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The map label for this gene is tuf-1

Identifier: 170755852

GI number: 170755852

Start: 3778965

End: 3780158

Strand: Reverse

Name: tuf-1

Synonym: CLD_1022

Alternate gene names: 170755852

Gene position: 3780158-3778965 (Counterclockwise)

Preceding gene: 170756221

Following gene: 170756211

Centisome position: 95.5

GC content: 36.6

Gene sequence:

>1194_bases
ATGGCAAAAGCAAAATTTGAAAGAAGTAAACCTCATGTAAACATAGGAACAATAGGTCACGTAGACCACGGTAAGACAAC
ATTAACAGCAGCTATTACAACAGTATTAGCACAAAAAGGAGGAGCTTCAGCAACAAAATATGACGAAATAGATAAAGCTC
CAGAAGAAAAAGAAAGAGGAATCACAATAAATACATCACACGTAGAGTATGAAACAGCAAACAGACACTACGCACACGTA
GACTGCCCAGGACACGCAGACTATGTAAAGAACATGATAACAGGAGCAGCACAAATGGATGGAGCAATCTTAGTTGTATC
AGCAGCAGATGGTCCAATGCCACAAACAAGAGAACATATACTACTAGCATCAAGAGTTGGAGTACAATATATAGTAGTAT
TCTTAAATAAAGCTGACCAAGTAGACGATCCAGAACTAATAGAATTAGTTGAAATGGAAGTAAGAGAATTACTAAATGAA
TATGGATTCCCAGGAGATGATACTCCAATAGTAGTAGGATCAGCATTAGAAGTATTAGAAAACCAAGACAATGCAGAAAA
AACAAAATGCATAGATGAATTAATGGAAGCAATAGATAGCTATATACCAACACCAGAAAGAGCAACAGATCAACCATTCT
TAATGCCAGTAGAAGACGTATTTACAATAACAGGAAGAGGAACAGTTGCAACAGGAAGAGTTGAAAGAGGAGTCCTACAT
ACTGGAGATGAAGTAGAATTAATAGGAATGAAACAAGAAGTATCAAAAACAGTATGTACAGGAATAGAAATGTTCAGAAA
AATACTTGATGAAGCAATGGCAGGAGACAACATAGGAGCATTATTAAGAGGTATCCAAAGAGACGAAATCCAAAGAGGTC
AAGTATTAGCAAAACCAGGTTCAGTAACACCACACAAAAAATTCGTAGGTCAAGTATACGTATTAAAGAAAGAAGAAGGT
GGAAGACATACACCATTCTTTAACGGATATAGACCACAATTCTACTTCAGAACAACAGACGTTACAGGATCAATCAACTT
ACCAGAAGGAGTAGAAATGGTAATGCCTGGAGATCATATAGATATGGCAGTAGAATTAATCACACCAGTAGCAATGCATG
AAAACTTAAGATTCGCTATCAGAGAAGGTGGAAGAACAGTAGGTTCAGGAGTTGTTACAACAATATCTGAATAA

Upstream 100 bases:

>100_bases
TGATCATTATGAAGAAGTTCCAAAGAGCATTCAAGATAAAGTAATTGGAGAAAGAAAATAATATTATAATTATATAAGCA
CAAAAAAGGAGGAAATAAAA

Downstream 100 bases:

>100_bases
TTTAAATAGTTAGGCTAACATATAAATATATAATATCTAAAAGGAAAGAGGACGCTCTTTCCTTTTGTTTATAAAAATAA
GTTTAATACATATAAATTTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQKGGASATKYDEIDKAPEEKERGITINTSHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLASRVGVQYIVVFLNKADQVDDPELIELVEMEVRELLNE
YGFPGDDTPIVVGSALEVLENQDNAEKTKCIDELMEAIDSYIPTPERATDQPFLMPVEDVFTITGRGTVATGRVERGVLH
TGDEVELIGMKQEVSKTVCTGIEMFRKILDEAMAGDNIGALLRGIQRDEIQRGQVLAKPGSVTPHKKFVGQVYVLKKEEG
GRHTPFFNGYRPQFYFRTTDVTGSINLPEGVEMVMPGDHIDMAVELITPVAMHENLRFAIREGGRTVGSGVVTTISE

Sequences:

>Translated_397_residues
MAKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQKGGASATKYDEIDKAPEEKERGITINTSHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLASRVGVQYIVVFLNKADQVDDPELIELVEMEVRELLNE
YGFPGDDTPIVVGSALEVLENQDNAEKTKCIDELMEAIDSYIPTPERATDQPFLMPVEDVFTITGRGTVATGRVERGVLH
TGDEVELIGMKQEVSKTVCTGIEMFRKILDEAMAGDNIGALLRGIQRDEIQRGQVLAKPGSVTPHKKFVGQVYVLKKEEG
GRHTPFFNGYRPQFYFRTTDVTGSINLPEGVEMVMPGDHIDMAVELITPVAMHENLRFAIREGGRTVGSGVVTTISE
>Mature_396_residues
AKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQKGGASATKYDEIDKAPEEKERGITINTSHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLASRVGVQYIVVFLNKADQVDDPELIELVEMEVRELLNEY
GFPGDDTPIVVGSALEVLENQDNAEKTKCIDELMEAIDSYIPTPERATDQPFLMPVEDVFTITGRGTVATGRVERGVLHT
GDEVELIGMKQEVSKTVCTGIEMFRKILDEAMAGDNIGALLRGIQRDEIQRGQVLAKPGSVTPHKKFVGQVYVLKKEEGG
RHTPFFNGYRPQFYFRTTDVTGSINLPEGVEMVMPGDHIDMAVELITPVAMHENLRFAIREGGRTVGSGVVTTISE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=57.3979591836735, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=30.4250559284116, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=30.0448430493274, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=30.0448430493274, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4503475, Length=456, Percent_Identity=30.9210526315789, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI54607086, Length=326, Percent_Identity=30.6748466257669, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI46094014, Length=325, Percent_Identity=29.5384615384615, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018520, Length=339, Percent_Identity=28.9085545722714, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018522, Length=339, Percent_Identity=28.9085545722714, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194097354, Length=339, Percent_Identity=28.9085545722714, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4503507, Length=295, Percent_Identity=29.8305084745763, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI157426893, Length=239, Percent_Identity=27.6150627615063, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=34.0909090909091, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=36.697247706422, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=36.697247706422, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=36.697247706422, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=73.4177215189873, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=73.4177215189873, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=372, Percent_Identity=30.1075268817204, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789108, Length=327, Percent_Identity=29.3577981651376, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788922, Length=338, Percent_Identity=24.5562130177515, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789559, Length=239, Percent_Identity=32.2175732217573, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=150, Percent_Identity=32, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=51.25, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.6410256410256, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI17552884, Length=462, Percent_Identity=31.8181818181818, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=462, Percent_Identity=31.8181818181818, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=30.6818181818182, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=28.4064665127021, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=28.3720930232558, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=359, Percent_Identity=29.8050139275766, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=31.5614617940199, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=29.8319327731092, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=281, Percent_Identity=26.3345195729537, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=64.8241206030151, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=330, Percent_Identity=34.2424242424242, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=330, Percent_Identity=34.2424242424242, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=24.0534521158129, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=26.6862170087977, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=300, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=248, Percent_Identity=28.2258064516129, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=56.8877551020408, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=56.8877551020408, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.5181347150259, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=452, Percent_Identity=29.646017699115, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45553816, Length=452, Percent_Identity=29.646017699115, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651721, Length=452, Percent_Identity=29.646017699115, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17864154, Length=452, Percent_Identity=29.646017699115, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=29.5555555555556, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=29.5555555555556, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.9816513761468, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.8550724637681, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=30.8441558441558, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=28.4697508896797, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=28.4697508896797, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI78706572, Length=255, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CLOB1 (A7FZ71)

Other databases:

- EMBL:   CP000726
- EMBL:   CP000726
- RefSeq:   YP_001385798.1
- RefSeq:   YP_001385812.1
- ProteinModelPortal:   A7FZ71
- SMR:   A7FZ71
- STRING:   A7FZ71
- GeneID:   5396973
- GeneID:   5397314
- GenomeReviews:   CP000726_GR
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3539
- KEGG:   cba:CLB_3553
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   IAPIACE
- BioCyc:   CBOT441770:CLB_3539-MONOMER
- BioCyc:   CBOT441770:CLB_3553-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43484; Mature: 43353

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQKGGASATKYDEIDKAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCC
ITINTSHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLASRVGVQYIVVFLNKADQVDDPELIELVEMEVRELLNEYGFPGDDTPIVVGSALEVLE
HHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
NQDNAEKTKCIDELMEAIDSYIPTPERATDQPFLMPVEDVFTITGRGTVATGRVERGVLH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHEEEECCCEEEECCCCCCCCC
TGDEVELIGMKQEVSKTVCTGIEMFRKILDEAMAGDNIGALLRGIQRDEIQRGQVLAKPG
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHCCCCEEECCC
SVTPHKKFVGQVYVLKKEEGGRHTPFFNGYRPQFYFRTTDVTGSINLPEGVEMVMPGDHI
CCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEECCCCCCCCEEEECCCHH
DMAVELITPVAMHENLRFAIREGGRTVGSGVVTTISE
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure 
AKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQKGGASATKYDEIDKAPEEKERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCC
ITINTSHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLASRVGVQYIVVFLNKADQVDDPELIELVEMEVRELLNEYGFPGDDTPIVVGSALEVLE
HHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
NQDNAEKTKCIDELMEAIDSYIPTPERATDQPFLMPVEDVFTITGRGTVATGRVERGVLH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHEEEECCCEEEECCCCCCCCC
TGDEVELIGMKQEVSKTVCTGIEMFRKILDEAMAGDNIGALLRGIQRDEIQRGQVLAKPG
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHCCCCEEECCC
SVTPHKKFVGQVYVLKKEEGGRHTPFFNGYRPQFYFRTTDVTGSINLPEGVEMVMPGDHI
CCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEECCCCCCCCEEEECCCHH
DMAVELITPVAMHENLRFAIREGGRTVGSGVVTTISE
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA