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The map label for this gene is aspS

Identifier: 170756537

GI number: 170756537

Start: 3297384

End: 3299165

Strand: Reverse

Name: aspS

Synonym: CLD_1486

Alternate gene names: 170756537

Gene position: 3299165-3297384 (Counterclockwise)

Preceding gene: 170754518

Following gene: 170756815

Centisome position: 83.35

GC content: 30.53

Gene sequence:

>1782_bases
ATGGGAGAAGCGTTAAGAGGACTTAAGAGAACAATAATGTGTGGCGAGTCTAGAGAAAATAATATAGGTGAAAAGGTCAC
AGTTATGGGATGGGTTCAAAGAAAAAGAAATTTAGGAGGACTAATCTTTGTAGATTTAAGAGATAGAACAGGAATTATGC
AAATAGTTTTTGGTGAAGAAATAAATAAAGAAGCCTTCGAAAAATCAGATAATGTAAAATCAGAATATTGTATCGCTGTA
ACTGGTGAAATAGTTAAAAGACAGTCACCAAACAATGATATGGAAACTGGAGCTGTGGAATTAAAGGGAGAAGATATAAA
AATATTATCTGAATCAGAAACACCTCCTATATATATAAAAGAAGGGTTAGATGCATCTGAAAATATAAGATTAAAATATA
GATATCTTGACTTAAGAAGACCAGATATGCAAAAGATATTTATGATAAGACACAAGACATGTAAAGTTGTTAGAGATTTC
TTAGATGAAAATGGATTTTTAGAAATGGAAACACCAATACTTACAAAAAGTACCCCAGAAGGAGCTCGTGACTATTTAGT
TCCAAGTAGAAATTACAAAGGAATGTTTTATGCATTACCACAATCACCACAAATATTTAAACAGCTTTTAATGGTCTCAG
GTTATGATAAATATTTTCAAATAACAAAATGTTTTAGAGATGAAGATTTAAGAGCTAATAGACAACCGGAATTTACACAA
ATAGATATGGAATTATCTTTTGTAGAAGAAGATGATGTAATTGAATTAAACGAAAGATTATTAGCTAAGGTATTTAAAGA
AGTTGGGGGAATAGATGTAAAACTTCCGATAGAAAGAATGCCATATAAAATAGCTATGGAAAAATACGGTTCAGATAAAC
CAGATTTAAGATTTGGAATGGAAATAAATGATTTAACAGAAGCTGTTAAAAATTCAGAATTTAAAGTATTCAAAGGTGCT
ATAGAAGCAGGTGGCTCTGTAAGAGCAATAAAAGCAGAAAATTGTGCTACTATGGGAAGAAAACAAATAGATAAGCTACA
AGATTTTGTTAAAACATATAAAGCAAAAGGATTAGCTTGGATAGCTTATAAAGAGGATGAAATAAAATCTCCAATAGCTA
AATTTTTAACAGAAGAAGAAATGAAAGCTATATTAGAAAAAATGGATGCTAAAGCAGGAGATTTAATATTAATAGTAGCA
GACAAAAACAATGTAGTATTCGAAAGCTTAGGTGCATTAAGACTACATATAGCAAAAGAATTAGATATTATAAATAAAAA
TGAGTTTAGATTTGTGTGGATAACTGAATTCCCACTATTAGCTTATAATGAAGAAGAAGGCAGATATCAAGCGGAGCATC
ATCCATTCACAGCTATAATGGATGAGGATATAGAACTATTAGATACAGAGCCAGGAAAAGTAAGAGCGAAAGCTTATGAT
ATAGTATTAAATGGAGAAGAGCTAGGCGGAGGAAGTATTAGAATACATGATAGTAAACTTCAAGAAAAAATGTTTAGTGT
ATTAGGCTTTACTAAAGAAAAAGCTTGGGAAAGATTTGGGTTCTTACTTGAAGCATTTAAATTTGGACCACCACCACATG
GTGGGTTAGCTTATGGTCTTGATAGAATGATAATGTTTTTAGCAGGAACAGAGAATATTAAAGATGTAATAACATTCCCT
AAGAATCAAAATGCATTCTGTCCATTAACAGAAGCACCAAATGTAGTTGATGAAAATCAATTAGAAGAATTAGGAATAAA
AAAAATAGAAAAAGAAGATTAA

Upstream 100 bases:

>100_bases
GGGAAGCAAATAGAAGTCGATATAAAAGAGATAGATACATTAATTAAAGTATTTAAATAGACAAAAATAAAATAACAAAT
CTATATTTGGAGGAATAAAA

Downstream 100 bases:

>100_bases
TAAAATAAGGAAAGTGCGATGCGCTTTCCTTATTTTATCTATTAAGTATTCGTGTCATTTTAAGAGTGTAACTTTGGTTT
TTACCTAATTTTTTATCCAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MGEALRGLKRTIMCGESRENNIGEKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEEINKEAFEKSDNVKSEYCIAV
TGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIKEGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDF
LDENGFLEMETPILTKSTPEGARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGMEINDLTEAVKNSEFKVFKGA
IEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAWIAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVA
DKNNVVFESLGALRLHIAKELDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLDTEPGKVRAKAYD
IVLNGEELGGGSIRIHDSKLQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGLDRMIMFLAGTENIKDVITFP
KNQNAFCPLTEAPNVVDENQLEELGIKKIEKED

Sequences:

>Translated_593_residues
MGEALRGLKRTIMCGESRENNIGEKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEEINKEAFEKSDNVKSEYCIAV
TGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIKEGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDF
LDENGFLEMETPILTKSTPEGARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGMEINDLTEAVKNSEFKVFKGA
IEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAWIAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVA
DKNNVVFESLGALRLHIAKELDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLDTEPGKVRAKAYD
IVLNGEELGGGSIRIHDSKLQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGLDRMIMFLAGTENIKDVITFP
KNQNAFCPLTEAPNVVDENQLEELGIKKIEKED
>Mature_592_residues
GEALRGLKRTIMCGESRENNIGEKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEEINKEAFEKSDNVKSEYCIAVT
GEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIKEGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDFL
DENGFLEMETPILTKSTPEGARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQI
DMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGMEINDLTEAVKNSEFKVFKGAI
EAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAWIAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVAD
KNNVVFESLGALRLHIAKELDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLDTEPGKVRAKAYDI
VLNGEELGGGSIRIHDSKLQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGLDRMIMFLAGTENIKDVITFPK
NQNAFCPLTEAPNVVDENQLEELGIKKIEKED

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=603, Percent_Identity=37.6451077943615, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI194272210, Length=251, Percent_Identity=29.0836653386454, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI5031815, Length=251, Percent_Identity=29.0836653386454, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI45439306, Length=285, Percent_Identity=25.9649122807018, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI4758762, Length=282, Percent_Identity=26.9503546099291, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI226491502, Length=261, Percent_Identity=25.2873563218391, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=47.2081218274112, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=26.8041237113402, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789256, Length=281, Percent_Identity=25.2669039145907, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=595, Percent_Identity=34.7899159663866, Blast_Score=314, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI71994340, Length=302, Percent_Identity=25.8278145695364, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=302, Percent_Identity=25.8278145695364, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=302, Percent_Identity=25.8278145695364, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=270, Percent_Identity=28.1481481481481, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71984122, Length=295, Percent_Identity=25.0847457627119, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=626, Percent_Identity=35.7827476038339, Blast_Score=300, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6321807, Length=254, Percent_Identity=28.740157480315, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=285, Percent_Identity=27.719298245614, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=255, Percent_Identity=25.4901960784314, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=271, Percent_Identity=22.5092250922509, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=35.61872909699, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=30.5439330543933, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24640849, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640851, Length=289, Percent_Identity=26.9896193771626, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67834; Mature: 67703

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEALRGLKRTIMCGESRENNIGEKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEE
CCHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECCC
INKEAFEKSDNVKSEYCIAVTGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIK
CCHHHHHCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCEEEE
EGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDFLDENGFLEMETPILTKSTPE
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCC
GARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
CCHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEE
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGM
EEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECHHHCCCEEEHHHCCCCCCCEEECC
EINDLTEAVKNSEFKVFKGAIEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAW
CHHHHHHHHHCCCEEEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVADKNNVVFESLGALRLHIAKE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHCHHHHHHHHH
LDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLDTEPGKVRAKAYD
HCEECCCCEEEEEEECCCEEEEECCCCCEECCCCCEEEEECCCCCEECCCCCEEEEEEEE
IVLNGEELGGGSIRIHDSKLQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGL
EEEECCCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DRMIMFLAGTENIKDVITFPKNQNAFCPLTEAPNVVDENQLEELGIKKIEKED
HHHHHHHCCCCCCHHHEECCCCCCCEECCCCCCCCCCCHHHHHHCHHHHCCCC
>Mature Secondary Structure 
GEALRGLKRTIMCGESRENNIGEKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEE
CHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECCC
INKEAFEKSDNVKSEYCIAVTGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIK
CCHHHHHCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCEEEE
EGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDFLDENGFLEMETPILTKSTPE
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCC
GARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
CCHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEE
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGM
EEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECHHHCCCEEEHHHCCCCCCCEEECC
EINDLTEAVKNSEFKVFKGAIEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAW
CHHHHHHHHHCCCEEEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVADKNNVVFESLGALRLHIAKE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHCHHHHHHHHH
LDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLDTEPGKVRAKAYD
HCEECCCCEEEEEEECCCEEEEECCCCCEECCCCCEEEEECCCCCEECCCCCEEEEEEEE
IVLNGEELGGGSIRIHDSKLQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGL
EEEECCCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DRMIMFLAGTENIKDVITFPKNQNAFCPLTEAPNVVDENQLEELGIKKIEKED
HHHHHHHCCCCCCHHHEECCCCCCCEECCCCCCCCCCCHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA