Click here to switch to the map view.

The map label for this gene is lplA [H]

Identifier: 170755220

GI number: 170755220

Start: 824031

End: 825008

Strand: Direct

Name: lplA [H]

Synonym: CLD_0065

Alternate gene names: 170755220

Gene position: 824031-825008 (Clockwise)

Preceding gene: 170756245

Following gene: 170754232

Centisome position: 20.82

GC content: 26.99

Gene sequence:

>978_bases
ATGAAATATATTAATAATTCAAATATCGATCCATATTTTAATTTAGCAGCCGAAGAGTATTTTTTAAGACATAAAAATGA
TGAATACTTTATTTTATGGAGGGATGAACCTTGCGTTATAGTTGGAAAGAATCAAAATACATTATCAGAAATAGATATGG
ATTATATAGAAACTAACAAGACTAAAGTAGTAAGAAGACAGACAGGCGGTGGAGCTGTATTTCATGATTTAGGAAACTTA
AATTATACATTTATAGTTAAAGATGATGGTAAGACTTTTAATGACTTTGAAAGATTTTGTAAACCTATAATAGGAGTTTT
GAAGACATTAGGTGTTAAAGCTGAATTCGCAGGACGAAATGATTTATTAATAGATGGAAAAAAAATTTCTGGAACAGCTC
AATGTAAGTATAAAAATAGAGTGATGCATCATGGAACCCTTTTGTTTTCATCGGATATAGTAAACTTAAGTGGTGCCTTA
AAACCTAAAAAGATAAAATTTCAAGATAAGGCGGTTAAGTCAGTTATAAGCAGGATAACTAATATTTCCGAGCATTTAAA
TTTAGAAATTAATGTTTTAACCTTTAAAAATAAGATATTTGATTATATTTTAAAAAGTGAAAAAAATGCAAAGGTTATGC
CATTAATTCAAGATGAAATAGATGAAATAGAAAAAATTAAAAAAAGCAAATATGAAACTTGGGCTTGGAACTTTGGAAGC
TCTCCTAAATATGATTTTTACAATGAAGGAAAGTTTACAGGCGGGACTATAGAGTTAAATCTAAAAGTAGAAAAAGGGAT
AATTACAGATATAAAGATATTCGGAGATTTCTTTGGTGTTAAGGATGTAAAAGAAATAGAAGATTTATTAAAGGGTATTG
AACATAAAAAGGAAGAAATAAAGAATGTAATAAAGAATATTAATATAGATGAATATTTTTCCAGAATAACTATGGAAGAA
TTTCTAAGCTTATTCTAA

Upstream 100 bases:

>100_bases
TAAATTAATTATAAAGTAGATATACGGTGGAAAAAGAGGCCATAATAAGAGCATTAAAATTAGCTAAATGTTTATAAAGT
AGATAGATGGGGGAAAAATT

Downstream 100 bases:

>100_bases
TATAATAAAAAGGTATAAAAGAATCTATTTTTGAAGTGGTTAACGCTGTTAAATACTTAAAAATATATTCTTTTTTACAT
TTAATAGTTAGAAAAGTTTA

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKYINNSNIDPYFNLAAEEYFLRHKNDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNKTKVVRRQTGGGAVFHDLGNL
NYTFIVKDDGKTFNDFERFCKPIIGVLKTLGVKAEFAGRNDLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIVNLSGAL
KPKKIKFQDKAVKSVISRITNISEHLNLEINVLTFKNKIFDYILKSEKNAKVMPLIQDEIDEIEKIKKSKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGIITDIKIFGDFFGVKDVKEIEDLLKGIEHKKEEIKNVIKNINIDEYFSRITMEE
FLSLF

Sequences:

>Translated_325_residues
MKYINNSNIDPYFNLAAEEYFLRHKNDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNKTKVVRRQTGGGAVFHDLGNL
NYTFIVKDDGKTFNDFERFCKPIIGVLKTLGVKAEFAGRNDLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIVNLSGAL
KPKKIKFQDKAVKSVISRITNISEHLNLEINVLTFKNKIFDYILKSEKNAKVMPLIQDEIDEIEKIKKSKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGIITDIKIFGDFFGVKDVKEIEDLLKGIEHKKEEIKNVIKNINIDEYFSRITMEE
FLSLF
>Mature_325_residues
MKYINNSNIDPYFNLAAEEYFLRHKNDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNKTKVVRRQTGGGAVFHDLGNL
NYTFIVKDDGKTFNDFERFCKPIIGVLKTLGVKAEFAGRNDLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIVNLSGAL
KPKKIKFQDKAVKSVISRITNISEHLNLEINVLTFKNKIFDYILKSEKNAKVMPLIQDEIDEIEKIKKSKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGIITDIKIFGDFFGVKDVKEIEDLLKGIEHKKEEIKNVIKNINIDEYFSRITMEE
FLSLF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=251, Percent_Identity=30.6772908366534, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI21729884, Length=251, Percent_Identity=30.6772908366534, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI21729882, Length=251, Percent_Identity=30.6772908366534, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI21729880, Length=251, Percent_Identity=30.6772908366534, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI21729878, Length=251, Percent_Identity=30.6772908366534, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1790846, Length=319, Percent_Identity=31.0344827586207, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=29.5275590551181, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322415, Length=292, Percent_Identity=29.4520547945205, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28573464, Length=301, Percent_Identity=28.9036544850498, Blast_Score=121, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37594; Mature: 37594

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYINNSNIDPYFNLAAEEYFLRHKNDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCHHHHHCCC
TKVVRRQTGGGAVFHDLGNLNYTFIVKDDGKTFNDFERFCKPIIGVLKTLGVKAEFAGRN
EEEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCC
DLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIVNLSGALKPKKIKFQDKAVKSVISRIT
CEEEECCCCCCEEEEEHHCCHHCCCEEEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHH
NISEHLNLEINVLTFKNKIFDYILKSEKNAKVMPLIQDEIDEIEKIKKSKYETWAWNFGS
HHHHHCEEEEEEEEEHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEECCC
SPKYDFYNEGKFTGGTIELNLKVEKGIITDIKIFGDFFGVKDVKEIEDLLKGIEHKKEEI
CCCCCCCCCCCCCCCEEEEEEEEECCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KNVIKNINIDEYFSRITMEEFLSLF
HHHHHCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKYINNSNIDPYFNLAAEEYFLRHKNDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCHHHHHCCC
TKVVRRQTGGGAVFHDLGNLNYTFIVKDDGKTFNDFERFCKPIIGVLKTLGVKAEFAGRN
EEEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCC
DLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIVNLSGALKPKKIKFQDKAVKSVISRIT
CEEEECCCCCCEEEEEHHCCHHCCCEEEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHH
NISEHLNLEINVLTFKNKIFDYILKSEKNAKVMPLIQDEIDEIEKIKKSKYETWAWNFGS
HHHHHCEEEEEEEEEHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEECCC
SPKYDFYNEGKFTGGTIELNLKVEKGIITDIKIFGDFFGVKDVKEIEDLLKGIEHKKEEI
CCCCCCCCCCCCCCCEEEEEEEEECCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KNVIKNINIDEYFSRITMEEFLSLF
HHHHHCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]