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The map label for this gene is rnjA [H]

Identifier: 170756499

GI number: 170756499

Start: 1422605

End: 1424269

Strand: Direct

Name: rnjA [H]

Synonym: CLD_3274

Alternate gene names: 170756499

Gene position: 1422605-1424269 (Clockwise)

Preceding gene: 170755356

Following gene: 170757072

Centisome position: 35.94

GC content: 31.95

Gene sequence:

>1665_bases
GTGAGCAATAAAGATAGTGTAAAGGTTATTCCTTTGGGTGGACTTGGAGAAATTGGTAAAAATATAACTGCTTTTGAATA
TGGAAATGAAATAGTAATCATAGATTGTGGTATATCTTTTCCAGATGAGGAGATGTATGGTGTAGATTTGGTTATTCCAG
ATATAACCTACCTTCTAAATAACAAAGATAAAGTAAAAGCTATTTTTCTAACTCATGGACATGAAGATCATATAGGATCT
TTACCTTATATTCTGCAACAATTAAATAGGCCTGTTTATGGAACTGCATTAACCTTAGGTATAGTAGAGAATAAACTAAA
AGAACATAATATGCTTTCAGATTGTGAGCTTAATAAGGTGGAAGCAGGAGATATAGTAGAACTTAAAAATTTAAAGATAG
AATTTATAAGAAATACTCATAGTATAGCAGATTCTTGTTCCATAGCTATTCATACGCCGGTGGGAGTTATATTACATACT
GGCGATTTTAAAATAGACTATACACCTATTGATGGATTAGTAATGGATTTTCATCGTATTGCTGAATTAGGCAAAAAAGG
AGTATTACTTCTTATGGCTGATAGTACAAACGTACAGCGTAAGGGACACACAATTTCCGAGAAATCTATAGGAGAAACTT
TAACTAAAATTTTTTCAAATGCTAAAGGAAGGGTTATTGTAGCAACTTTTGCTTCAAACATACATAGGATGCAGCAAATA
GTAGATGCATCTATAGAGTATGGTAGAAAAGTAGCTTTCAGTGGGAGAAGTATGGAAAATATATCTAAGGTAGCCATGGA
CTTAGGATATCTTCATATACCAGAAACTTATTTAATAACTGTAGATGAAATGAAAAATTATCCGAACGAACAAATAACCA
TAATAACTACTGGAAGTCAAGGAGAACCTATGGCAGCTCTTGCAAGAATAGCTTATTCTAATCATAGAAAAATAGCTATA
GAGTCAAAGGATTTATTTATTATTTCTGCATCACCAATTCCAGGAAATGAAAAACTTATATCTAGAGTAATAAATGAGTT
ATTTAAAAAGGGTGCAGATGTAATATATGAAGCATTAGAAGAGGTACATGTATCTGGTCATGCTTATGAGGAAGAATTAA
AATTAATTCATACATTGGTGCATCCCAAATATTTTATGCCAGTACATGGAGAATACAGGCACCTAAAACGACATGTGGAT
TTAGCCATGGAGTTAGGCATGGAAAGAGAGAACATATTCTCTTTAGAAACAGGGCAAGTTTTAGAAATATCACATGAAGA
AGCAAAAGTATCAGGGAAGGTCCGTACAGGATCTATATTTGTAGATGGTATAGGTGTAGGTGATGTAGGGAATATAGTGC
TCAGAGATAGAAGGTATTTAGCGCAGGACGGTATGTTAACCATAGTAGTAACTCTAGAAAAAGAATCCTACAGCGTAATA
GCGGGACCCGATGTAATAACAAGGGGATTCATATATGTAAAAGAGTCAGAGGATTTAATAAATGAAGTAAAAGGAATAGT
AAAAAAAGAACTAGAAAATTGTTTAGAAAATAAAATAATTGAATGGTATGTATTAAAATCAAATATTAAAAAATCCGTAG
AAAAATATTTATATGAAAAAACAAAGAGAAGGCCAATAGTTTTACCTATAATTATGGAAATTTAA

Upstream 100 bases:

>100_bases
ATAATCATTAGCTCTTTAGTTAATTTTATTAATTTAAAGAGCAGAATAATATAATAGGTATAGCAAAAAATTTATAAATA
TTGAAAGAGGAGGGTATGAA

Downstream 100 bases:

>100_bases
AATTATAAAAAATAACAAAAAGCTAGAACTTTAATGTTAAATTATAGTTCTAGCTTTTTGTTATATATTAACACATTAAA
ATGATGTAAATATAATAAAA

Product: RNA-metabolising metallo-beta-lactamase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGS
LPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHT
GDFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
VDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAI
ESKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVD
LAMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
AGPDVITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI

Sequences:

>Translated_554_residues
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGS
LPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHT
GDFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
VDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAI
ESKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVD
LAMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
AGPDVITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI
>Mature_553_residues
SNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGSL
PYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTG
DFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQIV
DASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAIE
SKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDL
AMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVIA
GPDVITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62097; Mature: 61966

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLN
CCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEEECCCCEECC
NKDKVKAIFLTHGHEDHIGSLPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKV
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHEEEHHHHHHHHHHCCCCCCCCCCCC
EAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTGDFKIDYTPIDGLVMDFHRI
CCCCEEEEECCEEEEECCHHHHCCCCEEEEECCEEEEEECCCEEEEEECCCHHHHHHHHH
AELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
VDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQ
HHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCEEEEEEHHHHCCCCCEEEEEEECCC
GEPMAALARIAYSNHRKIAIESKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALE
CCHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDLAMELGMERENIFSLETGQV
HHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCE
LEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
EEEECCHHHCCCEEEECEEEEEECCCCCCCCEEECCCHHHHCCCCEEEEEEEECCCEEEE
AGPDVITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEK
ECCCHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
TKRRPIVLPIIMEI
HCCCCEEEEEEECC
>Mature Secondary Structure 
SNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLN
CCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEEECCCCEECC
NKDKVKAIFLTHGHEDHIGSLPYILQQLNRPVYGTALTLGIVENKLKEHNMLSDCELNKV
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHEEEHHHHHHHHHHCCCCCCCCCCCC
EAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTGDFKIDYTPIDGLVMDFHRI
CCCCEEEEECCEEEEECCHHHHCCCCEEEEECCEEEEEECCCEEEEEECCCHHHHHHHHH
AELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
VDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQ
HHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCEEEEEEHHHHCCCCCEEEEEEECCC
GEPMAALARIAYSNHRKIAIESKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALE
CCHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDLAMELGMERENIFSLETGQV
HHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCE
LEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
EEEECCHHHCCCEEEECEEEEEECCCCCCCCEEECCCHHHHCCCCEEEEEEEECCCEEEE
AGPDVITRGFIYVKESEDLINEVKGIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEK
ECCCHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
TKRRPIVLPIIMEI
HCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]