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The map label for this gene is pyrB

Identifier: 170756348

GI number: 170756348

Start: 3519281

End: 3520204

Strand: Reverse

Name: pyrB

Synonym: CLD_1290

Alternate gene names: 170756348

Gene position: 3520204-3519281 (Counterclockwise)

Preceding gene: 170755738

Following gene: 170757312

Centisome position: 88.93

GC content: 31.6

Gene sequence:

>924_bases
ATGTTAAAAGGAAGAAATTTATTAGACCCAATGGATTTTTCATTAGAAGAACTAGAAGAGGTATTTAAATTAGCAGATGA
AATAATTGAGGAGCCGGAAAAATTTTTACATGTATGTGATGGAAAAATATTAGCTACATTATTCTACGAGCCAAGTACAA
GAACAAGATTTAGTTTTGAAGCTGCTATGCTTAGACTAGGTGGTCAGGTAATAGGATTTTCAGAGCCTAATTCAAGTTCA
GTAGCAAAAGGAGAAAGTGTGGCAGACACTATAAGAACTGTAGGTTGTTATGCAGATATAGCAGCCATGAGACACCCAAA
AGAAGGTGCACCGGCAATAGCTGCAATGTATTCAGAAATACCAGTTATAAATGCTGGTGATGGTAGTCATCAACATCCAA
CTCAAACATTAACAGATCTATTAACTATAAGATCCTTAAAAGGAGATTTATCTAACTTAACTATAGGTTGCTGTGGAGAT
TTAAAATTTGGAAGAACAGTACACTCATTAGTAAAGGCCCTATCAAGGTATAAGAACAACAAATTTGTTTTTATGTCACC
AGAAGAATTAAAAATTCCTGATTATATAAGAAAAGAAATATTAGAAAAAAACAATATAGAATATAAAGAAGTATCAAAGA
TGGAAGATGCAATGGCAGAACTAGATATACTTTATATGACAAGAGTTCAAAGAGAAAGATTCTTTAATGAAGATGATTAT
GTAAGATTAAAGGATAGCTATATATTAGATGGTGAAAAGATGAGATATGCCAAAAAAGATATGATGGTTCTTCATCCATT
ACCAAGAGTTAATGAAATAGCTTATGAAATAGATCAAGACCCAAGAGGATGTTATTTTAAACAAGCTAAATATGGAATGT
ATGTAAGAATGGCTTTAATAGCAAAATTATTGGGGGTTAGATAA

Upstream 100 bases:

>100_bases
TATTTTAAACTAGAGTATAATTGTGCCTTCCTTTAAAAAAGGAAGGCATTTTTTATACCTTTAAATTTAATCCAGAGAGA
TTAAAAAGGAGGAATTGTAT

Downstream 100 bases:

>100_bases
TATGTTAACAATAAATAGTATAAAAAATGGTATAGTAATAGATCATATACAAGCAGGTCACGGTATAAAAATATTTAAAT
ACTTAGGATTAGAAGAAGCT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLKGRNLLDPMDFSLEELEEVFKLADEIIEEPEKFLHVCDGKILATLFYEPSTRTRFSFEAAMLRLGGQVIGFSEPNSSS
VAKGESVADTIRTVGCYADIAAMRHPKEGAPAIAAMYSEIPVINAGDGSHQHPTQTLTDLLTIRSLKGDLSNLTIGCCGD
LKFGRTVHSLVKALSRYKNNKFVFMSPEELKIPDYIRKEILEKNNIEYKEVSKMEDAMAELDILYMTRVQRERFFNEDDY
VRLKDSYILDGEKMRYAKKDMMVLHPLPRVNEIAYEIDQDPRGCYFKQAKYGMYVRMALIAKLLGVR

Sequences:

>Translated_307_residues
MLKGRNLLDPMDFSLEELEEVFKLADEIIEEPEKFLHVCDGKILATLFYEPSTRTRFSFEAAMLRLGGQVIGFSEPNSSS
VAKGESVADTIRTVGCYADIAAMRHPKEGAPAIAAMYSEIPVINAGDGSHQHPTQTLTDLLTIRSLKGDLSNLTIGCCGD
LKFGRTVHSLVKALSRYKNNKFVFMSPEELKIPDYIRKEILEKNNIEYKEVSKMEDAMAELDILYMTRVQRERFFNEDDY
VRLKDSYILDGEKMRYAKKDMMVLHPLPRVNEIAYEIDQDPRGCYFKQAKYGMYVRMALIAKLLGVR
>Mature_307_residues
MLKGRNLLDPMDFSLEELEEVFKLADEIIEEPEKFLHVCDGKILATLFYEPSTRTRFSFEAAMLRLGGQVIGFSEPNSSS
VAKGESVADTIRTVGCYADIAAMRHPKEGAPAIAAMYSEIPVINAGDGSHQHPTQTLTDLLTIRSLKGDLSNLTIGCCGD
LKFGRTVHSLVKALSRYKNNKFVFMSPEELKIPDYIRKEILEKNNIEYKEVSKMEDAMAELDILYMTRVQRERFFNEDDY
VRLKDSYILDGEKMRYAKKDMMVLHPLPRVNEIAYEIDQDPRGCYFKQAKYGMYVRMALIAKLLGVR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=45.0980392156863, Blast_Score=279, Evalue=2e-75,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=27.1565495207668, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI2367364, Length=306, Percent_Identity=45.0980392156863, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1786469, Length=163, Percent_Identity=32.5153374233129, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790703, Length=162, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=40.6832298136646, Blast_Score=237, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=44.299674267101, Blast_Score=272, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=45.7142857142857, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35008; Mature: 35008

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGRNLLDPMDFSLEELEEVFKLADEIIEEPEKFLHVCDGKILATLFYEPSTRTRFSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
AAMLRLGGQVIGFSEPNSSSVAKGESVADTIRTVGCYADIAAMRHPKEGAPAIAAMYSEI
HHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PVINAGDGSHQHPTQTLTDLLTIRSLKGDLSNLTIGCCGDLKFGRTVHSLVKALSRYKNN
CEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCEEEEECCCCHHHHHHHHHHHHHHHCCC
KFVFMSPEELKIPDYIRKEILEKNNIEYKEVSKMEDAMAELDILYMTRVQRERFFNEDDY
CEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VRLKDSYILDGEKMRYAKKDMMVLHPLPRVNEIAYEIDQDPRGCYFKQAKYGMYVRMALI
EEEECCEEECCHHHHHHHHCCEEECCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHH
AKLLGVR
HHHHCCC
>Mature Secondary Structure
MLKGRNLLDPMDFSLEELEEVFKLADEIIEEPEKFLHVCDGKILATLFYEPSTRTRFSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
AAMLRLGGQVIGFSEPNSSSVAKGESVADTIRTVGCYADIAAMRHPKEGAPAIAAMYSEI
HHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PVINAGDGSHQHPTQTLTDLLTIRSLKGDLSNLTIGCCGDLKFGRTVHSLVKALSRYKNN
CEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCEEEEECCCCHHHHHHHHHHHHHHHCCC
KFVFMSPEELKIPDYIRKEILEKNNIEYKEVSKMEDAMAELDILYMTRVQRERFFNEDDY
CEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VRLKDSYILDGEKMRYAKKDMMVLHPLPRVNEIAYEIDQDPRGCYFKQAKYGMYVRMALI
EEEECCEEECCHHHHHHHHCCEEECCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHH
AKLLGVR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA