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The map label for this gene is bacA [H]

Identifier: 170755763

GI number: 170755763

Start: 2763498

End: 2765327

Strand: Reverse

Name: bacA [H]

Synonym: CLD_1978

Alternate gene names: 170755763

Gene position: 2765327-2763498 (Counterclockwise)

Preceding gene: 170754699

Following gene: 170755376

Centisome position: 69.86

GC content: 25.96

Gene sequence:

>1830_bases
ATGAGCTATAACTATTTTGAAAAACAACAAAGAGAAGATTCTATATTGGTTGGACAGGATATTTTAAGTTTTAGATATAA
TAATAGCAGAATAGATACTACAATAATTTCTTGCTTAAGAAAAATCGTTAACTTAAGCAAAGAAAAAGTAGCTATTATAG
ATAATGAAACAAAGATAACTTATGGACATTTCATGAAGCGAATTAATGCTCTATCAAATTTTTTGCTGAGTCAAAAGGAA
TTTGAAAGAACATTACCAATTACTTTAATTTTAAATGGGAATTATTTAAGATATGTTTGCGTATATGGTGTTTTAGCTTC
AGGTGGGTATTATTTGCCTATAGAGCCAGAAGTAAACAATATTAATAGAATAATTTCTTTGGTAGAACGTTCTAAAAGTA
AAGTTGTAATTACAAATAGAGAGTATGTAAATATATTAAAAGAAAAAATATCAGAAAGTGTAAATCTTATAATTATAGAA
GATTTAGATTTTAGTAGCCTAGATAGCAAAGAAAACGTTATTGATATAAAAGCTGATGATCCAGCATATATGATATATAC
ATCTGGATCTACGGGAGTGCCTAAAGGTGTTGTTATACCTCATAGAGCTGTTGTGAATTTAGTTAATGCATTGTACGTTA
ATTTTAGATTAACAAAAAAGGACAGAGGTGTGGCGTTTACATCATTTTCTTTTGATTCATGTGGTTGTGATATATATACA
TATTTATTGAATGAAGTCCCTTTAGTATGTATTGAAAAGAGAAAAGAATATGTTTACGATATTGAAAAATTAAATAAATT
TTGTATCGATAATATGATATCAATAATGTTTTTACCTACGGTTTTAGCTGAAAAATTTATTACAGTTGATAATAATGTGC
TAAGAGTTCTTTATTTTGGAGGGGAAAAATTTTGTAAGAATAAAGTTACTAATTACGAGTTATTTAATTCTTATGGATTG
ACAGAAACAGGAATACTTAATACAGTCTATAAGATTAAAGGAGATGAGCGAGAAATTCCTTTGGGAGATCCAATAATAAA
TTCAGGAATATTAATTTTAGATGATCAAGATAAAATTATACCTAAAGGGAAATTAGGAGAAATTGCAATTTTTGGTTCTG
GTATAGCAATAGAATATGATAATAATACAGAGTTGACTAATAAAAAATTTATTAAGTTAAAAGAAATGGGTAATTTGAGA
TGTTGTAAAACTGGAGATTTGGGATATATAAATGAGAATGATAATCTATATTTTAAGGGTAGAAAAGATAAACAAGTAAA
AATAAGTGGTTACAGGATAGAGTTAGATGAGATACGATTTTATTTGCAGAAATATGCTTGCATAGAGTTAAGTATTCCTA
TGGTAATAAAAAAAGGTAAATTATCCTTATTAGTTAATTTTTATGTTGCAAAAAAAGTACTTGATGAAGAGTTAATTAAT
AAATTTTTAAGTAAATATTTACCTTTTTATATGGTGCCATTATGTCACATTAACATAAAAGATGTTCCATATACTACAAA
TGGAAAAGTTGATTATAATAGGTTGCAGAAAATTTTTGAGAATAAAAAAGAAAAAGTATATCTAAAAAGTAATTTACTGT
ATAGCAAATTAGAATTAAAAGAGTTCCTAATTGATACTTATGCCAATATACTTAAAATGGATAAAGATGAAATAATGGAA
GATGGTAGTTTCTTTGAAATGGGTGGAGACTCACTTAAAGCTATGAAATTACAATGGTATATACAAGATGAATTAGGTGT
AGAAGTAGACATATCTAAAATTTATGAAGAATTCAGTATAAAAAAACTCCTAAATTTAATACTAATATGA

Upstream 100 bases:

>100_bases
GCTTATTTAGAAAAAAAATAGATGAAATTTTAATGATTAAAGAAATTATAAATAATAAGTAAAAATGTGAGTTATATAAA
AATAATTAGGAGAAAAAAGG

Downstream 100 bases:

>100_bases
TTATATCCAAGTTATTCTATTTTCAAGGAGGTGTGATGGTGTGAGTATAGTTCGATTATTTACATTACCAGGGGCGGGTT
CTTCAGCAATAATGTATTAC

Product: putative nonribosomal peptide synthetase

Products: NA

Alternate protein names: BA1; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; ATP-dependent cysteine adenylase; CysA; Cysteine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase; ATP-dependent glutamate adenylase; GluA; Glutamate activase; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; Glutamate racemase [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MSYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLSQKE
FERTLPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVNLIIIE
DLDFSSLDSKENVIDIKADDPAYMIYTSGSTGVPKGVVIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYT
YLLNEVPLVCIEKRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGL
TETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLR
CCKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKKVLDEELIN
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYNRLQKIFENKKEKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIME
DGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI

Sequences:

>Translated_609_residues
MSYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLSQKE
FERTLPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVNLIIIE
DLDFSSLDSKENVIDIKADDPAYMIYTSGSTGVPKGVVIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYT
YLLNEVPLVCIEKRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGL
TETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLR
CCKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKKVLDEELIN
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYNRLQKIFENKKEKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIME
DGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI
>Mature_608_residues
SYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLSQKEF
ERTLPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVNLIIIED
LDFSSLDSKENVIDIKADDPAYMIYTSGSTGVPKGVVIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYTY
LLNEVPLVCIEKRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGLT
ETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRC
CKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKKVLDEELINK
FLSKYLPFYMVPLCHINIKDVPYTTNGKVDYNRLQKIFENKKEKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIMED
GSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI

Specific function: Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=594, Percent_Identity=24.2424242424242, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1786801, Length=608, Percent_Identity=23.6842105263158, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI221142682, Length=453, Percent_Identity=24.2825607064018, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788107, Length=338, Percent_Identity=23.9644970414201, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=22.4899598393574, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17550940, Length=441, Percent_Identity=25.3968253968254, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556356, Length=424, Percent_Identity=23.3490566037736, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=22.9674796747967, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=368, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=609, Percent_Identity=23.6453201970443, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=24.8618784530387, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648676, Length=580, Percent_Identity=23.448275862069, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013624
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF08415 NRPS; PF00550 PP-binding [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 70275; Mature: 70144

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKIT
CCCCCCHHHCCCCCEEECCHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
YGHFMKRINALSNFLLSQKEFERTLPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNN
HHHHHHHHHHHHHHHHCHHHHHCCCCEEEEECCCEEEHHHHHHHHHCCCEEEECCCCHHH
INRIISLVERSKSKVVITNREYVNILKEKISESVNLIIIEDLDFSSLDSKENVIDIKADD
HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCC
PAYMIYTSGSTGVPKGVVIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYT
CEEEEEECCCCCCCCCEECCHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCHHHHH
YLLNEVPLVCIEKRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFG
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEEC
GEKFCKNKVTNYELFNSYGLTETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKII
CHHHHHCCCCCEEEECCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC
PKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRCCKTGDLGYINENDNLYFKG
CCCCCCEEEEEECCEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEEC
RKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKKVLDEELIN
CCCCEEEEEEEEEEHHHHHHHHHHHEEEEEECCEEEECCCCEEEHHHHHHHHHHHHHHHH
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYNRLQKIFENKKEKVYLKSNLLYSKLELK
HHHHHHCHHHEEHEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHH
EFLIDTYANILKMDKDEIMEDGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSI
HHHHHHHHHHHHCCHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCEEEHHHHHHHHHH
KKLLNLILI
HHHHHHHCC
>Mature Secondary Structure 
SYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKIT
CCCCCHHHCCCCCEEECCHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
YGHFMKRINALSNFLLSQKEFERTLPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNN
HHHHHHHHHHHHHHHHCHHHHHCCCCEEEEECCCEEEHHHHHHHHHCCCEEEECCCCHHH
INRIISLVERSKSKVVITNREYVNILKEKISESVNLIIIEDLDFSSLDSKENVIDIKADD
HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCC
PAYMIYTSGSTGVPKGVVIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYT
CEEEEEECCCCCCCCCEECCHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCHHHHH
YLLNEVPLVCIEKRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFG
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEEC
GEKFCKNKVTNYELFNSYGLTETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKII
CHHHHHCCCCCEEEECCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC
PKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRCCKTGDLGYINENDNLYFKG
CCCCCCEEEEEECCEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEEC
RKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKKVLDEELIN
CCCCEEEEEEEEEEHHHHHHHHHHHEEEEEECCEEEECCCCEEEHHHHHHHHHHHHHHHH
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYNRLQKIFENKKEKVYLKSNLLYSKLELK
HHHHHHCHHHEEHEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHH
EFLIDTYANILKMDKDEIMEDGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSI
HHHHHHHHHHHHCCHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCEEEHHHHHHHHHH
KKLLNLILI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9427658 [H]