATTATGATGTTTACATTAATAGGTTGTACTAGTAATGA
AAAAACAGAGAAGAAGGAAGAGAGTACTTCTTTAGTAAAGGAAATTAAAACAGATGAATTAAAGAAGAATATAACATCCA
AGGAATGGGTAGTATTAGATACTAGATCTAATGATGCTTTTAATGGCTGGAAATTAGATGGAGTAAAAAGAGGTGGACAT
ATAAAAGGTGCTACTGATTTTTCAGCAAATTGGCTAAAAGTAGAAGATACTGAAAAGAATAAAGAAAAAAGATTAGAAGA
GGCTTTAAAAAATAAAGGTATCACAAAGGATAAAAATGTAGTTTTATATGATGCTAATGGAAAAGATGCAGAAGAAGTAG
CAAAATATTTAAATAAAAAAGGAATAACTAAATTATATAAATATGATGTTAAAGAATGGGCAGAAGATGATAAACTTGCA
ATGGAAAGTTATCCTAATTATCAAATGTTAGTACCAGCTAGTTGGGTTAATGATTTAATAAATAATAAAAAACCAGAAAC
TTTTAAAGGAGATAAATATAAGGTTTTTGAAGTGAGCTGGGGAGATGAATCTAAAGCAGAAGATTACAAAAAAGGACATA
TTAAAGGTGCTGTACATATAAATACAGATGAAGTTGAAAAAGGACCAGTATGGAATCGTCTACCAGATAAGGATTTAGAA
AAGTTTGCTAAAAATAATGGTATTACAGCAGATACAACAGTAGTGTTATATGGAGCAGATTCTATGCCTTCATTTAGAGT
AGCAGCAATATTAAAATATATGGGTGTTAAAGATGTAAGAGTTTTAAATGGTGGTACAGCTGCTTGGACTAGTGCTGGAT
ATGAGCTAGATACAACATCAAATAAGAAAACTCCAGTAGATTCTTTTGGAGTAGCGGTACCTCTAAATAAAGGCTATATA
GTAGATTTACCTGAAGCTAAAGAAATATTAAAAGATAAAGAAGGAAGTAAACTTGTTGATATAAGAAGTTGGGATGAATT
TATTGGAAAAACTTCAGGATATGATTATATAAAAGCTAAAGGTCGTCCAGTAGGAGCAGTATGGGGACATGCCGGATCAG
ATAATTCACATTTAGAAGATTTTAGAAATGTAGATAATACTATGAGAAATTCATCAGAAATATTATCTATGTGGGAAAAA
GAAGGTATAAAACCAGATCAAAGATTATCTTTTTATTGTGGAACTGGTTGGAGAGCAGCTGAAGTTTTAATTTACGCTGA
TGTTATGGGATTAAAAAATATATCTTTATATGACGGTGGATGGAATGAATGGAGTTTAGATAAAAACAATCCAATAGAGG
TTGGAGACCCTTCTAAAAAATAG

Upstream 100 bases:

>100_bases
CTATAATTATTTTTTATTTTAAAATATAAATTAAATATCGTAAAATGTAAAATATAATAAATACAAAATTGAAAATTATA
CATGAAAAAGGGGTGTAAAA

Downstream 100 bases:

>100_bases
TAAATATGTAGTTACAATAATTTTAATAAATAAAAGAGTGTGTCTTAAAATAAAATAGATTTAATTTTTATGTAATATAT
TCTATAGAGCAATTTATTTT

Product: putative thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKKNFKGIVLLLFGIMMFTLIGCTSNEKTEKKEESTSLVKEIKTDELKKNITSKEWVVLDTRSNDAFNGWKLDGVKRGGH
IKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNVVLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLA
MESYPNYQMLVPASWVNDLINNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTTSNKKTPVDSFGVAVPLNKGYI
VDLPEAKEILKDKEGSKLVDIRSWDEFIGKTSGYDYIKAKGRPVGAVWGHAGSDNSHLEDFRNVDNTMRNSSEILSMWEK
EGIKPDQRLSFYCGTGWRAAEVLIYADVMGLKNISLYDGGWNEWSLDKNNPIEVGDPSKK

Sequences:

>Translated_460_residues
MKKNFKGIVLLLFGIMMFTLIGCTSNEKTEKKEESTSLVKEIKTDELKKNITSKEWVVLDTRSNDAFNGWKLDGVKRGGH
IKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNVVLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLA
MESYPNYQMLVPASWVNDLINNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTTSNKKTPVDSFGVAVPLNKGYI
VDLPEAKEILKDKEGSKLVDIRSWDEFIGKTSGYDYIKAKGRPVGAVWGHAGSDNSHLEDFRNVDNTMRNSSEILSMWEK
EGIKPDQRLSFYCGTGWRAAEVLIYADVMGLKNISLYDGGWNEWSLDKNNPIEVGDPSKK
>Mature_460_residues
MKKNFKGIVLLLFGIMMFTLIGCTSNEKTEKKEESTSLVKEIKTDELKKNITSKEWVVLDTRSNDAFNGWKLDGVKRGGH
IKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNVVLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLA
MESYPNYQMLVPASWVNDLINNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTTSNKKTPVDSFGVAVPLNKGYI
VDLPEAKEILKDKEGSKLVDIRSWDEFIGKTSGYDYIKAKGRPVGAVWGHAGSDNSHLEDFRNVDNTMRNSSEILSMWEK
EGIKPDQRLSFYCGTGWRAAEVLIYADVMGLKNISLYDGGWNEWSLDKNNPIEVGDPSKK

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=288, Percent_Identity=26.0416666666667, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI194473668, Length=302, Percent_Identity=24.8344370860927, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI61835204, Length=301, Percent_Identity=24.5847176079734, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI194473681, Length=301, Percent_Identity=24.5847176079734, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI87081967, Length=446, Percent_Identity=39.237668161435, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI87082121, Length=266, Percent_Identity=24.812030075188, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 51814; Mature: 51814

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNFKGIVLLLFGIMMFTLIGCTSNEKTEKKEESTSLVKEIKTDELKKNITSKEWVVLD
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
TRSNDAFNGWKLDGVKRGGHIKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCE
VLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLAMESYPNYQMLVPASWVNDLI
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECHHHHHHHH
NNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTTS
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCEECCCCCEEEECCC
NKKTPVDSFGVAVPLNKGYIVDLPEAKEILKDKEGSKLVDIRSWDEFIGKTSGYDYIKAK
CCCCCHHHCCEEEECCCCEEEECCHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCEEEEC
GRPVGAVWGHAGSDNSHLEDFRNVDNTMRNSSEILSMWEKEGIKPDQRLSFYCGTGWRAA
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEECCCCCHH
EVLIYADVMGLKNISLYDGGWNEWSLDKNNPIEVGDPSKK
EEEEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKNFKGIVLLLFGIMMFTLIGCTSNEKTEKKEESTSLVKEIKTDELKKNITSKEWVVLD
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
TRSNDAFNGWKLDGVKRGGHIKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCE
VLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLAMESYPNYQMLVPASWVNDLI
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECHHHHHHHH
NNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTTS
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCEECCCCCEEEECCC
NKKTPVDSFGVAVPLNKGYIVDLPEAKEILKDKEGSKLVDIRSWDEFIGKTSGYDYIKAK
CCCCCHHHCCEEEECCCCEEEECCHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCEEEEC
GRPVGAVWGHAGSDNSHLEDFRNVDNTMRNSSEILSMWEKEGIKPDQRLSFYCGTGWRAA
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEECCCCCHH
EVLIYADVMGLKNISLYDGGWNEWSLDKNNPIEVGDPSKK
EEEEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9097039 [H]