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The map label for this gene is pdhD [H]

Identifier: 170756245

GI number: 170756245

Start: 822449

End: 823837

Strand: Direct

Name: pdhD [H]

Synonym: CLD_0066

Alternate gene names: 170756245

Gene position: 822449-823837 (Clockwise)

Preceding gene: 170757826

Following gene: 170755220

Centisome position: 20.78

GC content: 31.32

Gene sequence:

>1389_bases
ATGAAGGATATTATAGTTATAGGTGGAGGCCCTGGTGGATATGTAGCGGCTATAAGAGCTGCACATTTAGGTGCTGATGT
ATCAGTTGTTGAGATGGATAGCTTTGGTGGAACATGCCTTAATAGAGGTTGCATACCAACAAAGACTTTATATAGAACAG
CAGAAATAATGAATATATTAAAACATATAGAAGACTTTGGAATAGATGCTGAAAACTATAATGTAAATATAGAGAAGGTT
CAGAAAAGAAAAAATAATGTAATAAAAGAATTAGTAGGTGGAGTCGAGAAATTACTAAAAGGAAATGGTGTTGAAATTAT
AAGAGGTAAGGCTTTCTTAAAAGATAAAAATACTGTTTTTGTAGAAACGAAAGATGGGCAAGTTACATTAGAAGGAAAAA
ACATAATTATAGCAACGGGCTCTAATGCTGAAATGCCAGCTATAAAAGGTATAGAGAATAAAAACATAATAGTAAGTGAT
GATATTTTAGAATTTGATAGAATTCCTAAAGATTTAGTAGTTTCAGGTGGCGGTGTTGTGGGAATGGAGTTTGCTAATAT
CTTTAAAGCTATGGGAAGTGAAGTAACAGTAATAGTTGCTAGGGATTCTATATTATATGATATTGATAGAGAAATAAGTA
AAAGGTATAAAGTTATAGCAAAAAAATCAGGAATAAATATATTAACATCAACTAAAATATTGGAGTTCGCTGAGGATGAT
AATAATGTAATTATTAAATGTGAAGGTAAAAAAGGTAAGCTTGAGTTAAATTGTGATATGGTTCTTATGGCTAAAGGTAG
AAGAGGAAACTTTACAGGTATGAACTTAGAAGAACTAGGGATTGAACATGATAAAAAGAAAATAATTGTAGATGATAATT
ATAAAACTAATATAGATGGAATATATGCAATTGGAGATGTAAATGGGATTTGTCTTTTAGCTCATGCAGCATCACATCAA
GGAATAGAGGTTGTAGAGCATATAATGGAAAATAAAGAATGCCATAAATCTGTGATTCCAAACTGTATATTTACATTCCC
TGAAATAGCTACTGTAGGTATGACAGAGGAAGAAATTAAGGCAAAGGGTATAGAGTATATTAAAAATAAATTCCTATTTG
GAGCTAATGGAAAAGCCTTGGCATTAGGTGAAGGTGAAGGTGTTGTAAAAGTTATATGTGAAAAGGAAAGTAAAAAGATT
TTAGGTATTCATATAATGGGGCCACATGCTTCAGATTTAATACATGAAGGTGTGGTAGCAATTGAAAAGGGTATGACAGT
AAATGATTTTAAAGAAGTAGTACATGCTCATCCAACATTAGGTGAAGCGTTCTATGAAGCAATGATGGGATTAAATAAGG
AGGCTATTCATAGTATAAACAAAAAATAA

Upstream 100 bases:

>100_bases
TTCAGAAGAGTTAAGATTTAAGAAAAGTAAAAATACAATTCTTATTTCTTAACTCTTAAATTTTAAAATGAATTAGATTA
TAGGGGGGAATAGTATTATT

Downstream 100 bases:

>100_bases
AATATTTTTAGTTGTTAATTTTAAAGAAGTTTGTTCAATTTGTTATAGCAGTAGAATTTACTTATAAGAATAGATACTAA
TGATAATTAAAATTAAATTA

Product: glycine cleavage system, dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVSVVEMDSFGGTCLNRGCIPTKTLYRTAEIMNILKHIEDFGIDAENYNVNIEKV
QKRKNNVIKELVGGVEKLLKGNGVEIIRGKAFLKDKNTVFVETKDGQVTLEGKNIIIATGSNAEMPAIKGIENKNIIVSD
DILEFDRIPKDLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
NNVIIKCEGKKGKLELNCDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIKAKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAMMGLNKEAIHSINKK

Sequences:

>Translated_462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVSVVEMDSFGGTCLNRGCIPTKTLYRTAEIMNILKHIEDFGIDAENYNVNIEKV
QKRKNNVIKELVGGVEKLLKGNGVEIIRGKAFLKDKNTVFVETKDGQVTLEGKNIIIATGSNAEMPAIKGIENKNIIVSD
DILEFDRIPKDLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
NNVIIKCEGKKGKLELNCDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIKAKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAMMGLNKEAIHSINKK
>Mature_462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVSVVEMDSFGGTCLNRGCIPTKTLYRTAEIMNILKHIEDFGIDAENYNVNIEKV
QKRKNNVIKELVGGVEKLLKGNGVEIIRGKAFLKDKNTVFVETKDGQVTLEGKNIIIATGSNAEMPAIKGIENKNIIVSD
DILEFDRIPKDLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
NNVIIKCEGKKGKLELNCDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIKAKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAMMGLNKEAIHSINKK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=38.646288209607, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=31.3465783664459, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=27.9735682819383, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI291045266, Length=427, Percent_Identity=25.5269320843091, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=24.4131455399061, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519430, Length=432, Percent_Identity=24.7685185185185, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=432, Percent_Identity=24.7685185185185, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=432, Percent_Identity=24.7685185185185, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277065, Length=432, Percent_Identity=24.7685185185185, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277071, Length=432, Percent_Identity=24.7685185185185, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786307, Length=447, Percent_Identity=36.241610738255, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=31.6916488222698, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=30.0230946882217, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081717, Length=448, Percent_Identity=26.3392857142857, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=38.3771929824561, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17557007, Length=467, Percent_Identity=29.1220556745182, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI71983429, Length=450, Percent_Identity=28.2222222222222, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=450, Percent_Identity=28.2222222222222, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=25.6728778467909, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=38.2845188284519, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=29.4243070362473, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6325166, Length=458, Percent_Identity=29.6943231441048, Blast_Score=172, Evalue=8e-44,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=38.7799564270153, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=28.1449893390192, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=28.1449893390192, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=28.1449893390192, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=27.9749478079332, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50421; Mature: 50421

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIIVIGGGPGGYVAAIRAAHLGADVSVVEMDSFGGTCLNRGCIPTKTLYRTAEIMNIL
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHH
KHIEDFGIDAENYNVNIEKVQKRKNNVIKELVGGVEKLLKGNGVEIIRGKAFLKDKNTVF
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCEEE
VETKDGQVTLEGKNIIIATGSNAEMPAIKGIENKNIIVSDDILEFDRIPKDLVVSGGGVV
EEECCCEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHCCCHHHEECCCCEE
GMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
HHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHEECCCCEEEEECEEEEEECCC
NNVIIKCEGKKGKLELNCDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDG
CCEEEEEECCCCEEEECCCEEEEECCCCCCCCCCCHHHHCCCCCCCEEEECCCCEECCCE
IYAIGDVNGICLLAHAASHQGIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIK
EEEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCHHHCCCCCCEECCCCHHHHH
AKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKILGIHIMGPHASDLIHEGVVA
HHHHHHHHHCEEECCCCCEEEEECCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHCHHH
IEKGMTVNDFKEVVHAHPTLGEAFYEAMMGLNKEAIHSINKK
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MKDIIVIGGGPGGYVAAIRAAHLGADVSVVEMDSFGGTCLNRGCIPTKTLYRTAEIMNIL
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHH
KHIEDFGIDAENYNVNIEKVQKRKNNVIKELVGGVEKLLKGNGVEIIRGKAFLKDKNTVF
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCEEE
VETKDGQVTLEGKNIIIATGSNAEMPAIKGIENKNIIVSDDILEFDRIPKDLVVSGGGVV
EEECCCEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHCCCHHHEECCCCEE
GMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
HHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHEECCCCEEEEECEEEEEECCC
NNVIIKCEGKKGKLELNCDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDG
CCEEEEEECCCCEEEECCCEEEEECCCCCCCCCCCHHHHCCCCCCCEEEECCCCEECCCE
IYAIGDVNGICLLAHAASHQGIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIK
EEEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCHHHCCCCCCEECCCCHHHHH
AKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKILGIHIMGPHASDLIHEGVVA
HHHHHHHHHCEEECCCCCEEEEECCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHCHHH
IEKGMTVNDFKEVVHAHPTLGEAFYEAMMGLNKEAIHSINKK
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]