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The map label for this gene is nadE

Identifier: 170756133

GI number: 170756133

Start: 660305

End: 662221

Strand: Reverse

Name: nadE

Synonym: CLD_0212

Alternate gene names: 170756133

Gene position: 662221-660305 (Counterclockwise)

Preceding gene: 170755147

Following gene: 170755932

Centisome position: 16.73

GC content: 29.79

Gene sequence:

>1917_bases
ATGAATAATTCACAATACTACATAAGAGTATCTACTGCTTGTCCTGTAACTAATGTGGCAGATATAGATTTTAATATAGA
AAACATAAAAAAGTGTATAGATATATGTATAGAAAAAAAATCAAAATTAATAGTTTTTCCAGAACTTTGTATAACTTCTT
ATACTTGTGGAGAGCTTTTTTCACAAAGCCTATTAATGTCCAAAGCTCTAGATGGAATACATAATATATGTAAATACTCT
ATAGATAAAGATGTTTTAATAGCTATAGGAGCACCTTTATTATATAAAAATAGTCTTTATAATTGTGCTGTTGTAATATT
TGGAGGAAAAATACTAGGTATTGTCCCTAAAAGTTACTTACCTAATTATTCAGAGTTCTATGAGAAAAGATGGTTTACTG
AAGGATATAAAATAAAAAGTGAAAGAATTAATTTACCATTCCAAGAGAACATTCCTTTTGGAGTTAACCTTATTTTTTCT
GATAAAATTTTCAAATTTGCTTTCGAAGTATGTGAAGATCTTTGGGTTACAATTCCACCTAGTAGTTATCTTGCATTAAT
GGGAGCTAATATTATTGGAAATTTATCTGCCTCTAATGAAATAGTTAGTAAATCCGATTATAGACGAAATTTAGTTGCAT
CTCAAAGTGGTAGATGCTTAGCATCTTATGTATATTCCTCATCAGGGGTATATGAGTCTTCTACGGATTTAGTATTTAGC
GGACACTTATTAATTGGAGAAAATGGCTCTATATTAAAAGAAAACAAAAGATTCCAAAGAGAAAATGAAGTTATTACAAG
TATTATAGATATAGATAAAATTAATAGCGAAAGACTTAAAAATGTGAGCTTTACAGATAACTCAATGAATATGAACTTAG
AATTAGAAGAAATAACATTTCAATTTGCAATTAACGATGTTGGTGACTTTGATAGACCTATAGACAAATACCCTTTTGTT
CCTTCTAATGAAGAAAAAAGAGCAGTAAGATGCAAAGAAATATTTAATATACAAACCTCAGCTCTAGCTAAAAGACTTGA
TCATACTAATATGAAAAAAGCAGTTATAGGTATCTCCGGTGGATTAGATTCTACCTTAGCTTTATTAGTTATAGCTAAAA
CCTTTGATAAATTAAACATTCCTAGAGAAAACATAATAACTATAACAATGCCTGGTTTTGGAACTACAGATAGAACTTAT
AATAATGCTGTAAGTCTATGTAAACACTTAAATACAACCTTAAAAGAAATAAATATAGTAGATGCAGCTCTGCAACATTT
TAAAGATATAGGCCATGACAAGGATATACATGATGTTACCTATGAAAATGTTCAAGCAAGAGAGAGAACTCAAATACTTA
TGGATATAGCCAATAAAGAAGGTGGCTTAGTTATAGGAACCGGTGATTTATCTGAATTAGCTTTAGGTTGGTGTACATAT
AATGGAGATCATATGTCCATGTACAGCGTAAATTGTTCTATTCCTAAGACCTTAGTAAGATATCTGGTGAGATATGTAGC
TGAAAATGAAGTAGCAAAAGAAATATCAGAAATACTTATAGATATTTTAGATACACCTGTAAGTCCTGAACTATTACCTA
AGGATAAAGAGGGCAACATTGTTCAAAAAACTGAAGATATAGTAGGACCTTATGAGTTGCATGATTTCTTTCTTTATTAT
TTTATTCGTCAAGGAGCTACTCCAGATAAAATAAAACAATTAGCTAAAATAACTTTTAAAGATTCCTATGATAAAGAAAC
TATTGACAAATGGTTCTCTTATTTTATAAGAAGATTCTTTACTCAACAATTTAAACGTTCCGCAGTACCAGACGGTCCTA
AAGTTGGAACTATAAGTTTATCCCCTAGAGGAGATTGGAGAATGCCATCAGACGCAAGTTTTAACCTTTGGAAATAA

Upstream 100 bases:

>100_bases
TTGCATAATTTTTTTATTAATATTTTATATTAAAGTTTAATTATTTACCTTTAATTTGATATAATCATAGGGTAATCATA
TTTTAAATGGAGGATTTTAT

Downstream 100 bases:

>100_bases
AAAAACAAAATAACATAATATATTTACTTTTGAAATTTAATAACGTGTCTCAAAATAAATTTAATTTTGAGGCACTATCT
ATTTTTATAACTATCCTAAA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MNNSQYYIRVSTACPVTNVADIDFNIENIKKCIDICIEKKSKLIVFPELCITSYTCGELFSQSLLMSKALDGIHNICKYS
IDKDVLIAIGAPLLYKNSLYNCAVVIFGGKILGIVPKSYLPNYSEFYEKRWFTEGYKIKSERINLPFQENIPFGVNLIFS
DKIFKFAFEVCEDLWVTIPPSSYLALMGANIIGNLSASNEIVSKSDYRRNLVASQSGRCLASYVYSSSGVYESSTDLVFS
GHLLIGENGSILKENKRFQRENEVITSIIDIDKINSERLKNVSFTDNSMNMNLELEEITFQFAINDVGDFDRPIDKYPFV
PSNEEKRAVRCKEIFNIQTSALAKRLDHTNMKKAVIGISGGLDSTLALLVIAKTFDKLNIPRENIITITMPGFGTTDRTY
NNAVSLCKHLNTTLKEINIVDAALQHFKDIGHDKDIHDVTYENVQARERTQILMDIANKEGGLVIGTGDLSELALGWCTY
NGDHMSMYSVNCSIPKTLVRYLVRYVAENEVAKEISEILIDILDTPVSPELLPKDKEGNIVQKTEDIVGPYELHDFFLYY
FIRQGATPDKIKQLAKITFKDSYDKETIDKWFSYFIRRFFTQQFKRSAVPDGPKVGTISLSPRGDWRMPSDASFNLWK

Sequences:

>Translated_638_residues
MNNSQYYIRVSTACPVTNVADIDFNIENIKKCIDICIEKKSKLIVFPELCITSYTCGELFSQSLLMSKALDGIHNICKYS
IDKDVLIAIGAPLLYKNSLYNCAVVIFGGKILGIVPKSYLPNYSEFYEKRWFTEGYKIKSERINLPFQENIPFGVNLIFS
DKIFKFAFEVCEDLWVTIPPSSYLALMGANIIGNLSASNEIVSKSDYRRNLVASQSGRCLASYVYSSSGVYESSTDLVFS
GHLLIGENGSILKENKRFQRENEVITSIIDIDKINSERLKNVSFTDNSMNMNLELEEITFQFAINDVGDFDRPIDKYPFV
PSNEEKRAVRCKEIFNIQTSALAKRLDHTNMKKAVIGISGGLDSTLALLVIAKTFDKLNIPRENIITITMPGFGTTDRTY
NNAVSLCKHLNTTLKEINIVDAALQHFKDIGHDKDIHDVTYENVQARERTQILMDIANKEGGLVIGTGDLSELALGWCTY
NGDHMSMYSVNCSIPKTLVRYLVRYVAENEVAKEISEILIDILDTPVSPELLPKDKEGNIVQKTEDIVGPYELHDFFLYY
FIRQGATPDKIKQLAKITFKDSYDKETIDKWFSYFIRRFFTQQFKRSAVPDGPKVGTISLSPRGDWRMPSDASFNLWK
>Mature_638_residues
MNNSQYYIRVSTACPVTNVADIDFNIENIKKCIDICIEKKSKLIVFPELCITSYTCGELFSQSLLMSKALDGIHNICKYS
IDKDVLIAIGAPLLYKNSLYNCAVVIFGGKILGIVPKSYLPNYSEFYEKRWFTEGYKIKSERINLPFQENIPFGVNLIFS
DKIFKFAFEVCEDLWVTIPPSSYLALMGANIIGNLSASNEIVSKSDYRRNLVASQSGRCLASYVYSSSGVYESSTDLVFS
GHLLIGENGSILKENKRFQRENEVITSIIDIDKINSERLKNVSFTDNSMNMNLELEEITFQFAINDVGDFDRPIDKYPFV
PSNEEKRAVRCKEIFNIQTSALAKRLDHTNMKKAVIGISGGLDSTLALLVIAKTFDKLNIPRENIITITMPGFGTTDRTY
NNAVSLCKHLNTTLKEINIVDAALQHFKDIGHDKDIHDVTYENVQARERTQILMDIANKEGGLVIGTGDLSELALGWCTY
NGDHMSMYSVNCSIPKTLVRYLVRYVAENEVAKEISEILIDILDTPVSPELLPKDKEGNIVQKTEDIVGPYELHDFFLYY
FIRQGATPDKIKQLAKITFKDSYDKETIDKWFSYFIRRFFTQQFKRSAVPDGPKVGTISLSPRGDWRMPSDASFNLWK

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=603, Percent_Identity=23.2172470978441, Blast_Score=126, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17538756, Length=612, Percent_Identity=23.202614379085, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321865, Length=614, Percent_Identity=25.2442996742671, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24641841, Length=608, Percent_Identity=21.7105263157895, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24641843, Length=608, Percent_Identity=21.7105263157895, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 72313; Mature: 72313

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSQYYIRVSTACPVTNVADIDFNIENIKKCIDICIEKKSKLIVFPELCITSYTCGELF
CCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHH
SQSLLMSKALDGIHNICKYSIDKDVLIAIGAPLLYKNSLYNCAVVIFGGKILGIVPKSYL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCEEEEEEECCEEEEECCHHHC
PNYSEFYEKRWFTEGYKIKSERINLPFQENIPFGVNLIFSDKIFKFAFEVCEDLWVTIPP
CCHHHHHHHHHCCCCEEEECCEECCCHHCCCCCCEEEEECHHHHHHHHHHHHHCEEECCC
SSYLALMGANIIGNLSASNEIVSKSDYRRNLVASQSGRCLASYVYSSSGVYESSTDLVFS
HHHHHHHCCHHHCCCCCCCCHHCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCEEEE
GHLLIGENGSILKENKRFQRENEVITSIIDIDKINSERLKNVSFTDNSMNMNLELEEITF
EEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEECCCCCEEEEEEHHEEE
QFAINDVGDFDRPIDKYPFVPSNEEKRAVRCKEIFNIQTSALAKRLDHTNMKKAVIGISG
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECC
GLDSTLALLVIAKTFDKLNIPRENIITITMPGFGTTDRTYNNAVSLCKHLNTTLKEINIV
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DAALQHFKDIGHDKDIHDVTYENVQARERTQILMDIANKEGGLVIGTGDLSELALGWCTY
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHEEEEEE
NGDHMSMYSVNCSIPKTLVRYLVRYVAENEVAKEISEILIDILDTPVSPELLPKDKEGNI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
VQKTEDIVGPYELHDFFLYYFIRQGATPDKIKQLAKITFKDSYDKETIDKWFSYFIRRFF
EEHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TQQFKRSAVPDGPKVGTISLSPRGDWRMPSDASFNLWK
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNSQYYIRVSTACPVTNVADIDFNIENIKKCIDICIEKKSKLIVFPELCITSYTCGELF
CCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHH
SQSLLMSKALDGIHNICKYSIDKDVLIAIGAPLLYKNSLYNCAVVIFGGKILGIVPKSYL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCEEEEEEECCEEEEECCHHHC
PNYSEFYEKRWFTEGYKIKSERINLPFQENIPFGVNLIFSDKIFKFAFEVCEDLWVTIPP
CCHHHHHHHHHCCCCEEEECCEECCCHHCCCCCCEEEEECHHHHHHHHHHHHHCEEECCC
SSYLALMGANIIGNLSASNEIVSKSDYRRNLVASQSGRCLASYVYSSSGVYESSTDLVFS
HHHHHHHCCHHHCCCCCCCCHHCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCEEEE
GHLLIGENGSILKENKRFQRENEVITSIIDIDKINSERLKNVSFTDNSMNMNLELEEITF
EEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEECCCCCEEEEEEHHEEE
QFAINDVGDFDRPIDKYPFVPSNEEKRAVRCKEIFNIQTSALAKRLDHTNMKKAVIGISG
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECC
GLDSTLALLVIAKTFDKLNIPRENIITITMPGFGTTDRTYNNAVSLCKHLNTTLKEINIV
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DAALQHFKDIGHDKDIHDVTYENVQARERTQILMDIANKEGGLVIGTGDLSELALGWCTY
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHEEEEEE
NGDHMSMYSVNCSIPKTLVRYLVRYVAENEVAKEISEILIDILDTPVSPELLPKDKEGNI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
VQKTEDIVGPYELHDFFLYYFIRQGATPDKIKQLAKITFKDSYDKETIDKWFSYFIRRFF
EEHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TQQFKRSAVPDGPKVGTISLSPRGDWRMPSDASFNLWK
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]