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The map label for this gene is puuR [H]

Identifier: 170756136

GI number: 170756136

Start: 3460186

End: 3460755

Strand: Reverse

Name: puuR [H]

Synonym: CLD_1346

Alternate gene names: 170756136

Gene position: 3460755-3460186 (Counterclockwise)

Preceding gene: 170755519

Following gene: 170754784

Centisome position: 87.43

GC content: 24.91

Gene sequence:

>570_bases
ATGATAGATGATATAGGTAAAAAAATAAAAAAATTAAGAACTAATAAAAAATTAACTTTAAAAGAATTAAGTGAAAAAAC
AAACCTTTCAATAGGTTTTCTATCACAGTTAGAAAGAGGTCTAACAACTGTAGCTATAGATTCTTTAACTAAAATAGCTA
AAGAACTGGATGTAAGTTTGACATACTTTTTTCAAGCACCTAAAAAAAATAAAAAAATAGTTCTTAGAAGTTATGAGAAA
GAAGTATTACAAGTAGAAAACAATAGATTTATAAAATACAATCTTAGTAATGATTTAGAAGATAAGAGCTTTTTACCAAG
ATTAATAGAAATACTTCCTACAGATAGTAAAGAGAATATTATTTCTTATCAACATAAAGGAGAAGAATTTGTGTATGTAT
TAGAAGGTATACTTACATTATTTATTAATGATGATGAGAAGGAATTATATCCTGGAGATAGCGCGCATTATGATTCAAGT
ATAAATCATAATTGGGCAAATTATACTAGTAAACCTGTAAGATTGTTAACAGTTCATACTCCTAATATGTTTAAGGACGA
AAAATATTAA

Upstream 100 bases:

>100_bases
TAAAATATATGCGTATACACATATATTTAAAGTTAAGTATTACTAATTTTCTGTGATATAATAATATATTATATAAAATT
AGTAATTAAGGAGATTAATT

Downstream 100 bases:

>100_bases
AATTTAATAAAGTAGATTTTATAAAACAATAGTTTGGGGGGCGGTTTACTTGAAGAAGAGCACTATTGTTAACATTCAAA
AATTTTCAGTTCATGATGGG

Product: DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MIDDIGKKIKKLRTNKKLTLKELSEKTNLSIGFLSQLERGLTTVAIDSLTKIAKELDVSLTYFFQAPKKNKKIVLRSYEK
EVLQVENNRFIKYNLSNDLEDKSFLPRLIEILPTDSKENIISYQHKGEEFVYVLEGILTLFINDDEKELYPGDSAHYDSS
INHNWANYTSKPVRLLTVHTPNMFKDEKY

Sequences:

>Translated_189_residues
MIDDIGKKIKKLRTNKKLTLKELSEKTNLSIGFLSQLERGLTTVAIDSLTKIAKELDVSLTYFFQAPKKNKKIVLRSYEK
EVLQVENNRFIKYNLSNDLEDKSFLPRLIEILPTDSKENIISYQHKGEEFVYVLEGILTLFINDDEKELYPGDSAHYDSS
INHNWANYTSKPVRLLTVHTPNMFKDEKY
>Mature_189_residues
MIDDIGKKIKKLRTNKKLTLKELSEKTNLSIGFLSQLERGLTTVAIDSLTKIAKELDVSLTYFFQAPKKNKKIVLRSYEK
EVLQVENNRFIKYNLSNDLEDKSFLPRLIEILPTDSKENIISYQHKGEEFVYVLEGILTLFINDDEKELYPGDSAHYDSS
INHNWANYTSKPVRLLTVHTPNMFKDEKY

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=181, Percent_Identity=25.9668508287293, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 22007; Mature: 22007

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDDIGKKIKKLRTNKKLTLKELSEKTNLSIGFLSQLERGLTTVAIDSLTKIAKELDVSL
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEE
TYFFQAPKKNKKIVLRSYEKEVLQVENNRFIKYNLSNDLEDKSFLPRLIEILPTDSKENI
EEEEECCCCCCEEEEHHHHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCE
ISYQHKGEEFVYVLEGILTLFINDDEKELYPGDSAHYDSSINHNWANYTSKPVRLLTVHT
EEHHCCCCHHHHHHHHHHEEEECCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
PNMFKDEKY
CCCCCCCCC
>Mature Secondary Structure
MIDDIGKKIKKLRTNKKLTLKELSEKTNLSIGFLSQLERGLTTVAIDSLTKIAKELDVSL
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEE
TYFFQAPKKNKKIVLRSYEKEVLQVENNRFIKYNLSNDLEDKSFLPRLIEILPTDSKENI
EEEEECCCCCCEEEEHHHHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCE
ISYQHKGEEFVYVLEGILTLFINDDEKELYPGDSAHYDSSINHNWANYTSKPVRLLTVHT
EEHHCCCCHHHHHHHHHHEEEECCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
PNMFKDEKY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]