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The map label for this gene is hisS

Identifier: 170754518

GI number: 170754518

Start: 3299206

End: 3300453

Strand: Reverse

Name: hisS

Synonym: CLD_1485

Alternate gene names: 170754518

Gene position: 3300453-3299206 (Counterclockwise)

Preceding gene: 170755567

Following gene: 170756537

Centisome position: 83.38

GC content: 29.41

Gene sequence:

>1248_bases
ATGTCATTGCAAGCGCCAAAAGGTACAAAAGATTTATTGCCTACAGAATCATATAAGTGGCAATATTTAGAGAATAAATT
TAGAAATATAGCAGCAGATTTTGGCTGTAGAGAAATAAGAACTCCAGTTTTTGAATATACAGAATTATTTCAAAGAGGAG
TAGGGGAAACTACAGATGTAGTTCAAAAGGAAATGTATACTTTTGAAGATAAAGCTGGTAGAAGTATAACTTTAAAACCA
GAAGGAACATCACCAGCAGTAAGAGCCTTCGTTGAAGGAAGATTATTCAATGAAACTCAACCTACTAAGATGTATTATTT
TACACCTGTTATGAGATATGAGAATGTTCAAAAGGGAAGACTTAGACAACATCATCAATTTGGAATAGAAATTTTTGGAG
CAAAAGATGCATCTGTGGATGCAGAAGTTATAAGTATACCTGTTGGAATATATAAAGAATTAGGTGTAGAGGGCGTAGAA
TTAAATATAAATAGCATAGGTTGCCCTAAATGTAGAAAAACTTATAATGAAGCACTAAAAAAATATTTATCTAAAAATTA
TGATAAATTATGCTCAACTTGTAAAACTAGATTTGATAAAAACCCACTTAGAATATTAGATTGCAAAGTTGATACTTGTA
AAGAAATAGTAAAGGATGCACCAATTATATTAGATTATATATGTGATGAATGTAAGGACCATTTTGAATCATTAAAAAGT
TATTTAGATGTATTGGATATAAAATATAAAGTAGATCCATTTATAGTTAGAGGATTAGATTATTATAGTAAAACTGTGTT
TGAATTTATAATAGATGATATAACTATATGTGCTGGAGGAAGATATGATTATCTTATAGAAGAAATAGGTGGGCCTTCTA
TGCCAGCAGTAGGTTTTGGTATGGGAATAGAAAGATTACTTTTAACATTGCAAGAAAAAGCAATAGAAATTCCTGAAGAA
GCATATGTAGATTTATATTTAGGAAATATGGGAGATAAAGCTAAATTAGAGGTTTTAAAATTAGCTAAAGAATTAAGGGA
TAGACATATAAAGTGTGAAATAGACCATATGGGCAAAAGTGTTAAAGCACAAATGAAGTATGCAAATAGGATAGGTGCTA
AATATTCTATGGTATTAGGTGAAGAAGAATTAAATACTGGAAAGGTATCCCTTAAAAGAATGGAAGATGGGAAGCAAATA
GAAGTCGATATAAAAGAGATAGATACATTAATTAAAGTATTTAAATAG

Upstream 100 bases:

>100_bases
TAAGGATGTAATAGAATATACAAAAAGAGTGGAAGAAATGGTACATAGAAAGAAAAAAATATTAGATACTTTATATTTAT
AATGGTTAGGAGGAATATAA

Downstream 100 bases:

>100_bases
ACAAAAATAAAATAACAAATCTATATTTGGAGGAATAAAAATGGGAGAAGCGTTAAGAGGACTTAAGAGAACAATAATGT
GTGGCGAGTCTAGAGAAAAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKP
EGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVE
LNINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFESLKS
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFGMGIERLLLTLQEKAIEIPEE
AYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGKQI
EVDIKEIDTLIKVFK

Sequences:

>Translated_415_residues
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKP
EGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVE
LNINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFESLKS
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFGMGIERLLLTLQEKAIEIPEE
AYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGKQI
EVDIKEIDTLIKVFK
>Mature_414_residues
SLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKPE
GTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVEL
NINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFESLKSY
LDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFGMGIERLLLTLQEKAIEIPEEA
YVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGKQIE
VDIKEIDTLIKVFK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=456, Percent_Identity=24.3421052631579, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788861, Length=409, Percent_Identity=42.5427872860636, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=23.0434782608696, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71993686, Length=460, Percent_Identity=23.0434782608696, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325290, Length=465, Percent_Identity=24.0860215053763, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47524; Mature: 47392

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDKAGRSITLKPEGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGR
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCHHHH
LRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVELNINSIGCPKCRKTYNEALK
HHHHHHCCEEEEECCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFESLKS
HHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFG
HHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCHHHH
MGIERLLLTLQEKAIEIPEEAYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKS
HHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHCCHH
VKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGKQIEVDIKEIDTLIKVFK
HHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
SLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDKAGRSITLKPEGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGR
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCHHHH
LRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVELNINSIGCPKCRKTYNEALK
HHHHHHCCEEEEECCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFESLKS
HHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFG
HHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCHHHH
MGIERLLLTLQEKAIEIPEEAYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKS
HHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHCCHH
VKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGKQIEVDIKEIDTLIKVFK
HHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA