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The map label for this gene is alaS

Identifier: 170755913

GI number: 170755913

Start: 2732572

End: 2735211

Strand: Reverse

Name: alaS

Synonym: CLD_2001

Alternate gene names: 170755913

Gene position: 2735211-2732572 (Counterclockwise)

Preceding gene: 170756779

Following gene: 170754442

Centisome position: 69.1

GC content: 30.11

Gene sequence:

>2640_bases
ATGGAAAGAATGGGTTTAAATGAAATAAGAGAAGAATATCTAAAGTTTTTTGAAAGTAAAGCACATTTAAGACTACCGAG
CTTTTCTTTAGTGCCTAAAAATGATAAAAGTTTACTTTTAATAAATGCAGGAATGGCCCCTTTAAAGCCTTATTTTACAG
GGCTTCAAGTTCCACCAAATAAAAGGGTGACTACTTGTCAAAAATGTGTAAGAACTGGGGATATTGAAAATGTAGGTAAA
ACTTCAAGACATGGTACTTTCTTTGAAATGATGGGAAATTTTTCTTTTGGAGATTATTTTAAGAAAGAAATTATTCCTTG
GGCTTGGGAATTTATTACAGAGATTTTAAAATTGCCTAAAGACAAATTATATGTGACTATATATGAAGACGATGATGAAG
CTTTAGACATATGGGTAAATAAAACAGATGTGGATCCTAAAAGAATATTTAGATTAGGAAAAGAAGATAATTTTTGGGAA
CATGGCTTAGGACCTTGTGGACCTTGTTCAGAAATTCACTTTGACAGGGGAGCAGGAGAGGTTAAAACTTCGGAAGAATT
TGTAAAAGCTAGTGATGAGGATAAAATAGTAGAATTTTGGAACTTAGTATTTACTCAATTTGATAAGGATGAAGAAGGAA
ATTATAATAAATTAGCTAATCCTAATATAGATACAGGTATGGGACTTGAAAGAATGGCTACTATAATGCAAAATGTGGAT
ACTATATTTGAAGTGGATACAATAAAAGCTGTATTAGATAAGGTTTGTGAACTTTCAGGAGCTAATTACAAAGAAGATAG
AGTAAAAGATATATCTATAAGAATAATAACAGATCATATAAGAAGTATTACTTTTATGATAAGCGATGGTATATTACCTT
CTAATGAAGGAAGAGGATATGTTCTTAGAAGACTTTTAAGAAGAGCTGCAAGACATGGGAAAACCTTAGGAATAAAAAAT
ACATTTTTACATAACTTAACAGACATAGTTATAGAAAATTGTTATAAAAATTATCCAGAATTAGAAGAAAAAAGAGAATA
TATAAAGAAAGTTATAAAATTAGAAGAAGAAAGATTTGACGAAACTATAGATGCTGGAATGCAAATACTAAATGATTATA
TAAAAGAAGTTAAGAATAATAATCATAAAGTTCTTTCAGGAGATAAAGCTTTCAAACTATACGATACTTATGGATTCCCA
GTAGAATTAACGGAAGAAATATTAGAAGAGGAAGGTATAAGTATAGATAAAGAAGGCTTTAATAAAGAAATGAAGGAGCA
AAGAGAAAGAGCTAGATCCGCTAGAGAAGAAACTAATTATATGGGGTCAGAGGATACTATATTAAATAAAATAGATTTAA
ATATAAATACAGACTTTGAAGGTTACGATAAATTAGAAGTAAAATCAAAAGTGGCTGTGATTATAAAAGATGAAGAATTC
AAAAATGGAATGGAAAAAGGAAATGAAGGGGTAATAGTAACTTATAATACTCCATTTTATGCAGAAATGGGTGGTCAAAT
AGGAGATACAGGTATTATATATAATGATAGTTTTAAAGCAGAAGTTATAGATTGTAAAAAGAACATATCAGGTAAAGTAC
TTCATTTTGTAAAAATTTTAGATGGAAAAGTTGCTTTAGAAGATCAAGTTATATTAAAAGTAAATGAAGAAAGAAGAAAT
AATATAAGAAAAAATCATACAGCAACCCATATACTACATGCTGCTTTAATAAAAGTGGTAGGAGATCATGTACAACAATC
AGGTTCCTATGTGGATGATGAAAGACTAAGATTTGACTTTAGTCATTTTGAAGCAGTATCAGAGGATGAATTAAAAGAAG
TAGAGAAGATAGTAAATAAAGAGATAATGAAAGCCAATGCTGTAAATACTAAAGTAATGAATATAGAAGAAGCAAAAGAA
CAAGGGGCTATTGCATTATTCGATAATAAATATAAGGATGATGTTAGAGTAGTTTCTGTAGGAAATTTTAGTAAAGAATT
ATGTGGTGGAACTCATGTAAGTAATAGTGGCCAGATTGGAATGTTTAAAATAGTTTCTGAGGCAGGGGTTGCAGCTGGCA
TAAGAAGAATAGAGGCAGTAACGGCTTTTAAAGCTATGGAATATGTAGATCATAAAAATAACATATTAAAAGAAGCAGCT
CAAATATTAAAATGTAACGAAAAGGAATTACTAAATAAATTAAATCATCAAGTTCTAGAAATGAAAGAAAAGGAAAAGGA
AATAGAAGCTTTAAAATTAAAATTAGCAAGTGGTGCAGAAGATGAAATATTAAATAACATAAGAGAAATAAAAGGTGTCA
AAGTAGCATCAGCTGCAGTAAAGGATATAGATGGGAATGCCCTAAGAGATTTAGGCGATAAAATAAGAGATAATATGCAA
AGTGGAGTAGTTGTTCTAGGAAGTAACTATAAAGGTAAAGTTTTATTTGTTGCTATGGCAACTAAGGATACAGTAGCTAA
AGGAGTGCATTGTGGAAAAATAATTAAAGAGGTTGCAACTATAGCCGGTGGTGGTGGTGGTGGAAGACCAGACATGGCAC
AGGCAGGAGGTAAAGATCCAAATAAATTAGAAGATGCTATAAAAACAGTAGAAACTGTAGTTGAAAGTTTAGTTAAATAG

Upstream 100 bases:

>100_bases
TATTTAAATTAGGGTGGTACCGCGGAATATAACTTTCGTCCCTTTAGGGATTGAAGGTTTTTTTTATTGTAAAAATATAA
ATATAAAGGAGAGATAAACC

Downstream 100 bases:

>100_bases
TATACTTTTTAATAAATATGTCTTATAATATAATTAAAGAACATAAAAAACACTCAATACTGGTTGCTCCAAAGAAAGTT
GCAATCCTTAAAGATTGTAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPNKRVTTCQKCVRTGDIENVGK
TSRHGTFFEMMGNFSFGDYFKKEIIPWAWEFITEILKLPKDKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWE
HGLGPCGPCSEIHFDRGAGEVKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
TIFEVDTIKAVLDKVCELSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGYVLRRLLRRAARHGKTLGIKN
TFLHNLTDIVIENCYKNYPELEEKREYIKKVIKLEEERFDETIDAGMQILNDYIKEVKNNNHKVLSGDKAFKLYDTYGFP
VELTEEILEEEGISIDKEGFNKEMKEQRERARSAREETNYMGSEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
KNGMEKGNEGVIVTYNTPFYAEMGGQIGDTGIIYNDSFKAEVIDCKKNISGKVLHFVKILDGKVALEDQVILKVNEERRN
NIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDFSHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKE
QGAIALFDNKYKDDVRVVSVGNFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILNNIREIKGVKVASAAVKDIDGNALRDLGDKIRDNMQ
SGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVATIAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK

Sequences:

>Translated_879_residues
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPNKRVTTCQKCVRTGDIENVGK
TSRHGTFFEMMGNFSFGDYFKKEIIPWAWEFITEILKLPKDKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWE
HGLGPCGPCSEIHFDRGAGEVKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
TIFEVDTIKAVLDKVCELSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGYVLRRLLRRAARHGKTLGIKN
TFLHNLTDIVIENCYKNYPELEEKREYIKKVIKLEEERFDETIDAGMQILNDYIKEVKNNNHKVLSGDKAFKLYDTYGFP
VELTEEILEEEGISIDKEGFNKEMKEQRERARSAREETNYMGSEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
KNGMEKGNEGVIVTYNTPFYAEMGGQIGDTGIIYNDSFKAEVIDCKKNISGKVLHFVKILDGKVALEDQVILKVNEERRN
NIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDFSHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKE
QGAIALFDNKYKDDVRVVSVGNFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILNNIREIKGVKVASAAVKDIDGNALRDLGDKIRDNMQ
SGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVATIAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK
>Mature_879_residues
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPNKRVTTCQKCVRTGDIENVGK
TSRHGTFFEMMGNFSFGDYFKKEIIPWAWEFITEILKLPKDKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWE
HGLGPCGPCSEIHFDRGAGEVKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
TIFEVDTIKAVLDKVCELSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGYVLRRLLRRAARHGKTLGIKN
TFLHNLTDIVIENCYKNYPELEEKREYIKKVIKLEEERFDETIDAGMQILNDYIKEVKNNNHKVLSGDKAFKLYDTYGFP
VELTEEILEEEGISIDKEGFNKEMKEQRERARSAREETNYMGSEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
KNGMEKGNEGVIVTYNTPFYAEMGGQIGDTGIIYNDSFKAEVIDCKKNISGKVLHFVKILDGKVALEDQVILKVNEERRN
NIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDFSHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKE
QGAIALFDNKYKDDVRVVSVGNFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILNNIREIKGVKVASAAVKDIDGNALRDLGDKIRDNMQ
SGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVATIAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=970, Percent_Identity=33.6082474226804, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI38569417, Length=768, Percent_Identity=29.1666666666667, Blast_Score=356, Evalue=5e-98,
Organism=Escherichia coli, GI1789048, Length=870, Percent_Identity=42.4137931034483, Blast_Score=716, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=985, Percent_Identity=33.8071065989848, Blast_Score=487, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17536681, Length=758, Percent_Identity=32.8496042216359, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324911, Length=952, Percent_Identity=35.7142857142857, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24582809, Length=972, Percent_Identity=32.9218106995885, Blast_Score=487, Evalue=1e-137,
Organism=Drosophila melanogaster, GI45552267, Length=972, Percent_Identity=32.9218106995885, Blast_Score=487, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24658214, Length=778, Percent_Identity=28.1491002570694, Blast_Score=290, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 99232; Mature: 99232

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPN
CCCCCHHHHHHHHHHHHHCCCCEECCCCEECCCCCCEEEEEECCCCCCCHHHCCCCCCCC
KRVTTCQKCVRTGDIENVGKTSRHGTFFEMMGNFSFGDYFKKEIIPWAWEFITEILKLPK
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHCCC
DKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWEHGLGPCGPCSEIHFDRGAGE
CCEEEEEEECCCCEEEEEEECCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
VKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TIFEVDTIKAVLDKVCELSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHEEHEEECCCCCCCCCCHH
VLRRLLRRAARHGKTLGIKNTFLHNLTDIVIENCYKNYPELEEKREYIKKVIKLEEERFD
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ETIDAGMQILNDYIKEVKNNNHKVLSGDKAFKLYDTYGFPVELTEEILEEEGISIDKEGF
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHC
NKEMKEQRERARSAREETNYMGSEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEECCEEEEEEECHHH
KNGMEKGNEGVIVTYNTPFYAEMGGQIGDTGIIYNDSFKAEVIDCKKNISGKVLHFVKIL
HHHHHCCCCCEEEEECCCCHHHCCCCCCCCEEEECCCCCEEHHHHHCCCCHHHHHHHHHH
DGKVALEDQVILKVNEERRNNIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDF
CCCEEECCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECH
SHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKEQGAIALFDNKYKDDVRVVSV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCEEEECCCCCCCEEEEEE
GNFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
CCCHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILNNIREIKGVKVASAAV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
KDIDGNALRDLGDKIRDNMQSGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVAT
HCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHHH
IAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK
HCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPN
CCCCCHHHHHHHHHHHHHCCCCEECCCCEECCCCCCEEEEEECCCCCCCHHHCCCCCCCC
KRVTTCQKCVRTGDIENVGKTSRHGTFFEMMGNFSFGDYFKKEIIPWAWEFITEILKLPK
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHCCC
DKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWEHGLGPCGPCSEIHFDRGAGE
CCEEEEEEECCCCEEEEEEECCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
VKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TIFEVDTIKAVLDKVCELSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHEEHEEECCCCCCCCCCHH
VLRRLLRRAARHGKTLGIKNTFLHNLTDIVIENCYKNYPELEEKREYIKKVIKLEEERFD
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ETIDAGMQILNDYIKEVKNNNHKVLSGDKAFKLYDTYGFPVELTEEILEEEGISIDKEGF
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHC
NKEMKEQRERARSAREETNYMGSEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEECCEEEEEEECHHH
KNGMEKGNEGVIVTYNTPFYAEMGGQIGDTGIIYNDSFKAEVIDCKKNISGKVLHFVKIL
HHHHHCCCCCEEEEECCCCHHHCCCCCCCCEEEECCCCCEEHHHHHCCCCHHHHHHHHHH
DGKVALEDQVILKVNEERRNNIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDF
CCCEEECCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECH
SHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKEQGAIALFDNKYKDDVRVVSV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCEEEECCCCCCCEEEEEE
GNFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
CCCHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILNNIREIKGVKVASAAV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
KDIDGNALRDLGDKIRDNMQSGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVAT
HCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHHH
IAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK
HCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA