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The map label for this gene is pyrI

Identifier: 170757312

GI number: 170757312

Start: 3518839

End: 3519279

Strand: Reverse

Name: pyrI

Synonym: CLD_1291

Alternate gene names: 170757312

Gene position: 3519279-3518839 (Counterclockwise)

Preceding gene: 170756348

Following gene: 170755484

Centisome position: 88.91

GC content: 24.49

Gene sequence:

>441_bases
ATGTTAACAATAAATAGTATAAAAAATGGTATAGTAATAGATCATATACAAGCAGGTCACGGTATAAAAATATTTAAATA
CTTAGGATTAGAAGAAGCTGATTATAGAGTTGCTCTTATAATGAATGCTGAAAGTTCAAAATTGGGGAAGAAAGATATAA
TAAAGATAGAAAATATCATGGAGATAGATTATAAAGTGTTAGGATTTATAGATCCTACAATAACAATAGATGTTATCGAA
AATGAAACAATAAAAGAAAAAATAAAATTAGAACTACCTAAGACAATAGAAAATGTTATAAAGTGTAAAAACCCACGTTG
CATAACATCTATAGAAAATTATATACCTAATGAATTTTACTTAGTAGATGAAGAAAATGGTGAATATAGATGTAAATATT
GTGATGAAATATATTCAGGGGGAGATATAAATAAATTATAA

Upstream 100 bases:

>100_bases
GAAATAGATCAAGACCCAAGAGGATGTTATTTTAAACAAGCTAAATATGGAATGTATGTAAGAATGGCTTTAATAGCAAA
ATTATTGGGGGTTAGATAAT

Downstream 100 bases:

>100_bases
AATTTATGGAGGTGTGCACAAATGAATGAATTACTAATTAAAAATATTAATATAATAGATTGGTGTCAAAACTTCCATGG
AGATGTTTATATAAAAAACG

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLTINSIKNGIVIDHIQAGHGIKIFKYLGLEEADYRVALIMNAESSKLGKKDIIKIENIMEIDYKVLGFIDPTITIDVIE
NETIKEKIKLELPKTIENVIKCKNPRCITSIENYIPNEFYLVDEENGEYRCKYCDEIYSGGDINKL

Sequences:

>Translated_146_residues
MLTINSIKNGIVIDHIQAGHGIKIFKYLGLEEADYRVALIMNAESSKLGKKDIIKIENIMEIDYKVLGFIDPTITIDVIE
NETIKEKIKLELPKTIENVIKCKNPRCITSIENYIPNEFYLVDEENGEYRCKYCDEIYSGGDINKL
>Mature_146_residues
MLTINSIKNGIVIDHIQAGHGIKIFKYLGLEEADYRVALIMNAESSKLGKKDIIKIENIMEIDYKVLGFIDPTITIDVIE
NETIKEKIKLELPKTIENVIKCKNPRCITSIENYIPNEFYLVDEENGEYRCKYCDEIYSGGDINKL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=141, Percent_Identity=35.4609929078014, Blast_Score=87, Evalue=4e-19,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_CLOB1 (A7FYJ1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385564.1
- ProteinModelPortal:   A7FYJ1
- SMR:   A7FYJ1
- STRING:   A7FYJ1
- GeneID:   5395465
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3277
- eggNOG:   COG1781
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   CBOT441770:CLB_3277-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 16747; Mature: 16747

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTINSIKNGIVIDHIQAGHGIKIFKYLGLEEADYRVALIMNAESSKLGKKDIIKIENIM
CEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHEEHHHHH
EIDYKVLGFIDPTITIDVIENETIKEKIKLELPKTIENVIKCKNPRCITSIENYIPNEFY
HCCEEEEEEECCEEEEEEECCCCHHHHEEEECCHHHHHHHHCCCCEEEHHHHHHCCCEEE
LVDEENGEYRCKYCDEIYSGGDINKL
EEECCCCCEEEEEHHHHHCCCCCCCC
>Mature Secondary Structure
MLTINSIKNGIVIDHIQAGHGIKIFKYLGLEEADYRVALIMNAESSKLGKKDIIKIENIM
CEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHEEHHHHH
EIDYKVLGFIDPTITIDVIENETIKEKIKLELPKTIENVIKCKNPRCITSIENYIPNEFY
HCCEEEEEEECCEEEEEEECCCCHHHHEEEECCHHHHHHHHCCCCEEEHHHHHHCCCEEE
LVDEENGEYRCKYCDEIYSGGDINKL
EEECCCCCEEEEEHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA